Stromal vascular fraction cell sheets angiogenic potential for tissue engineering and regenerative medicine applications

One of the biggest concerns in the Tissue Engineering field is the correct vascularization of engineered constructs. Strategies involving the use of endothelial cells are promising but adequate cell sourcing and neo-vessels stability are enduring challenges. In this work, we propose the hypoxic pre-conditioning of the stromal vascular fraction (SVF) of human adipose tissue to obtain highly angiogenic cell sheets (CS). For that, SVF was isolated after enzymatic dissociation of adipose tissue and cultured until CS formation in normoxic (pO2=21%) and hypoxic (pO2=5%) conditions for 5 and 8 days, in basal medium. Immunocytochemistry against CD31 and CD146 revealed the presence of highly branched capillary-like structures, which were far more complex for hypoxia. ELISA quantification showed increased VEGF and TIMP-1 secretion in hypoxia for 8 days of culture. In a Matrigel assay, the formation of capillary-like structures by endothelial cells was more prominent when cultured in conditioned medium recovered from the cultures in hypoxia. The same conditioned medium increased the migration of adipose stromal cells in a scratch assay, when compared with the medium from normoxia. Histological analysis after implantation of 8 days normoxic- and hypoxic-conditioned SVF CS in a hindlimb ischemia murine model showed improved formation of neo-blood vessels. Furthermore, Laser Doppler results demonstrated that the blood perfusion of the injured limb after 30 days was enhanced for the hypoxic CS group. Overall, these results suggest that SVF CS created under hypoxia can be used as functional vascularization units for tissue engineering and regenerative medicine.

Avaliação do desempenho de carteiras de fundos verdes em diferentes estados de mercado

Dissertação de mestrado em Finanças

A resolução de problemas como estratégia de promoção da educação ambiental para a sustentabilidade: uma intervenção com alunos de 11º ano centrada no tópico da exploração de recursos geológicos na disciplina de Biologia e Geologia

Relatório de estágio de mestrado em Ensino de Biologia e de Geologia no 3º Ciclo do Ensino Básico e no Ensino Secundário

Aprender a debater: o debate como atividade de interação e de participação democrática

Relatório de estágio de mestrado em Ensino de Inglês e de Espanhol no 3º Ciclo do Ensino Básico e no Ensino Secundário

A aprendizagem baseada na resolução de problemas na aprendizagem da Geologia: uma intervenção centrada no tópico "Ocupação antrópica e problemas de ordenamento"

Dissertação de mestrado em Ensino de Biologia e Geologia no 3º Ciclo do Ensino Básico e no Ensino Secundário

Programa de atividade física e saúde - Projeto M&M: análise da intervenção relativamente ao IMC e aptidão cardiorrespiratória

Relatório de estágio de mestrado em Ensino de Educação Física nos Ensinos Básico e Secundário

Relação entre aptidão física e sucesso académico: em alunos de final de ciclo: 8º e 9º ano de escolaridade

Relatório de estágio de mestrado em Ensino de Educação Física nos Ensinos Básico e Secundário

Programa de atividade física e saúde - Projeto M&M: análise da intervenção relativamente ao perímetro de cintura e aptidão cardiorrespiratória

Relatório de estágio de mestrado em Ensino de Educação Física nos Ensinos Básico e Secundário

Exploring the use of HPFRC and GFRP grids for the production of manhole covers

Corrosion of the steel reinforced concrete elements is one of the common pathologies that limits the long-term performance of urban infrastructures. This problem causes the loss of structural serviceability by decreasing the concrete-steel bond strength and reducing the cross section of the reinforcements. The present study introduces a new system for developing free-corrosion resistance prefabricated manhole covers for applications in the aggressive environments, i.e. wastewater collector systems, sewer systems, stormwater systems, etc. Fibre reinforced cement composites were applied in this system in order to suppress the corrodible steel mesh and maintain the structural ductility as well. Application of fibre reinforced polymer (FRP) system is adopted as the additional solution for increasing the load carrying capacity of these elements without concerns about corrosion. The effectiveness of the applied strategy in developing the manhole covers in terms of load carrying capacity and failure mode is evaluated in this research. Furthermore, this paper discusses a FEM-based simulation, aiming to address the possibility of calibrating the constitutive model parameters related to fracture modes I and II.

Occupational Ergonomics and Safety, Part 1

[Excerpt] It seems that significant progress has been made in last years in what concerns the scientific knowledge about work and its impact on workers’ wellbeing and health, which includes but it is not limited to occupational ergonomics and safety. Even considering that this domain is very transversal and comprehensive, as it may include researchers from multidisciplinary teams, research in this domain has clearly increased and we have now a larger number of scientific events, a higher number of researchers publishing their works, and a high emergence rate of research groups at universities and other research institutions, which was also followed by an increasing concern about these issues by governments and other regulators. Even though it is strikingly difficult to demonstrate it in an unequivocal way, this appears to have had a clear effect on companies and on their ability to implement plans and measures to prevent and control occupational risk factors. But despite these advances, occupational risk prevention is still a domain for which solutions are neither complete nor permanent, since the evolution of work systems gives rise to new challenges.

Comparative environmental life-cycle analysis of concretes using biomass and coal fly ashes as partial cement replacement material

Nowadays, the concrete production sector is challenged by attempts to minimize the usage of raw materials and energy consumption, as well as by environmental concerns. Therefore, it is necessary to choose better options, e.g. new technologies or materials with improved life-cycle performance. One solution for using resources in an efficient manner is to close the materials' loop through the recycling of materials that result either from the end-of-life of products or from being the by-product of an industrial process. It is well known that the production of Portland cement, one of the materials most used in the construction sector, has a significant contribution to the environmental impacts, mainly related with carbon dioxide emission. Therefore, the study and utilization of by-products or wastes usable as cement replacement in concrete can supply more sustainable options, provided that these type of concrete produced has same durability and equivalent quality properties as standard concrete. This work studied the environmental benefits of incorporating different percentages of two types of fly ashes that can be used in concrete as cement replacement. These ashes are waste products of power and heat production sectors using coal or biomass as fuels. The results showed that both ashes provide a benefit for the concrete production both in terms of environmental impact minimization and a better environmental performance through an increase in cement replacement. It is possible to verify that the incorporation of fly ashes is a sustainable option for cement substitution and a possible path to improve the environmental performance of the concrete industry.

Biofunctionalization of titanium surfaces for dental implants: osteogenic, anti-microbial and tribocorrosion resistant surfaces

PhD thesis in Biomedical Engineering

Detection of dangerous situations using a smart internet of things system

The Internet of Things (IoT) is a concept that can foster the emergence of innovative applications. In order to minimize parents’s concerns about their children’s safety, this paper presents the design of a smart Internet of Things system for identifying dangerous situations. The system will be based on real time collection and analysis of physiological signals monitored by non-invasive and non-intrusive sensors, Frequency IDentification (RFID) tags and a Global Positioning System (GPS) to determine when a child is in danger. The assumption of a state of danger is made taking into account the validation of a certain number of biometric reactions to some specific situations and according to a self-learning algorithm developed for this architecture. The results of the analysis of data collected and the location of the child will be able in real time to child’s care holders in a web application.

Princípios para a reabilitação sustentável low cost de edifícios antigos: estudo de um quarteirão no centro da cidade do Porto

Dissertação de mestrado em Construção e Reabilitação Sustentável

Potencialidades de elementos pré-fabricados de madeira na reabilitação

Dissertação de mestrado em Construção e Reabilitação Sustentáveis