Modelling staphylococcal pneumonia in a human 3D lung tissue model system delineates toxin-mediated pathology

Staphylococcus aureus necrotizing pneumonia is recognized as a toxin-mediated disease, yet the tissue-destructive events remain elusive, partly as a result of lack of mechanistic studies in human lung tissue. In this study, a three-dimensional (3D) tissue model composed of human lung epithelial cells and fibroblasts was used to delineate the role of specific staphylococcal exotoxins in tissue pathology associated with severe pneumonia. To this end, the models were exposed to the mixture of exotoxins produced by S. aureus strains isolated from patients with varying severity of lung infection, namely necrotizing pneumonia or lung empyema, or to purified toxins. The necrotizing pneumonia strains secreted high levels of alpha-toxin and Panton-Valentine leukocidin (PVL), and triggered high cytotoxicity, inflammation, necrosis and loss of E-cadherin from the lung epithelium. In contrast, the lung empyema strain produced moderate levels of PVL, but negligible amounts of alpha-toxin, and triggered limited tissue damage. alpha-toxin had a direct damaging effect on the epithelium, as verified using toxin-deficient mutants and pure alpha-toxin. Moreover, PVL contributed to pathology through the lysis of neutrophils. A combination of alpha-toxin and PVL resulted in the most severe epithelial injury. In addition, toxin-induced release of pro-inflammatory mediators from lung tissue models resulted in enhanced neutrophil migration. Using a collection of 31 strains from patients with staphylococcal pneumonia revealed that strains producing high levels of alpha-toxin and PVL were cytotoxic and associated with fatal outcome. Also, the strains that produced the highest toxin levels induced significantly greater epithelial disruption. Of importance, toxin-mediated lung epithelium destruction could be inhibited by polyspecific intravenous immunoglobulin containing antibodies against alpha-toxin and PVL. This study introduces a novel model system for study of staphylococcal pneumonia in a human setting. The results reveal that the combination and levels of alpha-toxin and PVL correlate with tissue pathology and clinical outcome associated with pneumonia.

Manual de crianza, raza, entrenamiento y reglamento del gallo de combate

Los gallos de combate llegaron a América provenientes de Europa; los conquistadores españoles trajeron gallos del tipo bankivoyde; malayoide, entre otros. Los primeros gallos de combate se originaron hace más de cinco mil años, ascendientes de los que hoy conocemos como Asiles Las primeras peleas de gallo se registraron en la India tres mil años antes de Cristo. Posiblemente las primeras aves que se pelearon por separado, fueron machos nacidos en cautiverio, tanto Malayos (Gallus giganteus)como Bankivas (subespecies de Gallus gallus). Para la selección y formación del "gallo de combate" intervinieron una serie de factores, los cuales se tejieron alrededor de la antigua Bélica de estas aves, de allí vendría el interés por crearlos, teniendo antes que capturarlo y trasladarlo desde su hábitat natural (los bosques) al cautiverio,cuando eran pollitos. Seguramente el primer criador de gallos de combate se daría cuenta de la actitud Bélica conflictiva en los pollones; quienes, al crecer demostrarían sus primeras actitudes de luchadores, matándose unos a otros. Esto forzaría a los criadores a separarlos en pequeñas jaulas rústicas. Luego hicieron la primera selección de los mejores ejemplares, según las actitudes de lucha, fuerza, velocidad, agilidad, entre otras. Antes de empezar con la explicación del manejo de los gallos de pelea, es necesario ejecutar algunas aclaraciones con relación a lo que son los gallos de pelea, y sobre todo, quienes son los que se encargan de su manejo. Cabe entender que, no todas las personas que están en este medio son iguales, y la forma más sencilla de explicar esto, es marcar la diferencia entre "galleros" y "criadores de gallos" Estos últimos, para tener aves de calidad, le es indispensable saber eleccionar a los reproductores, tal vez esta es la tarea más difícil de todas. Fundamentar la base para una buena alimentación, sana y racional. Es muy importante conocer sobre el tema de las instalaciones, para los que quieren iniciar su criadero de aves de combate. Deben tomarse en cuenta, en el manejo del gallo de pelea, las prácticas adecuadas del descreste y tusado. Las enfermedades se reconocen por sus señales muy claras. Las vacunas es el método más sencillo y más barato de prevenir pérdidas. El entrenamiento o cuido apropiado y la buena alimentación del gallo, son esenciales para obtener victorias o buen rendimiento durante el combate, si falta uno de éstos dos, puede ser fatal para sus gallos. Existen distintos tipos de entrenamientos para un gallo de pelea, ninguno es criticable, pero definitivamente es necesario preguntarse ¿Están ganando con este método? En síntesis hay que identificar y evaluar, comenzando desde el lugar en dónde desarrollar nuestras crías, depende mucho de las condiciones climáticas y ambientales(humedad, sol, temperatura, disposición de agua fresca, entre otras). Disponibilidad de materia prima como granos, alimento balanceado, y forraje verde. Disposición de personal calificado en la temática y permanencia continúa.

Familia romana e identidad femenina en época de Augusto

Resumen: Hacia el 167 a.C., un joven Polibio, se sorprendía de que Roma hubiera podido afi rmar su poderío en la península, resistir los ataques cartagineses y acabar imponiendo sus leyes en Oriente. Probablemente –explica P. Grimal– en ello también haya infl uido que las costumbres y tradiciones romanas contribuían a asegurar una superioridad sobre el resto de las culturas y hacían de Roma una ciudad única entre todas. La familia y la mujer constituyeron parte fundamental dentro de la consolidación de esa estructura socio política y de ello heredaríamos, especialmente a nivel normativo, una sólida tradición jurídico-romana. Sin embargo, la época imperial representó un grave período de crisis en el cual, tomar esposa, dar hijos a la patria y controlar los patrimonios, se convirtió en una importante reafi rmación moral, jurídica y política para la sociedad de su tiempo. En esta forma, se analizarán brevemente algunos conceptos y leyes relevantes –cuyos ecos volveremos a encontrar refl ejados a lo largo y ancho de la España cristiana medieval– los cuales nos ayudarán a adentrarnos en la cotidianidad de este singular período, sus emancipadas mujeres aristocráticas y el derecho privado.

Bioética personalista y familia

En este trabajo se aborda la visión del hombre como persona humana y el respeto que se le debe como tal desde el inicio de su vida en la concepción hasta su fin natural. Los atentados que pueden y podrán cometerse contra la vida constituyen otros tantos atentados también contra la institución familiar, lugar natural del nacimiento y desarrollo de la vida de un nuevo ser humano. El respeto por el hombre en cuanto persona es una de las exigencias que no admiten discusión; de ella dependen la dignidad y también el bienestar y la subsistencia de la Humanidad.

La propaganda por el hecho dentro del discurso anarquista en la Argentina del Centenario

Dueños de un discurso contestatario y crítico, pero a la vez formativo y comprometido con el hombre trabajador como centro de sus proyectos, el principal objetivo del movimiento anarquista argentino fue impugnar, cuestionar y contradecir el discurso oficialista. Una de las tácticas que utilizaron los militantes libertarios fue la propaganda por el hecho o acción directa, como denominaremos los actos terroristas ácratas. Si bien en la Argentina encontramos una importante literatura libertaria con un lenguaje perturbador y radicalizado, los sucesos violentos fueron aislados, aunque no por ello dejaron de preocupar tanto a la clase dirigente como a la sociedad en general, que llegó a identificar al militante ácrata con un criminal y lo transformó en un problema de higiene y seguridad pública. Esta situación, sumada no sólo al tenor violento que caracterizó los conflictos laborales, sino también a las noticias nada tranquilizadoras que llegaban de Europa, donde una ola de atentados sacudió la opinión pública, provocaron un pánico exagerado en los distintos ambientes sociales. La consecuencia directa fue la celebración del Centenario en medio de una oleada de huelgas, protestas y con declaración del estado de sitio.

Terrorism and the World Economy

Published as an article in: European Economic Review, 2008, vol. 52, issue 1, pages 1-27.

El poder educativo de la televisión : un discurso paralelo a la escuela

[Es] En este trabajo se parte de la hipótesis de que los propios contenidos televisivos son fuente de aprendizaje a través de las narraciones televisivas. En concreto, defendemos la idea de que es posible enseñar y aprender valores a través de dichas narraciones. Se sistematizan algunas de las investigaciones que han abordado la relación entre televisión y valores desde una triple dimensión: los propios contenidos; el medio en sí mismo y el lenguaje. Esta revisión nos permite defender que las condenas reiteradas a la televisión, responsabilizándola de la mayoría de los males que sufre la juventud, no pueden apoyarse en los estudios que la psicología ha realizado en las últimas décadas. Entendemos que el telespectador incorpora la información televisiva desde distintos contextos y que la enculturación no es unidireccional. Existe una interacción entre contextos de desarrollo y mensajes. Concretamente nos interesa analizar los valores implícitos y explícitos que subyacen a los contenidos televisivos. Para ello basándonos en el modelo elaborado por Schwartz y Bilsky hemos creado un cuestionario (Val-TV 01) con el fin de clasificar los valores e interpretar las conductas visualizadas en los textos televisivos y relacionarlos con los propios valores de los adolescentes.

Analysis of sea turtle bycatch in the commercial shrimp fisheries of Southeast U.S. waters and the Gulf of Mexico

The coastal shrimp trawl fisheries have long been the focus of conservation actions to reduce turtle bycatch and mortality in the Gulf of Mexico and the U.S. Atlantic (NRC, 1990). Calculation of catch rates of sea turtles in shrimp trawls is necessary to evaluate the impact on sea turtle populations. In this paper we analyze sea turtle bycatch to provide an estimate of the current number of interactions with otter trawl gear as well as an estimate of the number of fatal inions in Southeast U.S. waters and the Gulf of Mexico. We also provide an estimate of the number of individuals likely to die in the future with the new regulations that will require an increase in the size of the escape openings in trutle excluder devices (TEDs). The new regulations will allow many more turtles to escape. Other gears also are discussed. (PDF contains 24 pages)

El mito de Safo en el relato decadente

[FR] La femme homosexuelle, reflet d’un désir mythique hermaphrodite, est, pendant la fin du siècle une figure littéraire paradoxale et en même temps obsédante. Sapho est, comme la femme fatale, une des protagonistes de la fin-du-siècle qui répond à une misogynie récurrente dans l’imaginaire créateur. Il s’agit d’un désir artificiel, éros intangible (Péladan 1910: 74), contre nature, un idéal impossible: Sexe initial,sexe définitif, absolu de l’amour, absolu de la forme, sexe qui nies le sexe, sexe d’éternité! Los à toi, Androgyne! (Péladan 1910: 76). Sapho, figure archétypique, androgyne, constitue en définitive le symbole de l’abolition des sexes, de la confusion des genres, de la crise du couple traditionnel. Elle met en évidence la solitude, la peur et un désir inassouvi.

Increased opioid dependence in a mouse model of panic disorder

[EN] Panic disorder is a highly prevalent neuropsychiatric disorder that shows co-occurrence with substance abuse. Here, we demonstrate that TrkC, the high-affinity receptor for neurotrophin-3, is a key molecule involved in panic disorder and opiate dependence, using a transgenic mouse model (TgNTRK3). Constitutive TrkC overexpression in TgNTRK3 mice dramatically alters spontaneous firing rates of locus coeruleus (LC) neurons and the response of the noradrenergic system to chronic opiate exposure, possibly related to the altered regulation of neurotrophic peptides observed. Notably, TgNTRK3 LC neurons showed an increased firing rate in saline-treated conditions and profound abnormalities in their response to met5-enkephalin. Behaviorally, chronic morphine administration induced a significantly increased withdrawal syndrome in TgNTRK3 mice. In conclusion, we show here that the NT-3/TrkC system is an important regulator of neuronal firing in LC and could contribute to the adaptations of the noradrenergic system in response to chronic opiate exposure. Moreover, our results indicate that TrkC is involved in the molecular and cellular changes in noradrenergic neurons underlying both panic attacks and opiate dependence and support a functional endogenous opioid deficit in panic disorder patients.

Conservation Biology of Elasmobranchs

Elasmobranchs are vital and valuable components of the marine biota. From an ecological perspective they occupy the role of top predators within marine food webs, providing a regulatory control that helps balance the ecosystem. From an evolutionary perspective, this group represents an early divergence along the vertebrate line that produced many unusual, but highly successful, adaptations in function and form. From man's perspective, elasmobranchs have been considered both an unavoidable nuisance, and an exploitable fishery resource. A few of the large shark species have earned a dubious notoriety because of sporadic attacks on humans that occur in coastal areas each year worldwide; the hysteria surrounding an encounter with a shark can be costly to the tourist industry. More importantly, elasmobranchs are often considered a detriment to commercial fishing operations; they cause significant economic damage to catches and fishing gear. On the other hand, consumer attitudes have changed concerning many previously unpopular food fishes, including elasmobranchs, and this group of fishes has been increasingly used by both recreational and commercial fishing interests. Many elasmobranchs have become a popular target of recreational fishermen for food and sport because of their abundance, size, and availability in coastal waters. Similarly, commercial fisheries for elasmobranchs have developed or expanded from an increased demand for elasmobranch food products. (PDF file contains 108 pages.)

A novel form of human disease with a protease-sensitive prion protein and heterozygosity methionine/valine at codon 129: Case report

Background: Sporadic Creutzfeldt-Jakob disease (sCJD) is a rare neurodegenerative disorder in humans included in the group of Transmissible Spongiform Encephalopathies or prion diseases. The vast majority of sCJD cases are molecularly classified according to the abnormal prion protein (PrPSc) conformations along with polymorphism of codon 129 of the PRNP gene. Recently, a novel human disease, termed "protease-sensitive prionopathy", has been described. This disease shows a distinct clinical and neuropathological phenotype and it is associated to an abnormal prion protein more sensitive to protease digestion. Case presentation: We report the case of a 75-year-old-man who developed a clinical course and presented pathologic lesions compatible with sporadic Creutzfeldt-Jakob disease, and biochemical findings reminiscent of "protease-sensitive prionopathy". Neuropathological examinations revealed spongiform change mainly affecting the cerebral cortex, putamen/globus pallidus and thalamus, accompanied by mild astrocytosis and microgliosis, with slight involvement of the cerebellum. Confluent vacuoles were absent. Diffuse synaptic PrP deposits in these regions were largely removed following proteinase treatment. PrP deposition, as revealed with 3F4 and 1E4 antibodies, was markedly sensitive to pre-treatment with proteinase K. Molecular analysis of PrPSc showed an abnormal prion protein more sensitive to proteinase K digestion, with a five-band pattern of 28, 24, 21, 19, and 16 kDa, and three aglycosylated isoforms of 19, 16 and 6 kDa. This PrPSc was estimated to be 80% susceptible to digestion while the pathogenic prion protein associated with classical forms of sporadic Creutzfeldt-Jakob disease were only 2% (type VV2) and 23% (type MM1) susceptible. No mutations in the PRNP gene were found and genotype for codon 129 was heterozygous methionine/valine. Conclusions: A novel form of human disease with abnormal prion protein sensitive to protease and MV at codon 129 was described. Although clinical signs were compatible with sporadic Creutzfeldt-Jakob disease, the molecular subtype with the abnormal prion protein isoforms showing enhanced protease sensitivity was reminiscent of the "protease-sensitive prionopathy". It remains to be established whether the differences found between the latter and this case are due to the polymorphism at codon 129. Different degrees of proteinase K susceptibility were easily determined with the chemical polymer detection system which could help to detect proteinase-susceptible pathologic prion protein in diseases other than the classical ones.

Donut-Shaped Fingerprint In Homologous Polypeptide Relationships-A Topological Feature Related To Pathogenic Structural Changes In Conformational Disease

Features of homologous relationship of proteins can provide us a general picture of protein universe, assist protein design and analysis, and further our comprehension of the evolution of organisms. Here we carried Out a Study of the evolution Of protein molecules by investigating homologous relationships among residue segments. The motive was to identify detailed topological features of homologous relationships for short residue segments in the whole protein universe. Based on the data of a large number of non-redundant Proteins, the universe of non-membrane polypeptide was analyzed by considering both residue mutations and structural conservation. By connecting homologous segments with edges, we obtained a homologous relationship network of the whole universe of short residue segments, which we named the graph of polypeptide relationships (GPR). Since the network is extremely complicated for topological transitions, to obtain an in-depth understanding, only subgraphs composed of vital nodes of the GPR were analyzed. Such analysis of vital subgraphs of the GPR revealed a donut-shaped fingerprint. Utilization of this topological feature revealed the switch sites (where the beginning of exposure Of previously hidden "hot spots" of fibril-forming happens, in consequence a further opportunity for protein aggregation is Provided; 188-202) of the conformational conversion of the normal alpha-helix-rich prion protein PrPC to the beta-sheet-rich PrPSc that is thought to be responsible for a group of fatal neurodegenerative diseases, transmissible spongiform encephalopathies. Efforts in analyzing other proteins related to various conformational diseases are also introduced. (C) 2009 Elsevier Ltd. All rights reserved.

INTERPOL's surveillance network in curbing transnational terrorism

This paper investigates the role that INTERPOL surveillance – the Mobile INTERPOL Network Database (MIND) and the Fixed INTERPOL Network Database (FIND) – played in the War on Terror since its inception in 2005. MIND/FIND surveillance allows countries to screen people and documents systematically at border crossings against INTERPOL databases on terrorists, fugitives, and stolen and lost travel documents. Such documents have been used in the past by terrorists to transit borders. By applying methods developed in the treatment-effects literature, this paper establishes that countries adopting MIND/FIND experienced fewer transnational terrorist attacks than had they not adopted MIND/FIND. Our estimates indicate that, on average, during 2008–2011, adopting and using MIND/FIND results in 1.23 fewer transnational terrorist incidents each year per 100 million people. Thus, a country like France with a population just above 64 million people in 2008 would have 0.79 fewer transnational terrorist incidents per year owing to its use of INTERPOL surveillance. For most treatment countries, this amounts to a sizeable proportional reduction of about 60 per cent.

Legal dimensions of the Chinese experience in Los Angeles, 1860-1880

On October 24, 1871, a massacre of eighteen Chinese in Los Angeles brought the small southern California settlement into the national spotlight. Within a few days, news of this “night of horrors” was reported in newspapers across the country. This massacre has been cited in Asian American narratives as the first documented outbreak of ethnic violence against a Chinese community in the United States. This is ironic because Los Angeles’ small population has generally placed it on the periphery in historical studies of the California anti-Chinese movement. Because the massacre predated Los Angeles’ organized Chinese exclusion movements of the late 1870s, it has often been erroneously dismissed as an aberration in the history of the city.

The violence of 1871 was an outburst highlighting existing community tensions that would become part of public debate by decade’s close. The purpose of this study is to insert the massacre into a broader context of anti-Chinese sentiments, legal discrimination, and dehumanization in nineteenth century Los Angeles. While a second incident of widespread anti-Chinese violence never occurred, brutal attacks directed at Chinese small businessmen and others highlighted continued community conflict. Similarly, economic rivalries and concerns over Chinese prostitution that underlay the 1871 massacre were manifest in later campaigns of economic discrimination and vice suppression that sought to minimize Chinese influence within municipal limits. An analysis of the massacre in terms of anti-Chinese legal, social and economic strategies in nineteenth-century Los Angeles will elucidate these important continuities.