Platelet-rich plasma (PRP): methodological aspects and clinical applications

The clinical use of platelet-rich plasma (PRP) is based on the increase in the concentration of growth factors and in the secretion of proteins which are able to maximize the healing process at the cellular level. Since PRP is an autologous biologic material, it involves a minimum risk of immune reactions and transmission of infectious and contagious diseases, and it has been widely used for the recovery of musculoskeletal lesions. Despite the great potential for applicability, the implementation of the therapeutic employment of PRP as a clinical alternative has become difficult, due to the lack of studies related to the standardization of the techniques and/or insufficient description of the adopted procedures. Therefore, it is required establish standard criteria to be followed for obtaining a PRP of high quality, as well as a larger number of studies which should establish the proper concentration of platelets for the different clinical conditions. In this context, the purpose of this review is to discuss some methodological aspects used for achieving the PRP, as well as to discuss the bioactive properties of PRP, and to point out its therapeutic use in different fields of regenerative medicine.

Dissidências existenciais de gênero: resistências e enfrentamentos ao biopoder

This article approaches the questions about the sexes, genders, desires and sexual standardization practices, starting from the appearance of new sexual identities that can be classified as queer – travesty, transsexual and transgender people. Still, we problematize how the manners of established forms of subjectivity and the hegemonic institutions marginalize such forms of existences, because of the immense difficulty in the encounter with the stranger, the different and the unusual. With those analyses, we are trying to provide the creation of devices to reduce the intensities of the discrimination, of the stigmatization, of the violence, so frequently lived by the people dissident from the heterosexual gender normative, what was reported about their own lived experiences.

Estudo normativo do R-2: teste não verbal de inteligência para crianças

This study presents the standardization of the R-2 Non Verbal Intelligence Test for Children conducted at the city of Assis – SP, Brazil, and compares it with the São Paulo city standardization. The sample was composed by 559 children, between 5 and 11 years old, half of each sex, students from Assis city, randomly selected according to their proportion in private and public schools. Results indicate differences between ages and school types, but not between sexes. Percentile norms were established for the total sample at each age. The comparison of Assis and São Paulo city children reveals significant differences and Assis' results slightly higher. The conclusion is that R-2 Test is appropriate to cognitive assessment of Assis children, suggesting the use of new norms for this region.

Neovascularization after ischemic injury: evaluation with 99mTc-HYNIC-RGD

Purpose: Angiogenesis involves many mediators including integrins, and the tripeptide RGD is a target amino acid recognition sequence for many of them. Hindlimb ischemia is a simple and convenient animal model however standardization of the injection procedures in the devascularized and control limb is lacking, thus rendering difficult the interpretation of results. The aim of this investigations was to evaluate neovascularization in a hindlimb murine model by means of 99mTc-HYNIC-ß-Ala-RGD. Methods: 99mTc-HYNIC-RGD analog was prepared using coligands. Ischemia was induced in Wistar rats by double- ligation of the common femoral artery. Radiolabeled RGD was injected after 2h, as well as 1, 3, 5, 7, 10 and 14 days. Uptake was evaluated by planar imaging and biodistribution studies. Results: The highest ratio between ischemia and control was achieved at the 7th day (2.62 ± 0.95), with substantial decrease by the 14th day. For pertechnetate the 7th day ratio was 0.87 ± 0.23. Scintigraphic image confirmed different uptakes. Conclusion: 99mTc-HYNIC-RGD analog concentrated in ischemic tissue by the time of widespread angiogenesis and pertechnetate confirmed reduction in blood flow. In this sense, the protocol can be recommended for ischemic models.

Empacotamento de experimentos controlados em engenharia de software: uma abordagem baseada em ontologia

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Citocompatibilidade, propriedades antibiofilme e físicas e cimentos endodônticos associados à amoxilina

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Influência de tratamentos de superfície e agentes cimentantes na sobrevivência de coroas de Y-TZP recobertas por porcelana

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Colostro e leite de éguas: análise mirobiológica e contagem de células somáticas

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Renovando o mundo: cura através da união na diversidade : um plano de comunicação para o pacto mundial consciente

The perception of modern society regarding the nature and indigenous peoples came to be shaped in the sixteenth and seventeenth centuries, with two major events, the invasion of Europeans in America and the scientific revolution. These events symbolize the cultural overlap and European thinking about the rest of the world. The current scenario is industrialism, consumism and cultural standardization driven by Cartesian thought. This work demonstrates, through literature research and data analysis, that this dominant model has caused the crisis of modernity, focusing especially on the environmental crisis that threatens human life. The solutions are present in the modern society the knowledge of indigenous peoples that is inherently ecological and sustainable by tradition. From the union of diverse cultures and knowledge dialog you can find solutions for today's problems and provide a renewal of relationships between modern society, indigenous peoples and nature. The World Conscious Pact presents this proposal which can be increased through public relations strategies in order to reconnect the man-nature bond, generating critical behavior with respect to the current dominant system and motivate society to adopt truly ecological postures. For this it is necessary to rescue the Earth image as a mother

Fluorescence Intensity of Composite Layering Combined with Surface Sealant Submitted to Staining Solutions

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Análise acústica em brinquedos ruidosos

PURPOSE: to analyze the levels of noise emitted by nationally-sold toys for use by children from 1 to 5 year old; to compare the values among the toys with and without the seal of Inmetro (National Institute of Metrology, Standardization and Industrial Quality) and to analyze the sound spectrum of toys, in order to identify the area of the cochlea that may be more affected by these noises. METHOD: measurements were performed on 20 sound toys (10 with the seal of the Inmetro and 10 without the seal) with the use of digital sound level meter in an acoustically treated room, and the sound analysis was performed using the Praat program. RESULTS: toys placed at 2.5 cm from the equipment with the seal of the Inmetro had an intensity ranging from 61.50 to 91.55 dB (A) and from 69.75 to 95.05 dB (C), positioned at 25 cm ranged from 58.3 to 79.85 dB (A) and from 62.50 to 83.65 dB (C). The results of the toys without warranty stamps placed at 2.5 cm ranged from 67.45 to 94.30 dB (A) and 65.4 to 99.50 dB (C) and the distance of 25 cm recorded from 61. 30 to 87.45 dB (A) and 63.75 to 97.60 dB (C), so that the findings demonstrated that there are noisy toys that go beyond the values recommended by the current legislation in both groups, with and without warranty stamps . CONCLUSION: the toys without the seal of Inmetro showed intensities values significantly higher than the other group, offering more risk to the children s hearing health.

Taenia saginata: polymerase chain reaction for taeniasis diagnosis in human fecal samples

The taeniasis-cysticercosis complex is a zoonosis of great medical and economic importance where humans play an important role as the carrier of adult stage of Taenia solium and Taenia saginata. This paper describes PCR standardization that can be applied in human fecal samples for taeniasis diagnosis. DNA extraction was achieved with DNAzol reagent, after egg disruption with glass beads. DNA prepared from fecal specimens was first purified and PCR amplified generating fragments of 170 and 600 bp. The assay described herein provides an important tool for T. saginata identification in human fecal samples.

Areas hidrologicamente homogêneas como subsídio ao manejo de bacias hidrográficas

Pós-graduação em Agronomia (Irrigação e Drenagem) - FCA

Articulações territoriais: uma cartografia da atenção à população em situação de rua

Pós-graduação em Psicologia - FCLAS