Effect of Removing Seminal Plasma Using a Sperm Filter on the Viability of Refrigerated Stallion Semen

Cooling of equine semen obtained from some stallions results in lower seminal quality and viability when the seminal plasma (SP) is present. The objective of this study was to evaluate the effect of the removal of SP using a Sperm Filter on the viability of cooled stallion semen. For this purpose, 31 stallions were used. Their ejaculates were divided into three groups: CN, semen was diluted with an extender; FLT, SP was removed by filtration; and CT, SP was removed by centrifugation and cooled to 15°C for 24 hours. Sperm kinetics and plasma membrane integrity were evaluated immediately after collection (T0) and after 24 hours of refrigeration (T1). No difference (P > .05) was noted at T1 for total sperm motility (TM), progressive sperm motility, or plasma membrane integrity when semen samples from all the stallions were analyzed. However, when samples from stallions termed bad coolers were analyzed (TM = <30% at T1), a difference was observed in TM and progressive sperm motility for CN compared with FLT and CT at T1. Sperm recovery was greater when SP was removed using the filter (FLT) to that when the SP was removed by centrifugation (CN) (89% vs. 81%). Thus, we concluded that filtering with a Sperm Filter is an efficient and practical method for removal of SP from stallion ejaculates, with lower sperm loss than centrifugation. We also found that the presence of SP reduces the quality and viability of cooled semen from stallions whose semen is sensitive to the process of refrigeration. © 2013 Elsevier Inc.

Estudo do efeito da adição de frustração no enovelamento de proteínas utilizando modelos baseados em estruturas

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Prospecção das interações mastoparano-membrana em proteolipossomos como modelo para o desenvolvimento racional de novos agentes antimicrobianos

Pós-graduação em Ciências Biológicas (Biologia Celular e Molecular) - IBRC

Delineamentos D-ótimos para os modelos de Michaelis-Menten e de Hill

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Efeito das condições de congelamento sobre atributos de qualidade de fatias de abacaxi liofilizado

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Clarificação por flotação com ar dissolvido (FAD) da calda de açúcar cristal para produção de açúcar refinado

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Efeito da velocidade de congelamento sobre a liofilização, reidratação e atributos de qualidade de fatias de maçã

Pós-graduação em Engenharia e Ciência de Alimentos - IBILCE

Desidratação osmótica e secagem de maçãs: I. Comportamento do tecido em soluções osmóticas. II. Modelagem matemática de difusão

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Distribuição estatística de um indexo econômico em mercado emergente - Bolsa de valores de São Paulo (IBOVESPA)

Pós-graduação em Física - IGCE

Análise das células inflamatórias e expressão da proteína anti-inflamatória anexina A1 em pacientes com endometriose

Pós-graduação em Genética - IBILCE

Avaliação do efeito inflamatório da proteína galectina-1 nas células epiteliais pigmentadas da retina humana (ARPE-19) após ativação por endotoxina

Pós-graduação em Genética - IBILCE

Fatores operacionais e cinética do processo fermentativo para otimização da produção de etanol em escala industrial

Pós-graduação em Microbiologia Agropecuária - FCAV

Compartimentos de K, Ca e Mg em solos e sua relação com o crescimento inicial, fisiologia e produtividade do eucalipto

Forestry has grown in a continuous and accelerated manner in Brazil, constituting a strategic activity for the generation of employment, income and tributes, favoring social and economic development of Brazilian agribusiness. The objectives of this study were: (1) evaluate the contents of K, Ca and Mg in the reserve compartments, non-interchangeable, interchangeable, available and the speed of its release, its correlations and its effects over productivity (annual average increment – AAI) of eucalyptus plantations, in forest sites cultivated in soils of the state of Rio Grande do Sul; (2) evaluate the initial growth, nutrition and physiological aspects of eucalyptus plants, cultivated with and without the addition of mineral sources of potassium (K), calcium (Ca) and magnesium (MG), in soils obtained from forest sites in the state of Rio Grande do Sul. In the first study, contents of K, Ca and Mg were evaluated in sulfuric digestion extract, boiling nitric acid, ammonium chloride, Mehlich-1 (only K), potassium chloride (Ca and Mg), as well as the release speed of these nutrients in the soil. In the second study, growth variables, nutritional aspects, photosynthetic rate (A) and transpiration rate of the plants (E) grown in distinct soils were evaluated under controlled conditions. The contents of K, Ca and Mg varied between compartments and depths in the studied soil classes, with the highest proportions found in the reserve compartment, indicating the importance of this compartment for the supplement of these nutrients at average and long terms. The great majority of K, Ca and Mg compartments presented significant correlations between each other, showing the dependence between them and the importance of evaluating the contents of these nutrients in the different compartments to adapt the nutritional management of the plants to each soil class, and to obtain continuous productions, minimizing the negative effects to the environment. Plants cultivated in soils that present larger reserves, availability and K, Ca and Mg release kinetics, presented similar height (H), stem diameter (SD) and shoot dry mass (SDM), with or without fertilization with K, Ca and Mg. The plants presented higher leaf content and accumulation of K in all soils fertilized with K, Ca and Mg.

Avaliação comparativa do sêmen ovino refrigerado nos meios Glicina-Gema-Leite, Glicina-Gema purificada-Leite e Glicina-extrato de lipoproteínas de baxa densidade-Leite

Pós-graduação em Medicina Veterinária - FMVZ

A interferência da luminosidade na agressividade e na hierarquia social de ciclídeos

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)