The natural absence of RPA1N domain did not impair Leishmania amazonensis RPA-1 participation in DNA damage response and telomere protection.

We have previously shown that the subunit 1 of Leishmania amazonensis RPA (LaRPA-1) alone binds the G-rich telomeric strand and is structurally different from other RPA-1. It is analogous to telomere end-binding proteins described in model eukaryotes whose homologues were not identified in the protozoan's genome. Here we show that LaRPA-1 is involved with damage response and telomere protection although it lacks the RPA1N domain involved with the binding with multiple checkpoint proteins. We induced DNA double-strand breaks (DSBs) in Leishmania using phleomycin. Damage was confirmed by TUNEL-positive nuclei and triggered a G1/S cell cycle arrest that was accompanied by nuclear accumulation of LaRPA-1 and RAD51 in the S phase of hydroxyurea-synchronized parasites. DSBs also increased the levels of RAD51 in non-synchronized parasites and of LaRPA-1 and RAD51 in the S phase of synchronized cells. More LaRPA-1 appeared immunoprecipitating telomeres in vivo and associated in a complex containing RAD51, although this interaction needs more investigation. RAD51 apparently co-localized with few telomeric clusters but it did not immunoprecipitate telomeric DNA. These findings suggest that LaRPA-1 and RAD51 work together in response to DNA DSBs and at telomeres, upon damage, LaRPA-1 works probably to prevent loss of single-stranded DNA and to assume a capping function.

Classification of masses in mammographic image using wavelet domain features and polynomial classifier

Breast cancer is the most common cancer among women. In CAD systems, several studies have investigated the use of wavelet transform as a multiresolution analysis tool for texture analysis and could be interpreted as inputs to a classifier. In classification, polynomial classifier has been used due to the advantages of providing only one model for optimal separation of classes and to consider this as the solution of the problem. In this paper, a system is proposed for texture analysis and classification of lesions in mammographic images. Multiresolution analysis features were extracted from the region of interest of a given image. These features were computed based on three different wavelet functions, Daubechies 8, Symlet 8 and bi-orthogonal 3.7. For classification, we used the polynomial classification algorithm to define the mammogram images as normal or abnormal. We also made a comparison with other artificial intelligence algorithms (Decision Tree, SVM, K-NN). A Receiver Operating Characteristics (ROC) curve is used to evaluate the performance of the proposed system. Our system is evaluated using 360 digitized mammograms from DDSM database and the result shows that the algorithm has an area under the ROC curve Az of 0.98 ± 0.03. The performance of the polynomial classifier has proved to be better in comparison to other classification algorithms. © 2013 Elsevier Ltd. All rights reserved.

A transmission line model developed directly in phase domain

The phases of a transmission line are tightly coupled due to mutual impedances and admittances of the line. One way to accomplish the calculations of currents and voltages in multi phase lines consists in representing them in modal domain, where its n coupled phases are represented by their n propagation modes. The separation line in their modes of propagation is through the use of a modal transformation matrix whose columns are eigenvectors associated with the parameters of the line. Usually, this matrix is achieved through numerical methods which do not allow the achievement of an analytical model for line developed directly in the phases domain. This work will show an analytical model for phase currents and voltages of the line and results it will be applied to a hypothetical two-phase. It will be shown results obtained with that will be compared to results obtained using a classical model © 2003-2012 IEEE.

A distributed-parameters transmission line model developed directly in the phase domain

This article shows a transmission line model developed directly in the phase domain. The proposed model is based on the relationships between the phase currents and voltages at both the sending and receiving ends of a single-phase line. These relationships, established using an ABCD matrix, were extended to multi-phase lines. The proposed model was validated by using it to represent a transmission line during short-and open-circuit tests. The results obtained with the proposed model were compared with results obtained with a classical model based on modal decomposition. These comparisons show that proposed model was correctly developed. © 2013 Taylor and Francis Group, LLC.

Characterization of 23S rRNA domain V mutations in gastric biopsy patients from the eastern Amazon

Resistance of Helicobacter pylori to clarithromycin is characterised by simple point mutations in the 23S ribosomal RNA (rRNA) gene and is responsible for the majority of cases of failure to eradicate this bacterium. In this paper, we characterised the variability of the 23S rRNA gene in biopsies of patients with gastric pathologies in the eastern Amazon (Northern Region of Brazil) using PCR and sequencing. A total of 49 sequences of H. pylori strains were analysed and of those, 75.6% presented nucleotide substitutions: A2142G (3.3%), T2182C (12.9%), G2224A (6.45%), T2215C (61.3%), A2192G (3.3%), G2204C (6.4%) and T2221C (6.4%). Of the mutations identified, four are known mutations related to cases of resistance and 16.1% are not yet described, revealing a high prevalence of mutations in the H. pylori 23S rRNA gene among the strains circulating in the in the eastern Amazon. The high prevalence in individuals with gastric pathologies in the Northern Region of Brazil demonstrates the need for characterising the profile of these strains to provide correct therapy for patients, considering that mutations in this gene are normally associated with resistance to the primary medication used in controlling H. pylori infection.

Domain wall description of superconductivity

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Exercise domain profile through pulmonary gas exchange response during kendo practice by men

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Phase transitions and interface phenomena in the cryogenic temperature domain of a niobate nanostructured ceramic

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Bibliometrics Contribution to the Metatheoretical and Domain Analysis Studies

Along with the growth of scientific production, the bibliometric studies have become essential, providing relevant information about any domain while identifying, highlighting and viewing the scientific knowledge constructed within a theme, subject or knowledge area. This article approaches the bibliometric studies under the light of metatheory and domain analysis within the knowledge organization in information science. domain analysis and metatheory are considered significant contribution to bibliometric studies when emphasizing the need for epistemological, sociological and historical analyzes, as well as other qualitative approaches, encouraging researchers to reflect on the whole composition of the research object, by means of different methodological, theoretical and epistemological approaches. This study aims at contributing to the discourse of the theoretical aspects of bibliometric studies by presenting considerations concerning Hjorland's domain analysis, as well as Ritzer's metatheoretical approach, featuring discussions on major theories of a study field, since these approaches together provide a more complete qualitative view upon an area, here, represented by the bibliometric studies.

Reliability of Cardiorespiratory Parameters During Cycling Exercise Performed at the Severe Domain in Active Individuals

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Static domain wall in braneworld gravity

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

A NOTE ON RESOLVENT CONVERGENCE ON A THIN DOMAIN

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Studies of Author Cocitation Analysis: A Bibliometric Approach for Domain Analysis

Author Cocitation Analysis (ACA) can be defined as the analysis of a group of actors, writers or researchers structurally organized in a (social and cognitive) network of a particular scientific community. The greater the number of researchers selected, the greater the amplitude and the domain boundary under consideration; the more restricted the number of researchers chosen as representative and appropriate, the less extensive the domain. From the perspective of the first axis of Tennis (2003), the selection of authors involves setting parameters on the extent of the domain, i.e., its total scope and amplitude. Thus, from the point of view of Tennis’s (2003) approach to Domain Analysis, the selection of authors for Author Cocitation Analysis is associated with the designations and boundaries of the domain, as well as to their goals (Tennis, 2003). Still, the selection of authors through the most cited authors in the literature, reflects the core elements of a domain and constitute the most specific foundation of a domain, aligning to the Degrees of Specialization characterized by Tennis (2003). It is concluded that the Author Cocitation Analysis (ACA) is a relevant procedure to the analysis of the underlying structure of a scientific knowledge domain, which meets the theories and concepts of Domain Analysis researchers, in that it allows characterizing the science, identifying, analyzing and assessing the conditions under which scientific knowledge is constructed and socialized.