Influence of nitrogen source and sucrose concentration on inulinase production by Kluyveromyces marxianus in fed-batch fermentation

This work studied the influence of nitrogen source and sucrose concentration in the feeding medium for biomass and inulinase production by Kluyveromyces marxianus var. bulgaricus. The results show that the best nitrogen source was a combination of 5 g/L of yeast extract and 10 g/L of peptone. Both cellular growth and enzymatic activity increased with sucrose concentration in the feeding medium (from 200 to 500 g/L). When the sucrose concentration reached 600 g/L, both cellular growth and enzymatic activity decreased.

Controle postural em pacientes com lesão do ligamento cruzado anterior

A lesão do ligamento cruzado anterior (LCA) do joelho acarreta alterações somatosensoriais em função da perda de informações provenientes dos mecanorreceptores presentes no LCA. Esses receptores constituem importante fonte de informação sensorial, afetando o desempenho de vários atos motores, dentre os quais o controle postural. O estudo objetivou analisar o controle postural de indivíduos com joelhos normais e com lesão unilateral do LCA. Participaram 15 voluntários com lesão do LCA (grupo lesado) e 15 voluntários com joelhos normais (grupo controle). O controle postural foi analisado por plataforma de força, sendo o voluntário instruído a assumir a situação experimental em apoio unipodal direito e esquerdo, posicionado no centro da plataforma de modo estático e com os olhos fechados. A plataforma de força forneceu informações de forças e momentos no eixo vertical e horizontal, a partir das quais foi obtida a área de deslocamento do centro de pressão nas direções ântero-posterior e médio-lateral. Os resultados mostram que indivíduos com lesão do LCA apresentaram maior amplitude média de oscilação comparados aos do grupo controle, sugerindo que o deficit no controle postural seja devido à perda de informações proprioceptivas nos indivíduos com LCA. Esses resultados têm implicações para a abordagem clínica de indivíduos com lesão do LCA.

The cetacean offal connection: Feces and vomits of spinner dolphins as a food source for reef fishes

At Fernando de Noronha Archipelago, southwest Atlantic, reef fishes associated with spinner dolphins (Stenella longirostris) were recorded when the cetaceans congregated in a shallow inlet. In the reef waters the dolphins engaged in several behaviors such as resting, aerial displays and other social interactions, as well as eliminative behaviors such as defecating and vomiting. Twelve fish species in seven families were recorded feeding on dolphin offal. The black durgon (Melichthys niger) was the most ubiquitous waste-eater, and its group size was positively and significantly correlated with dolphin group size. The durgons recognized the postures a dolphin adopts prior to defecating or vomiting, and began to converge to an individual shortly before it actually voided. Offal was quickly fed upon, and the fishes concentrated in the area occupied by the dolphins until the latter left the shallows. Since all the recorded offal-feeding species feed on plankton or drifting algae, feeding on cetacean droppings may be regarded as a switch from foraging on drifting organisms to foraging on drifting offal, a predictable food source in the inlet. Further instances of this cetacean-fish association are predicted to occur at sites where these mammals congregate over reefs with clear water and plankton-eating fishes.

On the use of open or closed traps in the capture of drosophilids

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Initiating structural studies of Lys49-PLA2 homologues complexed with an anionic detergent, a fatty acid and a natural lipid

Lys49-Phospholipase A(2) (Lys49-PLA(2) - EC 3.1.1.4) homologues damage membranes by a Ca2+-independent mechanism which does not involve catalytic activity. Both MjTX-II from Bothrops moojeni and BthTX-I from Bothrops jararacussu are dimeric in solution and in the crystalline states, and a model for the Ca2+-independent membrane damaging mechanism has been suggested in which flexibility at the dimer interface region pert-nits quaternary structural transitions between open and closed membrane bound dimer conformations which results in the perturbation of membrane phospholipids and disruption of the bilayer structure [1]. With the aim of gaining insights into the structural determinants involved in protein/lipid association, we report here the crystallization and preliminary X-ray analysis of the (i) MjTX-II/SDS complex at a resolution of 2.78Angstrom, (ii) MjTX-II/STE complex at a resolution of 1.8 Angstrom and (W) BthTX-I/DMPC complex at 2.72Angstrom. These complexes were crystallized by the hanging drop vapour-diffusion technique in (i) HEPES buffer (pH 7.5) 1.8M ammonium sulfate with 2% (w/v) polyethyleneglycol 400, in (ii) 0.6-0.8 M sodium citrate as the precipitant (pH 6.0-6.5) and in (iii) sodium citrate buffer (pH 5.8) and PEG 4000 and 20% isopropanol, respectively. Single crystals of these complexes have been obtained and X-ray diffraction data have been collected at room temperature using a R-AXIS IV imaging plate system and graphite monochromated Cu Kalpha X-ray radiation generated by a Rigaku RU300 rotating anode generator for (i) and (W) and using using a Synchrotron Radiation Source (Laboratorio Nacional de Luz Sincrotron, LNLS, Campinas, Brazil) for (ii).

Particular solution for anomalous diffusion equation with source term

In literature the phenomenon of diffusion has been widely studied, however for nonextensive systems which are governed by a nonlinear stochastic dynamic, there are a few soluble models. The purpose of this study is to present the solution of the nonlinear Fokker-Planck equation for a model of potential with barrier considering a term of absorption. Systems of this nature can be observed in various chemical or biological processes and their solution enriches the studies of existing nonextensive systems.

Closed string thermal torus from thermo field dynamics

In this Letter a topological interpretation for the string thermal vacuum in the thermo field dynamics (TFD) approach is given. As a consequence, the relationship between the imaginary time and TFD formalisms is achieved when both are used to study closed strings at finite temperature. The TFD approach starts by duplicating the system's degrees of freedom, defining an auxiliary (tilde) string. In order to lead the system to finite temperature a Bogoliubov transformation is implemented. We show that the effect of this transformation is to glue together the string and the tilde string to obtain a torus. The thermal vacuum appears as the boundary state for this identification. Also, from the thermal state condition, a Kubo-Martin-Schwinger condition for the torus topology is derived. © 2005 Elsevier B.V. All rights reserved.

Godel type solution in teleparallel gravity

The stationary cosmological model without closed timelike curves of Godel type is obtained for the ideal dust matter source within the framework of the teleparallel gravity. For a specific choice of the teleparallel gravity parameters, this solution reproduces the causality violating stationary Godel solution in general relativity, in accordance with the teleparallel equivalent of general relativity. The relation between the axial-vector torsion and the cosmic vorticity is clarified. (C) 2004 Elsevier B.V. All rights reserved.

Casimir energy density in closed hyperbolic universes

The original Casimir effect results from the difference in the vacuum energies of the electromagnetic field, between that in a region of space with boundary conditions and that in the same region without boundary conditions. In this paper we develop the theory of a similar situation, involving a scalar field in spacetimes with closed spatial sections of negative curvature.

Interaction of Hawking radiation with static sources in de Sitter and Schwarzschild-de Sitter spacetimes

We study and look for similarities between the response rates R-dS(a(0),Lambda) and R-SdS(a(0),Lambda,M) of a static scalar source with constant proper acceleration a(0) interacting with a massless, conformally coupled Klein-Gordon field (i) in de Sitter spacetime, in the Euclidean vacuum, which describes a thermal flux of radiation emanating from the de Sitter cosmological horizon and (ii) in Schwarzschild-de Sitter spacetime, in the Gibbons-Hawking vacuum, which describes thermal fluxes of radiation emanating from both the hole and the cosmological horizons, respectively, where Lambda is the cosmological constant and M is the black hole mass. After performing the field quantization in each of the above spacetimes, we obtain the response rates at the tree level in terms of an infinite sum of zero-energy field modes possessing all possible angular momentum quantum numbers. In the case of de Sitter spacetime, this formula is worked out and a closed, analytical form is obtained. In the case of Schwarzschild-de Sitter spacetime such a closed formula could not be obtained, and a numerical analysis is performed. We conclude, in particular, that R-dS(a(0),Lambda) and R-SdS(a(0),Lambda,M) do not coincide in general, but tend to each other when Lambda-->0 or a(0)-->infinity. Our results are also contrasted and shown to agree (in the proper limits) with related ones in the literature.

Scaler radiation emitted from a source rotating around a black hole

We analyse the scalar radiation emitted from a source rotating around a Schwarzschild black hole using the framework of quantum held theory at the tree level. We show that for relativistic circular orbits the emitted power is about 20-30% smaller than what would be obtained in Minkowski spacetime. We also show that most of the emitted energy escapes to infinity. Our formalism can readily be adapted to investigate similar processes.

On the birth of a closed hyperbolic universe

We clarify and develop the results of a previous paper on the birth of a closed universe of negative spatial curvature and multiply connected topology. In particular we discuss the initial instanton and the second topology change in more detail, This is followed by a short discussion of the results.