Diário da Constituinte [gravação de video] : [programa n. 069]

A Comissão da Organização dos Poderes e Sistema de Governo discute duas questões de ordem. Na primeira o deputado Amaral Netto (PDS-RJ)propõe a substituição dos relatores que tiveram os anteprojetos rejeitados e na segunda o deputado José Genoíno (PT-SP) quer mudar a composição da Comissão de Sistematização. A Comissão da Organização Eleitoral, Partidária e Garantia das Instituições discutiu duas questões que passaram por mais de uma subcomissão, e tiveram propostas diferentes, como o mandato presidencial, por exemplo. a Subcomissão do Poder executidvo proôs cinco anos, mas a Subcomissão do Sistema Eleitora sugeriu quatro. O deputados Paulo Delgado (PT-SP) diz que esta é uma questão política, e deve ser debatida com serenidade. Quarenta dias de trabalho sem interrupção, várias seções e audiências públicas e três viagens a regiões com conflito agrário, este o balanço dos trabalhos da Subcomissão da Política Agrícola e Fundiária e da Reforma Agrária. No resultado final houveram muitas divergências. O parecer do relator Oswaldo Lima Filho (PMDB-PE) foi preterido por um substitutivo, do deputado Rosa Prata (PMDB-MG), o que foi considerado irregular pelo Regimento Interno. Oswaldo Lima Filho (PMDB-PE) diz que o substitutivo é uma fraude. Já o deputado Rosa Prata (PMDB-MG) diz que é uma proposta menos radical e ajustada a realidade do país. Outro tema a ser discutido é o tamanho da propriedade rural. Os deputados Amaury Muller (PDT-RS) e Fernando Sant'Anna (PCB-BA) são a favor da limitação da propriedade. Ivo Vanderlinde (PMDB-SC) acredita no entendimento, para fazer da Reforma Agrária, uma realidade.

Case study: Video learning for engineering apprentices

Case study on level 2 and 3 engineering apprentices at PETA training and consultancy services.

Large Hadron Collider (LHC) large scale high volume data transport – end-to-end performance from the scientific application viewpoint

Presentation slides as part of the Janet network end-to-end performance initiative

eduPERT: a participants viewpoint, including some history and the current/future status

Supporting presentation slides from the Janet network end to end performance initiative workshop

Illustrated key to the genera of free-living marine nematodes of the Order Enoplida

A pictorial key to US genera of free-living marine nematodes in the order Enoplida is presented. Specific morphological and anatomical features are iUustrated to facilitate use of the key. The purpose of this work is to provide a single key to the genera of enoplid nematodes to facilitate identification of these organisms by nematologists and marine biologists working with meiofauna. (PDF file contains 32 pages.)

Detection of Atomic Scale Changes in the Free Volume Void Size of Three-Dimensional Colorectal Cancer Cell Culture Using Positron Annihilation Lifetime Spectroscopy

5 p.

Metal-Free Polymethyl Methacrylate (PMMA) Nanoparticles by Enamine "Click" Chemistry at Room Temperature

"Click" chemistry has become an efficient avenue to unimolecular polymeric nanoparticles through the self-crosslinking of individual polymer chains containing appropriate functional groups. Herein we report the synthesis of ultra-small (7 nm in size) polymethyl methacrylate (PMMA) nanoparticles (NPs) by the "metal-free" cross-linking of PMMA-precursor chains prepared by reversible addition-fragmentation chain transfer (RAFT) polymerization containing beta-ketoester functional groups. Intramolecular collapse was performed by the one-pot reaction of beta-ketoester moieties with alkyl diamines in tetrahydrofurane at r.t. (i.e., by enamine formation). The collapsing process was followed by size exclusion chromatography and by nuclear magnetic resonance spectroscopy. The size of the resulting PMMA-NPs was determined by dynamic light scattering. Enamine "click" chemistry increases the synthetic toolbox for the efficient synthesis of metal-free, ultra-small polymeric NPs.

Novel Papillomaviruses in Free-Ranging Iberian Bats: No Virus-Host Co-evolution, No Strict Host Specificity, and Hints for Recombination

Papillomaviruses (PVs) are widespread pathogens. However, the extent of PV infections in bats remains largely unknown. This work represents the first comprehensive study of PVs in Iberian bats. We identified four novel PVs in the mucosa of free-ranging Eptesicus serotinus (EserPV1, EserPV2, and EserPV3) and Rhinolophus ferrumequinum (RferPV1) individuals and analyzed their phylogenetic relationships within the viral family. We further assessed their prevalence in different populations of E. serotinus and its close relative E. isabellinus. Although it is frequent to read that PVs co-evolve with their host, that PVs are highly species-specific, and that PVs do not usually recombine, our results suggest otherwise. First, strict virus-host co-evolution is rejected by the existence of five, distantly related bat PV lineages and by the lack of congruence between bats and bat PVs phylogenies. Second, the ability of EserPV2 and EserPV3 to infect two different bat species (E. serotinus and E. isabellinus) argues against strict host specificity. Finally, the description of a second noncoding region in the RferPV1 genome reinforces the view of an increased susceptibility to recombination in the E2-L2 genomic region. These findings prompt the question of whether the prevailing paradigms regarding PVs evolution should be reconsidered.

Chemosensory response of marine flagellate towards L- and D- dissolved free amino acids generated during heavy grazing on bacteria

This study investigated the generation of dissolved free amino acids (DFAA) by the bacterivorous flagellate Rhynchomonas nasuta when feeding on abundant prey. Specifically, it examined whether this flagellate protist exhibits a chemosensory response towards those amino acids. The concentrations of glycine and the and D-enantiomers of glutamate, serine, threonine, alanine, and leucine were determined in co-cultures of the flagellate and bacteria. Glycine, L- and D-alanine, and L-serine were found to accumulate under these conditions in amounts that correlated positively with flagellate abundance, suggesting that protists are involved in their generation. Investigations of the chemotactic response of young and old foraging protists to the same amino acids, offered in concentrations similar to those previously generated, showed that glycine elicited the strongest attraction in both age groups. Young protists were strongly attracted to all the assayed amino acids, whereas older protists maintained a high level of attraction only for glycine. These results suggest that glycine generated by protists actively grazing in bacterially enriched patches functions as an infochemical, signaling to foraging protists the presence of available prey in the aquatic environment.