CCR2 and CCR5 genes polymorphisms in women with cervical lesions from Pernambuco, Northeast Region of Brazil: a case-control study

Polymorphisms in chemokine receptors play an important role in the progression of cervical intraepithelial neoplasia (CIN) to cervical cancer (CC). Our study examined the association of CCR2-64I (rs1799864) and CCR5-Δ32 (rs333) polymorphisms with susceptibility to develop cervical lesion (CIN and CC) in a Brazilian population. The genotyping of 139 women with cervical lesions and 151 women without cervical lesions for the CCR2-64I and CCR5-Δ32 polymorphisms were performed using polymerase chain reaction-restriction fragment length polymorphism. The individuals carrying heterozygous or homozygous genotypes (GA+AA) for CCR2-64I polymorphisms seem to be at lower risk for cervical lesion [odds ratio (OR) = 0.37, p = 0.0008)]. The same was observed for the A allele (OR = 0.39, p = 0.0002), while no association was detected (p > 0.05) with CCR5-Δ32 polymorphism. Regarding the human papillomavirus (HPV) type, patients carrying the CCR2-64I polymorphism were protected against infection by HPV type 16 (OR = 0.35, p = 0.0184). In summary, our study showed a protective effect of CCR2-64I rs1799864 polymorphism against the development of cervical lesions (CIN and CC) and in the susceptibility of HPV 16 infection.

Primary dengue haemorrhagic fever in patients from northeast of Brazil is associated with high levels of interferon-b during acute phase

Dengue is an acute febrile disease caused by the mosquito-borne dengue virus (DENV) that according to clinical manifestations can be classified as asymptomatic, mild or severe dengue. Severe dengue cases have been associated with an unbalanced immune response characterised by an over secretion of inflammatory cytokines. In the present study we measured type I interferon (IFN-I) transcript and circulating levels in primary and secondary DENV infected patients. We observed that dengue fever (DF) and dengue haemorrhagic fever (DHF) patients express IFN-I differently. While DF and DHF patients express interferon-a similarly (52,71 ± 7,40 and 49,05 ± 7,70, respectively), high levels of circulating IFN-b were associated with primary DHF patients. On the other hand, secondary DHF patients were not able to secrete large amounts of IFN-b which in turn may have influenced the high-level of viraemia. Our results suggest that, in patients from our cohort, infection by DENV serotype 3 elicits an innate response characterised by higher levels of IFN-b in the DHF patients with primary infection, which could contribute to control infection evidenced by the low-level of viraemia in these patients. The present findings may contribute to shed light in the role of innate immune response in dengue pathogenesis.

Climatic fluctuations and homogenization of Northeast Brazil using precipitation data.

Before using the basic precipitation data in any agroclimatic study to assess the productivity it is important to check the data series for homogeneity. For this purpose data of 105 locations for the period 1912-1981 over northeast Brazil were used. The preliminary study indicate nonhomogeneity in the time series during 1940's at few locations. The amplitude of variation of time series when taken as 10-year moving average show quite different for different regions. It appears that this amplitude is related to time of onset of effective rains in some extent. There is also great diversity in the fluctuations. They present a great regional diversity. Some diversity. Some of the data in the low latitudes indicate presence of four cycles namely 52, 26, 13 & 6.5. years. The 52-year cycle is also evident in the case of onset of southwest Monsoon over a low latitude zone (Kerala Coast) in India. In the case of south Africa the prominent cycles are 60, 30, 15 & 10 similar situation appears to be present in the higher latitudes of northeast Brazil.

Climate change and impacts on family farming in the North and Northeast of Brazil.

The starting point for this study was the consideration of future climate change scenarios and their uncertainties. The paper presents the global projections from the Intergovernmental Panel on Climate Change (IPCC) Fifth Assessment Report (AR5) and compares them with regional scenarios (downscaling) developed by the Brazilian National Institute for Space Research (Instituto Nacional de Pesquisas Espaciais - INPE), with a focus on two main IPCC scenarios (RCP4.5 and RCP8.5) and two main global models (MIROC and Hadley Centre) for the periods 2011-2040 and 2041-2070. It aims to identify the main trends in terms of changes in temperature and precipitation for the North and Northeast regions of Brazil (more specifically, in the Amazon, semi-arid and cerrado biomes).

A review of maize-beans and maize-cowpea intercrop systems in the semiarid Northeast Brazil.

Fifty-one experiments on maize -beans and 34 on maize-cowpea intercropping systems conducted mostly in semiarid Northeast Brazil were analysed to get an understanding of the performance of these intercrops in terms of their productivity as well as stability. Both the intercrop systems produced higher yields over their respective sole crops under a wide range of agroclimates; the average advantage with maize-beans was 32%, while that from maize-cowpea was 41%. The optimum row proportion for maize-beans was one maize: three beans, requiring 59% of sole crop maize population and 75% sole bean population. In maize-cowpea, alternate rows or one maize: two cowpea arrangement with about 50% of sole maize density and 100% of sole cowpea population seemed to be optimum. The intercrops failed less frequently compared to sole crops to meet specified incomes or yields. Sorghum seemed to be a good alternative to the traditional cereal because of its improved and consistent performance. Future research needs are discussed for further yield improvement in these two intercrop system.s

Water production function of maize for Northeast Brazil.

Estudou-se atraves de um experimento em blocos ao acaso, os efeitos de quatro niveis de nitrogenio, em diferentes condicoes de umidade, sobre os estagios de crescimento, embonecamento, formacao de graos e prdutividade do milho (Zea mays L.) e as relacoes entre a produtividade e os tres primeiros estagios. Os fatores da resposta de producaobaseados na equacao de Doorenbos e Kassam variaram acentuadamente, nao so com os diferentes estatios de crescimento, mas tambem com diferentes niveis de nitrogenio e os diferentes niveis de agua. Assim, esta equacao nao pareceu ser valida para explicar a resposta de produtividade a niveis de agua. Sugeriu-se um equacao linear modificada. Nesta equacao, a intercessao K1 e inclinacao K2 sao os fatores da resposta de producao. Estes fatores para a cultura do milho foram desenvolvidos para todos os quatro estagios de crescimento e nveis de nitrogenio. Pode-se obter uma eficiencia media do uso de agua, em termos de produtividade, de, aproximadamente, 57,5 kg/ha-cm de agua, sendo, contudo, pequeno o incremento, em face dos niveis crescentes de nitrogenio aplicado ate 120 kg/ha. Os coeficientes de cutura (Kc) calculados estao muito abaixo da estimativa da FAO, para todos os niveis de nitrogenio. Por essa razao, deve haver consideravel economica de agua se estes coeficientes forem usados em lugar da estimativa da FAO. A informacao mostradapode imediatamente ser utilizada para turno de irrigacao e para projetos de irrigacao suplementar planejado para as condicoes doe Nordeste do Brasil.

Production of Syrah wines in tropical conditions of Northeast Brazil.

Twenty years ago, in the Northeast Brazil, a tropical semi-arid area, the production of tropical wines from some Vitis vinifera L. cultivars started. In this region, it is possible to harvest two times a year, between April and December, and nowadays Syrah is the most cultivated wine grape. Research works about irrigation rnanagement in Syrah started in 2000 at Brazilian Agricultural Research Corporation (Embrapa). The aim of this paper is to introduce some characteristics about the region where Syrah grapevines is being cultivated under irrigation in Northeast Brazil, and to present some results obtained from field experiments as well as some grape and wine charcteristics already determined.

Climate change and irrigation water requirement at Jaguaribe river basin, semi-arid northeast of Brazil.

Summary: Climate change has a potential to impact rainfall, temperature and air humidity, which have relation to plant evapotranspiration and crop water requirement. The purpose of this research is to assess climate change impacts on irrigation water demand, based on future scenarios derived from the PRECIS (Providing Regional Climates for Impacts Studies), using boundary conditions of the HadCM3 submitted to a dynamic downscaling nested to the Hadley Centre regional circulation model HadRM3P. Monthly time series for average temperature and rainfall were generated for 1961-90 (baseline) and the future (2040). The reference evapotranspiration was estimated using monthly average temperature. Projected climate change impact on irrigation water demand demonstrated to be a result of evapotranspiration and rainfall trend. Impacts were mapped over the target region by using geostatistical methods. An increase of the average crop water needs was estimated to be 18.7% and 22.2% higher for 2040 A2 and B2 scenarios, respectively. Objective ? To analyze the climate change impacts on irrigation water requirements, using downscaling techniques of a climate change model, at the river basin scale. Method: The study area was delimited between 4º39?30? and 5º40?00? South and 37º35?30? and 38º27?00? West. The crop pattern in the target area was characterized, regarding type of irrigated crops, respective areas and cropping schedules, as well as the area and type of irrigation systems adopted. The PRECIS (Providing Regional Climates for Impacts Studies) system (Jones et al., 2004) was used for generating climate predictions for the target area, using the boundary conditions of the Hadley Centre model HadCM3 (Johns et al., 2003). The considered time scale of interest for climate change impacts evaluation was the year of 2040, representing the period of 2025 to 2055. The output data from the climate model was interpolated, considering latitude/longitude, by applying ordinary kriging tools available at a Geographic Information System, in order to produce thematic maps.

Paleoenvironments and Geochemical Signals from the Late Barremian to the Middle Aptian in a Tethyan Marginal Basin, Northeast Spain: Implications for Carbon Sequestration in Restricted Basins

The hallmark of oceanic anoxic event 1a (OAE1a) (early Aptian ~125 Ma) corresponds to worldwide deposition of black shales with total organic carbon (TOC) content > 2% and a d13C positive excursion up to ~5‰. OAE1a has been related to large igneous province volcanism and dissociation of methane hydrates during the Lower Cretaceous. However, the occurrence of atypical, coeval and diachronous organic-rich deposits associated with OAE1a, which are also characterized by positive spikes of the d13C in epicontinental to restricted marine environments of the Tethys Ocean, indicates localized responses decoupled from complex global forcing factors. The present research is a high-resolution, multiproxy approach to assess the paleoenvironmental conditions that led to enhanced carbon sequestration from the late Barremian to the middle Aptian in a restricted, Tethyan marginal basin prior to and during OAE1a. I studied the lower 240 m of the El Pui section, Organyà Basin, Spanish Pyrenees. The basin developed as the result of extensional tectonism linked to the opening of the Atlantic Ocean. At the field scale the section consists of a sequence of alternating beds of cm – m-scale, medium-gray to grayish-black limestones and marlstones with TOC up to ~4%. The results indicate that the lowest 85 m of the section, from latest Barremian –earliest Aptian, characterize a deepening phase of the basin concomitant with sustained riverine flux and intensified primary productivity. These changes induced a shift in the sedimentation pattern and decreased the oxygen levels in the water column through organic matter respiration and limited ventilation of the basin. The upper 155 m comprising the earliest – late-early Aptian document the occurrence of OAE1a and its associated geochemical signatures (TOC up to 3% and a positive shift in d13C of ~5‰). However, a low enrichment of redox-sensitive trace elements indicates that the basin did not achieve anoxic conditions. The results also suggest that a shallower-phase of the basin, coeval with platform progradation, may have increased ventilation of the basin at the same time that heightened sedimentation rates and additional input of organic matter from terrestrial sources increased the burial and preservation rate of TOC in the sediment.

White-faced storm-petrels Pelagodroma marina predated by gulls as biological monitors of plastic pollution in the pelagic subtropical northeast atlantic

Marine plastic pollution is rapidly growing and is a source of major concern. Seabirds often ingest plastic debris and are increasingly used as biological monitors of plastic pollution. However, virtually no studies have assessed plastics in seabirds in the deep subtropical North Atlantic. We investigated whether remains of white-faced storm-petrels (WFSP) present in gull pellets could be used for biomonitoring. We analysed 263 pellets and 79.0% of these contained plastic debris originating in the digestive tract of WFSP. Pellets with no bird prey did not contain plastics. Most debris were fragments (83.6%) with fewer plastic pellets (8.2%). Light-coloured plastics predominated (71.0%) and the most frequent polymer was HDPE (73.0%). Stable isotopes in toe-nails of WFSP containing many versus no plastics did not differ, indicating no individual specialisation leading to differential plastic ingestion. We suggest WFSP in pellets are highly suitable to monitor the little known pelagic subtropical Northeast Atlantic.

Feeding ecology of the deep-sea lanternshark Etmopterus pusillus (Elasmobranchii: Etmopteridae) in the northeast Atlantic

This study provides the first description of the feeding ecology of the smooth lanternshark Etmopterus pusillus based on stomach contents of specimens caught as bycatch in the Algarve (southern Portugal) with bottom trawling and bottom longline. The diet of E. pusillus consists mainly of fish (dry weight (% W)=87.1%; frequency of occurrence (%FO)=28.6%; number (%N)=30.3%), crustaceans (%W=7.7%; %FO=36.7%; %N=3.4%) and cephalopods (%W=4.7%; %FO=11.3%; %N=11.1%). The diet did not vary between sexes. Ontogenic changes were detected: crustaceans decreased in importance as the sharks increased in size and fish became dominant in the diet of adults. Combining two fishing methods provided broad information on the diet of E. pusillus, as bottom trawling caught smaller specimens and longlines caught larger individuals. E. pusillus feeds mainly on non-commercial species, and therefore does not compete directly with commercial fisheries. Finally, E. pusillus feeds in various parts of the water column and thus it can access a wide range of prey; however, this also means that it can be caught by both gears, making it more vulnerable in terms of conservation.

Agronomic efficiency of Bradyrhizobium in peanut under different environments in Brazilian Northeast.

Several legumes have natural ability to associate with nitrogen - fixing bacteria known as rhizobia. The efficiency of this association depends on the plant and bacterial genotype and the edaphoclimatic conditions. Peanut is a tropical legume able to associate with a wide range of rhizobia and the selection of efficient bacteria is important to increase the nitrogen fixation in this crop. In order to investigate the agronomic efficiency of two Bradyrhizobiumstrains, two peanut genotypes were used in field trails carried out in three environments located at Brazilian Northeast. The genotypes (BR1 and L7 Bege) were submitted to rhizobial inoculation (SEMIA 6144 or ESA 123, both Bradyrhizobium strains, and chemical nitrogen fertilization in randomized block design experiments. The following traits were analyzed: flowering (F), main axis height (MAH), number of nodules/plant (NN), number of pods/plant (NP) and weight of pods (WP). Differential responses were found in all to treatments to NN, NP and WP, in the three environments studied. Overall, ESA 123 showed good agronomic performance inducing higher pod production. The results supportthe evaluation of the Bradyrhizobium in further experiments aiming at its recommendation to commercial inoculants in Brazilian Northeast region.

Sensory profiles of tropical wines produced in the São Francisco River Valley (Northeast Brazil).

2011

Determination of bioactive amines in tropical wines produced at Brazilian's Northeast.

The wine production in the region of the Sub-middle São Francisco river Valley has started there are few years ago.The region has a climate variability that allows to obtain two or three harvests per year, which influences significantly the composition of the grapes. This work aimed to evaluate the amount of amines present in white and red tropical wines produced in the Northeast of Brazil. The winemaking was done by the traditional method. After stabilizing the wines were bottled and then analyzed for the determination of the amines putrescine, spermidine and spermine, by thin layer chromatography (TLC). The harvests were in June (harvest I) and November (harvest II) 2009. The results showed that the edaphoclimatic conditions of the two harvests had significant influence (p> 0.05) on bioactive amines concentration, with high values for red wines. In addition, tropical wines from Sub-middle São Francisco river Valley showed adequate correlation between bioactive amines as compared to other wines in the world.