Evaluation of emergency vehicle signal preemption on the Route 7 Virginia corridor.

Federal Highway Administration, Office of Operations Research and Development, McLean, Va.

Studies in the role of the public school teacher,

Includes bibliographies.

How do I do this when I can't see what I'm doing? : information processing for the visually disabled /

Includes bibliographical references and index.

First tentative draft : instructions for field work of the signal branch of the engineering section of the Interstate Commerce Commission /

Cover title.

A study in the design of an arithmetic element for serial processing in a linear iterative structure /

Vita.

Comprehensive security in data processing systems : the state of the art /

"UIUCDCS-R-75-725"

The role of the MCDU in the parallel element processing ensemble (PEPE) /

Thesis (M. S.)--University of Illinois at Urbana-Champaign.

The Abelian equations of the tenth degree irreducible in a given domain of rationality,

Thesis (Ph. D.)--University of California, 1912.

A fishery imbroglio: treating of a question in the domain of intersexual politics; a sketch,

Mode of access: Internet.

Liberia; or, The early history & signal preservation of the American colony of free negroes on the coast of Africa.

Mode of access: Internet.

American signal book, or, The United States telegraph vocabulary; being an appendix to Elford's marine telegraph signal book ... adapted to the use of the new semaphoric telegraph : to which is added the Boston Harbor signal book /

Mode of access: Internet.

Impact of the computer on job analysis in the United States Air Force /

Photocopy.

High-resolution spatial processing in underwater acoustics /

Mode of access: Internet.

Rabbit CYP4B1 engineered for high-level expression in Escherichia coli: ligand stabilization and processing of the N-terminus and heme prosthetic group

Modifications at the N-terminus of the rabbit CYP4B1 gene resulted in expression levels in Escherichia coli of up to 660 nmol/L. Solubilization of the enzyme from bacterial membranes led to substantial conversion to cytochrome P420 unless alpha-naphthoflavone was added as a stabilizing ligand. Mass spectrometry analysis and Edman sequencing of purified enzyme preparations revealed differential N-terminal post-translational processing of the various constructs expressed. Notably, bacterial expression of CYP4B1 produced a holoenzyme with >98.5% of its heme prosthetic group covalently linked to the protein backbone. The near fully covalently linked hernoproteins exhibited similar rates and regioselectivities of lauric acid hydroxylation to that observed previously for the partially heme processed enzyme expressed in insect cells. These studies shed new light on the consequences of covalent heme processing in CYP4B1 and provide a facile system for future mechanistic and structural studies with the enzyme. (C) 2003 Elsevier Science (USA). All rights reserved.

Systematic characterization of the zinc-finger-containing proteins in the mouse transcriptome

Zinc-finger-containing proteins can be classified into evolutionary and functionally divergent protein families that share one or more domains in which a zinc ion is tetrahedrally coordinated by cysteines and histidines. The zinc finger domain defines one of the largest protein superfamilies in mammalian genomes; 46 different conserved zinc finger domains are listed in InterPro (http://www.ebi.ac.uk/InterPro). Zinc finger proteins can bind to DNA, RNA, other proteins, or lipids as a modular domain in combination with other conserved structures. Owing to this combinatorial diversity, different members of zinc finger superfamilies contribute to many distinct cellular processes, including transcriptional regulation, mRNA stability and processing, and protein turnover. Accordingly, mutations of zinc finger genes lead to aberrations in a broad spectrum of biological processes such as development, differentiation, apoptosis, and immunological responses. This study provides the first comprehensive classification of zinc finger proteins in a mammalian transcriptome. Specific detailed analysis of the SP/Kruppel-like factors and the E3 ubiquitin-ligase RING-H2 families illustrates the importance of such an analysis for a more comprehensive functional classification of large protein families. We describe the characterization of a new family of C2H2 zinc-finger-containing proteins and a new conserved domain characteristic of this family, the identification and characterization of Sp8, a new member of the Sp family of transcriptional regulators, and the identification of five new RING-H2 proteins.