941 resultados para low level laser therapy
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In this study, the background activity of beta-glucuronidase (GUS) was analyzed histochemically and fluorometrically in the negative control of Laminaria japonica (Phaeophyta) thalli, showing low level of activity. GUS gene transformation without selectable gene in L. japonica was performed using four different promoters, i.e., Cauliflower mosaic virus 35S promoter (CaMV35S) from cauliflower mosaic virus, ubiquitin promoter (UBI) from maize, adenine-methyl transfer enzyme gene promoter (AMT) from virus in green alga Chlorella, and fucoxanthin chlorophyll a/c-binding protein gene promoter (FCP) from diatom Phaeodactylum tricornutum. The GUS transient activity was determined fluorometrically after bombarding sliced parthenogenetic sporophytes explants, and it was found that the activity resulting from CaMV35S and FCP promoters (in 114.3 and 80.6 pmol MU min(-1) (mg protein)(-1), respectively) was higher than for the other two promoters. The female gametophytes were bombarded and regenerated parthenogenetic sporophytes. FCP was the only promoter that resulted in detectable GUS chimeric expression activity during histochemical staining and polymerase chain reaction. Results of Southern blot showed that GUS gene was integrated with the L. japonica genome.
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The color mutations in Bangiaceae were investigated by treating the blades, conchocelis and conchospores phase of Bangia sp., Porphyra yezoensis, and P. haitanensis sampled in China with mutagen N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). A high percentage of mutation in different expression characteristics in all three phases were shown within optimum mutagen concentrations. Among mutagenized blades, mutations occurred on single cells, which is a direct outcome of mutation of haploid cells. The mutation of mutagenized conchocelis resulted in a two-step process: low-level expression in conchocelis phase, and high-level expression in progeny, explaining that mutation took place in diploid cells. The mutations of conchospores were expressed immediately at germination of spores, indicating a change in ploidy. This paper reports the process of meiosis and its effect on frond development, and the relation between color mutations and morphological characteristics expressed by mutations in Bangiaceae.
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本文主要运用稳定加液-反应系统对海水中方解石和文石形成时稀土元素的共沉淀现象进行了分析,研究了稀土元素在固-液体系中的迁移、转化和分配。进而在对其定量描述的前提下,研究了稀土元素共沉淀对各种反应条件的响应,并对共沉淀行为的机制进行了探讨。 本实验首先运用pH测试、高精度滴定分析等手段测定了实验中的一些基本参数,如[H+]、碱度和[Ca2+],根据计算结果获得了各碳酸体系要素,并以此为基础建立了5℃、15℃和25℃及pCO2=0.003atm下海水中方解石或文石的沉淀动力学方程。实验结果表明: 1)在各条件下,方解石或文石的沉淀速率(R)和其在海水中过饱和度(Ω)存在很好的线性相关性,即海相碳酸盐的沉淀动力学方程可以通过下面的基本表达式来表示:LogR=k*Log(Ω-1)+b ; 2)过高的稀土元素浓度会对文石或方解石的沉淀产生抑制作用,进而对共沉淀过程中YREEs的分异和分馏产生一定的影响。相比方解石而言,文石的沉淀动力学过程承受稀土元素的干扰能力更强; 3)不同温度下得到的方解石或文石各自的沉淀动力学方程存在明显的差异,表明这一过程受热力学因素控制。相对于方解石而言,温度对文石的沉淀动力学的影响更为显著。 与前人研究不同的是,本实验中YREEs的浓度设定在非常低的范围内,从而避免了过高浓度YREEs对方解石或文石沉淀动力学过程的干扰。在最终的反应液中,各种实验条件非常接近自然环境。有关稀土元素的共沉淀行为主要得出以下定性或定量化结论: 1)YREEs在随方解石或文石的共沉淀过程中,均发生了强烈的分异作用。在方解石实验中,稀土元素的分异系数分布曲线呈凸状分布;而在文石实验中,稀土元素的分异系数随原子序数的增加逐渐减小,遵循镧系收缩的规律。总的来说,稀土元素,尤其轻稀土元素在文石中的分异作用要强于方解石。 2)无论是方解石还是文石,沉淀速率对YREEs的分异作用都有着明显的影响。在方解石中,YREEs的分异系数随沉淀速率的增加呈一致性递减趋势;而在文石中,其分异系数对文石沉淀速率有着截然不同的响应:轻稀土元素(La, Ce, Nd, Sm, Eu, Gd)的分异系数随文石沉淀速率的增加而下降,而重稀土元素(Ho, Y, Tm, Yb , Lu)的分异系数则随文石沉淀速率的增加呈上升趋势。 3)在方解石中YREEs的分异系数之间存在非常好的相互关系,表明这些元素是以成比例的方式参与共沉淀。整个谱系呈现中等强度的分馏,MREE相对于LREE和HREE要更为富集;在文石中由于沉淀速率的作用不同,只有Y、Ho、Yb、Lu等元素的分异系数之间有较好的相互关系。YREEs出现了差异性的强烈分馏,在新生成沉淀中轻稀土元素相对于重稀土元素强烈富集。 4)YREEs在溶液中和碳酸盐晶体表面的碳酸根配位形式对YREEs在共沉淀过程中的分异作用极为重要,YREEs在碳酸盐晶体表面的吸附是整个谱系发生分馏效应的关键环节。对于文石来讲,晶体中有效YREE离子和Ca离子半价大小之间的相近程度是其分馏效应的关键因素;而对于方解石来说,YREEs在方解石晶格中的安置就是其分馏效应的关键控制因子,但在晶格安置中起到关键作用的是YREEs和方解石中O原子之间离子键M-O的键长,而非离子半径。 5)综合YREEs在方解石中的分异作用和分馏效应,我们认为M2(CO3)3-CaCO3和MNa(CO3)2-CaCO3是最为可能的两种固体溶液形成模式。 最为重要的是,对比我们的实验结果与前人在灰岩、叠层石、微生物成因碳酸盐等方解石质载体中的研究成果,两者之间出现了非常好的一致性。我们认为方解石质载体将是重建古海水中稀土元素相关信息的重要工具。相比之下,文石质载体不适合作为类似的载体。
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The vertical distribution and stage-specific abundance of Calanus sinicus were investigated on three key transects in the southern Yellow Sea and the northern East China Sea in August 1999. The results showed that in summer C. sinicus shrank its distribution area to the central cold (less than or equal to10degreesC) bottom water in the Yellow Sea, i.e. the Yellow Sea Cold Bottom Water, remaining in high abundance (345.7 ind m(-3)). In the northern East China Sea on a transect from the mouth of the Yangtze River to the Okinawa trench, only a few individuals appeared in the inner side and none had been found either in the upper layer or in the deep layer of the outer shelf area. The population of C. sinicus in YSCBW consisted of mainly adults (46.83%) and C5 (37.41%). C1-C4 only accounted for 15.76%. The low proportion of the earlier copepodite stages and the high female:male ratio (11.39) indicated that the reproduction of C. sinicus in YSCBW was at a very low level due to the low temperature and low food concentration. It is concluded that the dramatic decrease of C. sinicus population in the shelf area of China seas in summer is caused by the shrinkage of its distribution area and the YSCBW served as an oversummering site.
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Due to the influence of human activities, nutrient concentrations, nutrient ratios and phytoplankton composition have notably changed in Jiaozhou Bay, China since the 1960s. From the 1960s to the 1990s, nutrient concentrations have increased 1.4 times for PO4-P, 4.3 times for NO3-N, 4.1 times for NH4-N and 3.9 times for DIN. The atomic ratio of DIN:PO4-P increased very rapidly from 15.9 +/- 6.3 for the 1960s, to 37.8 +/- 22.9 for the 1990s. SiO3-Si concentration has remained at a very low level from the 1980s to the 1990s. The high ratio of DIN: PO4-P and low ratios of SiO3-Si:PO4-P (7.6 +/- 8.9) and SiO3-Si:DIN (0.19 +/- 0.15) showed the nutrient structure of Jiaozhou Bay has changed from more balanced to unbalanced during the last 40 years. The possibility that DIN and/or PO4-P as limiting factors of Jiaozhou Bay phytoplankton has been lessened or eliminated and that of SiO3-Si limiting has been increased. The changes in nutrient structure may have led to the decrease of large diatoms and a shift of phytoplankton species composition. It is likely that there is a trend from large diatoms to smaller cells in Jiaozhou Bay. (C) 2001 Academic Press.
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Random amplified polymorphic DNA ( RAPD) markers were used to measure genetic diversity of Coelonema draboides ( Brassicaceae), a genus endemic to the Qilian Mountains of the Qinghai-Tibet Plateau. We sampled 90 individuals in 30 populations of Coelonema draboides from Datong and Huzhu counties of Qinghai Province in P. R. China. A total of 186 amplified bands were scored from the 14 RAPD primers, with a mean of 13.3 amplified bands per primer, and 87% ( 161 bands) polymorphic bands (PPB) was found. Analysis of molecular variance (AMOVA) shows that a large proportion of genetic variation (84.2%) resides among individuals within populations, while only 15.8% resides among populations. The species shows higher genetic diversity between individuals than other endemic and endangered plants. The RAPDs provide a useful tool for assessing genetic diversity of rare, endemic species and for resolving relationships among populations. The results show that the genetic diversity of this species is high, possibly allowing it to adapt more easily to environmental variations. The main factor responsible for the high level of differentiation within populations and the low level of diversity among populations is probably the outcrossing and long-lived nature of this species. Some long-distance dispersal, even among far separated populations, is also a crucial determinant for the pattern of genetic variation in the species. This distributive pattern of genetic variation of C. draboides populations provides important baseline data for conservation and collection strategies for the species. It is suggested that only populations in different habitats should be studied and protected, not all populations, so as to retain as much genetic diversity as possible.
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设计了一种基于多微处理器的智能超声探测系统。该系统采用由上位工作模式控制单元和下位智能超声传感器阵列组成的分布式结构。每个超声传感器均由独立的微处理器控制,并完成实时数据处理、抗干扰处理、故障报警以及数据通信等功能。上位控制单元根据移动机器人的运动状态采用不同的控制策略,使下位传感器阵列分组并行工作,提高了探测系统的实时性;下位传感器采用"阈值比较法"、"改进型递推均值滤波"算法和模糊信息处理技术,提高了探测系统的准确性、可靠性。将该探测系统安装于RIRA-II移动机器人上,进行了基于主动视觉和超声信息的运动目标跟踪实验,实验表明超声探测系统运行可靠、稳定。
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Now low porosity and low permeability reservoir is one of the main targets of exploration for the onshore oilfields of China. Most of the reservoirs are none flowing because of bad formation percolation condition, poor gas oil ratio , low formation pressure coefficient and other factors. In the recent years, a number of domestic oilfields have carried out some research work and achieved some success on oil testing and production technology in such formation. But by now, there is still no systematic and mature technology, particularly testing technology in none flowing formation is still needed further study. Based on study the key problem of well testing and interpretation technology in none flowing formation, solve the important problems in well testing technology, continuously improve and innovate geological information acquisition technology for none flowing reservoir, accurately acquire boundary information and evaluate reservoir flow characteristics. Its wide application remarkable result has shown. The main results and cognitions obtained from research are as follows: 1. This new technology research results help solve the occurrent problems in well testing process for none flowing formations, such as small investigation radius, poor representative of interpretation results from the poor data, low level application of interpretation results. This new technology helps create favorable conditions for early precise reservoir evaluation and reduction of the risk of exploration. 2. The technological difficulties for none flowing well testing are successfully solved by using none flowing formation combined mechanical tool string .This method has been proved by its applications to be able to improve the efficiency of the testing and the quantity of the acquired test data ,and so as to enhance the application of the interpretation results of the test in development of oil fields. 3. The application of the rotary formation tester, selective test valve, well testing string and their allier tools help to resolve problems such as the operation of opening and shutting-in the well under different well conditions, to broaden the scope of well test technology for none flowing formations. 4. Refined Testing Technique for production Wells has greatly shortened the testing dwration and improved the efficiency and accuracy of operation, enriched test results, and at the same time created conditions for conducting multi-well interference well testing.
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The surface of the Earth is continuously undergoing changes as a result of weathering-erosion, plate tectonics and volcanic processes. Continental weathering-erosion with its complex rock-water interactions is the central process of global biochemical cycling of elements, and affects the long-term ocean atmosphere budget of carbon dioxide both through the consumption of carbonic acid during silicate weathering and through changes in the weathering and burial rates of organic carbon. Rates of the weathering-erosion depend on a variety of factors, in particular rock properties and chemical composition, climate (especially rainfall), structure, and elevation. They are quite variable on a regional scale. Thus, environmental changes in a region could be indicated by the history of weathering-erosion in the region. Recent attention has focused on increased silicate weathering of tectonically uplifted areas in the India-Asia collision zone as a possible cause for falling atmospheric CO_2 levels in the Cenozoic era. The wind blown dust deposits in the Loess Plateau is derived from the arid and semiarid regions in northwestern China, in turn, where the deposits have been derived from the Qinghai-Xizang Plateau and the high mountains around. Therefore, geochemistry of the wind blown loess-paleosol and red clay sequences may provide insight both to paleoenvironmental changes on the Loess Plateau, and to the uplift and weathering-erosion histories of the Qinghai-Xizang Plateau. In this paper, uranium-thorium series nuclides and cosmogenic ~(10)Be have been employed as tracers of weathering intensities and histories of the dust sediments in the Loess Plateau. Major elements, such as Na, Al, Fe etc., are also used to estimate degree of chemical alteration of the dust sediments and to rebuild the history of weathering on the Loess Plateau. First of all, using a low-level HPGe γ-ray detector, we measured U and Th series nuclides in 170 loess and paleosol samples from five sites in the Loess Plateau, going back 2.6 Ma. The results show that ~(238)U activities are disequilibrium with its daughter nuclide ~(230)Th in young loess-paleosol sequence, indicating that weathering was happened both in dust deposition site and in dust source regions. Using concentrations of ~(238)U and ~(232)Th in the samples, we estimated the amounts of ~(238)U leached out of from paleosols due to weathering. Further, based on analyses of ~(230)Th in paleosols deposited in the past ca. 140 ka, we determined when the paleosols weathered in the source regions. We conclude that most of the weathering in the dust-source regions may have occurred during the interglacials before dust deposition.
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Construal Level Theory is a kind of social cognitive theory, with the main conclusion of that the more time distance is, the more people use high level construal and the more high level construal affect in judging, decision making and such of these cognitive processes, and the low level construal has the mirror process as well. In these ten years, construal level theory is developing very fast and some of the researches of consumer behavior has concerned this developed trend very much. In 2007’s Journal of Consumer psychology, half of the papers focused on construal topics and ask for more researches about these topics. So our research mainly focused on such topics, the application of construal level theory in consumer behavior research. The first part is contained 3 experiments which mean to discuss the questions from reviewing the prior literatures. The research methods, manipulated definition, construal level factors VS construal level events are the goal of first part of this study. The results showed that construal level’s effect might be a consecutive variable and has a consecutive trend when time goes by. Time distance might not be a semantic cognitive effect to construal level but a internal ones for when there’s no verbal information of time distance the results would have the same one as well. The second part of the study is focus on a simplified model of decision-making, which contained two times decision-making, to detect whether and how would the prior decision making affect the next decision making. The study conducted the time distance in first decision making with the situation for the decision and conducted the second time distance in the time distance between the two decision situations. Although we couldn’t get the detail of the weight changes in construal level factors limited by our research, we can have a logical hypothesis based on “one-down- the other-up”. The results showed that the level of construal factors can affect the memory and the more the factors affected, the bigger the error would be. The first time distance and second time distance could effect the second decision in a interaction-way, and support the hypothesis. The whole research is mean to explore the application of construal level theory in consumer behavior research. The conclusion suggests that the periods of products and the consuming may need different marketing strategies in a long-term perspective and time distance should be control are detected in marketing analysis even there’s no time information on the paper.
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Color has an unresolved role in the rapid process of natural scene. The temporal changes of the color effect might partly account for the debates. Besides, the distinction of localized and unlocalized information has not been addressed directly in these color studies. Here we present two experiments that investigate whether color contributes to categorization in a briefly flashed natural image and also whether it is mediated by time and low-level information. By controlling the interval between target and mask stimuli, Experiment 1 tested the hypothesis that colors could facilitate in the early stage of scene perception and the effect would decay in later processing. Experiment 2 examined how the randomization of local phase information influenced the color’s advantage over gray. Together, the results suggest that color does enhance natural scene categorization at short exposure time. Furthermore, results imply that effect of color is stable between 12 and120ms, and is not accounted by showing the structures organized by localized information. Therefore,we concluded that color always make effect in the process of rapid scene categorization, and do not depend on localized information. Thus, the present study is an attempt to fill the gap in previous research; its results is an contribution to deeper understanding of the role of color in natural scene perception.
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Since the middle of 1980's, the mechanisms of transfer of training between cognitive subskills rest on the same body of declarative knowledge has been highly concerned. The dominant theory is theory of common element (Singley & Anderson, 1989) which predict that there will be little or no transfer between subskills within the same domain when knowledge is used in different ways, even though the subskills might rest on a common body of declarative knowledge. This idea is termed as "principle of use specificity of knowledge" (Anderson, 1987). Although this principle has gained some empirical evidence from different domains such as elementary geometry (Neves & Anderson, 1981) and computer programming (McKendree & Anderson, 1987), it is challenged by some research (Pennington et al., 1991; 1995) in which substantially larger amounts of transfer of training was found between substills that rest on a shared declarative knowledge but share little procedures (production rules). Pennington et al. (1995) provided evidence that this larger amounts of transfer are due to the elaboration of declarative knowledge. Our research provide a test of these two different explanation, by considering transfer between two subskills within the domain of elementary geometry and elementary algebra respectively, and the inference of learning method ("learning from examples" and "learning from declarative-text") and subject ability (high, middle, low) on the amounts of transfer. Within the domain of elementary geometry, the two subskills of generating proofs" (GP) and "explaining proofs" (EP) which are rest on the declarative knowledge of "theorems on the characters of parallelogram" share little procedures. Within the domain of elementary algebra, the two subskills of "calculation" (C) and "simplification" (S) which are rest on the declarative knowledge of "multiplication of radical" share some more procedures. The results demonstrate that: 1. Within the domain of elementary geometry, although little transfer was found between the two subskills of GP and EP within the total subjects, different results occurred when considering the factor of subject's ability. Within the high level subjects, significant positive transfer was found from EP to GP, while little transfer was found on the opposite direction (i. e. from GP to EP). Within the low level subjects, significant positive transfer was found from EP to GP, while significant negative transfer was found on the opposite direction. For the middle level subject, little transfer was found between the two subskills. 2. Within the domain of elementary algebra, significant positive transfer was found from S to C, while significant negative transfer was found on the opposite direction (i. e. from C to S), when considering the total subjects. The same pattern of transfer occurred within the middle level subjects and low level subject. Within the high level subjects, no transfer was found between the two subskills. 3. Within theses two domains, different learning methods yield little influence on transfer of training between subskills. Apparently, these results can not be attributed to either common procedures or elaboration of declarative knowledge. A kind of synthetic inspection is essential to construct a reasonable explanation of these results which should take into account the following three elements: (1) relations between the procedures of subskills; (2) elaboration of declarative knowledge; (3) elaboration of procedural knowledge. 排Excluding the factor of subject, transfer of training between subskills can be predicted and explained by analyzing the relations between the procedures of two subskills. However, when considering some certain subjects, the explanation of transfer of training between subskills must include subjects' elaboration of declarative knowledge and procedural knowledge, especially the influence of the elaboration on performing the other subskill. The fact that different learning methods yield little influence on transfer of training between subskills can be explained by the fact that these two methods did not effect the level of declarative knowledge. Protocol analysis provided evidence to support these hypothesis. From this research, we conclude that in order to expound the mechanisms of transfer of training between cognitive subskills rest on the same body of declarative knowledge, three elements must be considered synthetically which include: (1) relations between the procedures of subskills; (2) elaboration of declarative knowledge; (3) elaboration of procedural knowledge.
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In this study, bibliometric method was usded in the investigation of 2274 papers concerning child developmental and educational psychology, which were published during the ten years of 1979-1988, in 14 psychological journals and 97 other scientific journals. According to the quantitative and qualitative analyses, the results are as follows: 1979-1988 saw the rapid development and prosperous period in China's child developmental and educational psychology, During which more papers were published and more fields couched than in the psvious thirty years. The number of literature publications increased and went to the peak in 1983 and 1984, and came down since 1985. The trend was found to result from the decrease in popular science introductions of psychology, which reflected that a heat of psychology had appeared in 1983 and started to cool in 1985. At the mean time, the number of research reports had been holding a steady increase by 1987 and decreased obviously in 1988, especially in the fields of cognitive and social development. There could be several possible explanations of this phenonemon: Piagetian studies are becoming fewer and the eakening of Piaget's influence might predict a period of standstill in the field of developmental psychology in China; As researches become more and more difficult, researchers have turned to be more cautious in lay out their reports; the cutdown of fees and staff could also be one of reasons for less publication in 1988. As the factors mentioned above still exist and their influences last, the number of papers are not expected to increase in the near future. The field of thinking and menory is closely connected with that of artificial intelligence. The downhill situations in these two fileds should be taken seriously. 2. The types of research work are divided on the bases of their problem raising. The trends show that the deepening studies, which represent a comaratively higher level of exploration, are waving fewer, while repeated studies and creative studies are becoming more as the years go along. This fact is worth being further analysed. Big progress could be seen from research methods. The methods currently used are mainly experiment, psychological measurement and assessment, and theoretical reasoning. There is a rapid increase of research by using scales. Wechsler Intelligence Scale for Children, Binet Scale and Baley Scale have been revised andstandardized. Chinese researchers have also developed several good scales of their own, some of which are valuable and need to be standardized. In the papers investigated, the amount of citation is significantly lower than the world average level as well as the average citation number of whole China's scientific literature. Among the papers cited, most are of Chinese and English languages, and only a small rate were published in resently five years. The renewal of literature cited seems to stay at a low level in the ten years. Tremendous work could be reflected by the number of subjects used the research work in those ten years: 362665. A lot of studies piled on the period of 4-16 year olds. Compared with the previous thirty years, the age range was much enlarged and there were quite a few studies about preschool, school and adolescent periods. The study of newborn of 0-3 has been a weak point so far and it is a field to which chinese developmental psychologists should pay more attention. The progress in using statistics is one of the most obvious part in the development in the research work of child developmental and educational psychology. The one tendency that should be awared and avoid is to put the cart before the horse: seeking for more sophisticated statistic method while neglecting the meanings of research problems. 3. Citation analysis was used in selecting scholars who had great influence in the field of child developmental and educational psychology. Among the often cited and famous scholars, 31 are Chinese researchers and 12 are Western psychologists. The authoritative journal for child developmental psychology and educational psychology is Acta Psychologica Sinica.
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In low-level vision, the representation of scene properties such as shape, albedo, etc., are very high dimensional as they have to describe complicated structures. The approach proposed here is to let the image itself bear as much of the representational burden as possible. In many situations, scene and image are closely related and it is possible to find a functional relationship between them. The scene information can be represented in reference to the image where the functional specifies how to translate the image into the associated scene. We illustrate the use of this representation for encoding shape information. We show how this representation has appealing properties such as locality and slow variation across space and scale. These properties provide a way of improving shape estimates coming from other sources of information like stereo.
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The goal of low-level vision is to estimate an underlying scene, given an observed image. Real-world scenes (e.g., albedos or shapes) can be very complex, conventionally requiring high dimensional representations which are hard to estimate and store. We propose a low-dimensional representation, called a scene recipe, that relies on the image itself to describe the complex scene configurations. Shape recipes are an example: these are the regression coefficients that predict the bandpassed shape from bandpassed image data. We describe the benefits of this representation, and show two uses illustrating their properties: (1) we improve stereo shape estimates by learning shape recipes at low resolution and applying them at full resolution; (2) Shape recipes implicitly contain information about lighting and materials and we use them for material segmentation.