978 resultados para Carne de bovino - maturação


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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

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Pós-graduação em Medicina Veterinária - FMVZ

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Pós-graduação em Medicina Veterinária - FMVZ

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Pós-graduação em Ciência Animal - FMVA

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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To evaluate the biochemical profile and protein concentration of whey from milk samples of healthy Murrah primiparous and pluriparous buffaloes, 30 female buffaloes were analyzed during a complete lactation. The animals were divided into three groups: G1 = 10 primiparous buffaloes, G2 = 10 pluriparous buffaloes with 2-3 lactations and G3 = 10 pluriparous buffaloes with > 3 lactations. The lactation period was divided into: early stage (I: 1-3 months of lactation), intermediate stage (T: 4-6 months of lactation) and final stage (F: 7-9 months of lactation). Before milk sampling, physical examination of the mammary gland, strip cup test and California Mastitis Test (CMT) were performed. After mammary quarters asepsis, 20mL of milk were collected monthly from each mammary quarter, during a complete lactation, in sterilized plastic bottles without preservative, in order to perform microbiological isolation, biochemical profile and protein electrophoresis in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), and 30mL of milk from each mammary quarter were collect, in sterilized plastic bottles containing preservative bronopol to perform the somatic cell count (SCC). A total of 1,042 milk samples were collected from the experimental groups during lactation, of which 923 samples showed negative reaction to CMT and negative microbiological isolation and were selected to biochemical profile analysis and protein electrophoresis in SDS-PAGE. There were influence of parity order and stage of lactation in biochemical profile and protein concentration of healthy Murrah buffaloes'whey. Primiparous buffaloes (G1) showed higher gamma-glutamyltransferase (GGT: 2,346 U/L), alkaline phosphatase (ALP: 181 U/L), phosphorus (P; 56.6mg/dL), potassium (K; 32.0mg/dL) and alpha-lactalbumin (458mg/dL). Buffaloes with 2-3 lactations (G2) showed higher SCC (70,700 cells/mL) and higher concentrations of total protein (1.55g/dL), albumin (100mg/dL), magnesium (Mg; 8.80mg/dL), chlorides (Cl; 176mg/dL), iron (Fe; 10.7 mu g/dL), sodium (Na; 178mMol/L) and lactoferrin (59.5mg/dL). Bufalloes with > 3 lactations (G3) showed higher concentrations of total calcium (Ca; 41.8mg/dL), ionized calcium (iCa; 2.92mMol/L), immunoglobulin A (IgA; 1.32mg/dL), serum albumin (99.1mg/dL), immunoglobulin G (IgG; 49.7mg/dL) and beta-lactoglobulin (1,068mg/dL). During lactation it was observed increase in SCC, GGT, ALP, total protein, albumin, P, Mg, Cl, Na, lactoferrin, serum albumin, IgG and alpha-lactalbumin, as well as decrease in concentrations of Ca, Fe, iCa, K, IgA and beta-lactoglobulin in buffaloes'whey. The results may be used as reference for buffaloes and to support diagnosis and prognosis of diseases common to lactation periods.

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Pós-graduação em Agronomia (Ciência do Solo) - FCAV

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The development of the stomach of ruminants requires further studies because it is a complex process. Therefore, was analyzed the histology and histomorphometry of reticulum at each gestation period. Samples of Nelore fetuses (Bos taurus indicus) was divided in 5 groups: 1 - fetuses at 9 to 15 weeks (8 to 21cm) of gestation, 2 - fetuses at 16 to 22 weeks (23 to 37cm) 3 - fetuses at 23 to 29 weeks (40 to 58cm), 4 - fetuses at 30 to 36 weeks (61 to 77cm) and 5 - fetuses at 37-43 weeks (79 to 88cm). The histological sections were stained with hematoxylin and eosin and Mallory's trichrome and examined by light microscopy. In group 1, the fetuses with 11 cm, showed mucosal projections that eventually become primary crests, lamina propria and submucosa are fused and external and internal muscular and similar. At 16.5 inches, the primary crests had lamina propria and at 18.5 cm, appeared connections between the ridges. In group 2, at 31 cm started epithelial involvement around the crests. In group 3, the fetuses of 42 cm revealed the first secondary papillae and muscular mucosa in the upper portion of the primary crests. Thereafter, it was increased epithelial due to its positioning around the crests, thickening of the lamina propriasubmucosa, muscular layer, especially the internal muscular and serosa. It was concluded that the most visible changes occur in fetuses belonging to the first 3 groups, measurements for the epithelium, muscle layer and total wall were increasing and the other analyzed layers showed variations during fetal development.

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)