Influence of artificial aging in marginal adaptation of mixed class V cavities

The aim of this study was to investigate whether the artificial aging by thermal cycling had influenced the marginal adaptation of class V restorations with/without chlorhexidine application in the bond process. Twelve intact human third molars were used. Class V cavity preparations were performed on the buccal surface and the teeth received 35% phosphoric acid-etching procedure (Ultradent Products Inc., South Jordan, Utah, USA). Subsequently, the samples were divided in two groups: Untreated acid-etched dentin and chlorhexidine application as an adjunct in the bond process. The adhesive Single Bond 2 (3M ESPE, St. Paul, MN, USA) was used after 2% chlorhexidine application, and the restorations were performed with FiltekTM Z350 XT (3M ESPE) composite resin. The specimens were submitted to artificial aging by thermal cycling with 3,000 cycles. Analyzes were performed on scanning electron microscopy using replicas of marginal adaptation in percentage of continuous margin before and after the artificial aging. The data were analyzed by paired test and the results showed statistically significant differences in the percentage of continuous margin with/without chlorhexidine treatment before and after thermal cycling. This study concluded that the artificial aging by thermal cycling influenced the marginal adaptation of mixed class V composite restorations.

Structure-function relationships of the peptide Paulistine: A novel toxin from the venom of the social wasp Polybia paulista

Background: The peptide Paulistine was isolated from the venom of wasp Polybia paulista. This peptide exists under a natural equilibrium between the forms: oxidised - with an intra-molecular disulphide bridge; and reduced - in which the thiol groups of the cysteine residues do not form the disulphide bridge. The biological activities of both forms of the peptide are unknown up to now. Methods: Both forms of Paulistine were synthesised and the thiol groups of the reduced form were protected with the acetamidemethyl group [Acm-Paulistine] to prevent re-oxidation. The structure/activity relationships of the two forms were investigated, taking into account the importance of the disulphide bridge. Results: Paulistine has a more compact structure, while Acm-Paulistine has a more expanded conformation. Bioassays reported that Paulistine caused hyperalgesia by interacting with the receptors of lipid mediators involved in the cyclooxygenase type II pathway, while Acm-Paullistine also caused hyperalgesia, but mediated by receptors involved in the participation of prostanoids in the cyclooxygenase type II pathway. Conclusion: The acetamidemethylation of the thiol groups of cysteine residues caused small structural changes, which in turn may have affected some physicochemical properties of the Paulistine. Thus, the dissociation of the hyperalgesy from the edematogenic effect when the actions of Paulistine and Acm-Paulistine are compared to each other may be resulting from the influence of the introduction of Acm-group in the structure of Paulistine. General significance: The peptides Paulistine and Acm-Paulistine may be used as interesting tools to investigate the mechanisms of pain and inflammation in future studies. © 2013 Elsevier B.V.

O pensamento vivo de Mário de Andrade: dos parques infantis aos CEUs da cidade de São Paulo

Pós-graduação em Artes - IA

A modernidade poética em Cesário Verde e Gomes Leal

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

A comicidade nas crônicas de Mário de Andrade

Pós-graduação em Estudos Literários - FCLAR

Interação genótipo-ambiente em características de crescimento de bovinos da raça Nelore

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Estudo da eutrofização do reservatório da Floresta Estadual Edmundo Navarro de Andrade (FEENA) - Rio Claro (SP)

Pós-graduação em Geociências e Meio Ambiente - IGCE

Nacionalismo, cosmopolitismo e afrancesamento em Mon coeur balance e Leur âme, de Oswald de Andrade e Guilherme de Almeida

Pós-graduação em História - FCLAS

Utopia no front: história e filosofia em Oswald de Andrade 1945-1954

Pós-graduação em História - FCLAS

A França nas crônicas de Mário de Andrade: o nacional e o estrangeiro no periódico Diário Nacional (1929-1932)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

A fuga dupla luso-brasileira durante os séculos XVIII e XIX

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Saudade (1919-2002): a contribuição de Thales Castanho de Andrade para o campo da leitura escolar

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Propagação de Hancornia speciosa Gomes – Apocynaceae, por alporquia e micropropagação

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Mário de Andrade e a cultura popular brasileira

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Elementos-traço As, Ba, Cd, Cr, Cu, Hg, Mo, Ni, Pb, Se e Zn em latossolos e plantas de milho após treze aplicações anuais de lodo de esgoto

Pós-graduação em Agronomia (Ciência do Solo) - FCAV