Desenvolvimento da técnica de MALDI Imaging utilizando tecidos animais

Currently the study of important molecular compounds present in low abundance in some tissues has been a challenge for proteomic analysis classic. An analysis requires more exploratory investigation of small regions of a tissue or a group of cells. MALDI Imaging Technology (MSI) is an application of mass spectrometry facing the chemical analysis of intact tissues. Thus, advances in mass spectrometry MALDI being obtained by the integration of histology, the best methods and automation are the main tools of data analysis. This tool has become essential to analyze the spatial distribution of peptides and proteins throughout the tissue sections, providing an enormous amount of data with minimum sample preparation. Thus, the aim of this study was to develop the technique of MALDI Imaging using tissue from glioblastoma multiforme (GBM), a form of most common malignant tumor in the brain. For this we used the printer chemical ChIP-1000 (Chemical Inkjet Printer, Shimadzu) and mass spectrometer type Maldi-ToF-ToF (Axima Performance, Shimadzu), a search of the identifications were performed in databases such as SwissProt. We identified more than forty proteins with diverse functions such as proteins F-actin-capping and Thymosin to the structure and organization cellular and proteins such several Tumor necrosis factor receptor development-related pathology. The development of this technique will permit to carry-out proteomic analysis directly into the tissue, enabling earlier diagnosis of diseases, as well as the identification and characterization of potential biomarkers of disease.

Índigo assessoria: um processo de criação de marcas sob a ótica das relações públicas

Este estudo tem como objetivo demonstrar que as relações públicas podem ser um diferencial no processo de construção de marcas. A partir deste posicionamento apresentam-se as atribuições do relações-públicas nesse processo que se dá principalmente em proporcionar uma visão ampla sobre elementos como a cultura e a identidade organizacionais, além de conseguir estabelecer, com maior criticidade, relacionamentos com seus públicos de interesse, segundo suas prioridades. Partindo, de um levantamento teórico sobre tipologias organizacionais, a visão fundamentada do planejamento de uma marca, as atribuições do relações-públicas neste processo, até chegar à descrição da criação da marca Índigo Assessoria, o trabalho propõe que as fases de construção da marca devam ser embasadas em estudos sólidos de relações públicas para se obter sucesso e relevância no cenário global atual

Falência renal aguda em pequenos animais

The pets, mostly dogs and cats are susceptible to a variety of situations that can lead to acute kidney failure, such as infection and intoxication, besides other common causes such as dehydration or other pre-existing diseases. The abrupt decrease in renal function in these animals characterizes a severe clinical condition and requires specialized veterinary medical care since it increases the death. Early diagnosis is an important factor for the survival of these animals and so is the appropriate treatment. New technologies on diagnosis and treatment of serious diseases in veterinary medicine have made acute renal failure successfully reversed by hemodialysis in cases where conventional therapy may not be effective. In cases like that, hemodialysis treatment may be the only chance of survival for the animals, which makes this therapy more and more important and necessary, especially in referral centers of veterinary assistance

Caracterização da fauna recebida e avaliação dos procedimentos em Centros de Triagem de Animais Silvestres (CETAS)

Estima-se que anualmente, em função do tráfico de animais silvestres, cerca de 12 milhões de espécimes sejam retirados de nossas florestas, sendo que apenas uma ínfima porcentagem de indivíduos é recuperada e destinada à soltura com o devido rigor técnico. O tráfico de animais silvestres constitui um dos fatores mais relevantes da destruição da fauna, acarretando em um desequilíbrio na estruturação e manutenção dos ecossistemas. Este comércio ilegal constituise o terceiro maior do mundo, perdendo apenas para o tráfico de narcóticos e armas. Assim, para otimizar o processo de soltura da fauna como estratégia para conservação de espécies torna-se necessário uma análise quantitativa e qualitativa da fauna atendida e dos procedimentos realizados nos Centros de Triagem de Animais Silvestres (CETAS). Nesse sentido, o presente trabalho teve como objetivo fazer uma caracterização da fauna recebida e uma avaliação dos procedimentos realizados em dois CETAS, além de discutir sobre a importância desse órgão na triagem, reabilitação e conservação de animais silvestres. O presente estudo é justificado pela escassez de informações sobre a fauna retirada da natureza. Estas são necessárias para servir de base científica para a adoção de políticas públicas para o combate ao tráfico de animais silvestres; elaborar um programa de fauna que contemple a triagem e destinação adequada e contribuir para o desenvolvimento de trabalhos educativos com o fim de esclarecimento da sociedade. Foram analisados o “Livro de Registro” do CETAS da Fundação Animália e o “Livro de Plantão” do CETAS DEPAVE – 3, bem como os “Relatórios CETAS” que esses dois Centros enviaram ao IBAMA. O período escolhido foi do ano de 2003 a 2008. Os dados foram compilados e organizados em planilhas do Microsoft Office Excel e foram elaborados tabelas e gráficos para análise dos resultados.

Síndromes Paraneoplásicas em Pequenos Animais

Paraneoplastic syndromes are being increasingly recognized in dogs and cats in veterinary medicine as they often are oncological emergencies and may help in early diagnosis of primary neoplasm, performing a more effective treatment, which usually is the removal of the tumor, and thereby improving the quality of life of the animal and perhaps prognosis. The syndrome is caused by the tumor through the production of substances such as hormones and cytokines that are released into circulation and are responsible for the appearance of distant signals from the primary neoplasm. These syndromes can involve multiple systems of the body, among them we mention: the hematology, the dermatologist, the neurologist, endocrine and musculoskeletal

Aspectos gerais da síndrome anorexia caquexia do câncer em pequenos animais

The cancer anorexia-cachexia syndrome is the most common paraneoplastic syndrome in Veterinary Medicine. It is characterized by severe loss of muscle mass and adipose tissue resulting in severe unintentional weight loss, anemia, fatigue, negative nitrogen balance, immune dysfuntion and other metabolic disturbances. The SAC is not only a result of inadequate intake of nutrients. The tumor requires large amounts of nutrients to allow growth and causes changes in pacient metabolism to get this energy. Recent studies suggest that the metabolic changes by cancer can be measured by hormones and cytokines produced or by the patient or the tumor, but this not completely understood. Animals with SAC have lower survival time, the greater chance of complications during treatment and lower quality of life. With the increase in the number of cancer cases in domestic animals and longer lifespan after diagnosis of malignant disease through the use of antineoplastics drugs, diagnosis and treatment of cancer anorexia-cachexia syndrome has shown great importance in that patients may have higher survival then better quality of life. This paper aims to provide information about this complex and multifunctional syndrome and its possible treatments

Proposta de recuperação e manejo de nascente em área rural do município de Álvares Machado – SP

The lack of proper planning in intervention works of runoff water on rural properties and rural public roads leads to economic damage and loss of environmental quality. The impact of the action without technical base is soil erosion, siltation of water sources, loss of the quality and availability of water, invalidating areas for cultivation and livestock. The conservation of headwaters requires beyond regeneration of the environmental characteristics the repair and management of the degraded watercourse in order to control erosion. This graduate work in Environmental Engineering from Universidade Estadual Paulista Julio de Mesquita Filho - UNESP Presidente Prudente proposes a headwater recuperation and management in rural area of Álvares Machado municipality, São Paulo State, through the method of deep drainage applied to the case of a rural road near the outcrop of water. This technique consists in the installation of draining structures in sub-areas of the road surface, or sub-gutter and/or in the platform border in order to conduct the underground flow of water and reduce the loss of sediment in water flow. Therefore, was performed a historic study of use and occupation of land in the municipality of Álvares Machado, portraying transformations of the landscape caused by human action. Geology, geomorphology, pedology and hydrogeology data were collected from the study area in order to establish the conditions that led into the current situation of degradation and formulate an effective intervention strategy for civil works

Criação de novo procedimento de engenharia que possibilite a rastreabilidade das tubulações de uma indústria química

The number of piping in an industry is high. Through this piping are conducted several kind of products at several temperature and pressure conditions. In a chemical company, the piping quantity conducting harmful chemical products to human health and to the environment is higher. Nowadays the theme sustainability is often mentioned and harm to environment may cause irreversible damage to the human being, to the fauna, to the flora and to company´s credibility. In this context, controlling over the piping to avoid accidents is mandatory. The objective of this monograph is to create a procedure which enables the chemical companies piping traceability. This monograph analyses the several existent traceability system in the three economy sectors and approaches the technical question of industrial piping in order to create a procedure that achieves its objectives as a technical document and at the same time be economically feasible, with low complexibility level and high practicability. Some possibilities to elaborate this procedure had been studied, as the creation of an alphanumeric code and making with a chisel in the pipeline based on ASTM F2897 and the use of chips to store the information. However, the procedure which best meet the requirement as low cost and high applicability is filling out an electronic plan with information about welding process, welding certification, welding consumables and inspections

Bases para criação de um Centro de Voluntariado na UNESP - Guaratinguetá

Volunteering is seen as a form of citizen participation enabling to involve, mobilize, engage and articulate people and institutions around issues of collective interest. Both for the volunteer and for the community involved, voluntary action can promote learning, new experiences and interaction with people previously unknown, as well as create opportunities for professional and personal growth. (CENTRO DE VOLUNTARIADO DE SÃO PAULO, 2012). This work starts from a survey conducted in 2006 by Carvalho, who detected the interest of members of the campus community Guaratinguetá UNESP in performing volunteer work. Its objective is to continue the work mentioned and think of ways to support volunteering in the Guaratinguetá Engineering College, mainly through the creation of connections between stakeholders (individuals and institutions). For this, focused Volunteer Centers and their functional models. Such a center could be the ideal facilitator of relationships between volunteer candidates that integrate FEG / UNESP community and institutions interested in the collaboration of these people. The work highlighted the benefits that voluntary action can bring to those involved in it, and the role that a Volunteer Center at UNESP Guaratinguetá may play, in the promotion and expansion of social activities already undertaken. The study also indicates the need for a validation of the proposals presented here and later detailing way of acting of the Centre in its institutional placement and maintenance costs

Análise comparativa espanhol-português de expressões idiomáticas formadas a partir de nomes de animais

Este é um trabalho descritivo, que tem como objetivo principal comparar expressões idiomáticas do português brasileiro e do espanhol. O corpus utilizado para selecionar as expressões foi a coleção Prisma (A1-A2/B1-B2/C1-C2). Os idiomatismos selecionados foram os formados por nome de animais. Na primeira parte do trabalho, discorremos sobre Fraseologia, Expressões Idiomáticas e discutimos algumas diferenças entre estas e as colocações. Além disso, foram propostas algumas reflexões sobre a dificuldade do aprendizado das expressões por parte dos alunos. Selecionamos, no corpus acima citado, dezenove expressões idiomáticas e procedemos à descrição e análise de suas composições e de seus significados. A partir dessa análise, apresentamos uma comparação dessas expressões com seus equivalentes no português do Brasil. A partir dessa comparação, pudemos observar que alguns idiomatismos analisados, embora possuam equivalentes entre as duas línguas em questão, são formadas com nomes de animais diferentes. Isso se deve, sobretudo, à visão de mundo e à cultura de cada país. Há ainda expressões cujos equivalentes em português e em espanhol não se diferem por uma palavra, mas sim por várias, apesar do significado ser o mesmo. Observamos, ainda, a importância do aprendizado dos idiomatismos e o fato de nos níveis A1 e A2 da coleção analisada, não aparecerem expressões idiomáticas, comprovando que muitos consideram que é preciso ter um nível mais elevado na língua estrangeira para aprendê-las

Terapia transfusional em pequenos animais

Blood transfusion is a very important therapeutic and profilatic procedure. However, the most part of the transfusions in animals in Brazil are done with total blood. The transfusion therapy made in an appropriate way, with hemotherapic products, can save lives and warranties a better health to patients. In all transfusion procedures, the cross match test must be done because there are lots of different blood group types, based on erythrocytes antigen. A slow infusion rate and intensive patient monitoration help to prevent transfusion reactions, mainly hemolytic reactions

Desenvolvimento de uma plataforma analítica para aquisição de imagens moleculares em tecidos animais

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Elaboração de guia de campo para identificação de mamíferos por meio de suas pegadas

Desde a década de 1960, atividades humanas negativas vêm crescendo em relação ao meio ambiente. Diversos caminhos vêm sendo apontados como recursos para minimizar ou solucionar os problemas alarmantes da alteração ambiental. A educação ambiental é compreendida como um desses recursos e para sua efetivação são necessárias propostas que envolvem estratégias e recursos didáticos diferenciados . Este trabalho visou à criação de um guia destinado ao professor, para auxiliá-lo no desenvolvimento de aula de campo, com a temática mamíferos, favorecendo a conscientização dos alunos sobre a importância biológica desses animais e de sua conservação. O guia propõe uma atividade de campo para a aprendizagem dos alunos pela observação e identificação de pegadas de mamíferos presentes na Fazenda Edgárdia

Principais marcadores utilizados na avaliação da qualidade do sêmen de animais domésticos

Methods of semen cryopreservation allow changes in spermatic cells, such as damage in plasma and acrossomal membrane and modifications in mitochondrial function due to a disorder in the lipidic bilayer. For effective oocyte fertilization, spermatozoa require functional competent membranes, and intact organelles, acrosome and DNA. However, most laboratory methods used to evaluate semen quality are not highly correlated with fertilizing capacity. The discovery of a variety of fluorochromes and compounds conjugated to fluorescent probes has enabled an accurate assessment of the viability, integrity and function of spermatozoa. Among the most used probes that label the various compartments of the sperm cell there are the membrane impermeable fluorescent dyes to test the membrane integrity, as well as acylated dyes that pass the intact membrane. For the acrossomal integrity the most commonly used method is lectins labeled by a fluorescent probe. The acrosome reaction and spermatic capacitation is detected by the evaluation of membrane architecture and disorder of lipids in plasma membrane. Mitochondrial function can be determined using markers for their aerobic activity. The DNA status of spermatozoa has been determined using the metachromatic properties of Acridine Orange, and the DNA fragmentation can also be assessed by TUNEL assay. Finally, DNA condensation is analyzed using a single cell DNA gel electrophoresis assay that indicates DNA compactation. This monograph aims to compile the various tests used to detect damaged spermatozoa under cryopreservation methods, searching for improve the predictive value of semen analysis with the intention of a successful conception

Rodococose equina: uma revisão com ênfase na fisiopatogenia e tratamento

The rhodococcosis affects humans and animals. Equine are the most important species for rhodococcosis, which is considered the most debilitating disease in the creation of foals, leading to a mortality rates greater than 50% in this category. The disease is caused by Rhodococcus equi, a bacteria considered as a soil-born opportunistic microorganism, intracellular and ubiquitous. The disease in horses is manifested mainly in the form of pyogranulomatous pneumonia and less often in the form of enteric disorders and / or joint disorders. Transmission occurs mainly by ingestion of contaminated food and water, and inhalation in contaminated environment. The organism has mechanisms of evasion of the immune system, maintain viable in inside phagocytic cells, and induces piogranulomatous infections, leading to lesions of difficult treatment using conventional antimicrobials. Lipophilic drugs with good intracellular activity are required to successful treatment. The conventional treatment for foals is based on the combination of erythromycin and rifampin. However, there is a growing concern about the emergence of resistant strains, which makes increasingly studies on the development of alternative antimicrobials for therapy