DEVELOPMENT OF MICROSATELLITE LOCI FOR PINUS KORAIENSIS (PINACEAE)

Premise of the study: Microsatellite markers were developed for Pinus koraiensis to characterize its genetic diversity and understand its population structure. Methods and Results: Using the Fast Isolation by AFLP of Sequences COntaining (FIASCO) Repeats protocol, 20 primer sets were developed in Chinese populations of P. koraiensis. Three of the markers showed polymorphism with two alleles per locus when assessed in a sample of two populations of P. koraiensis from the Changbai Mountain in the Jilin Province of China. Five and three loci were successfully amplified in P. taiwanensis and P. massoniana, respectively. The amplification size of these loci matches those in P. koraiensis. Conclusions: These markers may be useful for further investigation of population genetics of P. koraiensis.

This project was supported financially by NSFC (No. 30830115 and 20802082), funds from the Chinese Academy of Sciences ("Xibuzhiguang" to W.-L.X. and no, KSCX1-YW-R-24), 973 Program (nos. 2009CB522303, 2009CB522306, and 2009CB940900), the Scientific and Technological Projects of China (2009ZX09501-029), the Young Academic and Technical Leader Rising Foundation of Yunnan Province (2006PY01-47), and the Natural Science Foundation of Yunnan Province (2005XY04 and 2006B0042Q).