Characterization of monocarboxylate transporters (MCTs) expression in soft tissue sarcomas: distinct prognostic impact of MCT1 sub-cellular localization

Background: Soft tissue sarcomas (STSs) are a group of neoplasms, which, despite current therapeutic advances, still confer a poor outcome to half of the patients. As other solid tumors, STSs exhibit high glucose consumption rates, associated with worse prognosis and therapeutic response. As highly glycolytic tumors, we hypothesized that sarcomas should present an increased expression of lactate transporters (MCTs).Methods: Immunohistochemical expression of MCT1, MCT2, MCT4 and CD147 was assessed in a series of 86 STSs and the expression profiles were associated with patients' clinical-pathological parameters.Results: MCT1, MCT4 and CD147 were mainly observed in the plasma membrane of cancer cells (around 60% for MCTs and 40% for CD147), while MCT2 was conspicuously found in the cytoplasm (94.2%). Importantly, we observed MCT1 nuclear expression (32.6%). MCT1 and MCT4, alone or co-expressed with CD147 in the plasma membrane, were associated with poor prognostic variables including high tumor grade, disease progression and shorter overall survival. Conversely, we found MCT1 nuclear expression to be associated with low grade tumors and longer overall survival.Conclusions: The present work represents the first report of MCTs characterization in STSs. We showed the original finding of MCT1 expression in the nucleus. Importantly, opposite biological roles should be behind the dual sub-cellular localization of MCT1, as plasma membrane expression of MCT1 is associated with worse patients' prognosis, while nuclear expression is associated with better prognosis.

Investigation of the effect of movement and irrigation systems on temperature in the conventional drilling of cortical bone

We have compared the results of the external irrigation technique with those of a double irrigation technique with continuous intermittent movement. Maximum thermal measurements were made in the cortical part of 10 samples of bovine ribs during osteotomy to simulate the preparation of a surgical bed for the installation of dental implants at a depth of 10 mm Twenty specimens were drilled for each group: external irrigation and continuous movement (control group 1, CG1); external irrigation and intermittent movement (control group 2, CG2); double irrigation and continuous movement (test group 1, TG1); and double irrigation and intermittent movement (test group 2, TG2). The double irrigation technique gave significantly better results regardless of the drilling movement used. Thermal increases between samples was 19.2% in group CG1, 10.4% in CG2, 5.4% in TG1, and 3.4% in TG2. The double irrigation technique produced a significantly smaller increase in temperature in the cortical bone during both types of drilling (p = 0.001), which illustrated its greater efficiency compared with that of the external irrigation technique. (C) 2013 The British Association of Oral and Maxillofacial Surgeons. Published by Elsevier Ltd. All rights reserved.

Physiological changes in eucalyptus hybrids under different irrigation regimes

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Double-lumen catheter in the right jugular vein induces two sub-endothelial abscesses in an unusual place, the transition between the superior vena cava and the right atrium: a case report

Endocarditis is a type of infection that is common in internal medicine wards and in haemodialysis clinics. The location that is most affected are the heart valves. Herein, we report a case of an uncommon abscess, a sub-endothelial abscess between the transition of the superior vena cava and the right atrium. There were several emboli to the lung and foot, and the agent was related to Staphylococcus aureus and a double-lumen catheter. Usually, this type of abscess is located in valves, either the tricuspid valve if related to catheters or injection drug use or the mitral valve if related to other causes. An exhaustive review was made, but we found no information about the location of this abscess and the rarity of the event motivating the report of infection.

Avaliação espaço-temporal da distribuição de fósforo na água e nos sedimentos de fundo das sub-bacias dos rios Cuiabá e São Lourenço e em algumas baias do Pantanal Matogrossense

Pós-graduação em Química - IQ

Avaliação da resposta imune sistêmica e compartimentalizada em pacientes portadoras de cãncer de ovário: sub-titulo / (se houver)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

O caminho novo: o Vale Histórico da Serra da Bocaina - opulência e decadência da sub-região paraibana paulista (reintegração de um espaço geográfico 'deprimido')

A pesquisa resgata o trecho paulista do “Caminho Novo”, antiga estrada geral de São Paulo, ligação terrestre com o Rio de Janeiro, a partir do século XVIII. No chamado Vale Histórico da Serra da Bocaina, cujo relevo se apresenta muito movimentado, a cultura do café penetrou em território paulista. Esta sub-região do Vale do Paraíba tornou-se uma das mais prósperas do país. A fase posterior, de queda na produção cafeeira, transformou-a numa região “deprimida” e estagnada. Atualmente, existem esforços para, através dos diversos ramos do turismo, reativar e dinamizar esta região vale-paraibana paulista, de modo a reintegrá-la ao pujante sistema econômico de São Paulo.

Effect of Rotary Instrument Associated with Different Irrigation Techniques on Removing Calcium Hydroxide Dressing

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

SILICON IN ROW AND NITROGEN IN TOPDRESSING FERTILIZATION ON RICE UNDER DRY LAND AND SPRINKLER IRRIGATION CONDITIONS

The objective of this work was to evaluate the effects of silicon application adjusted with nitrogen fertilization via top-dressing on grain productivity, the silicon contents of the soil, in the plant tissue and nitrogen contents in dry and irrigated conditions. The experimental outlining was from designed blocks with subdivided parcels and four repetitions. The treatments consisted of culture system (dry and irrigated) and the under parcels by the combination of silicon (0 and 100 kg ha(-1)), in magnesium and calcium silicate form (with 23% of SiO2), and four doses of N (urea) via top-dressing (0, 30, 60 and 90 kg ha(-1)). Silicon application at sowing furrow was a viable technique because it provided significant increase in the content of this element in the root growth of rice. The application of silicon in the sowing furrow did not change the content of the element nor the nitrogen nutrition in rice plants. The nitrogen application reduced the silicon content and increased nitrogen nutrition in rice plants. Silicon application at sowing furrow provided no increase in rice grain yield. When there was no water limitation to nitrogen fertilization enhanced linearly on rice grain yield, whereas under water stress the effect of nitrogen fertilization was limited.

Caracterização hidroquímica dos sistemas aquíferos Serra Geral e Guarani da sub-bacia do Rio Pardo e municípios adjacentes, no sudoeste do estado de São Paulo

The main purpose of this work is to evaluate the chemical composition of the groundwaters from Guarani Aquifer System and Serra Geral Aquifer System in the Rio Pardo basin, located at the southwestern of São Paulo state. To the hydrochemical characterization, physicalchemical analyses from 32 sampled wells were used. These results were plotted in Piper diagrams allowing the classification of groundwater as calcium or calcium-magnesium bicarbonate type and sodium bicarbonate type for both aquifer systems. Distribution of hydrochemical facies over the study area was obtained using Stiff diagrams. The groundwater can be classified, representing in this sequence its hydrochemical evolution. The recognized mechanisms responsible for groundwater evolution are dissolution of minerals magnesium, such as olivine, present in the basalt, dissolution of feldspars and removal of the carbonate cement of the sandstones mineral framework. Ionic exchange may represent an important processes in the groundwater evolution, responsible for the increase in the sodium concentration and decrease of calcium

Efeito da prática do futebol no desempenho em agilidade, velocidade e coordenação de meninos na categoria dentinho ou sub 11 (10 e 11 anos de idade)

In Brazil, the practice of Soccer is encouraged very early. It is common remark that parents enroll their sons into soccer schools, promoting the involvement of these children in formal practice. However, there are not many studies that assess the effects of this practice in the physical capacities of children. Thus, the question that guides this study is: children who practice soccer systematically present better development of physical, especially agility, coordination and speed? The objective of this study was to compare the performance in velocity tests, agility and coordination of boys of 10 and 11 years, category dentinho or Sub-11, practitioners of soccer in schools and non practicing. The study included male children, born in 1997 and 1998, formed two groups: group of practitioners of soccer and group of non practicing. To belong to the group of practitioners, the child was regularly engaged for at least 1 year, with 1 hour daily and 2 to 3 times per week. The participants of the group of non practicing were not involved in regular practice of soccer and were paired in weight and stature with participants of the group of practitioners of soccer. Each participant was individually assessed in neutral environment and without foreign interference. For the assessment of the agility, was utilized the (shuttle run) test. The race test 30 meters stopped was employed for the assessment of the speed. The test of fiddling between the cones was employed to measure the coordination. The time spent to perform 1 attempt to each test was used for the comparison between groups. These data were treated, initially, by descriptive statistics (mean and standard deviation) and, subsequently, were compared by means of Anova, for independent samples. The significance level was maintained at 5%. Through analysis of the results the conclusion is that the regular practice ...(Complete abstract click electronic access below)

Carta geotécnica das sub-bacias dos córregos do sossego e do barracão nos municípios de Americana e Santa Bárbara D'Oeste na escala 1:20.000

The Geotechnical Mapping is an extreme important tool for the urban planning, so the public authorities can establish guidelines for medium and long term for a better life conditions for the population. The division region between the cities of Americana and Santa Bárbara D’Oeste has an intense conurbation problem, which is causing problems of occupation in risks areas on environmental terms. In this context this project has as an objective the elaboration of a Geotechnical Map from the sub- basins of Sossego and Barracão rivers, whicht are located in between the area of the two cities, and presents some of the process causes of the conurbation problem. For this study, the following steps were proposed: bibliographic and topographic maps research for better knowledge from the theme and the area, and also preparing cartographic basis; making of preliminary geotechnical area map with a 1:10.000 scale, gather all the information acquired at the previous step and compartmentation of the area; field step to collect more information and soil for the laboratory analysis, objecting the characterization of the geotechnical unities; lab analysis; final geotechnical map preparation; and final report elaboration. The final product of this study is the Final Geotechnical Map, with a 1:10.000 scale, and the principal objective is to help the urban planning

A cunha do ciclo de fácies do Grupo Guatá (Eopermiano) na margem sub-aflorante do norte paranaense

A wedge facies cycle it‟s a body of sedimentary rock, bounded above and below by unconformity and which represents a transgressive-regressive cycle of deposition. This cycle is formed by the following succession of facies: non-marine (N), transitional-coarse texture, and marine-fine texture; The objective of this study have been members of the sandstones and Triumph Siderópolis, seconded by rare calcareous sandstones and very thin limb Paraguaçu. Through analysis of facies in wells testified by CPRM, it was possible to group on facies successions and set the standard stacking. The Triunfo Member consists of three cycles of facies, lithology whose standard indicates a vertical arrangement ''type'' tuning up, other words, a component sandy bottom in the form of '' box'', followed by components sandy-clayey and clayey-carbonaceous (1st and 2nd cycle) and / or shale and limestone (3rd cycle), represented by the base member Paraguaçu. The first cycle only occurs on NF-5 wells and NF-6 in the form of Onlap channel dug in post-Itararé group. The cycle II also reaches wells NF-4 and NF-3 to the north, while the cycle III reaches the southern area, well NF-9. The member Paraguaçu, well PP-25 is predominantly clay-síltico, also stand out limestones and sandstones. The limestones are not coastal marine organisms probably connected with the algae and cyanobacteria, the sandstones are fine-textured, very thin and coastal deposition environment. The member Siderópolis, well NF-2 consists of a sandstone fluvial-estuarine deployed in the valley section, and succeeded by sandstones and siltstones bioturbed, they have linked the transgression of the Fm. Palermo

Geração e análise da imunogenicidade de plasmídeos contendo epítopos CD8 sub-dominantes expressos em Trypanosoma cruzi

Durante a infecção experimental por Trypanosoma cruzi, a resposta imune adaptativa mediada por células CD8+ é direcionada a poucos epítopos imunodominantes e subdominantes. Em estudos anteriores, foi demonstrado que, em diferentes linhagens de camundongos, a imunização com plasmídeos ou proteínas recombinantes, que expressam epítopos CD8 imunodominantes, antecipa a resposta imune após o desafio e proporciona resposta imune protetora contra infecções letais com T.cruzi. Em nosso estudo atual, desenvolvemos a hipótese que também é possível gerar resposta imune protetora a partir de imunizações com plasmídeos que expressam epítopos subdominantes. Neste caso, esta resposta poderia se somar a resposta imune dominante ampliando o espectro da resposta imuno-protetora. Para testar nossa hipótese, nós tivemos que construir diferentes plasmídeos contendo a ORF do gene da proteína 2 de superfície de amastigota (asp-2) de T.cruzi contendo mutações que: i) retiraram os epítopos imunodominantes CD8 (VNHRFTLV para H-2Kb ou TEWETGQI para H-2Kk) ;ii) trocaram o epítopo imunodominante por epítopos sub-dominantes (TsKb-18 ANYDFTLV ou TsKb-20 ANYKFTLV). Além disso, também foi construído um plasmídeo contendo somente a porção P4-P7 (261° a 500° aminoácidos) da asp-2. Após a construção destes plasmídeos, testamos a imunogencidade de dois deles, denominados pRE-P4P7 e o pIgSP-VNHRATLA, foram testados individualmente na vacinação de camundongos F1(C57BL/6 x B10.A) (H-2Kb e H- 2Kk) ou de CB10 (H-2Kb), respectivamente, e desafiados com tripomastigotas da cepa Y de T.cruzi. A vacinação de camundongos F1 (C57BL/6 x B10.A) com o plasmídeo pré- P4-P7 aumentou significativamente a resposta imune de células CD8+ específicas ao epítopo sub-dominante TEWETGQI, restrito ao MHC H-2kk, porém não diminuiu a resposta ...(Resumo completo, clicar acesso eletrônico abaixo)