Role of glutathione in schizophrenia? The effect of brain glutathione depletion on prepulse inhibition (PPI) in rats and mice

Reductions in brain glutathione (GSH) levels have been reported in schizophrenia. We investigated the effects of brain GSH depletion on prepulse inhibition (PPI), a model of sensorimotor gating which is disrupted in individuals with schizophrenia. It was hypothesized that GSH depletion would lead to disruption of PPI similar to that seen in schizophrenia and enhance the effect of increased dopamine release by amphetamine. Sprague-Dawley rats and C57Bl/6 mice were treated with saline or 2-cyclohexene-1-one (CHX, 75 mg/kg and 120 mg/kg respectively) to deplete brain GSH. 225 minutes later the animals were injected with amphetamine (2.5 mg/kg in rats and 25 mg/kg in mice). Total brain GSH levels were measured using an enzymatic recycling assay. Surprisingly, in rats CHX treatment prevented the disruption of PPI by amphetamine. Thus, while there was the expected disruption of PPI caused by amphetamine on its own (average %PPI reduced from 58 ± 5 to 44 ± 4), in combination with CHX, amphetamine had no significant effect (67 ± 4 vs. 63 ± 3, respectively). In contrast to rats, in mice CHX had no effect on PPI. Thus, amphetamine similarly disrupted PPI after saline (41 ± 5 vs. 28 ± 5) and CHX pretreatment (45 ± 6 vs. 26 ± 5). There were significant 40-63% depletions of GSH in frontal cortex and striatum of CHX-treated rats and mice. These data show that GSH depletion in the brain by CHX treatment did not induce the expected decrease in PPI. Because the levels of GSH depletion in this study were similar to those found in schizophrenia, these results cast doubt on a direct interaction between brain GSH levels and PPI disruption in this illness. In rats, CHX treatment prevented the disruption of PPI caused by amphetamine. We have observed that resting levels of GSH are lower in rats than in mice. It is plausible that some oxidative damage may occur after amphetamine treatment alone, which induces marked release of the electroactive species, dopamine. In mice with their higher levels of GSH (either with or without CHX treatment) and in control rats, this does not cause functional effects. However, in CHX-treated rats GSH levels are reduced to a point where amphetamine-induced dopamine release may cause increased metabolism and lipid peroxidation inducing a decrease in postsynaptic dopamine receptor function and consequently leading to an apparent inhibition of the disruption of PPI. In conclusion, while individuals with schizophrenia show disruption of PPI and reduced brain GSH levels, in rats and mice brain GSH depletion alone does not impact on PPI. In combination with a hyperdopaminergic state, functional effects on PPI regulation were found. These effects warrant further investigation.

Changes in SWB following injury to different brain lobes

A neurological substrate for subjective wellbeing (SWB) has received little research attention.
Purpose This study was designed to conduct exploratory investigation into the neuroanatomical correlates of SWB, by monitoring the SWB of a head-injured population over a six-month period.
Method Seventy people with head injury (HI), aged 10–65, were studied. The SWB of each participant was measured, and computed tomography (CT) scans were analysed to obtain regional brain injury location (BIL).
Results SWB was associated with BIL. However, the hypothesis that individuals with left frontal injury would report lower SWB was not supported. Instead, it was observed that participants with injury to their right frontal lobe reported higher SWB than individuals with injury to other regions of the brain.
Conclusions This study provides initial exploration into the neuroanatomical correlates of SWB.

Natural convection inside dome shaped enclosures

In the present paper the analysis of heat transfer and free convective motion have been carried out numerically for dome shaped enclosures. The solution method is based on the finite element technique with the frontal solver and is used to examine the flow parameters and the heat transfer characteristics inside dome shaped enclosures of various offsets. In formulating the solution a general conic equation is considered to represent the dome of circular, elliptical, parabolic and hyperbolic shapes. The numerical results indicate that the circular and elliptical shapes of dome give higher heat transfer rate and offset of the dome effects convective heat transfer quite significantly. However, beyond 0.3 top dome offset, the change in overall heat transfer rate is not significant. In addition, the convective phenomenon influenced by a dome shaped cover results in establishing a secondary core region even at a moderate Rayleigh number when compared with an equivalent rectangular enclosure. A good comparison between the present numerical predictions and the previous published data is achieved.

Numerical investigation of natural convection inside complex enclosures

In this article, the analyses of heat transfer and free convective motion have been carried out numerically for various structures. The solution is based on a finite element method with the frontal solver to examine the flow parameters and heat transfer characteristics. Several dome configurations--such as flat, inclined, and dome shapes--are considered for the top of the enclosure. A general conic equation is considered to represent the dome as circular, elliptical, parabolic, or hyperbolic shape. The findings from this study indicate that the convective phenomenon is greatly influenced by the shape of the top cover dome and tends to form a secondary core even at a moderate Rayleigh number when compared with an equivalent rectangular enclosure. In addition, the circular and elliptical shapes of the dome give higher heat transfer rate. The effect of various "offset" of the dome and inclined roof on convective heat transfer is also found to be quite significant. However, beyond 0.3 of offset of the top cover for the dome and inclined roof, the change in overall heat transfer rate is minimal. The heat transfer coefficients of dome shaped and inclined roof enclosures are given and discussed.

Thunderstorm asthma

Thunderstorms have often been linked to epidemics of asthma, especially during the grass flowering season; however, the precise mechanisms explaining this phenomenon are unknown. Evidence of high respirable allergen loadings in the air associated with specific meteorologic events combined with an analysis of pollen physiology suggests that rupture of airborne pollen can occur. Strong downdrafts and dry, cold outflows distinguish thunderstorm rain from frontal rain. The weather system of a mature thunderstorm likely entrains grass pollen into the cloud base, where pollen rupture would be enhanced, then transports the respirablesized fragments of pollen debris to ground level where outflows distribute them ahead of the rain. The conditions occurring at the onset of a thunderstorm might expose susceptible people to a rapid increase in concentrations of pollen allergens in the air that can readily deposit in the lower airways and initiate asthmatic reactions.

π-Selective stationary phases: (II) Adsorption behaviour of substituted aromatic compounds on n-alkyl-phenyl stationary phases

The frontal analysis method was used to measure the adsorption isotherms of phenol, 4-chlorophenol, p-cresol, 4-methoxyphenol and caffeine on a series of columns packed with home-made alkyl-phenyl bonded silica particles. These ligands consist of a phenyl ring tethered to the silica support via a carbon chain of length ranging from 0 to 4 atoms. The adsorption isotherm models that fit best to the data account for solute–solute interactions that are likely caused by π–π interactions occurring between aromatic compounds and the phenyl group of the ligand. These interactions are the dominant factor responsible for the separation of low molecular weight aromatic compounds on these phenyl-type stationary phases. The saturation capacities depend on whether the spacer of the ligands have an even or an odd number of carbon atoms, with the even alkyl chain lengths having a greater saturation capacity than the odd alkyl chain lengths. The trends in the adsorption equilibrium constant are also significantly different for the even and the odd chain length ligands.

Tecnica simples de marcacao externa de reprodutores de tilapia nilotica (Oreochromis niloticus)

With the goal of organizing a control program of Nile tilapia (Oreochromis niloticus) reproduction in laboratory, a tecnique with ceramic pearls held on dorsal musculature by transfixion with flexible sintetic string was developed. Sixty fishes with 12g average weight and 60 with 19g average weight were used. Marking was done on three positions: frontal (F), median (M), and caudal (C). Different combinations of three collored pearls were fixed on the right side (defining number), and were linked to only one pearl in the left side (defining sex), with a slack of about 1.5cm to prevent growth damage. The animals were individually identified and weigthed at 30, 60 and 130 days after surgery. Results showed that both F and M positions allowed normal growth and reproductive behavior, whereas the C position induced mortality and loss of marker in some specimens. The M position is recomended as the best, or the intermediate between F and M positions.

"Tomorrow's car" : Deakin Universities new cross vehicle that combines the best of 2 worlds

Motorbike riders are 34-times more likely to die in a crash compared to car drivers per km travelled (1). Such safety risks together with special skill requirements for the driver and much lower comfort compared to normal cars are the main reasons why motorbikes represent only a fraction of all vehicle sales in developed countries. Deakin University is developing a revolutionary cross-over fun vehicle with ultra low fuel consumption and emissions. This new vehicle generation combines the best of two worlds: the fun to drive, low cost, and small size of a scooter together with the safety, comfort and easiness to operate of a car. The result is a vehicle that is more fuel efficient than most cars or even scooters.

Various tilting cross over vehicles have been presented over the last decade that were trying to automate the tilting control of narrow vehicles to make them safer. Examples of these concepts are the Carver, Clever and in some way also the MP3 scooter from Piaggio. The problem with fully enclosed concepts like the Carver or Clever is that they require very complex and therefore also expensive tilting control systems so that the vehicles are not price competitive compared to low cost micro cars or even normal small cars. The MP3 on the other hand comes with a tilting control system which is only semi automatic so that typical car advantages - comprehensive safety features like crush zones, roll over protection, air bags, safety belts or comfort features like full weather protection including heating and cooling – can not be provided.

Deakin’s approach is quite different to the above mentioned concepts. The requirements were derived based on two different investigations: The first step was a critical evaluation of social trends and the second step was an in-depth benchmarking study of existing concepts which identified the typical strengths and weaknesses of these concepts. In a critical next step a new concept was created that addresses most of the weaknesses of existing tilting three-wheelers in a holistic approach by setting clear priority rankings for the vehicle targets, based on current trends. The priorities were set in the following order: Safety, Affordability, Fun and Efficiency (SAFE).

The key feature that enables an enclosed tilting vehicle is a fully automatic tilting control system. With an automatic tilting control system the driver does not need to put the feet on the ground to balance the vehicle when he stops, so the vehicle can be built with a full enclosure. This allows the implementation of typical car like safety features (seat belts, roll over structure, crush zones, air bags). The SafeRide™ tilting control system is a passive system that involves the driver’s balancing sense in its feedback control system. The vehicle has typical scooter like steering characteristics, where the steering is initiated through countersteering. Another safety critical design feature is the crush zone between the two front wheels which is not possible with only one front wheel or with the powertrain positioned between the front wheels, as the powertrain can’t absorb a lot of energy due to its structural stiffness and density. The passive tilting control system is quite simple and therefore makes the vehicle very affordable, an important factor for successful commercialisation.

Another advantage of integrating the human balancing senses in the feedback control of the tilting system is that the system kicks in slightly after the human balancing reacts. In some instances that can generate the typical adrenalin thrill known from riding a bike. This fun factor is quite common with many trend sports like mountain biking, surfing, roller-skating, snowboarding, or skateboarding. Some of these sports have seen very rapid growth only a short time after they have been invented. Utilising the human balancing system during driving also makes the vehicle safer as the adrenalin is produced after reaching a semi-stable driving condition that is controlled by the vehicles tilting control system, but before the vehicle reaches an unstable driving condition that can not be controlled by the vehicle but only (eventually) by the driver – if he has got the required driving skill and if he is alert enough.

Efficiency superior to most cars and scooters is achieved by the aerodynamics of a fully enclosed body structure in combination with the small frontal area of a typical scooter and the droplet shape enabled by the relatively wide front with 2 wheels and the very narrow tail with only one rear wheel. The passive tilting system also contributes to the extreme efficiency as the system only draws some small electrical power for the electronic control unit. Another feature is a low cost exhaust energy recovery system which is discussed in another paper.

Illumination-invariant face recognition from a single image across extreme pose using a dual dimension AAM ensemble in the thermal infrared spectrum

Over the course of the last decade, infrared (IR) and particularly thermal IR imaging based face recognition has emerged as a promising complement to conventional, visible spectrum based approaches which continue to struggle when applied in practice. While inherently insensitive to visible spectrum illumination changes, IR data introduces specific challenges of its own, most notably sensitivity to factors which affect facial heat emission patterns, e.g. emotional state, ambient temperature, and alcohol intake. In addition, facial expression and pose changes are more difficult to correct in IR images because they are less rich in high frequency detail which is an important cue for fitting any deformable model. In this paper we describe a novel method which addresses these major challenges. Specifically, when comparing two thermal IR images of faces, we mutually normalize their poses and facial expressions by using an active appearance model (AAM) to generate synthetic images of the two faces with a neutral facial expression and in the same view (the average of the two input views). This is achieved by piecewise affine warping which follows AAM fitting. A major contribution of our work is the use of an AAM ensemble in which each AAM is specialized to a particular range of poses and a particular region of the thermal IR face space. Combined with the contributions from our previous work which addressed the problem of reliable AAM fitting in the thermal IR spectrum, and the development of a person-specific representation robust to transient changes in the pattern of facial temperature emissions, the proposed ensemble framework accurately matches faces across the full range of yaw from frontal to profile, even in the presence of scale variation (e.g. due to the varying distance of a subject from the camera). The effectiveness of the proposed approach is demonstrated on the largest public database of thermal IR images of faces and a newly acquired data set of thermal IR motion videos. Our approach achieved perfect recognition performance on both data sets, significantly outperforming the current state of the art methods even when they are trained with multiple images spanning a range of head views.

Vesselness features and the inverse compositional AAM for robust face recognition using thermal IR

Over the course of the last decade, infrared (IR) and particularly thermal IR imaging based face recognition has emerged as a promising complement to conventional, visible spectrum based approaches which continue to struggle when applied in the real world. While inherently insensitive to visible spectrum illumination changes, IR images introduce specific challenges of their own, most notably sensitivity to factors which affect facial heat emission patterns, e.g. emotional state, ambient temperature, and alcohol intake. In addition, facial expression and pose changes are more difficult to correct in IR images because they are less rich in high frequency detail which is an important cue for fitting any deformable model. In this paper we describe a novel method which addresses these major challenges. Specifically, to normalize for pose and facial expression changes we generate a synthetic frontal image of a face in a canonical, neutral facial expression from an image of the face in an arbitrary pose and facial expression. This is achieved by piecewise affine warping which follows active appearance model (AAM) fitting. This is the first publication which explores the use of an AAM on thermal IR images; we propose a pre-processing step which enhances detail in thermal images, making AAM convergence faster and more accurate. To overcome the problem of thermal IR image sensitivity to the exact pattern of facial temperature emissions we describe a representation based on reliable anatomical features. In contrast to previous approaches, our representation is not binary; rather, our method accounts for the reliability of the extracted features. This makes the proposed representation much more robust both to pose and scale changes. The effectiveness of the proposed approach is demonstrated on the largest public database of thermal IR images of faces on which it achieved 100% identification rate, significantly outperforming previously described methods

Zooplankton abundance and community structure over a 4000 km transect in the North-east Atlantic

Zooplankton samples were collected by a high speed sampler, the U-Tow, in the north-east Atlantic between 61.6 and 36.7°N during June and July 1996, and were used to examine the causality of spatial distributions along a 4000 km transect. Peak zooplankton abundance and biovolume estimations were associated with a frontal system at 48–52°N, which separated hydrographically distinct water masses. The zooplankton assemblage was dominated by herbivorous/omnivorous taxa in the northern regions, and by carnivorous taxa in the southern regions. Arguments are developed to suggest that the switch in both the zooplankton size structure and trophic status, centred within the frontal region, are consistent with ‘bottom-up’ control of zooplankton size structure in this region of the Atlantic.

Automatic face recognition for film character retrieval in feature-length films

The objective of this work is to recognize all the frontal faces of a character in the closed world of a movie or situation comedy, given a small number of query faces. This is challenging because faces in a feature-length film are relatively uncontrolled with a wide variability of scale, pose, illumination, and expressions, and also may be partially occluded. We develop a recognition method based on a cascade of processing steps that normalize for the effects of the changing imaging environment. In particular there are three areas of novelty: (i) we suppress the background surrounding the face, enabling the maximum area of the face to be retained for recognition rather than a subset; (ii) we include a pose refinement step to optimize the registration between the test image and face exemplar; and (iii) we use robust distance to a sub-space to allow for partial occlusion and expression change. The method is applied and evaluated on several feature length films. It is demonstrated that high recall rates (over 92%) can be achieved whilst maintaining good precision (over 93%).

Procedural learning deficits in specific language impairment (SLI): a meta-analysis of serial reaction time task performance

Meta-analysis and meta-regression were used to evaluate whether evidence to date demonstrates deficits in procedural memory in individuals with specific language impairment (SLI), and to examine reasons for inconsistencies of findings across studies. The Procedural Deficit Hypothesis (PDH) proposes that SLI is largely explained by abnormal functioning of the frontal-basal ganglia circuits that support procedural memory. It has also been suggested that declarative memory can compensate for at least some of the problems observed in individuals with SLI. A number of studies have used Serial Reaction Time (SRT) tasks to investigate procedural learning in SLI. In this report, results from eight studies that collectively examined 186 participants with SLI and 203 typically-developing peers were submitted to a meta-analysis. The average mean effect size was .328 (CI95: .071, .584) and was significant. This suggests SLI is associated with impairments of procedural learning as measured by the SRT task. Differences among individual study effect sizes, examined with meta-regression, indicated that smaller effect sizes were found in studies with older participants, and in studies that had a larger number of trials on the SRT task. The contributions of age and SRT task characteristics to learning are discussed with respect to impaired and compensatory neural mechanisms in SLI.

Multitasking: multiple, domain-specific cognitive functions in a virtual environment

Multitasking among three or more different tasks is a ubiquitous requirement of everyday cognition, yet rarely is it addressed in research on healthy adults who have had no specific training in multitasking skills. Participants completed a set of diverse subtasks within a simulated shopping mall and office environment, the Edinburgh Virtual Errands Test (EVET). The aim was to investigate how different cognitive functions, such as planning, retrospective and prospective memory, and visuospatial and verbal working memory, contribute to everyday multitasking. Subtasks were chosen to be diverse, and predictions were derived from a statistical model of everyday multitasking impairments associated with frontal-lobe lesions (Burgess, Veitch, de Lacy Costello, & Shallice, 2000b). Multiple regression indicated significant independent contributions from measures of retrospective memory, visuospatial working memory, and online planning, but not from independent measures of prospective memory or verbal working memory. Structural equation modelling showed that the best fit to the data arose from three underlying constructs, with Memory and Planning having a weak link, but with both having a strong directional pathway to an Intent construct that reflected implementation of intentions. Participants who followed their preprepared plan achieved higher scores than those who altered their plan during multitask performance. This was true regardless of whether the plan was efficient or poor. These results substantially develop and extend the Burgess et al. (2000b) model to healthy adults and yield new insight into the poorly understood area of everyday multitasking. The findings also point to the utility of using virtual environments for investigating this form of complex human cognition.