O virus do mixoma no coelho do mato (Sylvilagus minenses), sua transmissão pelos Aedes scapularis e aegypti

The brazilian wild rabbit (Sylvilagus minensis) is sensible to the virus of the mixomatosis but the desease takes on it a mild character, lasts for long time and generally do not kill the animal. The tumors are generally smaller and less numerous than those of the domestic rabbit, but sometimes there were noted large and flat lesions (fig. 3). The natural infection of the wild rabbit may be quite common not only because many rabbits caught in the country were found to be immune as also because it was found among the animals caught in the country near Rio, one that was infected with mixomatosis. The experimental infection of the Sylvilagus may be easily obtained by cutan, subcutan or conjuntival way and also when a health wild rabbit is placed in the same cage with a sick domestic animal. It is also possible to obtain the infection of the wild and domestic rabbits by the bite of infected blood sucking insects as fleas and mosquitoes. The infected mosquito can transmit the disease 2 or 3 times til 17 days after an infective meal on a sick rabbit. The transmission is a mecanical one and only the proboscis of the insect contains the virus as it was shown by the inoculation of emulsions of the proboscis, thorax and abdomen of the mosquito. Though mecanical this kind of transmission acts as an important epidemiological mean of dissemination of the deseasse and splains the suddendly outbreaks of mixomatosis in rabbits breedings where no new rabbits were introduced since very long time. The transmition of mixomatosis by fleas (Slenopsylla) was at first demonstrated by us, then S. Torres pointed out the capacity of Culex fatigans to transmit the desease and now we have proved that Aedes scapularis and Aedes aegypti were also able to transmit it (Foto 1 and 2). The virus of the mixomatosis (Chlamidozoon mixoma) is seen on the smeavs of the tumors of the wild reabbit with the same morphology, as in the material of the domestic animal.

Aspectos da fitofisionomia do Sul do Estado de Mato Grosso

The Brazilian Institute for Agrarian Reform is promoting researches on soil fertility in southern Mato Grosso, complemented by a survey of the main types of vegetation. The data obtained were also to be used to build up the legends for the map of vegetation. Photos were taken by the authors and botanical material collected in order to permit the identification of important species. Observations made led to the above table of main physiognomies.

El Autobús accesible: algo más que un piso bajo: conclusiones de la jornada

A lo largo de las intervenciones se presentaron distintos enfoques, datos e información sobre las condiciones de accesibilidad en el servicio de transporte urbano en autobús, particularmente en relación con los vehículos de piso bajo que lo prestan. Las presentaciones se centraron en las distintas soluciones habilitadas para atender las necesidades de movilidad de todas las personas, las características de los servicios de transporte ofrecidos y sus problemas técnicos y operativos, así como los retos que plantea la seguridad, la formación del personal, la gestión del sistema o el coste de la aplicación de las innovaciones desarrolladas.

What is paragonimus rudis (diesing, 1850)?: report on a field study in Mato Grosso, Brazil

Paragonimus rudis was found in the lungs of a giant otter Lutra (pteronura) brasiliensis by Natterer in 1828, who dissected the animal in the former capital Mato Grosso (=Vila Bela), Brazil. The flukes were described by Diesing in 1850, and redescribed by Braun in 1901. Both descriptions do not allow to identify the species. Therefore, P. rudis must be regarded a "nomen nudum". Because its rediscovery is desirable with regard to historical reasons and nomenclatoric questions, a field study was performed in Mato Grosso in 1980. Of 354 freshwater crabs from 24 localities collected and examined for parasitic infections, about 25% were found to be infected with 7kinds of trematode larvae, which differed distincly from Paragonimus-metacercariae. The question, whether P. rudis or other lung fluke species do not seem to occur or cannot be found any longer in the area investigated by us, is discussed.

Sarcophagid flies (Diptera) from sinop, state of Mato Grosso, Brazil

In Sinop, State of Mato Grosso, Brazil, for the first time specimens of Euboettcheria, with seven species, are dominant (54.39%) among the Sarcophagid flies. Two new species of Euboettcheria (alvarengai and roppai) and females of known species are described and figured. Notochaeta sinopi n.sp. and Sinopiella rufopilosa n. gen., n. sp. are also described.

Biomphalaria intermedia in Mato Grosso do Sul, Brazil, and Misiones, Argentina (Pulmonata: Planorbidae)

The occurrence of Biomphalaria intermedia (Paraense & Deslandes, 1962) is recorded at Três Lagoas and Dourados (state of Mato Grosso do Sul, Brazil), and Parque Nacional Iguazú (province of Misiones, Argentina), west and southwest, respectively, of its known western limit, in the state of São Paulo. The species was also found at Parapuã, a new locality to São Paulo state.

Liquid chromatography-tandem mass spectrometry (LC/APCI-MS/MS) methods for the quantification of captan and folpet phthalimide metabolites in human plasma and urine.

Captan and folpet are fungicides largely used in agriculture. They have similar chemical structures, except that folpet has an aromatic ring unlike captan. Their half-lives in blood are very short, given that they are readily broken down to tetrahydrophthalimide (THPI) and phthalimide (PI), respectively. Few authors measured these biomarkers in plasma or urine, and analysis was conducted either by gas chromatography coupled to mass spectrometry or liquid chromatography with UV detection. The objective of this study was thus to develop simple, sensitive and specific liquid chromatography-atmospheric pressure chemical ionization-tandem mass spectrometry (LC/APCI-MS/MS) methods to quantify both THPI and PI in human plasma and urine. Briefly, deuterated THPI was added as an internal standard and purification was performed by solid-phase extraction followed by LC/APCI-MS/MS analysis in negative ion mode for both compounds. Validation of the methods was conducted using spiked blank plasma and urine samples at concentrations ranging from 1 to 250 μg/L and 1 to 50 μg/L, respectively, along with samples of volunteers and workers exposed to captan or folpet. The methods showed a good linearity (R (2) > 0.99), recovery (on average 90% for THPI and 75% for PI), intra- and inter-day precision (RSD, <15%) and accuracy (<20%), and stability. The limit of detection was 0.58 μg/L in urine and 1.47 μg/L in plasma for THPI and 1.14 and 2.17 μg/L, respectively, for PI. The described methods proved to be accurate and suitable to determine the toxicokinetics of both metabolites in human plasma and urine.

Más allá de la ley de May: disparidades curvilíneas y conflicto intrapartidista. El caso de Cataluña

Este artículo pretende contribuir al debate sobre el papel que desempeñan los adherentes y activistas y, de modo especial, a intentar plantear de nuevo el problema de su influencia dentro del partido. Para ello se revisa el trabajo de May (1973), sus posteriores desarrollos empíricos, y se intenta comprobar su validez en los principales ejes que marcan la competencia política catalana. Una vez comprobada la existencia de disparidades ideológicas en el caso catalán, el papel se centra en discutir algunas de las limitaciones del modelo de May. Ello permite, a su vez, sugerir la necesidad de elaborar un nuevo modelo de conflicto partidista que tenga presente tanto las relaciones de poder verticales (entre dirigentes y activistas) como horizontales (entre los mismos dirigentes). Para mostrar empíricamente las debilidades señaladas en el modelo de May, el análisis termina con un caso estudio que intenta ilustrar la dificultad de asociar disparidades con conflicto interno.

Indirect hydrogen analysis by gas chromatography coupled to mass spectrometry (GC-MS).

Gas chromatography (GC) is an analytical tool very useful to investigate the composition of gaseous mixtures. The different gases are separated by specific columns but, if hydrogen (H2 ) is present in the sample, its detection can be performed by a thermal conductivity detector or a helium ionization detector. Indeed, coupled to GC, no other detector can perform this detection except the expensive atomic emission detector. Based on the detection and analysis of H2 isotopes by low-pressure chemical ionization mass spectrometry (MS), a new method for H2 detection by GC coupled to MS with an electron ionization ion source and a quadrupole analyser is presented. The presence of H2 in a gaseous mixture could easily be put in evidence by the monitoring of the molecular ion of the protonated carrier gas. Copyright © 2013 John Wiley & Sons, Ltd.