北京东灵山地区森林植物养分利用效率研究

本文选择东灵山地区具有代表性的11种植物，研究了植物叶片养分元素动态特征以及各个层次物种、生活型和生境等对植物叶片养分元素再吸收的影响。主要结论如下： 1、不同物种间、以及相同物种在不同森林类型内的叶片C素含量变化程度较小;11个物种之间叶片N素养分浓度差很大，但各种植物叶片的N素含量的季节变化比较一致，大多数情况下表现为单峰型或双峰变化曲线;各种森林类型中11种植物叶片P的最高含量都出现在生长初期，以后开始下降。 2、在物种水平、养分再吸收是一种重要的养分保存机制，N素和P素再吸收率大约为50%，对这两种养分元素而言，相同生境下生长的不同植物种的养分再吸收率不同，存在显著差异，而同一植物种不同生境下养分再吸收率之间也存在差异，而S素的差异则不显著。 3、同一生活型在不同的森林类型中养分再吸收率差异不明显，同一森林类型中不同生活型的养分再吸收率差异也不明显;高养分再吸收率不是低养分环境所选择的优势性状，低养分环境中的物种主要通过延长叶片寿命和加强低养分浓度状况下的物质合成来适应低养分环境。 4、除了土壤pH值外，土壤容重、土壤有机质、土壤全氮、土壤阳离子交换量在三种生境下都表现出一定的差异性。华北落叶松林生境下的土壤养分含量较丰富，其次是落叶阔叶混交林，辽东栎林相对前二种生境其土壤养分相对低一些，但在三种生境之间养分元素再吸收率差异不显著，植物养分再吸收没有或微弱地受控于土壤养分获得的能力。

神农架植被及其生物多样性与垂直分布格局-兼论中国中、北亚热带植被带的划分

本文主要通过样线法和样方法相结合，进行了大量的群落学调查和分析，分别从植物区系、物种多样性的垂直分布格局和森林群落类型三个方面分析了神农架植被的基本特征及其物种多样性，结果表明： 1．神农架地区具有很高的物种丰富度，有高等植物3，479种，隶属于1，010属，202科。 其中，蕨类植物305种，80属，32科;种子植物3，174种，930属，170科，其中裸子植物32种，19属，6科，被子植物3，142种，911属，164科;单子叶植物501种，175属，21科，双子叶植物2，641种，736属，143科。植物区系属的分布区类型中北温带分布型最多，其次为东亚分布、泛热带分布、东亚北美间断分布、旧世界温带分布以及热带亚洲分布。中国特有成分占5.65%，较全国的8.12%低。温热比(温带分布型(8-11)属数与热带分布型(2-7)属数的比值）为1.200，比全国(0.385)高。 调查样方中共出现高等植物784种，隶属于454属，144科，其中蕨类植物41种，32属，16科;种子植物743种，422属，128科，其中裸子植物20种，14属，5科，被子植物723种，408属，123科;单子叶植物86种，58属，11科，双子叶植物637种，350属，112科。属的分布区类型中北温带分布型最多，其次为东亚分布、泛热带分布、东亚北美间断分布、旧世界温带分布以及热带亚洲分布。温热比为1.52，草本层>乔木层>灌木层分别为2.18、1.76和1.14。 2．神农架植被类型多样，具有常绿阔叶林、常绿落叶阔叶混交林、落叶阔叶林、针阔混交林、亚高山针叶林、硬叶常绿阔叶林和亚高山灌丛草甸等自然植被类型。本文，依据乔木物种的重要值将神农架地区的森林植被划分出了69个类型。用Twinspan将调查的森林群落划分为32组，能基本上反映群落间相似的关系。 3．神农架地区具有完整的植被垂直带谱：海拔900 (1300) m以下为常绿阔叶林带;海拔900 (1300) m～1500 (1800)ⅡI为常绿落叶阔叶混交林带;海拔1500 (1800) m-2000 (2200)m为落叶阔叶林带;海拔2000 (2200) m～2400 (2600)m为针阔混交林带：海拔2400 (2600)m以上为亚高山针叶林带。神农架地区植被的垂直带的分化从总体上比较显著，但由于小生境的异质性和人为干扰，垂直带谱又具有一定的模糊性和次生性。南北坡具有一定的差异，但不十分明显，也说明神农架植被的过渡性。 4．神农架物种多样性的垂直分布格局。神农架的物种多样性与海拔的关系，类似于“中间膨胀”规律(mid-altitude bulge)，在中低海拔处生物多样性最高。通过二次多项式回归拟合，得到如下拟合曲线： 1)海拔与总体物种数：y= _14.445x2+ 34.74lx+42.07，Xd=1.203km; 2)海拔与乔木层物种数：y=-6.9707x2+ 21.334x+0.2004，Xdrl.530km; 3)海拔与灌木层物种数：y=-6.1599x2+ 9.9747x+30.991，Xd=0.8 lOkm: 4)海拔与草本层物种数：y= _3.9907x2+ 10.455x+15.35，Xd-1.308km; 5）海拔与乔木层Shannon-Wiener指数：y=_0.3337x2+ 0.9877x+0.2537，Xd' 1.480km; 6)海拔与灌木层Shannon-Wiener指数：y=-0.1938xz+ 0.422lx+1.2103，Xd=1.089km： 7)海拔与草本层Shannon-Wiener指数：y=_0.1072x2+ 0.294lx+0.9954，Xd=1.372km; x为海拔( km)，y为各物种多样性指标，Xd为物种多样性的最大时的海拔。 从这些拟合曲线中可以看出：总体物种多样性在海拔1200m左右的常绿落叶阔叶混交林带最高：乔木层物种多样性在海拔1500m左右的常绿落叶阔叶混交林带与落叶阔叶林的过渡带最高;灌木层物种多样性在海拔800-llOOm左右的常绿阔叶林与常绿落叶阔叶混交林带的过渡带最高;草本层物种多样性在海拔1300-1400m左右的常绿落叶阔叶混交林带最高。 但物种多样性随海拔变化有许多的起伏和波动。这些波动有些反映了群落的垂直带谱随海拔梯度变化的特点，在垂直带谱的过渡区物种多样性往往较高;有些波动反映了一些特殊的生境，有些反映了人为活动的影响，造成了神农架植被的次生性。因此，影响神农架物种多样性垂直分布的因素有：植被本身的性质和特点、过渡带的特点、生境的异质性和人为活动。 5．神农架植被水平地带性的过渡性。海拔1300m以下的植物属的分布区类型的温热比南坡总是比北坡小，而且相差十分显著，反映了神农架作为植被分界线的价值。神农架南坡的基带植被是常绿阔叶林，因此南坡属于中亚热带。北坡的基带植被，虽然也有常绿树种的零星分布，甚至有小块的常绿阔叶林，完全由于小生境所至，分布的主要类型是常绿落叶阔叶混交林，应属于北亚热带。因此，神农架是中、北亚热带重要的过渡地带。神农架地区中北亚热带的具体分界线宜按照分长江干流和汉水的水岭来划界，即猴子石、大窝坑、神农架、神农顶、老君山一线，南坡属于中亚热带，北坡属于北亚热带。 总之，神农架处于我国中、北亚热带的过渡带，具有过渡带的性质，具有很高的物种多样性，拥有完整的植被垂直带谱，具有多种多样的植物群落及其组成的生态系统。而且，具有我国许多特有植物和珍稀濒危保护植物和许多资源植物。因此，神农架植被在我国植被体系中具有重要的地位，是我国生物多样性最丰富的地区之一，是生物多样性保护的关键地区，也应是生物多样性研究的热点地区。 另外，调查分析了黄山和万朝山植被及其物种多样性与垂直分布格局，结果表明： 6．黄山样方中共出现高等植物259种，隶属于263属，110科，其中蕨类植物14种，II属，8科，种子植物345种，152属，105科，其中裸子植物9种，8属，6科，被子植物336种，144属，99科，其中单子叶植物37种，27属，6科，双子叶植物299种，117属，90科。属的分布区类型中北温带分布最多，其次为东亚分布和泛热带分布，再次为东亚北美间断分布、热带亚洲分布以及旧世界温带分布，与神农架和万朝山也较相似，但热带分布的属更多一些。温热比为1.1875，灌木层>草本层>乔木层，分别为1.3818、1.2609和1.2143。 黄山的森林植被类型有针叶林、常绿阔叶林、常绿落叶阔叶混交林、针阔混交林、落叶阔叶林和竹林。Twinspan将调查的森林群落划分为22组，反映群落间相似的关系，比较清楚和适用。依据乔木物种的重要值将森林植被划分出了34个类型。黄山物种多样性的与海拔的关系不十分明显。黄山植被的垂直带谱不是十分明显，将其垂直带谱划分为：海拔1300m(1500m)以下为常绿阔叶林带;海拔1300m(1500m)-1500m(1600m)常绿落叶阔叶混交林 带;1500m(1600m)以上为落叶阔叶林、黄山松林、山地灌木草丛带。垂直带谱在不同坡向上有差别，东、南、西坡的相似性较大，而北坡与其差别较大。 7．万朝山样方中共出现高等植物490种，隶属于339属，124科，其中蕨类植物21种，18属，11科，种子植物469种，321属，113科，其中裸子植物9种，7属，4科，被子植物460种，314属，109科，其中单子叶植物47种，37属，11科，双子叶植物413种，277属，98科。植物属的分布区类型中，北温带分布所占最多，其次为泛热带分布、东亚分布、东亚北美间断分布、旧世界温带分布以及热带亚洲分布，。温热比为1.3366，草本层>乔木层>灌木层，分别为1.5429、1.4063和1.0645。 万朝山的植被类型包括针叶林、落叶阔叶林、针阔混交林和常绿落时阔叶混交林，但没有典型的常绿阔叶林。依据乔木物种的重要值将森林植被划分出了20个类型。万朝山物种多样性与海拔的关系则不十分明显。万朝山的人为干扰比较强，植被的次生性很大，南、北坡物种多样性随海拔升高的起伏较大。

Fire, climate, and vegetation: the sedimentary record of fire in montane meadows, Sierra Nevada, California, USA [abstract]

EXTRACT (SEE PDF FOR FULL ABSTRACT): Though knowledge of fire occurrence and weather pattern relationships has been used for many years by land managers in, for instance, prescribed fire planning, understanding of the relationship between Holocene climates and fire is just beginning to be investigated. We are investigating this relationship in a major mountain range in California, examining charcoal and pollen content in sediments of montane meadows to compare paleo-fire and paleo-vegetation (thus, climate) sequences for the Holocene.

The Little Ice Age: glacier variations and climate since AD 1250 [abstract]

EXTRACT (SEE PDF FOR FULL ABSTRACT): During the past hundred years, mountain glaciers throughout the world have retreated significantly from moraines built during the previous several centuries. In the 1930s, Francois Matthes of the U.S. Geological Survey concluded that the moraines represent the greatest advances of glaciers since the end of the last glacial age, some 10,000 years earlier, and informally referred to this late Holocene interval of expanded ice cover as the Little Ice Age.

新疆天山中段北坡地区现代孢粉学研究

新疆中部地区位于西北内陆干旱一半干旱区，是欧亚大陆的地理中心，是中纬度内陆干旱区代表性区域，也是全球变化敏感区域之一，基本特点是气候干旱、水资源匮乏、植被稀少、荒漠广布，生态系统脆弱。在研究区域内，天山山脉海拔高度差异5 000 m左右，随海拔梯度下降依次出现高山冰雪带、高山亚高山草甸带、森林带、草原带、荒漠带、沙漠带等明显完整的植被垂直带，是开展现代孢粉学和植被变化研究的理想地点。本报告在立足于该区前人工作的基础上，从表土花粉、空气花粉和现代植被之间的关系入手开展现代孢粉学研究，探讨了花粉与植被的关系。 在研究区内，选择新疆阜康市和吉木萨尔县为研究地点，设计了一条从天山博格达峰附近雪线开始直至古尔班通古特沙漠长约100 km、宽约20 km的样带。沿样带，从高山座垫植被和草甸带开始，经过中海拔地带的云杉林，置到低海拔的荒漠，在山地海拔每下降20～100 m采集一个表土花粉样品，平原每间隔10～20 km采集一个表土花粉样品，同时做现代植被调查，共采集80个表土花粉样品和调查了86个现代植被样方;在天山乌鲁木齐河河源区的大西沟，从一号冰川前缘开始，沿山谷向下直到谷口，按海拔100 m间隔采集了14个表土样品，对表土花粉与植被之间的关系进行了探讨。为了弄清空气花粉传播、散布与气象因子和植被的关系，沿样带从高山到沙漠分别在天池气象站、中国科学院阜康荒漠生态系统定位研究站和北沙窝草炭试验地设置了三个风标式空气花粉收集器，从2001年7月至2002年7月连续收集空气花粉雨，收集了跨2个年度1周年的1 14个空气花粉样品，依据分析结果研究了空气花粉的传播和散布规律以及与表土花粉和植被之间的关系，这在新疆地区乃至全国尚属首次。 根据表土花粉分析结果和现代植被样方资料的分析以及天山中段主要植物种类沿海拔高度的分布特征，天山中段北坡垂直植被带谱为：高山座垫植被(>3 400 m)、高山亚高山草甸(3 400-2 700 m)、山地云杉林(2 700-1 720 m)、森林草原过渡带(1 720-1 300 m)、蒿类荒漠(1 300-700 m)和典型荒漠(<700 m)。表土花粉谱基本上反映了相同海拔高度的现代植被带，特别是在云杉林带，表土中雪岭云杉(Picea schrenkiana)花粉占优势;在蒿类荒漠植被带表土中蒿属（Artemisia）植物花粉非常丰富;在典型荒漠植被带的表土中藜科(Chenopodiaceae)植物花粉占绝对优势，因此可依据这些建群种植物花粉含量峰值的位置划分植物群落。 空气花粉样品分析结果表明：空气中花粉主要是现生植物当年生长产出的花粉，盛花期的花粉数量能较好地反映当地的植被状况。空气中的乔木植物花粉主要有云杉(Picea)，其次是柳属（Salix）;灌木草本植物花粉主要是藜科和蒿属，其次是菊科(Compositae)、禾本科(Gramineae)、柽柳科(Tamaricaceae)和麻黄属(Ephedra);空气中的花粉数量与植物的花期相对应，云杉等乔木植物盛花期在夏季5月末至7月初，灌木草本植物盛花期在夏秋季节。气温、降水、风速和风向等气象条件对空气中花粉数量有很大影响，其中风速和风向影响最大。从空气花粉和表土花粉的分析结果与现生植被对比，新疆中部地区的山谷上升气流是导致花粉“爬坡”现象的主要动力，在一定条件下，上升气流搬运花粉的数量和能力是十分可观的。 空气中的花粉是现生植物当年生长产出的花粉，表土中的花粉是多年花粉落地沉积的积累，植物盖度大，该植物的花粉在空气中和表土中数量相对就大。在新疆中部地区，空气花粉能反映当地和附近周边方圆约50 km的植被状况，其花粉组合特征与表土花粉和当地现生植被分布的对比研究，可以较好地寻找花粉与植被的关系。本研究报告的研究成果不仅为新疆地区的孢粉学研究增添新的内容，而且将有助于合理解释新疆地层中、尤其是第四纪以来的地层孢粉，较客观地恢复过去的植被景观，同时可为本地区的大气环境检测提供有重要价值的参考资料。

暖温带典型森林生态系统未来碳平衡的LPJ-GUESS模型模拟与分析

森林作为陆地生态系统的主体，在全球陆地碳循环中起着决定性作用。实测和模型研究均表明北半球的森林是重要的大气CO2汇，在缓解全球碳收支失衡中发挥着关键作用。过去几十年北半球所经历的显著气候变化，已经很大地改变了陆地生态系统的碳平衡状况。随着未来100年气候变化继续增大，对未来气候变化下森林生态系统碳平衡的预测研究就尤为重要。 北京山区森林属于典型的暖温带森林生态系统，前人对本区森林的植被特征、生态系统结构和功能、养分循环以及长期动态变化等都进行了深入的研究。然而长期的人类活动已使本区原生的地带性植被破坏殆尽。因此，对该区域森林生态系统碳平衡的模拟研究可以帮助我们认识其生态系统碳平衡变化特点及未来气候变化对其潜在的影响。 本研究采用LPJ-GUESS植被动态机理模型，利用IPCC于2000年发布的《排放情景特别报告》(SRES)的A2和B2两种情景下不同气候模式对华北地区未来100年温度和降水预测的平均值以及相应大气CO2浓度变化情景进行驱动，模拟北京山区未来100年暖温带森林生态系统的净初级生产力和碳平衡，尽可能真实地反映未来百年的变化趋势。通过比较当前和未来气候情景下北京山区以辽东栎（Quercus liaotungensis）为优势种的落叶阔叶林、以白桦（Betula platyphylla）为主的落叶阔叶林和油松（Pinus tabulaeformis）为主的针阔混交林三种典型暖温带森林生态系统的碳平衡差异，了解未来北京山区这三种暖温带森林生态系统的碳源汇功能，认识气候变化和大气CO2浓度升高对净初级生产力（Net primary productivity, NPP）、净生态系统碳交换（Net ecosystem exchange, NEE）、土壤异养呼吸（Heterotrophic respiration, Rh）和碳储量（Carbon biomass, C biomass）的影响，以及不同生态系统碳平衡对气候变化响应的异质性。 结果表明，未来100年两种气候情景下三种森林生态系统的NPP和Rh均增加，并且A2情景下增加的程度更大。由于三种生态系统树种组成的不同，未来气候情景下各自NPP和Rh增加的比例不同，导致三者NEE的变化也相异：100年后辽东栎林由碳汇转变为弱碳源，白桦林仍保持为碳汇但功能减弱，油松林成为一个更大的碳汇。三种森林生态系统的碳生物量在未来气候情景下均增大，21世纪末与20世纪末相比：辽东栎林在A2情景下碳生物量增加的比例为27.6%，大于B2情景下的19.3%；白桦林和油松林在B2情景下碳生物量增加的比例分别为34.2%和52.2%，大于A2情景下的30.8%和28.4%。各森林类型碳平衡状况不同，原因除气候因素外，主要是由于树种组成的差异所导致。SRES A2和B2两种气候情景相比，相对较低的排放情景（B2）下，生态系统有更高的碳储量。

Results of the Austrian-Ceylonese hydrobiological mission 1970 of the 1st Zoological Institute of the University of Vienna (Austria) and the Department of Zoology of the Vidyalankara University of Ceylon, Kelaniya. Pt. 1. Preliminary report: introduction and description of the stations

The Austrian-Ceylonese hydrobiological mission of 1970 investigated and made collections from 36 flowing water systems (brooks, torrents, rivers); of these, 34 water systems were in the mountains regions of south-west and south-east of Sri Lanka. In the crystalline mountain region, the water systems are extremely poor in electrolytes, very soft and slightly acid; these torrential streams have strong falls, high flow velocities and boulder bottoms. The water temperatures increase from the sources and brooks at 2,000 m altitude to the mouths from 15°C to 28°C. The density of animal population (macro and meso-fauna) increases from the river bank regions (and pools) towards the sections with strong current and reaches on the rocks in the cascades a density of 500 to appr. 750 individuals/1/16m².

Results of the Austrian-Ceylonese hydrobiological mission 1970 of the 1st Zoological Institute of the University of Vienna (Austria) and the Department of Zoology of the Vidyalankara University of Ceylon, Kelaniya. Pt. 5. Decapoda Caridea

The first comprehensive study on the freshwater Caridea of Sri Lanka was by Arudpragrasm and Costa in 1961. Although it was the intention of these authors to continue this study no opportunity was available to them. The present faunal survey however has afforded the present writer to make a detailed study of the distribution of these shrimps especially in the mountain streams of the south-west of Sri Lanka. Two species and two sub-species of Caridina, one species of Atya and four species of Macrobrachium were collected by the hydrobiological mission from the hill streams of Sri Lanka.

Results of the Austrian-Ceylonese hydrobiological mission 1970 of the 1st Zoological Institute of the University of Vienna (Austria) and the Department of Zoology of the University of Ceylon, Vidyalankara Campus, Kelaniya (Sri Lanka). [Pt. 15.] Collection of fishes (Osteichthyes)

A total of 378 specimens from 25 collecting localities belonging to 31 different species of fish collected mainly from the rivers of the hilly and mountain regions of the south-western and southern Ceylon have been identified and recorded. Ecological data and water analyses of these collecting localities are given.

Sanitation, fish handling and artisanal fish processing within fishing communities: socio-cultural influences

postive transformation of the fisheries sector in Uganda has of recent been scatted by failure to mountain fish quality and safety,akey prerequiste for retaining and gaining fish markets. The social cultural study established the extent to which social cutural practices had affected the levels and the use of sanitation facilities,fish handling facilities and artisanal fish processing techniques and the factors that influenced these practices in the fishing communities of lake victoria. This information is of significance for the purpose of advocacy and mobilization of fishers in order to improve sanitation, fish handling and artisanal fishing processing situation in the fishing communities.

Hydrobiological status of a lesser Himalayan river

It is generally observed that a variety of physical and chemical variables have considerable impact on the biological life in a mountain river which include plankton, benthic algae, benthic invertebrate and finally the fishery resources. They are often subjected to extreme hydrological disturbances particularly during rainy seasons when increased volume of water create 'Wash Off' situation for existing fauna and flora. A case study of a lesser Himalayan river - the Gaula, which drains the south-central part of Kumaon region in Uttaranchal is presented here. Since this river does not get snow-melt water, it mainly depends on steady flow of the groundwater round the year. The climate of the area is characterized with long winters, short summer and good amount of rain during monsoon months. The infiltrated groundwater which seeps into the surface soil layers during monsoon seasons is the chief source of discharge of water during winter and summer season

Comparative molecular cytogenetic studies in the order Carnivora: mapping chromosomal rearrangements onto the phylogenetic tree

We have made a set of chromosome-specific painting probes for the American mink by degenerate oligonucleotide primed-PCR (DOP-PCR) amplification of flow-sorted chromosomes. The painting probes were used to delimit homologous chromosomal segments among human, red fox, dog, cat and eight species of the family Mustelidae, including the European mink, steppe and forest polecats, least weasel, mountain weasel, Japanese sable, striped polecat, and badger. Based on the results of chromosome painting and G-banding, comparative maps between these species have been established. The integrated map demonstrates a high level of karyotype conservation among mustelid species. Comparative analysis of the conserved chromosomal segments among mustelids and outgroup species revealed 18 putative ancestral autosomal segments that probably represent the ancestral chromosomes, or chromosome arms, in the karyotype of the most recent ancestor of the family Mustelidae. The proposed 2n = 38 ancestral Mustelidae karyotype appears to have been retained in some modern mustelids, e.g., Martes, Lutra, ktonyx, and Vormela. The derivation of the mustelid karyotypes from the putative ancestral state resulted from centric fusions, fissions, the addition of heterochromatic arms, and occasional pericentric inversions. Our results confirm many of the evolutionary conclusions suggested by other data and strengthen the topology of the carnivore phylogenetic tree through the inclusion of genome-wide chromosome rearrangements. Copyright (C) 2002 S. KargerAG, Basel.

Molecular systematics of pikas (genus Ochotona) inferred from mitochondrial DNA sequences

The phylogenetic relationships among worldwide species of genus Ochotona were investigated by sequencing mitochondrial cytochrome b and ND4 genes. Parsimony and neighbor-joining analyses of the sequence data yielded congruent results that strongly indicated three major clusters: the shrub-steppe group, the northern group, and the mountain group. The subgeneric classification of Ochotona species needs to be revised because each of the two subgenera in the present classification contains species from the mountain group. To solve this taxonomic problem so that each taxon is monophyletic, i.e., represents a natural clade, Ochotona could be divided into three subgenera, one for the shrub-steppe species, a second for the northern species, and a third for the mountain species. The inferred tree suggests that the differentiation of this genus in the Palearctic Region was closely related to the gradual uplifting of the Tibet (Qinghai-Xizang) Plateau, as hypothesized previously, and that vicariance might have played a major role in the differentiation of this genus on the Plateau, On the other hand, the North American species, O. princeps, is most likely a dispersal event, which might have happened during the Pliocene through the opening of the Bering Strait. The phylogenetic relationships within the shrub-steppe group are worth noting in that instead of a monophyletic shrub-dwelling group, shrub dwellers and steppe dwellers are intermingled with each other. Moreover, the sequence divergence within the sister tars of one steppe? dweller and one shrub dweller is very low. These findings support the hypothesis that pikes have entered the steppe environment several times and that morphological similarities within steppe dwellers were due to convergent evolution. (C) 2000 Academic Press.

Genetic diversity and taxonomic implication of Cordyceps sinensis as revealed by RAPD markers

Random amplified polymorphic DNA (RAPD) markers are used to investigate genetic variation and evolutionary relationships of 29 samples of Cordyceps sinensis from different geographical populations on the Qinghai-Tibet plateau. Out of 137 RAPD bands scored, 100 are polymorphic. A correlation is revealed between geographical distance and genetic distance. The molecular phylogenetic tree suggests that the 29 samples are divided into three notable clusters, corresponding to the geographical populations, i.e., the north population (NP), middle population (MP), and south population (SP). The NP consists of 7 northern samples from Menyuan, Maqu, and Luqu, the MP consists of 8 samples from Yushu and Chengduo, and the SP consists of 14 samples from Byma Snow Mountain, Renzhi Snow Moutain, Chongcaoxiwa, and Dacaodi. It is demonstrated that extensive genetic diversity is found among different geographical populations of C. sinensis. The genetic diversity pattern of C. sinensis may be caused by the founder effects. The taxonomic status of NP, MP, and SP populations should be that they are different subspecies rather than different species.

DEVELOPMENT OF MICROSATELLITE LOCI FOR PINUS KORAIENSIS (PINACEAE)

Premise of the study: Microsatellite markers were developed for Pinus koraiensis to characterize its genetic diversity and understand its population structure. Methods and Results: Using the Fast Isolation by AFLP of Sequences COntaining (FIASCO) Repeats protocol, 20 primer sets were developed in Chinese populations of P. koraiensis. Three of the markers showed polymorphism with two alleles per locus when assessed in a sample of two populations of P. koraiensis from the Changbai Mountain in the Jilin Province of China. Five and three loci were successfully amplified in P. taiwanensis and P. massoniana, respectively. The amplification size of these loci matches those in P. koraiensis. Conclusions: These markers may be useful for further investigation of population genetics of P. koraiensis.