Solvent-induced changes on the polarity of the triplet excited state of 2-chlorothioxanthone: From time-resolved absorption and resonance Raman spectroscopies

Solvent polarity has been known to influence the triplet state structure and reactivity. Here, we present our experimental and theoretical study on the effect of solvent on the lowest triplet excited state structure of 2-chlorothioxanthone (CTX). Time-resolved absorption (TA) spectroscopy has been employed to understand the triplet state electronic structure; whereas solvent-induced structural changes have been studied using time-resolved resonance Raman (TR3) spectroscopy. Both the DFT and TD-DFT calculations have been performed in the solution phase employing self-consistent reaction field implicit solvation model to support the experimental data. It has been observed that CO stretching frequencies of the excited triplet state are sensitive to the solvent polarity and increase with the increase in the solvent polarity. Both TA and TR3 studies reveal that specific solvent effect (H-bonding) is more pronounced in comparison to the nonspecific solvent effect. (C) 2013 Elsevier B.V. All rights reserved.

Metabolic effects of sub-chronic ablation of the incretin receptors by daily administration of (Pro(3))GIP and exendin(9-39)amide in obese diabetic (ob/ob) mice

Effects of chemical ablation of the GIP and GLP-1 receptors on metabolic aspects of obesity-diabetes were investigated using the stable receptor antagonists (Pro(3))GIP and exendin(9-39)amide. Ob/ob mice received a daily i.p. injection of saline vehicle, (Pro(3))GIP, exendin(9-39)amide or a combination of both peptides over a 14-day period. Non-fasting plasma glucose levels were significantly (p <0.05) lower in (Pro(3))GIP-treated mice compared to control mice after just 9 days of treatment. (Pro(3))GIP-treated mice also displayed significantly lower plasma glucose concentrations in response to feeding and intraperitoneal administration of either glucose or insulin (p <0.05 to p <0.001). The (Pro(3))GIP-treated group also exhibited significantly (p <0.05) reduced pancreatic insulin content. Acute administration of exendin(9-39) amide immediately prior to re-feeding completely annulled the beneficial effects of sub-chronic (Pro(3))GIP treatment, but non-fasting concentrations of active GLP-1 were unchanged. Combined sub-chronic administration of (Pro(3)GIP) with exendin(9-39)amide revealed no beneficial effects. Similarly, daily administration of exendin(9-39)amide alone had no significant effects on any of the metabolic parameters measured. These studies highlight an important role for GIP in obesity-related forms of diabetes, suggesting the possible involvement of GLP-1 in the beneficial actions of GIP receptor antagonism.

TIME-RESOLVED ABSORPTION, INFRARED, AND RESONANCE RAMAN-SPECTRA OF THE COMPLEXES [RU(X)(R)(CO)(2)(ALPHA-DIIMINE)] (X=HALIDE R=ALKYL) - INFLUENCE OF X ON THE CHARGE-TRANSFER CHARACTER OF THE LOWEST EXCITED-STATE

Nanosecond time-resolved absorption (TA), resonance Raman (TR(3)), and infrared (TRIR) spectra are reported for several complexes [Ru(X)(R)(CO)(2)(alpha-diimine)] (X = Cl, Br, I; R = Me, Et; alpha-diimine = N,N'-diisopropyl-1,4-diaza-1,3-butadiene (iPr-DAB), pyridine-2-carbaldehyde-N-isopropylimine (iPr-PyCa), 2,2'-bipyridine (bpy)). This is the first instance in which the TA, TR(3), and TRIR techniques have been used to probe excited states in the same series of complexes. The TA spectra of the iodide complexes show a transient absorption between 550 and 700 nm, which does not depend on the solvent but shifts to lower energy in the order iPr-DAB > bpy > iPr-PyCa. This band is assigned to an intraligand transition. For the corresponding chloride and bromide complexes this band occurs at higher energy, most probably because of a change of character of the lowest excited state from XLCT to MLCT. The TRIR spectra show an increase in v(CO) (and k(CO)) on promotion to the excited state; however, the shifts Delta v(CO) show a decrease in the order Cl- > Br- > I-. The TR(3) spectra of the excited complexes [Ru(X)(R)(Co)(2)(iPr-DAB)] show v(s)(CN) of the iPr-DAB ligand 50-80 cm(-1) lower in frequency than for the complexes in their ground state. This frequency shift decreases in the order Cl- > Br- > I-, indicating a decrease of CT character of the lowest excited state in this order. However, going from X = Br to I, the effect on Delta v(CO) is much larger than the decrease of Delta v(s)(CN). This different effect on the CO- and CN-stretching frequencies is assigned to a gradual change in character of the lowest excited state from MLCT to XLCT when Cl- is replaced by Br- and I-. This result confirms a similar conclusion derived from previous resonance Raman and emission experiments on these complexes.

Evaluation of a new serological test for the detection of anti-Coxiella and anti-Rickettsia antibodies.

Coxiella burnetii and members of the genus Rickettsia are obligate intracellular bacteria. Since cultivation of these organisms requires dedicated techniques, their diagnosis usually relies on serological or molecular biology methods. Immunofluorescence is considered the gold standard to detect antibody-reactivity towards these organisms. Here, we assessed the performance of a new automated epifluorescence immunoassay (InoDiag) to detect IgM and IgG against C. burnetii, Rickettsia typhi and Rickettsia conorii. Samples were tested with the InoDiag assay. A total of 213 sera were tested, of which 63 samples from Q fever, 20 from spotted fever rickettsiosis, 6 from murine typhus and 124 controls. InoDiag results were compared to micro-immunofluorescence. For acute Q fever, the sensitivity of phase 2 IgG was only of 30% with a cutoff of 1 arbitrary unit (AU). In patients with acute Q fever with positive IF IgM, sensitivity reached 83% with the same cutoff. Sensitivity for chronic Q fever was 100% whereas sensitivity for past Q fever was 65%. Sensitivity for spotted Mediterranean fever and murine typhus were 91% and 100%, respectively. Both assays exhibited a good specificity in control groups, ranging from 79% in sera from patients with unrelated diseases or EBV positivity to 100% in sera from healthy patients. In conclusion, the InoDiag assay exhibits an excellent performance for the diagnosis of chronic Q fever but a very low IgG sensitivity for acute Q fever likely due to low reactivity of phase 2 antigens present on the glass slide. This defect is partially compensated by the detection of IgM. Because it exhibits a good negative predictive value, the InoDiag assay is valuable to rule out a chronic Q fever. For the diagnosis of rickettsial diseases, the sensitivity of the InoDiag method is similar to conventional immunofluorescence.

John Miller letter, December 1, 1812.

John Miller (1774-1862) was a physician and politician who served in the United States Congress as a representative from New York from 1825-1827. He began his medical practice in Washington County, N.Y. in 1798. In 1801 he moved to Fabius, Onondaga County, N.Y. (now Truxton, Cortland County). He served as postmaster at Truxton from 1805-1825, a justice of the peace from 1812-1821 and in the State Assembly in 1817, 1820 and 1845. Archibald McIntire [McIntyre] (1772-1858) was a businessman and politician. He immigrated to the United States with his family and settled in New York City around 1773. He was a member of the New York State Assembly from 1798-1821 (intermittently), in the New York State Senate form 1822-1826 and was New York State Comptroller from 1806-1821.

Análisis crítico de la sociología de las emociones y de la acción estratégica para la comprensión de la campaña de desaprobación a Slobodan Milosevíc realizada por OTPOR, en Serbia, a través de la acción política no violenta en el año 2000

En este trabajo el lector va a encontrar una disertación entre la sociología de las emociones y la acción estratégica de Roger Petersen, con el fin de entender movimientos en masa que actúan bajo la acción política no violenta, como fue el caso de OTPOR en Serbia en el 2000

Docket books and fee book, 1791-1806

Consists of 7 notebooks and 1 account book. Notebooks list cases heard before the Massachusetts Court of Common Pleas for Middlesex and Worcester counties and the Massachusetts Supreme Judicial Court (March 1794-Sept. 1797; Sept. 1800-March 1802; June 1805-September 1806) and before the New Hampshire Court of Common Pleas for Hillsborough and Merrimack counties (March 1798-March 1805). The account book includes cases from Sept. 1791-July 1797.

Harvard College Lottery Book, 1794-1797

The long hardcover account book contains handwritten records of the Harvard College Lottery in the hand of College Treasurer Ebenezer Storer. The volume begins with a transcription of the Massachusetts General Court June 13, 1794 legislation sanctioning the lottery, and a note that the managers of the lottery gave security bonds to the Corporation. The bulk of the volume records the activities of the four classes of the lottery including lists of the individual tickets returned by the managers Benjamin Austin Jr., George R. Minot, Henry Warren, and John Kneeland, and the accounts of prizes drawn and tickets returned. The volume has a table of contents and there is a note pasted onto the third page calculating the sum raised if all tickets had been sold.

Library catalogue notes of Isaac Smith, ca. 1789

Thirteen slips of paper with fragments of handwritten alphabetical lists created by Isaac Smith presumably in his capacity as Harvard Librarian. Most of the entries are surnames or single-word subjects. For example, one slip with "M" entries includes: milway, miracles, miraculous, Mitchell, and Mitchell. Some of the lists have struck-through words or have entries annotated with numbers and the abbreviations "o" and "bk." The verso of one leaf has a brief, undated note regarding the transfer of books between Mr. Hilliard and Mr. Smith.

A book of miscellanies, 1770

A legal commonplace book by kept by Henry Wells of Worcester, Mass. Focuses on such topics as libel of a man to his wife, common recovery in writs and deeds, pleadings, trover, damages and costs, imprisonment, leases, mortgages, covenants, and ejectment. Also contains a number of miscellaneous entries touching on abridgements of law texts, minutes of court proceedings, kings of England, and biblical quotes. Five-page index located at the end of the work.

Plea in the case of Hamilton v. Saunders, 1763

Plea in response to the plaintiff's declaration. Signed: (Charles) Pinckney, defendant's attorney, and John Rutledge (plaintiff's attorney?). Note on verso: Filed 4th May 1763.

Law miscellany. 1782-1788

A lawyer's commonplace book of New Hampshire cases. Contains summaries of cases covering such topics as trespass, witnesses, injuries, bailbonds, and counterfeit money. Possibly kept by Jonathan Sewell.