Relationship between prion propensity and the rates of individual molecular steps of fibril assembly.

Peptides and proteins possess an inherent propensity to self-assemble into generic fibrillar nanostructures known as amyloid fibrils, some of which are involved in medical conditions such as Alzheimer disease. In certain cases, such structures can self-propagate in living systems as prions and transmit characteristic traits to the host organism. The mechanisms that allow certain amyloid species but not others to function as prions are not fully understood. Much progress in understanding the prion phenomenon has been achieved through the study of prions in yeast as this system has proved to be experimentally highly tractable; but quantitative understanding of the biophysics and kinetics of the assembly process has remained challenging. Here, we explore the assembly of two closely related homologues of the Ure2p protein from Saccharomyces cerevisiae and Saccharomyces paradoxus, and by using a combination of kinetic theory with solution and biosensor assays, we are able to compare the rates of the individual microscopic steps of prion fibril assembly. We find that for these proteins the fragmentation rate is encoded in the structure of the seed fibrils, whereas the elongation rate is principally determined by the nature of the soluble precursor protein. Our results further reveal that fibrils that elongate faster but fracture less frequently can lose their ability to propagate as prions. These findings illuminate the connections between the in vitro aggregation of proteins and the in vivo proliferation of prions, and provide a framework for the quantitative understanding of the parameters governing the behavior of amyloid fibrils in normal and aberrant biological pathways.

High temperature direct double side cooled inverter module for hybrid electric vehicle application

In this paper a novel approach to the design and fabrication of a high temperature inverter module for hybrid electrical vehicles is presented. Firstly, SiC power electronic devices are considered in place of the conventional Si devices. Use of SiC raises the maximum practical operating junction temperature to well over 200°C, giving much greater thermal headroom between the chips and the coolant. In the first fabrication, a SiC Schottky barrier diode (SBD) replaces the Si pin diode and is paired with a Si-IGBT. Secondly, double-sided cooling is employed, in which the semiconductor chips are sandwiched between two substrate tiles. The tiles provide electrical connections to the top and the bottom of the chips, thus replacing the conventional wire bonded interconnect. Each tile assembly supports two IGBTs and two SBDs in a half-bridge configuration. Both sides of the assembly are cooled directly using a high-performance liquid impingement system. Specific features of the design ensure that thermo-mechanical stresses are controlled so as to achieve long thermal cycling life. A prototype 10 kW inverter module is described incorporating three half-bridge sandwich assemblies, gate drives, dc-link capacitance and two heat-exchangers. This achieves a volumetric power density of 30W/cm3.

An experimental study on the load carrying capacity of cold-formed steel studs and panels

A full-scale experimental study on the structural performance of load-bearing wall panels made of cold-formed steel frames and boards is presented. Six different types of C-channel stud, a total of 20 panels with one middle stud and 10 panels with two middle studs were tested under vertical compression until failure. For panels, the main variables considered are screw spacing (300 mm, 400 mm, or 600 mm) in the middle stud, board type (oriented strand board - OSB, cement particle board - CPB, or calcium silicate board - CSB), board number (no sheathing, one-side sheathing, or two-side sheathing), and loading type (1, 3, or 4-point loading). The measured load capacity of studs and panels agrees well with analytical prediction. Due to the restraint by rivet connections between stud and track, the effective length factor for the middle stud and the side stud in a frame (unsheathed panel) is reduced to 0.90 and 0.84, respectively. The load carrying capacity of a stud increases significantly whenever one- or two-side sheathing is used, although the latter is significantly more effective. It is also dependent upon the type of board used. Whereas panels with either OSB or CPB boards have nearly identical load carrying capacity, panels with CSB boards are considerably weaker. Screw spacing affects the load carrying capacity of a stud. When the screw spacing on the middle stud in panels with one-side sheathing is reduced from 600 mm to 300 mm, its load carrying capacity increases by 14.5 %, 20.6% and 94.2% for OSB, CPB and CSB, respectively.

Directly synthesized strong, highly conducting, transparent single-walled carbon nanotube films

We report the direct synthesis of strong, highly conducting, and transparent single-walled carbon nanotube (SWNT) films. Systematically, tests reveal that the directly synthesized films have superior electrical and mechanical properties compared with the films made from a solution-based filtration process: the electrical conductivity is over 2000 S/cm and the strength can reach 360 MPa. These values are both enhanced by more than 1 order. We attribute these intriguing properties to the good and long interbundle connections. Moreover, by the help of an extrapolated Weibull theory, we verify the feasibility of reducing the interbundle slip by utilizing the long-range intertube friction and estimate the ultimate strength of macroscale SWNTs without binding agent.

Aproximación a las causas de la desigualdad económica

Resumen: El siguiente ensayo buscará presentar un enfoque alternativo al problema de la desigualdad económica. Abarcando cuestiones relacionadas con el análisis macroeconómico del desarrollo económico, la distribución de la renta entre trabajadores; la visión regional de la economía y las conexiones internacionales del desarrollo. Para ello, se establecieron como hipótesis el crecimiento económico y la inflación. Además, se analizará el impacto de un aumento en la PEA sobre la distribución del ingreso. Para terminar, el autor arribará a numerosas conclusiones, entre ellas, la dicotomía entre corto y largo plazo para la inflación, el aumento de la desigualdad ante el aumento del PBI en países subdesarrollados, y la relación inversa entre la PEA y el Índice de Gini.

Anistia política : conflito e conciliação no âmbito do Congresso Nacional Brasileiro (1964-1979)

Investiga a atuação do Congresso Nacional em torno da anistia política aos apenados pelo regime militar brasileiro. Acompanha o encaminhamento dado à referida questão através dos mecanismos formais de participação política, partindo-se mais especificamente das propostas legislativas apresentadas na Câmara dos Deputados e no Senado Federal. O recorte temporal adotado abarca o período compreendido entre o início do governo Castelo Branco (1964-1967), quando verificaram-se as primeiras articulações parlamentares em torno do tema, e a promulgação da Lei 6.683, em agosto de 1979. Situando-se o debate sobre a anistia na esfera da resistência democrática, são analisadas as estratégias empregadas pelos atores envolvidos, suas motivações, bem como os significados das disputas em torno daquela bandeira para os rumos do processo político.

PICES Press, Vol. 6, No. 2, July 1998

CREAMS, PICES and the exploration of the Japan/East Sea The state of the eastern North Pacific from September 97 to February 98 The state of the western North Pacific in the second half of 1997 The status of the Bering Sea in the second half of 1997 Hyung Tack Huh Report on GOOS Living Marine Resource Panel Meeting Global connections: A report of the GLOBEC International Open Science Meeting Update on U.S. GLOBEC research projects and coordination activities in the Northeast Pacific Institutional framework for oceanographic research in Japan The Kuroshio Edge Exchange Processes (KEEP) Project Report on NPAFC Workshop on Climate Change and Salmon Production A new ocean time series station in the western subarctic Pacific

Sobre el ecosistema de las aguas litorales de Mar del Plata. Niveles tróficos y cadenas alimentarias pelágicos demersales y bentónicos-demersales

The present paper deals with the trophic relationships of the communities of the coastal fishing area of Mar del Plata (Argentine). Different trophic levels of two main food chains (pelagic-demersal and benthic-demersal)were established. There are connections between both chains through certain species of invertebrates and fishes. This first try to establish the trophic relationships of our most important littoral communities, aims to set the preliminary bases for future energetic flow studies through the trophic web that gives a real economic importance to this productive area. (Document contains 45 pages)

Interações entre o Legislativo e o Executivo federal do Brasil na definição de políticas de interesse amplo: uma abordagem sistêmica, com aplicação na saúde

Aborda a relação entre o Legislativo e o Executivo na produção de políticas. Identifica os elementos do sistema de produção legislativa do Brasil (regras estruturantes, atores, recursos, instâncias de decisão e tipos de políticas produzidas) e propõe um modelo para o caso brasileiro de presidencialismo de coalizão, com base em estudos sobre a relação entre o presidente e o Congresso dos EUA e também na vasta produção existente sobre o contexto nacional. O sistema é estruturado pelo marco normativo de maior hierarquia, a Constituição, determinado historicamente, o qual privilegia a governabilidade com "accountability" e também orienta políticas segundo princípios de equidade, mas com responsabilidade orçamentária. O modelo considera que as agendas estratégicas dos atores são produto de variadas influências, incluindo o ¿status quo¿ (políticas existentes) e as demandas provenientes das conexões normativa e eleitoral. A partir desse modelo, o estudo analisa seus elementos e relações, aplicando-o a um conjunto abrangente de propostas legislativas (cerca de 21 mil proposições sobre todos os temas, apresentadas no Congresso entre 1999 e 2006, nas três vias).

Algorithms for colour image processing based on neurological models

373 p. : il., gráf., fot., tablas

El clasicismo en el País Vasco: Ramón de Basterra

[ES] Este trabajo analiza la obra del poeta y ensayista Ramón de Basterra (1888-1928), tratando de establecer las peculiaridades de su dimensión clasicista, en comparación con las líneas generales del clasicismo de la época. El clasicismo es visto como un componente fundamental de las ideologías conservadoras y del fascismo en el siglo XX. Se destacan las relaciones del autor con la intelectualidad vasca, especialmente bilbaína , y española. El conjunto de su obra, en particular La obra de Trajano (1921) y Las ubres luminosas (1923), representan una singular aportación a la historia del clasicismo, poco estudiado todavía en nuestro país. Para Basterra. La romanidad, la latinidad y la cristiandad como ejes de la misión civilizatoria de Occidente y su concepción esencialista y ahistórica de la raza y la nacionalidad españolas son elementos centrales de la obra basterriana. En la perspectiva de un análisis del fascismo que destaca el papel nuclear del culto a la romanidad, este autor podría ser definido como "protofascista".

A scientific forum on the Gulf of Mexico: The Islands in the Stream Concept. Proceedings of the Forum: 23 January 2008, Keating Education Center, Mote Marine Laboratory, Sarasota, Florida

The Scientific Forum on the Gulf of Mexico: The Islands in the Stream Concept took place in January 2008 in Sarasota, Florida. The purpose of the meeting was to bring together scientists and managers from around the Gulf of Mexico to discuss a range of topics on our knowledge of the Gulf of Mexico, from its geology to larger-scale connectivity to the Caribbean region, and their applications to the concept of a more integrated approach to area-based management. The forum included six panels of invited experts who spoke on the oceanographic and biological features in the Gulf of Mexico, including connections with Mexico and the Mesoamerican barrier reef system, and the legal and regulatory structure currently in place. The charge to the group was to share information, identify gaps in our knowledge, identify additional potential areas for protection, and discuss available science about connectivity and the potential value of establishing a marine protected area network in the Gulf of Mexico. (PDF has 108 pages.)

Resilient internet connectivity at Myerscough College

Myerscough College, a land-based further and higher education college in the north west, is one of the approximately 160 further education colleges in England to take additional connections to Jisc’s Janet network. Ian Brown, director of IT and MIS at the college, talks to us about why they’ve taken an extra four connections.

Self-Help Groups

“‘Self-Help Groups’ are … “... A way to start working that helps to build up the social connections which people find useful in support of their livelihoods objectives” “... Helping people to agree things and to speak together, giving people a stronger voice in decision-making and in negotiating with more powerful forces” “... A way of increasing the effectiveness of local actions” “... Providing easier access to micro-credit and other resources and services” (Pdf contains 4 pages).

Unilateral vs. Bilateral link-formation: Bridging the gap

We provide a model that bridges the gap between two benchmark models of strategic network formation: Jackson and Wolinsky' s model based on bilateral formation of links, and Bala and Goyal's two-way fl ow model, where links can be unilaterally formed. In the model introduced and studied here a link can be created unilaterally. When it is only supported by one of the two players the fl ow through the link suffers a certain decay, but when it is supported by both the fl ow runs without friction. When the decay in links supported by only one player is maximal (i.e. there is no flow) we have Jackson and Wolinsky 's connections model without decay, while when flow in such links is perfect we have Bala and Goyal' s two-way flow model. We study Nash, strict Nash and pairwise stability for the intermediate models. Efficiency and dynamics are also examined.