Fundamental studies in automatic vehicle control. Interim report.

Federal Highway Administration, Office of Research and Development, Washington, D.C.

Fundamental studies in automatic vehicle control. Final report.

Federal Highway Administration, Office of Research, Washington, D.C.

Factors contributing to the retention of seated passengers during emergency stops. Final report.

Urban Mass Transportation Administration, Washington, D.C.

Systems studies of automated highway systems: appendix I - conceptual automated highway system designs. Final report.

Federal Highway Administration, Traffic Systems Division, Washington, D.C.

Practicality of automated highway systems. Volume I - summary report. Final report.

Federal Highway Administration, Office of Research and Development, Washington, D.C.

Guidelines for desing and evaluation of human factors aspects of automated guideway transit systems. Final report.

Urban Mass Transportation Administration, Washington, D.C.

Systems studies of automated highway systems: appendix II - analysis of automated highway systems. Final report.

Federal Highway Administration, Traffic Systems Division, Washington, D.C.

Workshop on Methodology for Evaluating the Effectiveness of Transit Crime Reduction Measures in Automated Guideway Transit Systems. Proceedings. Final report.

Urban Mass Transportation Administration, Washington, D.C.

Where love and need are one : a demonstration of the use of subsidy to facilitate the adoption of black children in need of permanent homes /

Cover title: "Where love and need are one" : a report on the use of subsidies to increase adoption of black children.

Health planning methods : case studies from the 1978 International Workshop.

Pt. 2 sold only by National Technical Information Service.

Research relating to children.

Prepared pursuant to contract OCD-CB-02, Children's Bureau, Office of Child Development, U.S. Department of Health, Education, and Welfare.

Implications of prospective payment for the health care system and health planning /

"HRP-0906516."

Acute psychiatric bed need planning : issues and methodologies, methodological note no. 6 /

"HRP-0906627."

Guideline to reference gene selection for quantitative real-time PCR

Today, quantitative real-time PCR is the method of choice for rapid and reliable quantification of mRNA transcription. However, for an exact comparison of mRNA transcription in different samples or tissues it is crucial to choose the appropriate reference gene. Recently glyceraldehyde 3-phosphate dehydrogenase and P-actin have been used for that purpose. However, it has been reported that these genes as well as alternatives, like rRNA genes, are unsuitable references, because their transcription is significantly regulated in various experimental settings and variable in different tissues. Therefore, quantitative real-time PCR was used to determine the mRNA transcription profiles of 13 putative reference genes, comparing their transcription in 16 different tissues and in CCRF-HSB-2 cells stimulated with 12-O-tetradecanoylphorbol-13-acetate and ionomycin. Our results show that Classical reference genes are indeed unsuitable, whereas the RNA polymerase II gene was the gene with the most constant expression in different tissues and following stimulation in CCRF-HSB-2 cells. (C) 2003 Elsevier Inc. All rights reserved.

Inherited copper toxicosis with emphasis on copper toxicosis in Bedlington terriers

Canine copper toxicosis is an important inherited disease in Bedlington terriers, because of its high prevalence rate and similarity to human copper storage disease. It can lead to chronic liver disease and occasional haemolytic anaemia due to impaired copper excretion. The responsible gene for copper toxicosis in Bedlington terriers has been recently identified and was found not to be related to human Wilson's disease gene ATP7B. Although our understanding of copper metabolism in mammals has improved through genetic molecular technology, the diversity of gene mutation related to copper metabolism in animals will help identify the responsible genes for non-Wilsonian copper toxicoses in human. This review paper discusses our knowledge of normal copper metabolism and the pathogenesis, molecular genetics and current research into copper toxicosis in Bedlington terriers, other animals and humans. (C) 2004 Elsevier GmbH. All rights reserved.