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OBJETIVO: Desenvolver um questionrio de atividade fsica para adolescentes brasileiros e verificar sua validade e reprodutibilidade. MTODOS: Participaram do estudo 94 adolescentes (30 meninos e 64 meninas) com idade entre 11 a 16 anos, em 2004. O questionrio foi composto por 17 questes sobre atividades habituais exercidas nos ltimos 12 meses (exerccios fsicos/esportes e atividades de locomoo) e foi padronizado para gerar escores semanal e anual. Como mtodo de referncia utilizou-se o teste de corrida vai-e-vem de 20 metros com as variveis tempo em minutos, velocidade mxima em km/h, consumo mximo de oxignio e freqncia cardaca mxima. Para a anlise de validao, foram utilizados o coeficiente de Spearman e correlao ajustada por idade. Para a anlise da reprodutibilidade, utilizou-se medida repetida com intervalo de 15 dias e coeficiente de correlao intraclasse. RESULTADOS: Para o escore semanal de atividade fsica, os maiores coeficientes de correlao foram obtidos com o tempo total para a anlise em conjunto (r=0,19), velocidade total para os meninos (r=0,20), e consumo mximo de oxignio e tempo total para as meninas (r=0,17). Para o escore anual de atividade fsica, os maiores coeficientes de correlao foram obtidos com o tempo total para a anlise em conjunto (r=0,30), freqncia cardaca final aps o ajuste pela idade para os meninos (r=0,22) e tempo total para as meninas (r=0,23). Nas anlises de reprodutibilidade, a correlao do escore semanal foi de 0,61 e a do escore anual foi de 0,68. CONCLUSES: O questionrio apresentou evidncias de validade e reprodutibilidade. Recomenda-se sua utilizao para avaliao da atividade fsica habitual em estudos epidemiolgicos com adolescentes.

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OBJETIVO: Estratgias metodolgicas vm sendo desenvolvidas para minimizar o efeito do erro de medida da dieta. O objetivo do estudo foi descrever a aplicao de uma estratgia para correo da informao diettica pelo erro de medida. MTODOS: Foram obtidos dados de consumo alimentar pela aplicao do Questionrio de Freqncia Alimentar a 79 adolescentes do Municpio de So Paulo em 1999. Os dados dietticos obtidos foram corrigidos por meio de regresso linear, aps o ajuste pela energia usando-se o mtodo dos resduos. O mtodo de referncia utilizado foi o recordatrio de 24 horas, aplicado em trs momentos distintos. RESULTADOS: Os valores corrigidos aproximaram-se dos valores de referncia. O fator de correo lambda foi de 0,89 para energia. Para os macronutrientes, os fatores foram de 0,41; 0,22 e 0,20, para carboidratos, lipdios e protenas, respectivamente. CONCLUSES: As mdias e desvios-padro dos valores corrigidos denotam que houve uma correo do erro de medida. Apesar disso, debate-se o desempenho desses mtodos, que so notoriamente imperfeitos quando seus pressupostos tericos no so atendidos, o que comum nos estudos da dieta que usam instrumentos de medida baseados no relato dos indivduos.

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Background - The rate and fitness effects of mutations are key in understanding the evolution of every species. Traditionally, these parameters are estimated in mutation accumulation experiments where replicate lines are propagated in conditions that allow mutations to randomly accumulate without the purging effect of natural selection. These experiments have been performed with many model organisms but we still lack empirical estimates of the rate and effects of mutation in the protists. Results - We performed a mutation accumulation (MA) experiment in Tetrahymena thermophila, a species that can reproduce sexually and asexually in nature, and measured both the mean decline and variance increase in fitness of 20 lines. The results obtained with T. thermophila were compared with T. pyriformis that is an obligate asexual species. We show that MA lines of T. thermophila go to extinction at a rate of 1.25 clonal extinctions per bottleneck. In contrast, populations of T. pyriformis show a much higher resistance to extinction. Variation in gene copy number is likely to be a key factor in explaining these results, and indeed we show that T. pyriformis has a higher mean copy number per cell than T. thermophila. From fitness measurements during the MA experiment, we infer a rate of mutation to copy number variation of 0.0333 per haploid MAC genome of T. thermophila and a mean effect against copy number variation of 0.16. A strong effect of population size in the rate of fitness decline was also found, consistent with the increased power of natural selection. Conclusions - The rate of clonal extinction measured for T. thermophila is characteristic of a mutational degradation and suggests that this species must undergo sexual reproduction to avoid the deleterious effects detected in the laboratory experiments. We also suggest that an increase in chromosomal copy number associated with the phenotypic assortment of amitotic divisions can provide an alternative mechanism to escape the deleterious effect of random chromosomal copy number variation in species like T. pyriformis that lack the resetting mechanism of sexual reproduction. Our results are relevant to the understanding of cell line longevity and senescence in ciliates.

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The fatty acid profile of erythrocyte membranes has been considered a good biomarker for several pathologic situations. Dietary intake, digestion, absorption, metabolism, storage and exchange amongst compartments, greatly influence the fatty acids composition of different cells and tissues. Lipoprotein and hepatic lipases were also involved in fatty acid availability. In the present work we examined the correlations between fatty acid in Red Blood Cells (RBCs) membranes, the fatty acid desaturase and elongase activities, glycaemia, blood lipids, lipoproteins and apoproteins, and the endothelial lipase (EL) mass in plasma. Twenty one individuals were considered in the present study, with age >18 y. RBCs membranes were obtained and analysed for fatty acid composition by gas chromatography. The amount of fatty acids (as percentage) were analysed, and the ratios between fatty acid 16:1/16:0; 18:1/18:0; 18:0/16:0; 22:6 n-3/20:5 n-3 and 20:4 n-6/18:2 n-6 were calculated. Bivariate analysis (rs) and partial correlations were determined. SCD16 estimation activity correlated positively with BMI (rs=0.466, p=0.043) and triacylglycerols (TAG) (rs=0.483, p=0.026), and negatively with the ratio ApoA1/ApoB (rs=-0.566, p=0.007). Endothelial lipase (EL) correlated positively with the EPA/AA ratio in RBCs membranes (rs=0.524, p=0.045). After multi-adjustment for BMI, age, hs-CRP and dietary n3/n6 ratio, the correlations remained significant between EL and EPA/AA ratio. At the best of our knowledge this is the first report that correlated EL with the fatty acid profile of RBCs plasma membranes. The association found here can suggest that the enzyme may be involved in the bioavailability and distribution of n-3/n-6 fatty acids, suggesting a major role for EL in the pathophysiological mechanisms involving biomembranes fatty acids, such as in inflammatory response and eicosanoids metabolites pathways.

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A Indstria Txtil do Ave S.A. (ITA) dedica-se, desde 1948, produo de componentes txteis para pneus em forma de fio torcido (corda) e tela. Estes componentes so quimicamente activados e impregnados em estufas, possibilitando assim a posterior adeso ao pneu. A mquina de impregnar corda Single End composta pelos grupos de estiragem, por um recipiente contendo a soluo qumica e por 4 estufas em srie. A mquina de impregnar tela Zell composta pelos grupos de estiragem, pelos acumuladores de sada e entrada, pelos recipientes com as solues qumicas e por um grupo de 7 estufas em srie. O aquecimento das estufas feito atravs da queima de gs natural. O presente trabalho teve como objectivo a realizao de uma auditoria energtica ITA com um especial destaque s mquinas de impregnar corda (Single End) e tela (Zell). As correntes de entrada que contribuem para a potncia trmica de impregnao so a combusto do gs natural, o ar de combusto, o ar fresco, o artigo em verde e as solues qumicas. As correntes de sada correspondem aos gases de combusto e exausto, ao artigo impregnado e s perdas trmicas. A auditoria mquina Single End mostrou que a potncia trmica de impregnao de 413,1 kW. Dessa potncia trmica, 77,2% correspondem combusto do gs natural, 6,7% ao ar de combusto, 15% ao ar fresco, 0,7% s cordas em verde e 0,4% soluo qumica. Da potncia trmica de sada, 88,4% correspondem aos gases de combusto e exausto, 3,2% s cordas impregnadas e 8,4% s perdas trmicas. Da auditoria mquina Zell observou-se que a potncia trmica de impregnao de 5630,7 kW. Dessa potncia, 73,3% corresponde combusto do gs natural, 1,6% ao ar de combusto, 24,5% ao ar fresco, 0,3% tela em verde e 0,3% s solues qumicas. Da potncia trmica de sada, 65,2% correspondem aos gases de combusto e exausto, 3,1% tela impregnada e 31,7% s perdas trmicas. Foram sugeridas como medidas de optimizao a reduo dos caudais de exausto das estufas e o aumento de temperatura do ar fresco. O aumento da temperatura do ar fresco da mquina de impregnar Single End para 50 C, usando ar quente dos torcedores, leva a uma poupana de 0,22 /h, com um perodo de retorno do investimento de 13 anos e 4 meses enquanto o aumento para 120 C, usando o calor dos gases de combusto e exausto, reduz os custos em 0,88 /h, sendo o perodo de retorno para esse investimento de 2 anos e 6 meses. Na mquina de impregnar Zell, uma reduo de 15% no caudal de exausto numa das estufas leva a ganhos de 3,43 /h. O aumento de temperatura do ar fresco para 45 C, usando o calor de gases de combusto e exausto, leva a uma poupana de 9,93 /h sendo o perodo de retorno para cada uma das duas sugestes de investimento de 5 meses e 9 meses.

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OBJECTIVE: To analyze the scoring obtained by an instrument, which evaluates the ability to read and understand items in the health care setting, according to education and age. METHODS: The short version of the Test of Functional Health Literacy in Adults was administered to 312 healthy participants of different ages and years of schooling. The study was conducted between 2006 and 2007, in the city of So Paulo, Southeastern Brazil. The test includes actual materials such as pill bottles and appointment slips and measures reading comprehension, assessing the ability to read and correctly pronounce a list of words and understand both prose passages and numerical information. Pearson partial correlations and a multiple regression model were used to verify the association between its scores and education and age. RESULTS: The mean age of the sample was 47.3 years(sd=16.8) and the mean education was 9.7 years(sd=5; range: 1 - 17). A total of 32.4% of the sample showed literacy/numeracy deficits, scoring in the inadequate and marginal functional health literacy ranges. Among the elderly (65 years or older) this rate increased to 51.6%. There was a positive correlation between schooling and scores (r=0.74; p<0.01) and a negative correlation between age and the scores (r=-0.259; p<0.01). The correlation between the scores and age was not significant when the effects of education were held constant (rp=-0.031, p=0.584). A significant association (B=3.877, Beta =0.733; p<0.001) was found between schooling and scores. Age was not a significant predictor in this model (B=-0.035, Beta=-0.22; p=0.584). CONCLUSIONS: The short version of the Test of Functional Health Literacy in Adults was a suitable tool to assess health literacy in the study population. The high number of individuals classified as functional illiterates in this test highlights the importance of special assistance to help them properly understand directions for healthcare.

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OBJECTIVE: To identify clustering areas of infants exposed to HIV during pregnancy and their association with indicators of primary care coverage and socioeconomic condition. METHODS: Ecological study where the unit of analysis was primary care coverage areas in the city of Porto Alegre, Southern Brazil, in 2003. Geographical Information System and spatial analysis tools were used to describe indicators of primary care coverage areas and socioeconomic condition, and estimate the prevalence of liveborn infants exposed to HIV during pregnancy and delivery. Data was obtained from Brazilian national databases. The association between different indicators was assessed using Spearman's nonparametric test. RESULTS: There was found an association between HIV infection and high birth rates (r=0.22, p<0.01) and lack of prenatal care (r=0.15, p<0.05). The highest HIV infection rates were seen in areas with poor socioeconomic conditions and difficult access to health services (r=0.28, p<0.01). The association found between higher rate of prenatal care among HIV-infected women and adequate immunization coverage (r=0.35, p<0.01) indicates that early detection of HIV infection is effective in those areas with better primary care services. CONCLUSIONS: Urban poverty is a strong determinant of mother-to-child HIV transmission but this trend can be fought with health surveillance at the primary care level.

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Dissertao de Mestrado, Estudos Integrados dos Oceanos, 26 Fevereiro de 2016, Universidade dos Aores.

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OBJECTIVE: To assess the relationship between locus of control and knowledge, attitude and practice regarding pill and condom use among university students. METHODS: The inquiry was developed in Campinas, a city in Southeastern Brazil, in 2006. A total of 295 adolescent newcomers to a public university answered a structured questionnaire and Levenson's multidimensional locus of control scale. The scores of the dimensions of locus of control were calculated and Spearman's correlation coefficient was used to assess their correlation with knowledge and practice concerning pill and condom use. In order to assess the relationship between the dimensions of locus of control and sociodemographic variables and variables related to the individuals' sex life, Kruskal-Wallis and Mann-Whitney tests were used. RESULTS: Male adolescents had higher scores of powerful others externality when compared to female adolescents (p=0.01). Students living alone had lower internality (p=0.01). When locus of control was compared to condom use in the first intercourse, considering only the 102 students who informed the age of the beginning of sexual activity, greater internality was found among male adolescents who did not use condoms (p<0.05). When the locus of control scores were correlated with contraceptive knowledge and practice, it was found that the higher the powerful others externality locus, the lower the adequate use of contraceptive methods (r = -0.22, p=0.03). CONCLUSIONS: The powerful others externality locus influences the practice of contraceptive use in this group of adolescents.

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OBJECTIVE: To examine whether the low birth weight (LBW) paradox exists in Brazil. METHODS: LBW and cesarean section rates between 1995 and 2007 were estimated based on data from SINASC (Brazilian Live Births Database). Infant mortality rates (IMRs) were obtained using an indirect method that correct for underreporting. Schooling information was obtained from census data. Trends in LBW rate were assessed using joinpoint regression models. The correlations between LBW rate and other indicators were graphically assessed by lowess regression and tested using Spearman's rank correlation. RESULTS: In Brazil, LBW rate trends were non-linear and non-significant: the rate dropped from 7.9% in 1995 to 7.7% in 2000, then increased to 8.2% in 2003 and remained nearly steady thereafter at 8.2% in 2007. However, trends varied among Brazilian regions: there were significant increases in the North from 1999 to 2003 (2.7% per year), and in the South (1.0% per year) and Central-West regions (0.6% per year) from 1995 to 2007. For the entire period studied, higher LBW and lower IMRs were seen in more developed compared to less developed regions. In Brazilian States, in 2005, the higher the IMR rate, the lower the LBW rate (p=0.009); the lower the low schooling rate, the lower the LBW rate (p=0.007); the higher the number of neonatal intensive care beds per 1,000 live births, the higher the LBW rate (p=0.036). CONCLUSIONS: The low birth weight paradox was seen in Brazil. LBW rate is increasing in some Brazilian regions. Regional differences in LBW rate seem to be more associated to availability of perinatal care services than underlying social conditions.

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OBJETIVO: Descrever caractersticas sociodemogrficas e analisar a tendncia temporal da mortalidade de motociclistas traumatizados em acidentes de transporte. MTODOS: Estudo de sries temporais com dados de 580 bitos de motociclistas do Distrito Federal, de 1996 a 2007, obtidos do Sistema de Informaes sobre Mortalidade. Foram calculadas as taxas de mortalidade especficas segundo idade e sexo, as taxas padronizadas (mtodo direto) e a razo de bitos por frota (motocicletas). A mdia mvel centralizada da taxa padronizada de mortalidade de homens foi calculada para o perodo de trs anos e um modelo de regresso linear foi construdo para estudar a evoluo temporal da mortalidade. Para calcular o incremento anual da taxa de mortalidade padronizada utilizou-se o mtodo joinpoint (ponto de inflexo). RESULTADOS: A maior parte dos motociclistas mortos era do sexo masculino, (94,3%), pardo (71,0%) e tinha entre 20 e 39 anos (73,8%). A taxa padronizada de mortalidade de motociclistas (homens) residentes foi de 1,9 para 7,2 bitos/100 mil homens entre 1996 e 2007. Entre 1998 e 2007, a razo de bitos por frota passou de 2,0 bitos/10 mil motocicletas para 10,0 bitos/10 mil motocicletas entre os homens. Estimou-se incremento anual de 0,48 bito/100 mil homens (IC95% 0,31;0,65; p < 0,001). O incremento percentual anual da taxa padronizada de mortalidade para o sexo masculino foi de 36,2% no perodo 1998-2007 (IC 95% 21,2%;53,2%; p < 0,05). CONCLUSES: A taxa de mortalidade de motociclistas decorrente de acidentes de transporte aumentou expressivamente. Esse aumento explicado apenas em parte pelo aumento da frota de motocicletas. Caractersticas individuais dos condutores, bem como as condies locais do trfego, necessitam ser investigadas para o planejamento de polticas preventivas.

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OBJETIVO: Analizar la asociacin entre el rea geogrfica de procedencia en el uso de las citologas y la mamografa. MTODOS: Los datos analizados proceden Encuesta Nacional de Salud de Espaa-2006 dirigida a poblacin mayor de 16 aos. La Encuesta incluye 13.422 mujeres. Las variables dependientes fueron realizacin de una mamografa y de una citologa vaginal, ambos en los ltimos 12 meses. La medida de asociacin fue el odds ratio con intervalo de confianza al 95% calculado por regresin logstica. RESULTADOS: Tomando como referencia la poblacin espaola, la probabilidad de realizarse una mamografas entre las mujeres procedentes de frica fue 0,36 (IC95% 0,21;0,62) veces menor; Europa del Este 0,40 (IC95% 0,22;0,74) veces menor; Europa Occidental, EEUU y Canad, 0,60 (IC95% 0,43; 0,84) veces menor y Amrica Central / Sur 0,64 (IC95% 0,52;0,81) veces menor. En relacin a la prevencin de cncer de cervix, probabilidad de realizarse una citologa entre las mujeres Europa del Este fue 0,38 (IC95% 0,28;0,50) veces menor que la poblacin espaola, frica 0,47 (IC95%:0,33;0,67) veces menor y Europa Occidental, EEUU y Canad 0,61 (IC95% 0,46;0,81) veces menor. Dichas asociaciones fueron independientes de la edad, indicadores socioeconmicos, estado de salud y cobertura sanitaria. CONCLUSIONES: Las mujeres inmigrantes hacen menor uso de los programas de cribado que las mujeres autctonas. Este dato podra reflejar dificultades de acceso a los programas preventivos.

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The concentrations of 18 polycyclic aromatic hydrocarbons (PAHs) were determined in five commercially valuable squid species from different geographical origins (Atlantic, Indic and Pacific Oceans). Out of the 18 quantified PAHs (the 16 PAHs considered by US EPA as priority pollutants, dibenzo(a,l)pyrene and benzo(j)fluoranthene) only dibenz(a,h)anthracene was not detected. The total concentrations of PAHs varied by a factor of more than 100-fold, from 0.22 (Loligo gahi) to 60.9 lg/kg ww (Loligo reynaudii). Intraand inter-specific variability of PAH levels was statistically assessed. Nine carcinogenic (probable/possible) PAHs accounted for 1% (L. reynaudii) to 26% (Loligo opalescens) of the total PAHs content being the main contributors naphthalene (in Loligo duvaucelii, L. reynaudii and Loligo vulgaris species), chrysene (in L. opalescens) and indeno(1,2,3-cd)pyrene (in L. gahi). PAHs source analysis indicated that four of the five zones of capture of the different squid species are significantly affected by both petrogenic and pyrolytic sources. Assessment of the target carcinogenic risks, established by the US EPA, suggested that L. gahi (Atlantic Ocean) and L. opalescens (from Pacific Ocean) may pose additional risks for consumers, if not eaten in moderation, derived from benzo(a)pyrene ingestion.

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OBJECTIVE: To assess the effect of a health promotion program on cardiometabolic risk profile in Japanese-Brazilians. METHODS: A total of 466 subjects from a study on diabetes prevalence conducted in the city of Bauru, southeastern Brazil, in 2000 completed a 1-year intervention program (2005-2006) based on healthy diet counseling and physical activity. Changes in blood pressure and metabolic parameters in the 2005-2006 period were compared with annual changes in these same variables in the 2000-2005 period. RESULTS: During the intervention, there were greater annual reductions in mean (SD) waist circumference [-0.5(3.8) vs. 1.2(1.2) cm per year, p<0.001], systolic blood pressure [-4.6(17.9) vs. 1.8(4.3) mmHg per year, p<0.001], 2-hour plasma glucose [-1.2(2.1) vs. -0.2(0.6) mmol/L per year, p<0.001], LDL-cholesterol [-0.3(0.9) vs. -0.1(0.2) mmol/L per year, p<0.001] and Framingham coronary heart disease risk score [-0.25(3.03) vs. 0.11(0.66) per year, p=0.02] but not in triglycerides [0.2(1.6) vs. 0.1(0.42) mmol/L per year, p<0.001], and fasting insulin level [1.2(5.8) vs. -0.7(2.2) IU/mL per year, p<0.001] compared with the pre-intervention period. Significant reductions in the prevalence of impaired fasting glucose/impaired glucose tolerance and diabetes were seen during the intervention (from 58.4% to 35.4%, p<0.001; and from 30.1% to 21.7%, p= 0.004, respectively). CONCLUSIONS: A one-year community-based health promotion program brings cardiometabolic benefits in a high-risk population of Japanese-Brazilians.

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OBJETIVO: Estimar a prevalncia de casos sugestivos de LER/DORT e fatores associados trs dcadas aps a reestruturao bancria. MTODOS: Estudo transversal com 356 funcionrios de 27 agncias bancrias das redes pblica e privada de Porto Alegre, RS, entre abril e agosto de 2009. Foi utilizada anlise estatstica bruta e ajustada pelo modelo de Regresso de Poisson com varincia robusta, conduzida por modelo hierrquico em trs nveis, incorporando-se a estrutura do delineamento e ajuste para os conglomerados. Os resultados foram estratificados por porte da agncia e dicotomizados (&#8805; 25 e < 25 funcionrios). RESULTADOS: A prevalncia de casos sugestivos de LER/DORT foi menor nos homens (RP = 0,62 IC95% 0,47;0,81). Trabalhadores com idade entre 26 e 45 anos (RP = 2,51 IC95% 1,02;6,14) apresentaram maior prevalncia do desfecho. Indivduos com ps-graduao (RP = 0,45 IC95% 0,22;0,90) e tempo na funo entre 5,1 e 15 anos (RP = 0,62 IC95% 0,47;0,81) apresentaram fator de proteo para os casos sugestivos de LER/DORT. Ao estratificar as anlises por porte, nas agncias com 25 funcionrios ou menos, idade, renda e tempo na funo permaneceram associados, enquanto nas agncias com mais de 25 funcionrios, sexo e escolaridade associaram-se ao desfecho. CONCLUSES: Aspectos importantes no adoecimento por LER/DORT entre bancrios parecem hoje ser diferentes dos historicamente conhecidos. Ateno maior organizao do trabalho e s estratgias de gesto deveria ser considerada na elaborao de programas de preveno de adoecimento no trabalho bancrio.