Utilisation of bacterial (Rubrivivax gelatinosus) biomass for egg yolk pigmentation

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Effect of different tannery sludge compost amendment rates on growth, biomass accumulation and yield responses of Capsicum plants

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Soil microbial biomass and organic matter fractions during transition from conventional to organic farming systems

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Identification of a molecular marker linked to apomixis in Brachiaria humidicola (Poaceae)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Evaluation of 316L Stainless Steel Corrosion Resistance in Solution Simulating the Acid Hydrolysis of Biomass

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Application of dissociation curve analysis to radiation hybrid panel marker scoring: generation of a map of river buffalo (B-bubalis) chromosome 20

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Culture of Candida in vinasse and molasses: Effect of acid and salt addition on biomass and raw protein production

Species of the genera Candida grown in vinasse and molasses were studied under the following conditions: agitation of containers, pH 4.6, culture time of 24 hours at 30°C. The greatest biomass production of C. krusei grown in vinasse was obtained with the addition of 0.1% H3PO4, and of C. guilliermondii and C. utilis with the addition 0.02% urea plus 0.03% H3PO4. Protein levels near 50% were found in C. utilis in vinasse supplemented either with molasses, with 0.05% MgSO4, or with 0.02% urea plus 0.03% H3PO4. © 1982 Springer-Verlag.

Antibody response to the 43 kDa glycoprotein of Paracoccidioides brasiliensis as a marker for the evaluation of patients under treatment

Sera of patients with paracoccidioidomycosis contained IgG-, IgA-, and IgM-specific antibodies to a 43 kDa antigen contained in the filtrate of a culture of Paracoccidioides brasiliensis. IgG- and IgA-specific antibodies were present in all observed patients. The IgM response was more frequent in acute cases, and the mean titers of IgG- and IgM-specific antibodies were higher in the acute forms. By the fourth month of chemotherapy, there was a decay of IgG, IgA, and IgM antibody titers to this antigen in acute cases, correlating with clinical improvement. The detection of IgG and IgA antibodies and the sequential determination of antibodies to the 43 kDa glycoprotein may be useful tools for serodiagnosis and evaluation of therapeutic efficacy.

Influence of fertilizer on coumarin content and biomass production in Mikania glomerata sprengel

Mikania glomerata Sprengel leaf extracts have been used in cough syrup formulations due to the presence of active coumarin derivatives. Yields of such natural compounds in cultivated plants can be affected by several nutritional factors. To determine the effect of fertilization on biomass productivity, organ proportion and coumarin content, Mikania glomerata plants were submitted during one year to treatments with organic (humus or manure) and inorganic (different levels of nitrogen) fertilizers. Coumarin concentration was raised by organic fertilization, inorganic nutrients induced increased phytomass (stem and leaf) yield, however no fertilized plants showed enhanced leaf production.

Biomass production and essential oil yield in Ageratum conyzoides L. - Asteraceae in different stages of development

An experiment was carried out at Sâo Manuel Experimental Farm, FCA-UNESP, Botucatu-SP, in 1994 and 1995, aiming to determine the biomass production and essential oil yield of mentrasto (Ageratum conyzoides L. - Asteraceae) in different stages of development. The statistical design used was a complete randomized block with 8 replications and 3 treatments (pre-flowering, flowering and post-flowering). The spacing was 70 cm between rows and 50 cm between plants, with 70 plants/parcel. The seedlings were transplanted 40 days after sowing. After harvesting, the fragments (roots, stems, leaves, flowers) and whole plants were washed, separated and weighted. The extractions of essential oil were done by hydrodistillation, using Clevenger apparatus, for two hours. There were statistic differences (Tukey 5%) in fresh weight of all vegetal parts w'hen harvested in different development stages. About essential oil yield, statistic differences were not verified among the treatments.

Feeding ecology, density and biomass of the freshwater turtle, Phrynops geoffroanus, inhabiting a polluted urban river in south-eastern Brazil

This study examines the ecology of a population of Geoffroy's side-necked turtle Phrynops geoffroanus inhabiting a polluted urban river in Ribeirão Preto city, São Paulo state, south-eastern Brazil. Adult turtles fed mainly on Chironomidae larvae and pupae (Chironomus cf. plumosus, 100% of occurrence frequency) and domestic waste, but they also consumed terrestrial items (cockroach, snails) and carrion. Juvenile turtles showed more feeding diversity than the adults and exhibited a trend for predation on Chironomidae pupae, but this is not reflected in resource partitioning. The elevated number of turtles (170-230 turtles/ha of river) and biomass (255-345 kg/ha of river) inhabiting this urban river is probably the result of the abundance of sewage and organic waste produced by humans, the absence of predators, and increased availability of nesting areas. Such factors convert this area into an environment highly advantageous for the survival of Geoffroy's side-necked turtle.

Periphyton on natural substratum in jurumirim reservoir (São Paulo, Brazil): Community biomass and primary productivity

Seasonal variation in the biomass and primary productivity of the periphyton on natural substratum (internodes of Echiiwchloa polystaclya HBK Hitch.) was studied during one year (from August 1993 to July 1994) in a lagoon with permanent connection with a river. We also analysed the relationships between the hydrological regime, climatic conditions and physico-chemical variables of water with the biological compounds of the periphyton. Values of dry mass, ash-free dry mass, chlorophyll a and phaeophytin of periphyton ranged from 0.55±0.24 g m-2 to 7.86±4.93 g m-2; 0.28±0.18 g m-2 to 3.72±2.23 g m-2; 0.57±0.09 mg m-2 to 15.57±4.52 mg m-2; 0.03±0.03 mg m-2 to 4.74±3.46 mg m-2, respectively. The primary productivity of periphytic algae measured by C14 method ranged from 6.45±1.29 mg C m-2 h-1 to 52.88± 7.55 mg C m-2 h-1. The biomass showed a peak in October 1993, February and April 1994. Higher value of primary productivity was recorded in December 1993 and January 1994 and was due to the peculiar light and nutrition conditions during this period. We conclude that biomass and productivity of the community are controlled mainly by hydrological regime (fluctuations of water level). © INTERNATIONAL SCIENTIFIC PUBLICATIONS.

Immunocytochemical study of Ki-67 as a prognostic marker in canine mammary neoplasia

Background: Canine mammary tumors are challenging for clinicians and pathologists because of complex histologic classification, low specificity of cytologic diagnosis, and unpredictable biological behavior. In histologic specimens, expression of tumor proliferation marker Ki-67, a nuclear nonhistone protein, has been shown to have prognostic value for canine mammary tumors and to correlate with malignancy and low survival rates. Objective: The objective of this study was to measure the proliferation index of canine mammary tumors by immunochemical detection of Ki-67 in cytologic specimens and to determine its relationship to clinical and pathologic variables and patient outcome. Methods: Spontaneous mammary tumors from 31 female dogs were surgically excised. Imprint specimens for cytologic evaluation were wet-fixed in ethanol; histologic specimens were prepared routinely. Immunostaining was performed with the PH 177 monoclonal antibody against Ki-67; proliferation index was graded from negative to +++. Dogs were followed for 18 months. Multivariate logistic regression analysis was used to determine correlations between immunocytochemical results, tumor and clinical variables, and patient outcome. Results: Ki-67 proliferation indices in cytologic specimens were significantly lower for nonmalignant tumors than for malignant tumors. High index values of Ki-67 were positively correlated with metastasis, death from neoplasia, low disease-free survival rates, and low overall survival rate. With the exception of 4 specimens for which cellularity was insufficient, positive expression of Ki-67 in cytologic specimens correlated with that of histologic specimens. Conclusions: The prognostic value of the Ki-67 index in canine mammary tumors by using wet-fixed cytology imprint specimens was similar to that observed previously for histologic specimens. Immunocytochemical detection of Ki-67 could improve the accuracy and value of cytology by providing safe and rapid information about malignancy and patient outcome. © 2004 American Society for Veterinary Clinical Pathology.

Study of thresholds to burning in surface grinding process

This work aims at finding out the threshold to burning in surface grinding process. Acoustic emission and electric power signals are acquired from an analog-digital converter and processed through algorithms in order to generate a control signal to inform the operator or interrupt the process in the case of burning occurrence. The thresholds that dictate the situation of burn and non-burn were studied as well as a comparison between the two parameters was carried out. In the experimental work one type of steel (ABNT-1045 annealed) and one type of grinding wheel referred to as TARGA model 3TG80.3-NV were employed. Copyright © 2005 by ABCM.

Diurnal variation of zooplankton biochemical composition and biomass in plankton production tanks

The nutritional value of zooplankton (lipid and phosphorus contents) was analyzed in outdoor, plankton-culture tanks, to monitor the effects of diurnal variation and fertilization. Total lipid contents were significantly higher (average values of about 14% DW) for the treatment fertilized during the sampling week. A significantly highher total lipid concentrations generally coincided with high biomass of Moina sp. (Cladocera) and Thermocyclops sp. nauplii (Copepoda), which is probably related to zooplankton filtration rates. The values tended to be higher during the afternoon and the night. Phosphorus contents in the zooplanktonic population varied from 0.15 to 0.91% DW, with significant differences among the tanks. The zooplankton nutritional quality was greatly influenced by fertilization, which also determined the species composition in the tanks.