940 resultados para Quantitative Real-time Pcr
Resumo:
Evans D A, Roberts O R, Vearey-Roberts A R, Langstaff D P, Twitchen D J and Schwitters M 2007 Direct observation of Schottky to ohmic transition in Al-diamond contacts using realtime photoelectron spectroscopy Appl. Phys. Lett. 91 132114 doi:10.1063/1.2790779
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Real-time adaptive music is now well-established as a popular medium, largely through its use in video game soundtracks. Commercial packages, such as fmod, make freely available the underlying technical methods for use in educational contexts, making adaptive music technologies accessible to students. Writing adaptive music, however, presents a significant learning challenge, not least because it requires a different mode of thought, and tutor and learner may have few mutual points of connection in discovering and understanding the musical drivers, relationships and structures in these works. This article discusses the creation of ‘BitBox!’, a gestural music interface designed to deconstruct and explain the component elements of adaptive composition through interactive play. The interface was displayed at the Dare Protoplay games exposition in Dundee in August 2014. The initial proof-of- concept study proved successful, suggesting possible refinements in design and a broader range of applications.
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In this paper, we propose a new class of Concurrency Control Algorithms that is especially suited for real-time database applications. Our approach relies on the use of (potentially) redundant computations to ensure that serializable schedules are found and executed as early as possible, thus, increasing the chances of a timely commitment of transactions with strict timing constraints. Due to its nature, we term our concurrency control algorithms Speculative. The aforementioned description encompasses many algorithms that we call collectively Speculative Concurrency Control (SCC) algorithms. SCC algorithms combine the advantages of both Pessimistic and Optimistic Concurrency Control (PCC and OCC) algorithms, while avoiding their disadvantages. On the one hand, SCC resembles PCC in that conflicts are detected as early as possible, thus making alternative schedules available in a timely fashion in case they are needed. On the other hand, SCC resembles OCC in that it allows conflicting transactions to proceed concurrently, thus avoiding unnecessary delays that may jeopardize their timely commitment.
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Various concurrency control algorithms differ in the time when conflicts are detected, and in the way they are resolved. In that respect, the Pessimistic and Optimistic Concurrency Control (PCC and OCC) alternatives represent two extremes. PCC locking protocols detect conflicts as soon as they occur and resolve them using blocking. OCC protocols detect conflicts at transaction commit time and resolve them using rollbacks (restarts). For real-time databases, blockages and rollbacks are hazards that increase the likelihood of transactions missing their deadlines. We propose a Speculative Concurrency Control (SCC) technique that minimizes the impact of blockages and rollbacks. SCC relies on the use of added system resources to speculate on potential serialization orders and to ensure that if such serialization orders materialize, the hazards of blockages and roll-backs are minimized. We present a number of SCC-based algorithms that differ in the level of speculation they introduce, and the amount of system resources (mainly memory) they require. We show the performance gains (in terms of number of satisfied timing constraints) to be expected when a representative SCC algorithm (SCC-2S) is adopted.
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Predictability — the ability to foretell that an implementation will not violate a set of specified reliability and timeliness requirements - is a crucial, highly desirable property of responsive embedded systems. This paper overviews a development methodology for responsive systems, which enhances predictability by eliminating potential hazards resulting from physically-unsound specifications. The backbone of our methodology is a formalism that restricts expressiveness in a way that allows the specification of only reactive, spontaneous, and causal computation. Unrealistic systems — possessing properties such as clairvoyance, caprice, infinite capacity, or perfect timing — cannot even be specified. We argue that this "ounce of prevention" at the specification level is likely to spare a lot of time and energy in the development cycle of responsive systems - not to mention the elimination of potential hazards that would have gone, otherwise, unnoticed.
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This paper describes an algorithm for scheduling packets in real-time multimedia data streams. Common to these classes of data streams are service constraints in terms of bandwidth and delay. However, it is typical for real-time multimedia streams to tolerate bounded delay variations and, in some cases, finite losses of packets. We have therefore developed a scheduling algorithm that assumes streams have window-constraints on groups of consecutive packet deadlines. A window-constraint defines the number of packet deadlines that can be missed in a window of deadlines for consecutive packets in a stream. Our algorithm, called Dynamic Window-Constrained Scheduling (DWCS), attempts to guarantee no more than x out of a window of y deadlines are missed for consecutive packets in real-time and multimedia streams. Using DWCS, the delay of service to real-time streams is bounded even when the scheduler is overloaded. Moreover, DWCS is capable of ensuring independent delay bounds on streams, while at the same time guaranteeing minimum bandwidth utilizations over tunable and finite windows of time. We show the conditions under which the total demand for link bandwidth by a set of real-time (i.e., window-constrained) streams can exceed 100% and still ensure all window-constraints are met. In fact, we show how it is possible to guarantee worst-case per-stream bandwidth and delay constraints while utilizing all available link capacity. Finally, we show how best-effort packets can be serviced with fast response time, in the presence of window-constrained traffic.
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The proliferation of mobile computers and wireless networks requires the design of future distributed real-time applications to recognize and deal with the significant asymmetry between downstream and upstream communication capacities, and the significant disparity between server and client storage capacities. Recent research work proposed the use of Broadcast Disks as a scalable mechanism to deal with this problem. In this paper, we propose a new broadcast disks protocol, based on our Adaptive Information Dispersal Algorithm (AIDA). Our protocol is different from previous broadcast disks protocols in that it improves communication timeliness, fault-tolerance, and security, while allowing for a finer control of multiplexing of prioritized data (broadcast frequencies). We start with a general introduction of broadcast disks. Next, we propose broadcast disk organizations that are suitable for real-time applications. Next, we present AIDA and show its fault-tolerance and security properties. We conclude the paper with the description and analysis of AIDA-based broadcast disks organizations that achieve both timeliness and fault-tolerance, while preserving downstream communication capacity.
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We propose and evaluate an admission control paradigm for RTDBS, in which a transaction is submitted to the system as a pair of processes: a primary task, and a recovery block. The execution requirements of the primary task are not known a priori, whereas those of the recovery block are known a priori. Upon the submission of a transaction, an Admission Control Mechanism is employed to decide whether to admit or reject that transaction. Once admitted, a transaction is guaranteed to finish executing before its deadline. A transaction is considered to have finished executing if exactly one of two things occur: Either its primary task is completed (successful commitment), or its recovery block is completed (safe termination). Committed transactions bring a profit to the system, whereas a terminated transaction brings no profit. The goal of the admission control and scheduling protocols (e.g., concurrency control, I/O scheduling, memory management) employed in the system is to maximize system profit. We describe a number of admission control strategies and contrast (through simulations) their relative performance.
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This report summarizes the technical presentations and discussions that took place during RTDB'96: the First International Workshop on Real-Time Databases, which was held on March 7 and 8, 1996 in Newport Beach, California. The main goals of this project were to (1) review recent advances in real-time database systems research, (2) to promote interaction among real-time database researchers and practitioners, and (3) to evaluate the maturity and directions of real-time database technology.
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Load balancing is often used to ensure that nodes in a distributed systems are equally loaded. In this paper, we show that for real-time systems, load balancing is not desirable. In particular, we propose a new load-profiling strategy that allows the nodes of a distributed system to be unequally loaded. Using load profiling, the system attempts to distribute the load amongst its nodes so as to maximize the chances of finding a node that would satisfy the computational needs of incoming real-time tasks. To that end, we describe and evaluate a distributed load-profiling protocol for dynamically scheduling time-constrained tasks in a loosely-coupled distributed environment. When a task is submitted to a node, the scheduling software tries to schedule the task locally so as to meet its deadline. If that is not feasible, it tries to locate another node where this could be done with a high probability of success, while attempting to maintain an overall load profile for the system. Nodes in the system inform each other about their state using a combination of multicasting and gossiping. The performance of the proposed protocol is evaluated via simulation, and is contrasted to other dynamic scheduling protocols for real-time distributed systems. Based on our findings, we argue that keeping a diverse availability profile and using passive bidding (through gossiping) are both advantageous to distributed scheduling for real-time systems.
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The design of programs for broadcast disks which incorporate real-time and fault-tolerance requirements is considered. A generalized model for real-time fault-tolerant broadcast disks is defined. It is shown that designing programs for broadcast disks specified in this model is closely related to the scheduling of pinwheel task systems. Some new results in pinwheel scheduling theory are derived, which facilitate the efficient generation of real-time fault-tolerant broadcast disk programs.
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There is an increased interest in using broadcast disks to support mobile access to real-time databases. However, previous work has only considered the design of real-time immutable broadcast disks, the contents of which do not change over time. This paper considers the design of programs for real-time mutable broadcast disks - broadcast disks whose contents are occasionally updated. Recent scheduling-theoretic results relating to pinwheel scheduling and pfair scheduling are used to design algorithms for the efficient generation of real-time mutable broadcast disk programs.
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The advent of virtualization and cloud computing technologies necessitates the development of effective mechanisms for the estimation and reservation of resources needed by content providers to deliver large numbers of video-on-demand (VOD) streams through the cloud. Unfortunately, capacity planning for the QoS-constrained delivery of a large number of VOD streams is inherently difficult as VBR encoding schemes exhibit significant bandwidth variability. In this paper, we present a novel resource management scheme to make such allocation decisions using a mixture of per-stream reservations and an aggregate reservation, shared across all streams to accommodate peak demands. The shared reservation provides capacity slack that enables statistical multiplexing of peak rates, while assuring analytically bounded frame-drop probabilities, which can be adjusted by trading off buffer space (and consequently delay) and bandwidth. Our two-tiered bandwidth allocation scheme enables the delivery of any set of streams with less bandwidth (or equivalently with higher link utilization) than state-of-the-art deterministic smoothing approaches. The algorithm underlying our proposed frame-work uses three per-stream parameters and is linear in the number of servers, making it particularly well suited for use in an on-line setting. We present results from extensive trace-driven simulations, which confirm the efficiency of our scheme especially for small buffer sizes and delay bounds, and which underscore the significant realizable bandwidth savings, typically yielding losses that are an order of magnitude or more below our analytically derived bounds.
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SomeCast is a novel paradigm for the reliable multicast of real-time data to a large set of receivers over the Internet. SomeCast is receiver-initiated and thus scalable in the number of receivers, the diverse characteristics of paths between senders and receivers (e.g. maximum bandwidth and round-trip-time), and the dynamic conditions of such paths (e.g. congestion-induced delays and losses). SomeCast enables receivers to dynamically adjust the rate at which they receive multicast information to enable the satisfaction of real-time QoS constraints (e.g. rate, deadlines, or jitter). This is done by enabling a receiver to join SOME number of concurrent multiCAST sessions, whereby each session delivers a portion of an encoding of the real-time data. By adjusting the number of such sessions dynamically, client-specific QoS constraints can be met independently. The SomeCast paradigm can be thought of as a generalization of the AnyCast (e.g. Dynamic Server Selection) and ManyCast (e.g. Digital Fountain) paradigms, which have been proposed in the literature to address issues of scalability of UniCast and MultiCast environments, respectively. In this paper we overview the SomeCast paradigm, describe an instance of a SomeCast protocol, and present simulation results that quantify the significant advantages gained from adopting such a protocol for the reliable multicast of data to a diverse set of receivers subject to real-time QoS constraints.
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A human-computer interface (HCI) system designed for use by people with severe disabilities is presented. People that are severely paralyzed or afflicted with diseases such as ALS (Lou Gehrig's disease) or multiple sclerosis are unable to move or control any parts of their bodies except for their eyes. The system presented here detects the user's eye blinks and analyzes the pattern and duration of the blinks, using them to provide input to the computer in the form of a mouse click. After the automatic initialization of the system occurs from the processing of the user's involuntary eye blinks in the first few seconds of use, the eye is tracked in real time using correlation with an online template. If the user's depth changes significantly or rapid head movement occurs, the system is automatically reinitialized. There are no lighting requirements nor offline templates needed for the proper functioning of the system. The system works with inexpensive USB cameras and runs at a frame rate of 30 frames per second. Extensive experiments were conducted to determine both the system's accuracy in classifying voluntary and involuntary blinks, as well as the system's fitness in varying environment conditions, such as alternative camera placements and different lighting conditions. These experiments on eight test subjects yielded an overall detection accuracy of 95.3%.