Enlarged tracheoesophageal puncture: A systematic review and retrospective analysis

Background. An enlarged tracheoesophageal puncture (TEP) results in aspiration around the voice prosthesis (VP) and may lead to pneumonia. The aims of this research were: (1) to conduct a systematic review and meta-analysis on enlarged TEP; (2) to analyze preoperative, perioperative, and postoperative risk factors for enlarged TEP; and (3) to evaluate control of leakage around the VP using conservative treatments and adverse events in patients with enlarged TEP.^ Methods. A systematic review was conducted (1978-2008). A summary risk estimate was calculated using a random-effects meta-analysis model. A retrospective cohort study was completed. Patients who underwent total laryngectomy and TEP at The University of Texas M. D. Anderson Cancer Center (MDACC) were included. Multiple logistic regression methods were used to assess risk factors for enlargement. Descriptive and bivariate statistics were calculated to evaluate outcomes and adverse events. Results: Twenty-seven manuscripts were included in the systematic review. The summary risk estimate of enlarged TEP/leakage around the VP was 7.2% (95% CI: 4.8%-9.6%). Temporary VP removal and TEP-site injections were the most commonly reported treatments. Neither prosthetic diameter (p=0.076) nor timing of TEP (p=0.297) significantly increased risk of enlargement per stratified analyses of published outcomes. The cumulative incidence of enlarged TEP was 18.6% (36/194, 95% CI: 13.0%-24.1%) in the MDACC cohort. Enlarged TEP occurred exclusively in irradiated patients. Adjusting for length of follow-up and timing of TEP, advanced nodal disease (ORadjusted: 4.3, 95% CI: 1.0-19.1), stricture (ORadjusted : 3.2, 95% CI: 1.2-8.6), and locoregional recurrence/distant metastasis after laryngectomy (ORadjusted: 6.2, 95% CI: 2.3-16.4) increased risk of enlarged TEP. At last follow-up, conservative methods controlled leakage around the VP in 81% (29/36) of patients. Unresolved leakage was associated with recurrent cancer (p=0.081) and TEP-site irregularity (p=0.003). Relative to those without enlargement, enlarged TEP patients had significantly higher risk of pneumonia (RR: 3.4, 95% CI: 1.9-6.2).^ Conclusions. These data establish that enlarged TEP poses serious health risks, and provide insight into medical and oncologic factors that may contribute to development of this complication. In addition, this research supports the use of conservative treatments to address leakage after enlarged TEP in lieu of complete TEP closure.^

Modeling Techniques for the High-Resolution Interpretation of Cryo-Electron Microscopy Reconstructions

Essential biological processes are governed by organized, dynamic interactions between multiple biomolecular systems. Complexes are thus formed to enable the biological function and get dissembled as the process is completed. Examples of such processes include the translation of the messenger RNA into protein by the ribosome, the folding of proteins by chaperonins or the entry of viruses in host cells. Understanding these fundamental processes by characterizing the molecular mechanisms that enable then, would allow the (better) design of therapies and drugs. Such molecular mechanisms may be revealed trough the structural elucidation of the biomolecular assemblies at the core of these processes. Various experimental techniques may be applied to investigate the molecular architecture of biomolecular assemblies. High-resolution techniques, such as X-ray crystallography, may solve the atomic structure of the system, but are typically constrained to biomolecules of reduced flexibility and dimensions. In particular, X-ray crystallography requires the sample to form a three dimensional (3D) crystal lattice which is technically di‑cult, if not impossible, to obtain, especially for large, dynamic systems. Often these techniques solve the structure of the different constituent components within the assembly, but encounter difficulties when investigating the entire system. On the other hand, imaging techniques, such as cryo-electron microscopy (cryo-EM), are able to depict large systems in near-native environment, without requiring the formation of crystals. The structures solved by cryo-EM cover a wide range of resolutions, from very low level of detail where only the overall shape of the system is visible, to high-resolution that approach, but not yet reach, atomic level of detail. In this dissertation, several modeling methods are introduced to either integrate cryo-EM datasets with structural data from X-ray crystallography, or to directly interpret the cryo-EM reconstruction. Such computational techniques were developed with the goal of creating an atomic model for the cryo-EM data. The low-resolution reconstructions lack the level of detail to permit a direct atomic interpretation, i.e. one cannot reliably locate the atoms or amino-acid residues within the structure obtained by cryo-EM. Thereby one needs to consider additional information, for example, structural data from other sources such as X-ray crystallography, in order to enable such a high-resolution interpretation. Modeling techniques are thus developed to integrate the structural data from the different biophysical sources, examples including the work described in the manuscript I and II of this dissertation. At intermediate and high-resolution, cryo-EM reconstructions depict consistent 3D folds such as tubular features which in general correspond to alpha-helices. Such features can be annotated and later on used to build the atomic model of the system, see manuscript III as alternative. Three manuscripts are presented as part of the PhD dissertation, each introducing a computational technique that facilitates the interpretation of cryo-EM reconstructions. The first manuscript is an application paper that describes a heuristics to generate the atomic model for the protein envelope of the Rift Valley fever virus. The second manuscript introduces the evolutionary tabu search strategies to enable the integration of multiple component atomic structures with the cryo-EM map of their assembly. Finally, the third manuscript develops further the latter technique and apply it to annotate consistent 3D patterns in intermediate-resolution cryo-EM reconstructions. The first manuscript, titled An assembly model for Rift Valley fever virus, was submitted for publication in the Journal of Molecular Biology. The cryo-EM structure of the Rift Valley fever virus was previously solved at 27Å-resolution by Dr. Freiberg and collaborators. Such reconstruction shows the overall shape of the virus envelope, yet the reduced level of detail prevents the direct atomic interpretation. High-resolution structures are not yet available for the entire virus nor for the two different component glycoproteins that form its envelope. However, homology models may be generated for these glycoproteins based on similar structures that are available at atomic resolutions. The manuscript presents the steps required to identify an atomic model of the entire virus envelope, based on the low-resolution cryo-EM map of the envelope and the homology models of the two glycoproteins. Starting with the results of the exhaustive search to place the two glycoproteins, the model is built iterative by running multiple multi-body refinements to hierarchically generate models for the different regions of the envelope. The generated atomic model is supported by prior knowledge regarding virus biology and contains valuable information about the molecular architecture of the system. It provides the basis for further investigations seeking to reveal different processes in which the virus is involved such as assembly or fusion. The second manuscript was recently published in the of Journal of Structural Biology (doi:10.1016/j.jsb.2009.12.028) under the title Evolutionary tabu search strategies for the simultaneous registration of multiple atomic structures in cryo-EM reconstructions. This manuscript introduces the evolutionary tabu search strategies applied to enable a multi-body registration. This technique is a hybrid approach that combines a genetic algorithm with a tabu search strategy to promote the proper exploration of the high-dimensional search space. Similar to the Rift Valley fever virus, it is common that the structure of a large multi-component assembly is available at low-resolution from cryo-EM, while high-resolution structures are solved for the different components but lack for the entire system. Evolutionary tabu search strategies enable the building of an atomic model for the entire system by considering simultaneously the different components. Such registration indirectly introduces spatial constrains as all components need to be placed within the assembly, enabling the proper docked in the low-resolution map of the entire assembly. Along with the method description, the manuscript covers the validation, presenting the benefit of the technique in both synthetic and experimental test cases. Such approach successfully docked multiple components up to resolutions of 40Å. The third manuscript is entitled Evolutionary Bidirectional Expansion for the Annotation of Alpha Helices in Electron Cryo-Microscopy Reconstructions and was submitted for publication in the Journal of Structural Biology. The modeling approach described in this manuscript applies the evolutionary tabu search strategies in combination with the bidirectional expansion to annotate secondary structure elements in intermediate resolution cryo-EM reconstructions. In particular, secondary structure elements such as alpha helices show consistent patterns in cryo-EM data, and are visible as rod-like patterns of high density. The evolutionary tabu search strategy is applied to identify the placement of the different alpha helices, while the bidirectional expansion characterizes their length and curvature. The manuscript presents the validation of the approach at resolutions ranging between 6 and 14Å, a level of detail where alpha helices are visible. Up to resolution of 12 Å, the method measures sensitivities between 70-100% as estimated in experimental test cases, i.e. 70-100% of the alpha-helices were correctly predicted in an automatic manner in the experimental data. The three manuscripts presented in this PhD dissertation cover different computation methods for the integration and interpretation of cryo-EM reconstructions. The methods were developed in the molecular modeling software Sculptor (http://sculptor.biomachina.org) and are available for the scientific community interested in the multi-resolution modeling of cryo-EM data. The work spans a wide range of resolution covering multi-body refinement and registration at low-resolution along with annotation of consistent patterns at high-resolution. Such methods are essential for the modeling of cryo-EM data, and may be applied in other fields where similar spatial problems are encountered, such as medical imaging.

The SanaViz: Human Centered Geovisualization to facilitate visual exploration of Public Health data

These three manuscripts are presented as a PhD dissertation for the study of using GeoVis application to evaluate telehealth programs. The primary reason of this research was to understand how the GeoVis applications can be designed and developed using combined approaches of HC approach and cognitive fit theory and in terms utilized to evaluate telehealth program in Brazil. First manuscript The first manuscript in this dissertation presented a background about the use of GeoVisualization to facilitate visual exploration of public health data. The manuscript covered the existing challenges that were associated with an adoption of existing GeoVis applications. The manuscript combines the principles of Human Centered approach and Cognitive Fit Theory and a framework using a combination of these approaches is developed that lays the foundation of this research. The framework is then utilized to propose the design, development and evaluation of “the SanaViz” to evaluate telehealth data in Brazil, as a proof of concept. Second manuscript The second manuscript is a methods paper that describes the approaches that can be employed to design and develop “the SanaViz” based on the proposed framework. By defining the various elements of the HC approach and CFT, a mixed methods approach is utilized for the card sorting and sketching techniques. A representative sample of 20 study participants currently involved in the telehealth program at the NUTES telehealth center at UFPE, Recife, Brazil was enrolled. The findings of this manuscript helped us understand the needs of the diverse group of telehealth users, the tasks that they perform and helped us determine the essential features that might be necessary to be included in the proposed GeoVis application “the SanaViz”. Third manuscript The third manuscript involved mix- methods approach to compare the effectiveness and usefulness of the HC GeoVis application “the SanaViz” against a conventional GeoVis application “Instant Atlas”. The same group of 20 study participants who had earlier participated during Aim 2 was enrolled and a combination of quantitative and qualitative assessments was done. Effectiveness was gauged by the time that the participants took to complete the tasks using both the GeoVis applications, the ease with which they completed the tasks and the number of attempts that were taken to complete each task. Usefulness was assessed by System Usability Scale (SUS), a validated questionnaire tested in prior studies. In-depth interviews were conducted to gather opinions about both the GeoVis applications. This manuscript helped us in the demonstration of the usefulness and effectiveness of HC GeoVis applications to facilitate visual exploration of telehealth data, as a proof of concept. Together, these three manuscripts represent challenges of combining principles of Human Centered approach, Cognitive Fit Theory to design and develop GeoVis applications as a method to evaluate Telehealth data. To our knowledge, this is the first study to explore the usefulness and effectiveness of GeoVis to facilitate visual exploration of telehealth data. The results of the research enabled us to develop a framework for the design and development of GeoVis applications related to the areas of public health and especially telehealth. The results of our study showed that the varied users were involved with the telehealth program and the tasks that they performed. Further it enabled us to identify the components that might be essential to be included in these GeoVis applications. The results of our research answered the following questions; (a) Telehealth users vary in their level of understanding about GeoVis (b) Interaction features such as zooming, sorting, and linking and multiple views and representation features such as bar chart and choropleth maps were considered the most essential features of the GeoVis applications. (c) Comparing and sorting were two important tasks that the telehealth users would perform for exploratory data analysis. (d) A HC GeoVis prototype application is more effective and useful for exploration of telehealth data than a conventional GeoVis application. Future studies should be done to incorporate the proposed HC GeoVis framework to enable comprehensive assessment of the users and the tasks they perform to identify the features that might be necessary to be a part of the GeoVis applications. The results of this study demonstrate a novel approach to comprehensively and systematically enhance the evaluation of telehealth programs using the proposed GeoVis Framework.

Proceedings of the Twenty-Fifth Annual Biochemical Engineering Symposium

The Annual Biochemical Engineering Symposium Series started in 1970 when Professors Larry E. Erickson (Kansas State University) and Peter J. Reilly (then with University of Nebraska-Lincoln) got together in Manhattan, KS along with their students for a half-day powwow and technical presentation by their students. Ever since then, it has been a forum for Biochemical Engineering students in the heartland of USA to present their research to their colleagues in the form of talks and posters. The institutions actively involved with this annual symposium include Colorado State University, Kansas State University, Iowa State University, University of Colorado, University of Kansas, University of Missouri-Columbia, and University of Oklahoma. The University of lowa and University of Nebraska-Lincoln have also participated in the conference in recent years. The host institutions for the different symposia have been: Kansas State University (1, 3, 5, 9, 12, 16, 20), Iowa State University (6, 7, 10, 13, 17, 22), University of Missouri-Columbia (8, 14, 19, 25), Colorado State University (II, 15, 21), University of Colorado (18, 24), University of Nebraska-Lincoln (2, 4), University of Oklahoma (23). The next symposium will be held at Kansas State University. Proceedings of the Symposium are edited by faculty of the host institution and include manuscripts written and submitted by the presenters (students). These often include works-in-progress and final publication usually takes place in refereed journals. ContentsPatrick C. Gilcrease and Vincent G. Murphy, Colorado State University. Use of 2,4,6-Trinitrotoluene (TNT) As A Nitrogen Source By A Pseudomonas florescens Species Under Aerobic Conditions. Marulidharan Narayanan, Lawrence C. Davis, and Larry E. Erickson, Kansas State University. Biodegradation Studies of Chlorinated Organic Pollutants in a Chamber in the Presence of Alfalfa Plants. S.K. Santharam, L.E. Erickson, and L.T. Fan, Kansas State University.Surfactant-Enhanced Remediation of a Non-Aqueous Phase Contaminant in Soil. Barry Vant-Hull, Larry Gold, and Robert H. Davis, University of Colorado.The Binding of T7 RNA Polymerase to Double-Stranded RNA. Jeffrey A. Kern and Robert H. Davis, University of Colorado.Improvement of RNA Transcription Yield Using a Fed-Batch Enzyme Reactor. G. Szakacs, M. Pecs, J. Sipocz, I. Kaszas, S.R. Deecker, J.C. Linden, R.P. Tengerdy, Colorado State University.Bioprocessing of Sweet Sorghum With In Situ Produced Enzymes. Brad Forlow and Matthias Nollert, University of Oklahoma.The Effect of Shear Stress ad P-selectin Site Density on the Rolling Velocity of White Blood Cells. Martin C. Heller and Theodore W. Randolph, University of Colorado.The Effects of Plyethylene Glycol and Dextran on the Lyophilization of Human Hemoglobin. LaToya S. Jones and Theodore W. Randolph, University of Colorado.Purification of Recombinant Hepatitis B Vaccine: Effect of Virus/Surfactant Interactions. Ching-Yuan Lee, Michael G. Sportiello, Stephen Cape, Sean Ferree, Paul Todd, Craig E. Kundrot, and Cindy Barnes, University of Colorado.Application of Osmotic Dewatering to the Crystallization of Oligonucleotides for Crystallography. Xueou Deng, L.E. Erickson, and D.Y.C. Fung, Kansas State University.Production of Protein-Rich Beverages from Cheese Whey and Soybean by rapid Hydration Hydrothermal Cooking. Pedro M. Coutinho, Michael K. Dowd, and Peter J. Reilly, Iowa State University.Automated Docking of Glucoamylase Substrates and Inhibitors. J. Johansson and R.K. Bajpai, University of Missouri.Adsorption of Albumin on Polymeric Microporous Membranes.

Un nuevo viaje de Teseo : De la Antigüedad a la Edad Media y al Renacimiento inglés

El presente trabajo propone explorar el modo cómo la literatura inglesa se apropia de la figura del héroe ático por antonomasia, Teseo, prefiriendo a las historias relacionadas con la aventura aquellas que lo muestran como figura garante del orden y de la sabiduría. Proponemos rastrear el modo cómo el héroe es presentado en el "Cuento del caballero" de los Canterbury Tales, representando no sólo el discurso patriarcal sino y, al mismo tiempo, quien desata las contiendas y quien las re-ordena. En este sentido, la figura del cuento- épico en el sentido bajtiniano del término- testimonia el discurso del orden, donde el varón es el eje vertebrador de la sociedad civil y familiar. Shakespeare, que había tomado contacto con las fuentes clásicas y medievales-tanto italianas como inglesas- incorpora en su Sueño de una noche de verano a un nuevo Teseo, que no sólo discurre acerca de la imaginación sino que también retoma la idea de orden a partir de la proclama de que es necesario someter las trampas de la imaginación a la prueba de la "fría razón". A partir de estos dos ejemplos, trataremos de reflexionar el modo cómo la literatura inglesa se apropió de estos mitos clásicos

Troya historiada en The Book of the Duchess y The House of Fame

En este trabajo propongo analizar The Book of the Duchess y The House of Fame a partir de los problemas que introduce la presencia de la historia troyana. El famoso relato está invocado a través de escenas pintadas en paredes o en ventanales, presentados como escenografía. No están incluidos para añadirles nuevas interpretaciones, ni para completar faltantes de información o conciliar versiones divergentes, sino que son utilizados para capitalizar sus resonancias como objeto cultural, incorporado a la memoria social, frente al cual Chaucer construye un universo de sentido para su propia poesía e indaga en los principios que autorizan su texto.

La creación del espacio poético en Momento de Simetría (1973) de Arturo Carrera y en sus manuscritos : Una constante formación de la materia escrituaria

El trabajo explora, desde los aportes de la crítica genética y literaria, la construcción del espacio poético y sus espacios creativos de escritura. Para ello se cuenta con manuscritos del poeta argentino Arturo Carrera, pertenecientes a su segunda obra édita, Momento de simetría [1973]. Partiendo de una referencia astronómica existente en el poemario se revisa lo dicho por la crítica literaria argentina sobre la obra del autor así como también la posibilidad de leer el mapa poético y sus manuscritos como imágenes estéticas en suspensión. Por este motivo, el trabajo cuenta además con un Anexo (en CD) con las imágenes digitales del Archivo Momento de simetría y sus correspondientes transcripciones, a fin de que el lector pueda ir y venir del texto a las imágenes -y viceversa- y construir dicho recorrido

Los manuscritos de El yo y el ello : Una relectura del Icc

Indagamos, a partir de los manuscritos inéditos de Das Ich und das Es, los puntos eminentes instaurados por la lectura de sus tres versiones: borrador, copia en limpio y texto publicado, en el momento en que surge una disimetría entre lo reprimido-icc y un Icc no-todo reprimido. Estos documentos revelan una transcripción 'casi' directa de las formulaciones freudianas en un estado naciente que llevan la marca de pensamientos urgidos por lo real del psicoanálisis, cuando todavía no está presente el tiempo de hacerse comprender en el contexto de su obra. Y es, justamente, en esta condición de inicial que el texto manuscrito ofrece una novedosa redefinición del Icc. Tal como señala en la Introducción las cuestiones que interroga en El yo y el ello retoman los pensamientos iniciados en su escrito de 1920 pero sin seguir la elaboración teórica que llama 'especulación analítica', aunque a último momento incluya sus conclusiones. Esta relectura interroga el encuentro de la pulsión de muerte con la segunda tópica, es decir, con ese material Icc que permanece no-reconocido. Y, a partir de 1924, establece de que manera el supuesto de la pulsión de muerte ilumina la discontinuidad existente ente icc e Icc [ello]. El cruce entre la hipótesis especulativa y un masoquismo erógeno primario produce un giro y da entrada a la cura misma como satisfacción sustitutiva. En síntesis, la lectura de los textos de El yo y el ello contando, por primera vez, con las tres versiones y sus dos anexos, introducen, con la disimilitud icc e Icc, un cambio de pregunta; anticipan la hendidura o Spaltung del sujeto, con ese objeto [el yo-cuerpo] que Freud no termina de construir; y anuncian que el Icc es lo que se funda de la huella de lo no-reconocido, que clama por ese mismo campo heterogéneo que obligaba en 1920 a tomar en consideración un más allá del principio de placer que divide el espacio euclidiano dejando asomar también su carácter disímil, asimétrico. Así, en ciertos momentos privilegiados de un análisis, vía trabajo del sueño, se produce la activación de los restos de lo visto y de lo oído, es decir, de un saber hecho de sedimentos, de los excedentes traumáticos del tesoro de palabras, para cada cual, de su lengua materna, cuando dicha lengua se separa del lenguaje produciendo un Icc no-todo reprimido que bordea el agujero de lo no-reconocido, es decir, de su punto de fracaso mismo: la imposibilidad con que el sexo se inscribe en el inconsciente

Descripción de colecciones de manuscritos : Una propuesta articulada desde la bibliotecología y la archivística

Los documentos manuscritos constituyen fuentes de información primaria, tanto para estudios históricos como para estudios literarios del área de la filología y la crítica genética. La descripción y difusión de este patrimonio constituye una prioridad para las bibliotecas que son depositarias del mismo, dado el creciente interés observado por este tipo de documentos. En pos de contribuir a este objetivo, se brinda un panorama de la normativa vigente aplicable a la descripción de manuscritos. Se enfatizan los beneficios de la descripción normalizada y del empleo de esquemas de metadatos específicos para la difusión de este tipo de material en Internet y se propone su integración a las prácticas bibliotecológicas corrientes

Huida de una voz en Cae la noche tropical, de Manuel Puig

El análisis literario permite distinguir la presencia de una voz femenina por toda la obra de Manuel Puig. El personaje que la expresa en Cae la noche tropical es Nidia. Desde el enfoque de la crítica genética fue posible observar en los materiales escriturales la configuración de esa voz y su relación con los lugares comunes -rasgo ya señalado como recurrente en la poética de este novelista por la crítica literaria-, así como el proceso por el cual logra fugarse del rol esperado y el cliché

Ricardo Piglia, una poética de la reescritura

El trabajo constituye una primera aproximación a los manuscritos de Prisión perpetua, la consideración de un archivo futuro y las reflexiones teóricas y críticas que suscita ese acontecimiento. Se postula, además, la figuración de una poética basada en el mecanismo de la reescritura y en especial de un tipo de reescritura que denominamos aquí la 'estrategia de Menard', donde las recontextualizaciones de la propia obra conforman un sistema de rentabilidad narrativa que confluye en la multiplicación en el nivel de la producción textual

La representación de la prueba caballeresca como aventura familiar en tres imágenes del Ms.P del Libro del cavallero Zifar

El manuscrito P (Biblioteca Nacional de Francia) del Libro del cavallero Zifar es un códice de fines del siglo XV que, debido a las cerca de 240 ilustraciones que contiene, permite apreciar la dinámica particular de producción y recepción de aquellos manuscritos medievales en los que se conjugan el texto y la imagen como configuradores de sentido. A través de la focalización de un grupo de ilustraciones miniadas que dan cuenta de la dinámica de la aventura caballeresca, centrada tanto en Zifar como en su familia, el presente trabajo postula la relación de esas imágenes con el texto en una retórica deudora tanto de la redacción inicial del texto como de su recepción más de un siglo y medio después

¿Dónde sucede la literatura? Libro, manuscrito y archivo en Manuel Puig y Mario Bellatin

El artículo problematiza la noción de literatura ligada a la cultura del libro, a través del enfoque geneticista y de las obras de dos autores. Manuel Puig que, desde un proyecto creador ligado a la cultura del libro, abrió las fronteras de la intertextualidad hacia la intermedialidad; y Mario Bellatin, cuya obra excede la noción de libro cerrado en tanto cada uno de sus libros reformula y reconstituye fragmentos textuales que pueden aparecer en otros libros. La noción de archivo, vista como máquina social de lectura y de construcción de memoria, puede ser útil para comprender las nuevas propuestas estéticas y revisar otras más tradicionales

Márgenes y zonas de densidad : Acerca de un manuscrito de Leónidas Lamborghini

El presente artículo indaga acerca del proceso de creación de Leónidas Lamborghini a partir del concepto de margen, entendido como diferencia, valor agregado y lugar de la apropiación. A lo largo del trabajo se muestra la existencia en el proceso escritural 'a partir de textos ajenos y propios' de un espacio de creación en el que Lamborghini distorsiona, subvierte, produce plusvalor (en la apropiación) que vuelca en su propia narrativa. Asimismo y a partir de la consulta, el relevamiento y la descripción de los pre-textos de Trento se muestra cómo una parte fundamental de la génesis de la escritura de Lamborghini se encuentra en esas glosas de los márgenes de sus manuscritos

Conocer el Nuevo Mundo : Referencialidad en las ilustraciones de Fernández de Oviedo

Las ilustraciones realizadas por el cronista oficial de Indias Gonzalo Fernández de Oviedo para sus apartados sobre la flora americana son las primeras de este tipo aparecidas mundialmente en una edición impresa. Entre los paradigmas del saber libresco medieval y el de la creciente valoración del testimonio de vista, el Veedor del Oro en el Nuevo Mundo, escritor y dibujante, recurre a las herramientas del lenguaje visual para, por un lado, ofrecer pruebas fehacientes de la naturaleza que estaba presenciando y, por el otro, para aprehenderla mediante las formas de discernimiento del discurso imperial. Sirviéndonos de un análisis de estas imágenes que Oviedo incorporó a sus impresos y manuscritos sobre las Indias, en este trabajo, demostramos cómo en ellas se concentran múltiples referencias sorprendentemente significativas en su conjunto: referencias a la realidad americana por mímesis y semejanza, referencias a los esquemas familiares de las tradiciones pictóricas de Occidente y, por último, a las jerarquías imperiales cristianas. Todo este complejo referencial se encuentra finalmente al servicio de la expansión imperial