A essência do Arbutus unedo: caraterização morfológica e genética do medronheiro de Castelo de Paiva

O medronheiro é um arbusto da região mediterrânica que pode ser encontrada por todo o país. Ao contrário do que verifica na região sul do país, no concelho de Castelo de Paiva é atribuída uma reduzida importância económica a esta espécie. Com o intuito de preservar e potenciar a produção desta espécie e contribuir para a dinamização da economia do concelho, procedeu-se à caracterização morfológica e genética de uma amostra da população de medronheiros de Castelo de Paiva. A caracterização morfológica e genética foi realizada para um total de 10 genótipos. Para tal recolheram-se 70 folhas aleatoriamente em cada árvore. Em 40 folhas mediu-se o comprimento, largura, comprimento do pedúnculo, peso fresco, peso seco e determinou-se a área foliar. Dos caracteres morfológicos analisados, aqueles que se revelaram mais úteis na distinção dos vários genótipos foram: comprimento do pedúnculo, peso fresco e peso seco. As restantes 30 folhas foram utilizadas para a caracterização genética. Esta caracterização foi realizada recorrendo a um marcador de DNA, ISSR. Os 5 primeiros exemplaresutilizados na técnica de ISSR demonstraram-se polimórficos. Os resultados da caracterização genética sugerem que a variabilidade genética na população é média a alta.

Amaurose congénita de Leber : apresentação clínica, genética e novas estratégias terapêuticas

Trabalho Final do Curso de Mestrado Integrado em Medicina, Faculdade de Medicina, Universidade de Lisboa, 2014

Surdez genética na idade pediátrica : O papel da genética na abordagem à doença

Trabalho Final do Curso de Mestrado Integrado em Medicina, Faculdade de Medicina, Universidade de Lisboa, 2014

Análise genética com Y-SNPs de subgrupos populacionais residentes no Sul de Portugal

Tese de mestrado, Medicina Legal e Ciências Forenses, Universidade de Lisboa, Faculdade de Medicina, 2016

Salir de casa para volver a casa : lectura de la genética autoficcional de Alejandro Zambra (2006-2011)

Ce travail est une analyse exhaustive des romans publiés par l’écrivain chilien Alejandro Zambra entre 2006 et 2011. Compte tenu de son appartenance à la génération postdictatoriale, on va se concentrer sur la reconstruction autofictionnelle de l’enfance, et sur la façon dont elle reproduit, questionne et renverse le récit dictatorial dominant qui relègue les enfants sur le plan des personnages secondaires. Pour identifier le développement du discours, on va s’appuyer sur des outils critiques de la génétique littéraire dont le concept central « d’avant-texte » nous permet de faire une lecture rétrospective de Bonsái et de La vida privada de los árboles comme des précurseurs idéologiques de Formas de volver a casa. En raison de la centralité jouée par l’espace dans ces romans, on va consacrer une grande partie du travail à la représentation spatiale et la manière dont l’espace configure la position des personnages à l’intérieur du schème narratif. C’est dans cette relation que l’on identifie la dichotomie spatiale élaborée dans les œuvres, car l’espace et la vie privée sont reliés à l’inertie et la résignation dans l’univers textuel, tandis que l’action et le questionnement appartiennent au domaine de l’espace et la vie publics. Lus l’un après l’autre, les romans témoignent du parcours suivi de l’écrivain, qui assimile le récit dictatorial en évitant la confrontation narrative au début, pour, par la suite, approcher le passé, l’histoire familiale et la dictature, dans un processus autofictionnel qui projette les différentes voies du retour chez soi.

Salir de casa para volver a casa : lectura de la genética autoficcional de Alejandro Zambra (2006-2011)

Ce travail est une analyse exhaustive des romans publiés par l’écrivain chilien Alejandro Zambra entre 2006 et 2011. Compte tenu de son appartenance à la génération postdictatoriale, on va se concentrer sur la reconstruction autofictionnelle de l’enfance, et sur la façon dont elle reproduit, questionne et renverse le récit dictatorial dominant qui relègue les enfants sur le plan des personnages secondaires. Pour identifier le développement du discours, on va s’appuyer sur des outils critiques de la génétique littéraire dont le concept central « d’avant-texte » nous permet de faire une lecture rétrospective de Bonsái et de La vida privada de los árboles comme des précurseurs idéologiques de Formas de volver a casa. En raison de la centralité jouée par l’espace dans ces romans, on va consacrer une grande partie du travail à la représentation spatiale et la manière dont l’espace configure la position des personnages à l’intérieur du schème narratif. C’est dans cette relation que l’on identifie la dichotomie spatiale élaborée dans les œuvres, car l’espace et la vie privée sont reliés à l’inertie et la résignation dans l’univers textuel, tandis que l’action et le questionnement appartiennent au domaine de l’espace et la vie publics. Lus l’un après l’autre, les romans témoignent du parcours suivi de l’écrivain, qui assimile le récit dictatorial en évitant la confrontation narrative au début, pour, par la suite, approcher le passé, l’histoire familiale et la dictature, dans un processus autofictionnel qui projette les différentes voies du retour chez soi.

Un dios que se transforma en héroe : Una aproximación genética a los textos de Peter Pan

Desde la perspectiva de la genética textual, en este trabajo observamos las representacionesdel personaje de Peter Pan en los textos de J. M. Barrie dedicados a este con respecto a sussemejanzas con el dios griego Pan, para ver de qué manera dialogan con los diferentes sentidosque el dios fue connotando a través de siglos. Analizamos la fotonovela The Boys Castawaysof Black Lake Island (1901); The Little White Bird (1902), novela en la que aparece en seiscapítulos; las "Fairy Notes" (1903) en borrador y el libreto inédito llamado Anon: a play de la obra teatral Peter Pan or The Boy Who Would Not Grow Up (estrenada en 1904); la novela Peter Pan in Kensington Gardens (1906), que consiste en esos seis capítulos de The LittleWhite Bird; la novela Peter and Wendy (1911), adaptación de la obra de teatro; un guion cinematográfico,Scenario for a Proposed Film of Peter Pan (c. 1918), que no se filmó; y ellibreto de la obra, finalmente publicado, con bastantes cambios, en 1928

Un dios que se transforma en héroe : Una aproximación genética a los textos de Peter Pan

Desde la perspectiva de la genética textual, en este trabajo observamos las representacionesdel personaje de Peter Pan en los textos de J. M. Barrie dedicados a este con respecto a sussemejanzas con el dios griego Pan, para ver de qué manera dialogan con los diferentes sentidosque el dios fue connotando a través de siglos. Analizamos la fotonovela The Boys Castawaysof Black Lake Island (1901); The Little White Bird (1902), novela en la que aparece en seiscapítulos; las "Fairy Notes" (1903) en borrador y el libreto inédito llamado Anon: a play de la obra teatral Peter Pan or The Boy Who Would Not Grow Up (estrenada en 1904); la novela Peter Pan in Kensington Gardens (1906), que consiste en esos seis capítulos de The LittleWhite Bird; la novela Peter and Wendy (1911), adaptación de la obra de teatro; un guion cinematográfico,Scenario for a Proposed Film of Peter Pan (c. 1918), que no se filmó; y ellibreto de la obra, finalmente publicado, con bastantes cambios, en 1928

Un dios que se transforma en héroe : Una aproximación genética a los textos de Peter Pan

Desde la perspectiva de la genética textual, en este trabajo observamos las representacionesdel personaje de Peter Pan en los textos de J. M. Barrie dedicados a este con respecto a sussemejanzas con el dios griego Pan, para ver de qué manera dialogan con los diferentes sentidosque el dios fue connotando a través de siglos. Analizamos la fotonovela The Boys Castawaysof Black Lake Island (1901); The Little White Bird (1902), novela en la que aparece en seiscapítulos; las "Fairy Notes" (1903) en borrador y el libreto inédito llamado Anon: a play de la obra teatral Peter Pan or The Boy Who Would Not Grow Up (estrenada en 1904); la novela Peter Pan in Kensington Gardens (1906), que consiste en esos seis capítulos de The LittleWhite Bird; la novela Peter and Wendy (1911), adaptación de la obra de teatro; un guion cinematográfico,Scenario for a Proposed Film of Peter Pan (c. 1918), que no se filmó; y ellibreto de la obra, finalmente publicado, con bastantes cambios, en 1928

A Engenharia Genética como Proposta de Co-Criação vista a Partir do Pensamento do Teólogo Ronald Cole-Turner

Esta dissertação de mestrado busca pesquisar a relação estabelecida pelo teólogo Ronald Cole-Turner, entre a engenharia genética e o conceito de co-criação , desenvolvido como uma alternativa para que se pense o papel do ser humano frente aos desenvolvimentos tecnológicos atuais. Tendo em vista o conceito da evolução, da criação contínua e dos potenciais gerados pela engenharia genética, Cole-Turner defende que o ser humano pode participar das atividades criativas de Deus por meio desta tecnologia, desde que priorize os aspectos redentivos que podem ser extraídos de seu uso. Faz isto em demonstrar, por meio do relato Jawista da criação, como o agir criativo de Deus está relacionado ao agir humano, através de suas tecnologias, e em relacionar os milagres de cura operados por Jesus nos evangelhos, como explicitação epistêmica da vontade de Deus, que pode ser participada pelos seres humanos. O objetivo básico desta pesquisa será o de relatar, no primeiro capítulo, o aspecto teórico da engenharia genética, nas suas possibilidades positivas e negativas. Em seguida, no capítulo segundo, se fará uma exposição do pensamento de Cole- Turner, em diálogo com outros interlocutores do tema. Por fim, no último capítulo, se verificará as respostas oferecidas às várias observações críticas que seu pensamento recebe. A hipótese é a de que, se a criação é um projeto contínuo e inacabado, e o destino dos seres humanos se encontra inserido dentro desta continuidade, seria coerente afirmar que estes possam participar das atividades de Deus em tais projetos. Como a evolução biológica é uma dimensão da criação, que ocorre por meio de transformações no capital genético dos organismos vivos, os seres humanos, tendo conhecimento e habilidade para repetir os processos verificados nestas modificações, poderão empreender seus esforços, tanto na área da técnica, como na da ética, para participarem da consecução dos propósitos de Deus.(AU)

Conservação genética da orquídea Cattleya granulosa Lindley

Cattleya granulosa Lind is a large and endemic orchid in Atlantic Forest fragments in Northeast Brazil. The facility of collecting, uniqueness of their flowers, which have varying colors between green and reddish brown, and distribution in coastal areas of economic interest make their populations a constant target of predation, which also suffer from environmental degradation. Due to the impact on their populations, the species is threatened. In this study, we evaluate the levels of spatial aggregation in a preserved population, analyze the phylogenetic relationships of C. granulosa Lindl. with four other Laeliinae species (Brassavola tuberculata, C. bicolor, C. labiata and C. schofieldiana) and also to evaluate the genetic diversity of 12 remaining populations of C. granulosa Lindl. through ISSR. There was specificity of epiphytic C. granula Lindl. with a single host tree, species of Eugenia sp. C. granulosa Lindl. own spatial pattern, with the highest density of neighbors within up to 5 m. Regarding the phylogenetic relationships and genetic patterns with other species of the genus, C. bicolor exhibited the greatest genetic diversity (HE = 0.219), while C. labiata exhibited the lowest level (HE = 0.132). The percentage of genetic variation among species (AMOVA) was 23.26%. The principal component analysis (PCA) of ISSR data showed that unifoliate and bifoliolate species are genetically divergent. PCA indicated a close relationship between C. granulosa Lindl. and C. schofieldiana, a species considered to be a variety of C. granulosa Lindl. by many researchers. Population genetic analysis using ISSR showed all polymorphic loci. The high genetic differentiation between populations (ФST = 0.391, P < 0.0001) determined the structure into nine groups according to log-likelihood of Bayesian analysis, with a similar pattern in the dendrogram (UPGMA) and PCA. A positive and significant correlation between geographic and genetic distances between populations was identified (r = 0.794, P = 0.017), indicating isolation by distance. Patterns of allelic diversity suggest the occurrence of population bottlenecks in most populations of C. granulosa Lindl. (n = 8). Genetic data indicate that enable the maintenance of genetic diversity of the species is complex and is directly related to the conservation of different units or groups that are spatially distant.

Açude do cais: uma proposta de aplicação de uma sequência de atividades didáticas em um contexto real

Currently it is expected that science education will enable a more comprehensive conception of the world and of the relationship between scientific knowledge, technology and society. Thus, we seek the science teaching attend around contexts related to science, technology and society (STS). According CTS approaches, the science education should promote learning in the scientific, technological and social fields, from experienced real contexts, generating motivation to students, offering them tools to work as scientifically literate citizens. For this, it becomes crucial to resort to innovative activities and the various methodologies and appropriate teaching materials, and there is a lack of this perspective in general basic education, which drives the practice of an STS approach. Therefore, the aim of this research is the production of an instructional sequence for the exploration of the theme: "The quality of the water in the municipality of Cuité-PB," according to focus CTS in the 2nd year of high school. The choice of this topic is due to the problem caused by the quality of water in this municipality, with the concern to address an issue that came to work the critical / reflective understanding of a real context, in order to sensitize students to the importance of scientific / technological knowledge. The selection criteria of STS subjects were also observed. In this research the materials were produced and applied by the teacher / researcher. For data collection we used the observation and analysis of student records. The results were encouraging in the sense of participation, motivation and skill acquisition. This research contributes to the teaching of science with a focus STS, as well as encourage the use of perspective in new contexts with real questioning

Inércia e Natureza da Ciência no Ensino de Física: uma sequência didática centrada no desenvolvimento histórico do conceito de inércia

During the last decades the area of science education has discussed issues related to the inclusion of the History and Philosophy of Science (HFS) in the practice of science teaching. Among the arguments put forward in favour of this approach, it is pointed out the possible enhancement of scientific content learning and the understanding of the nature of Science (NoS). In spite of such considerations, we still have a very small number of research papers reporting results of practical interventions that utilize the historical approach, moreover, there is a lack of teaching materials in this perspective. Our work has sought to contribute to this area with regard to two aspects: on the one hand, with the production of didactic material, by drawing up texts on the history of inertia for graduate students. On the other hand, we investigate whether the arguments mentioned above in relation to the didactic use of HFS sustain themselves, in a particular context. We developed and applied a didactic sequence, using the texts that we built, to teach the concept of inertia and discuss selected contents of NoS. The didactic sequence was applied in two graduate classes, one from a course of Geophysics (BSc.) and another from the Physics (teaching formation), both from the Federal University of Rio Grande do Norte (UFRN). An initial survey exposed that students, even having approached the concept of inertia in basic education, presented conceptions of common sense regarding the relationship between force and motion. The questionnaire also allowed us to identify the existence of elements of concepts considered inadequate as regards to NoS. At the end of our research, our data indicated a greater number of positive hits on the issues concerning the concept of inertia. Regarding the aspects of NoS, we were able to identify, in a few cases, a move towards a more appropriate understanding, however, certain distortions persisted, highlighting the limitations of the approach used

Expressão heteróloga de biossurfactantes identificados em bibliotecas metagenômicas

The microorganisms have a vast genetic diversity and they are present throughout the biosphere, however, only about 1% of the species can be cultivated by traditional cultivation techniques. Within this diversity there is a huge pool genetic and biological being explored. The metagenomics has enabled direct access to microbial genome derived from environmental samples using independent methods of cultivation. The methodology enables to obtain functional information about the proteins, as well as identify potential products with biotechnological interest and new industrially exploitable biological resources, such as new solutions to environmental impacts. Oil-contaminated areas are characterized by a large accumulation of hydrocarbons and surfactants may be used for bioremediation. Thus, the metagenomic approach was used in this study in order to select genes involved in the degradation and hydrocarbon emulsification. In a previous work, the environmental DNA (eDNA) was extracted from soil samples collected from two different areas (Caatinga and Saline River) of Rio Grande do Norte (Brazil), the metagenomic libraries were constructed and functionally analyzed. The clone able to degrade the oil was evaluated for the ability to synthesize biosurfactants. The sequence analysis revealed an ORF with 897 bp, 298 amino acids and a protein with around 34 kDa. The search for homology in GenBank revealed sequence similarity with a hypothetical protein of representatives Halobacteriaceae family, who were recently shown as strains producing biosurfactants. The presence of the inserted coding sequence and the acquired phenotype was confirmed. Primers were designed and the ORF amplified by PCR. The ORF was subcloned into pETDuet-1 expression vector for subsequent purification of the protein of interest containing a histidine tail. The tests performed to confirm the biosurfactant activity and the ability of hydrocarbon degradation showed positive results. The immunodetection test (western blot) using the monoclonal AntiHis® confirmed the presence of the environmental protein. This study was the first to report a possible protein with biosurfactant activity obtained from a metagenomic approach