Quantitative and qualitative studies on the bacterial flora of fresh sardines

Plate counts at R T and 8 C on the skin with muscle and the gut contents of absolutely fresh sardines (Sardinella longiceps) caught off Cochin showed a seasonal variation when sampling was done over a period of 12 months. The counts of the gut contents ran parallel with those of the skin with muscle, but at a higher level of magnitude. Qualitatively, the analysis of 360 strains of bacteria isolated from the skin with muscle and 100 strains from the guts during a year's study revealed a very high preponderance of Gram negative rods, mainly of Achromobacter, Vibrio, and Pseudomonas groups. The percentage of Gram positive organism was very low or nil at times in the ocean fresh sardines.

Quantitative survey of Sargassum along the south-west coast of Ceylon

Sargassum is a brown seaweed from which alginic acid is extracted. A survey was carried out off the south-west coast of Ceylon in December 1963 and January 1964 with the view to determining the quantity of Sargassum available.

Quantitative and qualitative assessment of plankton: some ecological aspect and water quality parameters of the river Meghna, Bangladesh

Investigation on the seasonal distribution and abundance of various major taxa of phyto and zooplankton and the corresponding physico-chemical characteristics were carried out in four selected stations between the latitude 22°35.494N N-23°23.987 N and longitude 90°35.793 E- 90°49.061 E of the Meghna river system, Bangladesh. Drop count method was followed for the qualitative and quantitative analysis of both phyto- and zooplankton. A total of 41 phytoplankton genera belonging to 17 families and 13 zooplankton genera belonging to 11 families were recorded. Zooplankton growth cycle was noticeably less (3.0%) than the phytoplankton abundance almost throughout the study period. Quantity of plankton registered to increase chronologically from the upper to lower stretches of the river. During summer investigation the load of phytoplankton was recorded maximum (11,300-51,850 No/1). Ratio-wise quantitative difference between zoo- and phytoplankton in composition of the total standing crop fluctuated between 1.0:5.5 and 1:1037. Among the phytoplanktonic groups, Chlorophyceae was found to be dominating (95.0%) in all sampling stations. Protococcus, a single genus of Chlorophyceae played a unique role during summer, contributing the highest density of about 74.0%. The pattern of qualitative and quantitative difference of plankton standing crop in different sampling sites can be attributed to the existing physico-chemical characteristics, mainly water temperature, pH and hardness.

The bacteriology of oil sardine (Sardinella longiceps) and mackerel (Rastrelliger kanagurta) caught from tropical waters off Cochin. 1 - Quantitative aspects

The total aerobic viable plate counts (TPCs) of skin, gills and intestine of newly caught oil sardine (Sardinella longiceps) and Indian mackerel ( Rastrelliger kanagurta) at four different temperatures, namely 36 ± 1°C, 28 ± 2°C (RT), 8 ± 1°C and 1 ±1°C, are reported. The total plate count at RT of the skin of oil sardine and Indian mackerel were in the range of l0 super(3) to 10 super(7) and 10 super(4) to 10 super(6) per cm², that of gills in the range of 10 super(5) to 10 super(9) and 10 super(4) to 10 super(8) per g and that intestine in the range of 10 super(5) to 10 sueper(9) and 10 super(5) to 10 super(8) per g respectively. The TPCs were markedly affected by the incubation temperature. Incubation at 28 ± 2°C gave the highest count; at 36 ± 1°C and 8 ± 1°C, the counts decreased by nearly 1-2 log cycles from that at RT. Incubation at 1 ± 1°C registered the lowest count. The peak values for bacterial counts of these fishes occurred at different periods of the year.

DEVELOPMENT OF MICROSATELLITE LOCI FOR PINUS KORAIENSIS (PINACEAE)

Premise of the study: Microsatellite markers were developed for Pinus koraiensis to characterize its genetic diversity and understand its population structure. Methods and Results: Using the Fast Isolation by AFLP of Sequences COntaining (FIASCO) Repeats protocol, 20 primer sets were developed in Chinese populations of P. koraiensis. Three of the markers showed polymorphism with two alleles per locus when assessed in a sample of two populations of P. koraiensis from the Changbai Mountain in the Jilin Province of China. Five and three loci were successfully amplified in P. taiwanensis and P. massoniana, respectively. The amplification size of these loci matches those in P. koraiensis. Conclusions: These markers may be useful for further investigation of population genetics of P. koraiensis.

A quantitative study of Vibrio parahaemolyticus (Sakazaki et al) in Etroplus suratensis (Bloch) and Metapenaeus dobsoni (Miers) from Cochin backwater

Seasonal variation of Vibrio parahaemolyticus in fish (Etroplus sauratensis) and prawn (Metapenaeus dobsoni) was monitored from March 1982 to February 1983. Analyses of total viable count, vibrio-like organisms, V. parahaemolyticus like organisms and V. parahaemolyticus showed that they occur more in prawn than in fish. In a more polluted environment, the counts of V. parahaemolyticus associated with fish were found to be higher than in prawn.

Selection and application of a suitable sampling method for quantitative and qualitative evaluation of lab-lab

Milkfish and prawn pond operation in the Philippines is often associated with lab-lab culture. Lab-lab is a biological complex of blue-green algae, diatoms, bacteria and various animals which form a mat at the bottom of nursery ponds or floating patches along the margins of ponds. This complex is considered the most favorable food of milkfish in brackishwater ponds. Variations in the quantity and quality of lab-lab between and within areas of a 1,000 sq. m. pond was determined over 2 culture periods (6 month duration) and the applicability and suitability of stratified random sampling as a method of sampling lab-lab was evaluated.

Quantitative analysis of land mammal zoogeographical regions in China and adjacent regions

Quantitative analysis of land mammal zoogeographical regions in China and adjacent regions. Zoological Studies 43(1): 142-160. In this paper, our aim was to determine, by means of quantitative analysis, the distribution patterns of the land mammals in China and, adjacent regions using physiographical regions as operative geographical units (OGUs). Based, on the pre-sence or absence of 11 orders, 42 families, 197 genera, and 577 species of land mammals in their zoogeographical regions, which were used as OGUs, we studied the biotic boundary between the Oriental Region (OR) and the Palaearctic Region (PR), as well as subregion boundaries. The boundary's statistical significance was tested by G-test as described by McCoy et al. A significantly strong biotic boundary was found to separate the PR from the OR, and there is a weak biotic boundary in the PR, which divides it into 2 subregions. We concluded that the biotic boundary which separates the PR and OR is a strong boundary. We suggest that the Qinghai-Xizang Plateau should be regarded as a subregion of the PR, which can embody its characteristics of high elevations and a frigid climatic, which is called the Qing-Zang subregion of the PR (QZSP).

A novel method for rapid comparative quantitative analysis of nuclear fuel cycles

One of the greatest obstacles facing the nuclear industry is that of sustainability, both in terms of the finite reserves of uranium ore and the production of highly radiotoxic spent fuel which presents proliferation and environmental hazards. Alternative nuclear technologies have been suggested as a means of delivering enhanced sustainability with proposals including fast reactors, the use of thorium fuel and tiered fuel cycles. The debate as to which is the most appropriate technology continues, with each fuel system and reactor type delivering specific advantages and disadvantages which can be difficult to compare fairly. This paper demonstrates a framework of performance metrics which, coupled with a first-order lumped reactor model to determine nuclide population balances, can be used to quantify the aforementioned pros and cons for a range of different fuel and reactor combinations. The framework includes metrics such as fuel efficiency, spent fuel toxicity and proliferation resistance, and relative cycle performance is analysed through parallel coordinate plots, yielding a quantitative comparison of disparate cycles. © 2011 Elsevier Ltd. All rights reserved.