Método de utilização de membrana de celulose bacteriana intra articular sobre superfície lesada de patela

The present invention discloses the use of the bacterial cellulose membrane in ligament, tendon and synovial capsule reconstructions according to the methods described in the technical description of the invention. Said material could be used in the reconstruction of ligaments and tendons (the knee cruciate ligaments, patellar ligament, Achilles tendon, quadriceps tendon, etc.) and the synovial capsule.

Distribuição intra-planta de frutos atacados de goiaba por Conotrachelus psidii Marshall, 1922 (Coleoptera: Curculionidae) em cultivo orgânico

Pós-graduação em Agronomia (Entomologia Agrícola) - FCAV

Characterization of the intrahospital transport of critically ill patients

Objective: Characterizing the transport of critically ill patients in an adult intensive care unit.Methods: Cross-sectional study in which 459 intra -hospital transports of critically ill patients were included. Data were collected from clinical records of patients and from a form with the description of the materials and equipment necessary for the procedure, description of adverse events and of the transport team.Results: A total of 459 transports of 262 critically ill patients were carried out, with an average of 51 transports per month. Patients were on ventilatory support (41.3 %) and 34.5 % in use of vasoactive drugs. Adverse events occurred in 9.4% of transports and 77.3 % of the teams were composed of physicians, nurses and nurse technicians.Conclusion: The transport of critically ill patients occurred in the morning period for performing computerized tomographies (CT scans) with patients dependent on mechanical ventilation and vasoactive drugs. During the transports the equipment was functioning, and the adverse events were attributed to clinical changes of patients.

Intra-abdominal pressure as a predictor of acute kidney injury in postoperative abdominal surgery

Objectives: The purpose of this study was to determine if intra-abdominal pressure (IAP) could predict acute renal injury (AKI) in the postoperative period of abdominal surgeries, and which would be its cutoff value. Patients and methods: A prospective observational study was conducted in the period from January 2010 to March 2011 in the Intensive Care Units (ICUs) of the University Hospital of Botucatu Medical School, UNESP. Consecutive patients undergoing abdominal surgery were included in the study. Initial evaluation, at admission in ICU, was performed in order to obtain demographic, clinical surgical and therapeutic data. Evaluation of IAP was obtained by the intravesical method, four times per day, and renal function was evaluated during the patient's stay in the ICU until discharge, death or occurrence of AKI. Results: A total of 60 patients were evaluated, 16 patients developed intra-abdominal hypertension (IAH), 45 developed an abnormal IAP (>7 mmHg) and 26 developed AKI. The first IAP at the time of admission to the ICU was able to predict the occurrence of AKI (area under the receiver-operating characteristic curve was 0.669; p=0.029) with the best cutoff point (by Youden index method) >= 7.68 mmHg, sensitivity of 87%, specificity of 46% at this point. The serial assessment of this parameter did not added prognostic value to initial evaluation. Conclusion: IAH was frequent in patients undergoing abdominal surgeries during ICU stay, and it predicted the occurrence of AKI. Serial assessments of IAP did not provided better discriminatory power than initial evaluation.

Peptide diversity in the venom of the social wasp Polybia paulista (Hymenoptera): A comparison of the intra- and inter-colony compositions

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Estudo da distribuição das tensões internas, pelo método dos elementos finitos, em uma raiz com um retentor intra-radicular pré-fabricado, rosqueado e com fenda apical

Pós-graduação em Odontologia Restauradora - ICT

Avaliação in vitro da resistência estrutural em dentes fragilizados utilizando reforços intra-radiculares

Pós-graduação em Odontologia Restauradora - ICT

Inter- and intra-specific diversity of Cuban Pinguicula (Lentibulariaceae) based on morphometric analyses and its relation with geographical distribution

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Next-generation sequencing reveals large connected networks of intra-host HCV variants

Background: Next-generation sequencing (NGS) allows for sampling numerous viral variants from infected patients. This provides a novel opportunity to represent and study the mutational landscape of Hepatitis C Virus (HCV) within a single host.Results: Intra-host variants of the HCV E1/E2 region were extensively sampled from 58 chronically infected patients. After NGS error correction, the average number of reads and variants obtained from each sample were 3202 and 464, respectively. The distance between each pair of variants was calculated and networks were created for each patient, where each node is a variant and two nodes are connected by a link if the nucleotide distance between them is 1. The work focused on large components having > 5% of all reads, which in average account for 93.7% of all reads found in a patient. The distance between any two variants calculated over the component correlated strongly with nucleotide distances (r = 0.9499; p = 0.0001), a better correlation than the one obtained with Neighbour-Joining trees (r = 0.7624; p = 0.0001). In each patient, components were well separated, with the average distance between (6.53%) being 10 times greater than within each component (0.68%). The ratio of nonsynonymous to synonymous changes was calculated and some patients (6.9%) showed a mixture of networks under strong negative and positive selection. All components were robust to in silico stochastic sampling; even after randomly removing 85% of all reads, the largest connected component in the new subsample still involved 82.4% of remaining nodes. In vitro sampling showed that 93.02% of components present in the original sample were also found in experimental replicas, with 81.6% of reads found in both. When syringe-sharing transmission events were simulated, 91.2% of all simulated transmission events seeded all components present in the source.Conclusions: Most intra-host variants are organized into distinct single-mutation components that are: well separated from each other, represent genetic distances between viral variants, robust to sampling, reproducible and likely seeded during transmission events. Facilitated by NGS, large components offer a novel evolutionary framework for genetic analysis of intra-host viral populations and understanding transmission, immune escape and drug resistance.

Comportamento de ovipostura em Chrysomya megacephala (Fabricius) frente à presença de posturas prévias: competição intra e interespecífica

Em ambiente natural, as moscas-varejeiras depositam seus ovos geralmente em grupos de 100 a 300 ovos, freqüentemente na presença de ovos de outras espécies. Estes ovos são depositados em substratos discretos e efêmeros, que constituem unidades pequenas e separadas espacialmente, tais como carcaças, fezes, frutos, fungos e vegetais em decomposição, nas quais ocorrem mudanças sucessivas e rápidas. Em condições naturais, tais substratos apresentam-se saturados de indivíduos, de uma única ou mais espécies de insetos, principalmente em estágio larval, caracterizando uma intensa competição por recursos durante o desenvolvimento pós embrionário. Vários autores tentaram explicar a coexistência de moscas-varejeiras em substratos efêmeros, sugerindo que cada espécie pode ter seu próprio nicho dentro do substrato, uma especialização em diferentes partes do recurso alimentar ou comportamentos distintos de dispersão larval pós alimentar, que podem permitir a coexistência de espécies. No gênero Chrysomya, as similaridades nos nichos ecológicos, como por exemplo, no requerimento alimentar de suas larvas, podem acirrar a competição por recursos, e a espécie C. albiceps pode tornar-se predadora, apresentando comportamento de predação intra-guilda e canibalismo, interferindo na coexistência, no tamanho populacional das espécies presentes e podendo até excluir alguma delas. O nível de competição por espaço e alimento que os imaturos irão enfrentar relaciona-se com o padrão de distribuição espacial destes no substrato, decorrentes da escolha do sítio de oviposição pelas fêmeas adultas. Assim, o comportamento de oviposição é um processo biológico fundamental para as moscas, pois pode determinar a sobrevivência e o tamanho populacional nas sucessivas gerações. Por este motivo, o objetivo deste trabalho ...(Resumo completo, clicar acesso eletrônico abaixo)

Influência do uso de hipoclorito de sódio 2,5% e extrato glicólico de sálvia 20% durante o preparo biomecânico sobre a resistência adesiva de retentores intra- radiculares: avaliação através do teste de push-out

The aim of this study it was to evaluate the use of irrigating solution used during root canal preparation on the adhesive cementation of prefabricated fiberglass pins. The bond strength between different regions of the root and the fiberglass pins cemented into the root canal were evaluated by push-out assay. For this study, 36 human teeth were used, all equally prepared. The irrigating solutions used for biomechanical preparation were: saline solution, sodium hypochlorite 2,5% and glycolic extract of salvia 20%. The dual cure resin cement was used for cementation of pins. After cementation, specimens were sectioned to give 3 slices of approximately 2 mm each (cervical, middle and apical), which were submitted to mechanical push-out test. Were performed ANOVA and Tukey's test with a significance level of 5%, for the analysis of mechanical test. Comparing the thirds we observed that the only solution that presented statistical difference in the bond strength was sodium hypochlorite, which showed higher values for the middle and apical third compared with the cervical third, but when compared without taking into consideration the dental thirds, solutions studied showed no differences in bond strength. Therefore, it was concluded that the solutions studied can be used during endodontic treatment without causing harmful effects on the final adhesive restoration

Exposição perinatal ao chumbo e desnutrição intra-uterina, isoladamente ou em associação: efeitos cardiovasculares em ratos recém-desmamados

Plumbism is considered the oldest occupational disease. Among the pathophysiological effects associated with lead (Pb) are cardiovascular disorders. Many diseases that develop later in life are determined during the early stages of life, under the influence of exposure and preferred diet of the mother. Still, one should consider that many environmental contaminants at levels not harmful can determine pathophysiological processes if physical or chemical stressors and/or pathological conditions are present. In this context, the intrauterine malnutrition may represent an additional risk factor in exposure to Pb during pregnancy and lactation. For these reasons, the objective of this study was to evaluate the cardiovascular risk of weaned rats that have suffered perinatal exposure to Pb and intrauterine malnutrition, alone or in combination. After mating, female rats were divided into control (ctrl, ad libitum), food restriction (RA, the same diet 50% of consumption in the control group during pregnancy), exposed to Pb (500 ppm Pb in drinking water during pregnancy and lactation) and association (As, received the last two procedures in combination). Cumulative concentration-effect curves (CECs) to CaCl2 and noradrenaline (NA) were obtained in rings with and without endothelium of the same thoracic aorta from male weaned rats (23-25 days old). Maternal weight, litter weight, weight and number of pups at birth, anogenital distance, arterial blood pressure (ABP) and weight of tissues (kidney, liver, aorta, left ventricle) were evaluated. Changes in vascular reactivity were assessed by the maximum response (MR) and 50% effective concentration (EC50). Data were presented as mean ± SEM. Statistical analysis was performed by multifactorial analysis of variance and Tukey's post test. Body weight of dams did not differ between the experimental groups, except on the...(Complete abstract click electronic access below)

Intra-examiner reproducibility of the agar plate method for estmation of phospholipase activity in candida albicans

Although measurement errors can impair statistical analysis, reliability analysis has been neglected in applied microbiology. This study assessed the intra-rater reproducibility of the Agar-based method for estimation of phospholipase activity (Pz). Pz readings were performed twice by two examiners (E1, E2), either directly on plates or in photos, and both black and white backgrounds were used. Pz values were taken from one or triplicate colonies from each sample (n=30). Intra-examiner reproducibility was estimated using Intraclass Correlation Coefficient (ICC). For both examiners, reading triplicate (ICCE1=0.91, ICCE2=0.86) was better than one colony (ICCE1=0.86, ICCE2=0.80). E1 had an excellent concordance when measurements were performed on photos using a white background (ICC=0.95) and a good concordance in the other conditions

Fracture resistance of simulated immature teeth after different intra-radicular treatments

The aim of this study was to evaluate the fracture resistance of simulated immature teeth after different intra-radicular treatments. Crowns and roots of bovine incisors were cut transversally and removed to simulate immature teeth. Root canal preparation and flaring were performed using a bur in crown-apex and apex-crown direction. The samples were distributed into 5 groups (n=10): Positive control (PoC) - no root canal flaring or filling; Negative control (NeC) - teeth were sectioned and their root canals were flared; Direct anatomical glass fiber post (RaP) - #2 Reforpost main glass fiber post relined with composite resin; Double tapered conical glass fiber posts (ExP) - #3 Exacto glass fiber post; and #2 Reforpost main glass fiber + Reforpin accessory glass fiber posts (RrP). In RaP, ExP and RrP, 4.0-mm apical plugs were done with MTA Angelus. The specimens were embedded in polystyrene resin inside cylinders and the periodontal ligament was simulated with a polyether-based impression material. The specimens were submitted to compressive fracture strength test (0.5 mm/min at 135° relative to the long axis of the tooth) in a servo-hydraulic mechanical testing machine MTS 810. Data were subjected to one-way ANOVA and Dunnett's C or Tukey's tests (α=0.05). The control groups (PoC and NeC) showed lower fracture strength than the experimental groups. NeC presented the lowest resistance and ExP presented the highest resistance among the experimental groups. The flaring procedures produced a detrimental effect on the fracture resistance of the bovine teeth. Glass fiber intra-radicular posts increased significantly the fracture resistance of simulated immature teeth.

Estudo intra e interpopulacional de Pachycoris Torridus (Scopoli, 1772) (Heteroptera: Scutelleridae)

Pós-graduação em Genética - IBILCE