Influência da luz LED na cultura celular de Mieloma múltiplo murino–NS1 in vitro

A monoclonal antibody (mAb) is an important tool in medical biotechnology and the production of biopharmaceuticals, especially for disease diagnosis and treatment of infections, because the antibodies have a significant advantage over chemical agents used in conventional therapies . The last thirty years the technology of production of monoclonal antibodies developed mainly the technique of obtaining in vitro, but also of their production is laborious, the cost is high. A major element of the high cost of production is the fact that the long-term culture consumes a large amount of imported inputs with high added value. A major contribution of this work is to promote cell growth more quickly and efficiently. Currently, a great race to discover new technologies and techniques to synthesize new antibodies and significantly increase the production of murine mAbs. New technologies such as laser and LED are innovations and widespread in modern life, so much so that its use has proliferated worldwide, primarily in the medical field. Recent studies show a series of results from the influence of the LED light in biological tissues such as: increasing the rate of cell proliferation, increased production rate of fibroblasts, increasing the rate of synthesis of RNA and DNA synthesis of ATP, etc. To assess the contribution of the LED in the culture of Myeloma NS1murino compared to the standard procedure. - NS1 cells were provided and followed the criteria of culture medium of the Laboratory of Cellular Engineering Center of Botucatu (POPs). The same amount of cells was grown in bottles of 25 cm2 polystyrene Tissue Culture Treated, specifically marked and kept in special medium RPMI 1640 Gibco BRL  supplemented with fetal bovine serum 10%, essential amino acids and non-essential, glucose, insulin and antibiotics. It was used in LEDs Cromatek wavelength of 630nm, 475nm and 530nm. The groups were... (Complete abstract click electronic access below)

Aplicação do método desirability para otimização de processo com múltiplas respostas

Currently, the competition between organizations in the pursuit of consumer preference has become increasingly fierce. In addition, consumers have become increasingly demanding due to high speed with which innovations occur, leaving the companies meet and sometimes surpass those expectations In this context, there is the necessity to use methods as mathematical models capable of dealing with the optimization of multiple responses simultaneously. In this context, this study presents an application of techniques of Design of Experiment in a machining process of a NIMONIC 80 alloy, a “superalloy” that has thermal and mechanical properties that make its machining difficult and in order to do this, the Desirability Function was used. As they are determining conditions in the machining capability of the alloy, the roughness and the cutting length were considered as variable settings, and the factors that can influence them are cutting speed, feed rate, cutting depth, inserts type and lubrication. The analysis of the result pointed out how was the influence of all factors on each response and also showed the efficiency and reliability of the method

Dinâmica e estratégia tecnológicas na indústria de telequipamentos: uma abordagem a partir da economia evolucionária

As tecnologias de informação e comunicação (TICs), em especial, o setor de telefonia passou e, ainda, passa por mudanças importantes: o aumento do número de características incorporadas aos aparelhos celulares. Isto moldou o ambiente competitivo de alta concentração, através de aquisições das empresas (e ativos tecnológicas) e da emergência de “novos” competidores. Buscou-se analisar a trajetória recente da indústria de telequipamentos, no que diz respeito às estratégias das empresas. Bem como identificar tendências tecnológicas e o desenvolvimento de novas tecnologias

Desenvolvimento de software livre/código aberto no Brasil

O presente trabalho propõe-se a discutir os determinantes do processo de desenvolvimento de software livre, seus efeitos na indústria de software e as ações do governo em relação a estas iniciativas Impulsionados, principalmente, pelos impactos dos avanços nas tecnologias de informação e comunicação e por inovações de processos relacionados a estes progressos, projetos de desenvolvimento de Software Livre/Código Aberto vêm crescendo em ritmo cada vez mais acelerado e tornando-se alvo de estratégias de empresas e políticas públicas. A partir desta constatação, o trabalho procura relacionar o arcabouço teórico que visa explicar estas iniciativas com dados da indústria brasileira de software e verificar como este modelo de produção pode modificar e agregar às suas feições. Foi realizado também um balanço das intervenções do governo brasileiro relacionadas a software livre/código aberto

O uso do youtube como ferramenta no ensino da química: análise de vídeo

Contemporary society is going through a cultural revolution evidenced by the area of information technology, and this has led to a transformation in social relations and in the classroom. trying to adjust to these changes and also to curricular high school, the teacher can use as teaching materials, some visual aids: the videos. for this he can use the youtube site to select this educational material. however, given the vast amount of videos available on the site requires a careful selection of them. this work of completion then you want to make a qualitative analysis about the videos available free on the youtube site

Otimização da extração alcalina com peróxido de hidrogênio em seqüência de branqueamento com aplicação de baixo fator kappa

The new market, focused on sustainability and other environmental concerns, refers to innovations that seek alternative forms of production. In pulp and paper bleaching alternative reagents are studied, for example, hydrogen peroxide, in partial substitution of chlorine dioxide in order to reduce the formation of organochlorines. In this context, this study examined the burden of hydrogen peroxide (H2O2) on alkaline extraction stage (stage Ep) required for the bleaching of pulp with eucalyptus kraft pulp, pre-oxygen delignified to obtain equivalent brightness at 90 ± 0.5% ISO, as well as its effect on quality of pulp produced. The pulp was bleached by the sequence D(Ep)DP, with the application of factor kappa of 0.14 and varying the concentration of hydrogen peroxide in Ep stage three, five, seven and nine kilograms of reagent per ton of pulp absolutely drought. The final P stage was optimized with the use of six, nine and twelve pounds of hydrogen peroxide per ton of absolutely dry pulp to achieve the required brightness. The quality of the pulp produced was analyzed based on the kappa number, the brightness and the viscosity. The methods were performed according to standards set by the standard TAPPI (Technical Association of the Pulp and Paper Industry). The best result was obtained using the following D0Ep(7)D1P(6), which showed a viscosity of 19.9 cP, 89.6% ISO brightness, consumption of 94.9 kg / t of reagents and reagent costs of US$ 28.15, because it showed better pulp quality for a lower cost compared to the others. It was found that the greater the amount of hydrogen peroxide in alkaline extraction, the lower the kappa number and increased the amount of residual hydrogen peroxide. The higher the charge of hydrogen peroxide in Ep stage, the lower the need for hydrogen peroxide in the final P stage, reducing the cost of bleaching

Proposta para a implantação de uma trilha interpretativa com bases na Pedagogia Waldorf na Floresta Estadual Edmundo Navarro de Andrade - Rio Claro, SP

At a time when people turn increasingly to technological innovations and virtual networks, a closer interaction with the environment can provide sensitizing experiences. One tool that facilitates this approach is the interpretative trail: its use in a Unit of Conservation (UC) allows more contact between the visitor and the nature elements. This research aimed to propose the establishment of an interpretative trail at the State Florest “Edmundo Navarro de Andrade” (FEENA) in Rio Claro (SP) with theoretical bases from Waldorf Pedagogy. The proposed trail is destined for students with 07-14 years-old, a period that the awareness is more accurate in a person, according to Waldorf tenets. On the path of the trail, we propose some educational activities at the points of interpretation: storytelling about environment; “photograph” the place with a drawing; create a song with sounds produced by the body and by nature elements; blindfold guide and a circle of people to share experiences. The activities aim to provide in the student a close and harmonious contact with oneself, with other people and with the natural world, and also serve as a support for programs of public use and environmental education at UC. The environmental awareness process in the context of FEENA - which has a significant landscape valuation - develops through the sensibilities of exterior and interior world. And also develops with the creation if images, thoughts, feelings and interdependencies relationships. Therefore, the ecologic education allows changes and formation of values and sensitive attitudes to students about the environment

Patentes patéticas

The objective of this project is to publish a book of scientific dissemination aimed at the subject of intellectual or industrial property, in particular patent, and its approval process. Based on a series of weekly blog posts published by its author, Patentes Patéticas use scientific, literary journalism and humour techniques to analyze a selection of U.S. patents that stand out for their questionable utility and criticize the number of patents granted as a metric of technological innovation in a country

Manejo pré-abate de bovinos

The pre-slaughter moment begins with the animal´s loading on the farm and ends up in its drain. It is a period in which the animal is under physical stress caused by hunger, thirst, fatigue, injuries, and extremes of temperature and psychological stress, due to restraint, handling, innovation and fear. Stress and inappropriate handling bring bad consequences for the final product´s quality, requiring better handling conditions, facilities and equipment. Examples of consequences of mishandling: meat with abnormal color, DFD meat (dark, firm and dry), reduced product´s shelf life, bruises and broken bones in carcasses. The operators should be trained and oriented on the basic principles of biology, behavior and management of cattle. At every step of the pre-slaughter moment, the animals should be handled calmly and quietly to avoid excitement and stress. Operators should avoid shouting, whistling or any noise since cattle have very sensitive ears and can become stressed. If the animals refuse to move, the cause of the problem should be determined

Comprimidos de HPMC contendo nimesulida: preparação, caracterização e estudo dos mecanismos que regem a liberação do fármaco

The pharmaceutical innovations, such as the use of polymers to control drug release, create possibilities for a better action of the drug in the body, which causes a a more effective therapeutic effect and a safer treatment for the patient. In this work, were prepared and characterized matrix tablets of hydroxypropylmethylcellulose (HPMC) containing nimesulide as model drug to evaluate the performance as a controlled release system. HPMC, a cellulose ester, is a hydrophilic polymer that undergoes swelling, i.e., absorbs water and forms a gel layer controlling drug release. The characterization of powders was performed by analysis of particle size and morphology, density, compressibility index determination, flow properties and determination of swelling profile. The tablets were evaluated according to their physical parameters of quality and to the in vitro release of nimesulide, as well as the analysis of the mechanisms of drug release by appropriate mathematical models. The set of results showed that the HPMC/Nimesulide mixture exhibited satisfactory physical characteristics (size, shape, density and flow). The release profile demonstrated an effective control upon drug release in enteric environment and presented more correlation with Korsmeyer-Peppas’ and Weibull’s mathematical models, indicating that the release of nimesulide occurs through the relaxation of the polymer chains

As políticas de ciência e tecnologia no Brasil: um estudo de seus desdobramentos para o desenvolvimento através da formação de mão-de-obra especializada

O presente trabalho discute a evolução histórica das políticas de ciência e tecnologia (C&T) no Brasil e seus desdobramentos para o desenvolvimento do país. É sabido que a C&T pode estimular o crescimento econômico, o investimento e a competitividade de um país. Nesse sentido, a inovação também se torna importante para as políticas de C&T. Além disso, o nível de pesquisa e desenvolvimento (P&D) acaba sendo estimulado, permitindo que haja dinamismo tecnológico e absorção de avanços – que são gerados em outros países – no país. No caso brasileiro, a C&T começou a se desenvolver ainda na época da ditadura (fim da década de 60), mas pode-se dizer que ela começou a ter uma importância realmente evidente a partir da década de 90 e, em particular, durante o governo Lula (2003-2010). Entende-se também que recursos humanos com alta qualificação constituem um contingente fundamental para o desenvolvimento econômico e social do país, sendo decisivo para a difusão e a criação de novos produtos e processos. É a partir destas idéias, que o trabalho discute a forma como as políticas de C&T voltadas para a formação de mãode- obra especializada podem ajudar no processo de desenvolvimento do país através, entre outros, de um maior esforço em P&D, de projetos cooperativos que unem as universidades e empresas e, conseqüentemente, através da promoção de inovações

A formação do professor reflexivo no periódico Educação e Pesquisa - 1984 a 2010

Este estudo teve como objetivo compreender a formação docente na perspectiva do professor reflexivo, buscando averiguar nesse processo temas emergentes vinculados ao periódico Educação e Pesquisa, fonte de coleta de dados. Neste itinerário optamos pela pesquisa qualitativa, do tipo descritivo, escolhendo como técnica para a coleta de dados a fonte documental, seguida de análise documental. Entre os resultados coletados na revista, periódico de 1975 a 2010, apontaram-se como significativos os eixos reflexão, saberes, identidade e profissionalização docente, prática pedagógica, formação inicial e formação continuada. Neste contexto observou-se que as pesquisas de ênfase critico reflexiva vem contribuindo teoricamente para a disseminação do conceito da formação reflexiva inicial e continuada de professores que buscam por meio desta perspectiva o desenvolvimento profissional e o reconhecimento como produtores de conhecimento na área que atuam. Outras questões observadas assinalaram para a compreensão da formação reflexiva como uma concepção que pode auxiliar o docente no processo de reconhecimento da sua identidade profissional marcada pela influência direta da cultura da qual os docentes estão inseridos. Nesta direção foi também identificado que as tomadas de decisões no âmbito escolar, dentro e fora da sala de aula, requerem do docente a mobilização de saberes que vão além da teoria aprendida nos anos de formação acadêmica, buscando auxilio nas vivências pré-profissionais para a elaboração dos saberes docentes. Porém, elucidou-se que os docentes reflexivos e investigadores da sua própria prática tendem a ser favorecidos por este tipo de prática, podendo provocar inovações na educação e em seu próprio desenvolvimento profissional. Concluindo, pode-se dizer que o estudo do periódico Educação e Pesquisa perspectivado... (Resumo completo, clicar acesso eletrônico abaixo)

Estudo de cerâmicas avançadas para fins de blindagem balística

The research involving new materials has always been considered as a differential in the development of a technology company. This occurred naturally since ancient times, often motivated by reasons of a certain age, where the most common material used was also the name of your time and may be cited as an example the Bronze Age, and later was the Iron. Currently, the use of firearms are they used in resolving conflicts between countries, or a more equivocal, as an instrument of social banditry make innovations in the area of shielding welcome, whether for personal use, in the form of vests or vehicle such as cars, tanks and even aircraft. In this context, is a Silicon Carbide Ceramic, with low density and high hardness. Thus, the aim of this study is the evaluation and comparison of these materials, seeking to improve their properties by means of additives such as boron and silicon metal and amorphous YAG. For this work, the specimens were pre-shaped by means of uniaxial later to be referred for isostatic pressing and sintering. The maximum percentage for each additive was 5%, except for the YAG whose percentage was 8.2% (mass percentage). All compositions were subjected to the same tests (x-ray diffraction, apparent density, optical microscopy, Vickers hardness, scanning electron Microscopita), so that one could draw a comparison between the materials under study, samples that showed better mechanical properties and micro structural, related here by hardness testing and microscopy (optical and SEM) were the silicon carbide doped with YAG and alumina samples, demonstrating the potential of these materials for ballistic protection. Other compositions have high porosity, which is highly undesirable, since in order to harmful influences on the mechanical properties discussed below

Células-Tronco e Terapia Celular: Suporte didático para o ensino fundamental e médio

Nos últimos anos, as pesquisas com células-tronco têm despertado um grande interesse nos meios de comunicação, levando a população a encarar esse novo conhecimento científico como sendo um método promissor para a cura de inúmeras doenças até agora, tidas como incuráveis. Deste modo, mostra-se fundamental que o ensino de Biologia se volte para o desenvolvimento de competências que permitam ao aluno entender, discutir, questionar, analisar, adquirir um posicionamento crítico diante das informações postas e fazendo uso dos conhecimentos adquiridos, servir como multiplicadores do conhecimento científico. Este trabalho tem como objetivo central a produção de um material didático contendo informações a respeito das células-tronco, quanto aos tipos, características, classificações, métodos de coleta e as terapias desenvolvidas no Brasil. Informações estas que servem como auxílio para professores de Ciências e Biologia, na compreensão e atualização perante as inovações científicas relacionadas às células-tronco. O capítulo 6 vem como uma sugestão de atividades possíveis a serem desenvolvidas a partir desse tema. Apresenta ainda um CD-ROM contendo uma síntese do assunto proposto a servir como suporte no preparo de aulas didáticas relacionadas ao tema

Arte & jóia: uma análise da joalheria contemporânea brasileira

Since the beginning human needs to produce elements artistics, in such way that can set their history and pass a language, be it as art, culture and / or as an identity. Within this context, the jewelry was being developed, its characteristics and aesthetics, in a period, being artistic, historic, marking this form a civilization, a document, with its innovations and concepts. Nowadays, with technology, trade and the need to search for creations and innovations in the jewelry section, was prepared and developed those that we call Art jewelry. This study brings a trajectory through the history of jewelry art and jewelry; specifying in the contemporary period the various types of jewelry such as author and industry, its artists and jewelers; parts exhibited in museums and designed for competitions, thus creating a analysis of contemporary jewelry