Studies on the gonadosomatic index and fecundity of chapila (Gudusia chapra Ham.)

The study was conducted in pond to determine the fecundity and gonadosomatic index of chapila, Gudusia chapra. The male fish was found to attain sexual maturity at 7.7 cm and 7.41 g and that of the female at 9.3 cm and 14.65 g by standard-length and body-weight respectively. The investigated fishes were found to be male and female at the ratio of 1:3 and generally female was found to be larger than male. The fish was found to spawn for several months with two spawning peaks, one in April and another in August as indicated by the peaks of gonadosomatic index and ova diameter. Fecundity of the fish ranged from 25,220 to 154,528 with an average value of 72,383 and was found to increase with the increase in length and weight of the fish. The relationships between fecundity and standard-length, body-weight, gonad-length and gonad weight of the fish were found to be linear and significant.

Limnological conditions of naturally turbid ponds and its effects on fish growth

An experiment was conducted on six naturally turbid ponds in the village of Salakandi situated to the south-west corner of Bangladesh Agricultural University, Mymensingh for a period of three months from July to September '96. The experiment was performed by studying the physicochemical factors of water and soil, the biological factors such as densities of phytoplankton and zooplankton, and the growth of fishes. During the experimental period water turbidity varied among ponds. The highest value of turbidity was found to be 679±183.6 FTU in pond 1 and the lowest was 158±23.31 FTU in pond 4. The maximum and the minimum water transparency were recorded in the month of July and September respectably. The lowest net weight of fishes was found 28.8 kg/ha/year in pond 1 due to high turbidity and the highest was 35.8 kg/ha/year in pond 4 due to low turbidity. Most of the correlations between turbidity and transparency, phytoplankton, and zooplankton were significant at 1% and 5% levels.

Environmental parameters and incidence of white spot disease in Penaeus monodon (Fab.) farming

An investigation was carried out to monitor management practices and to find out whether there is any relationship with occurrence of deadly white spot disease and environmental parameters. Three semi-intensive and a improved traditional shrimp farms were selected in which mass mortality of shrimp (Penaeus monodon) by white spot disease occurred previously. The farms were situated at two different geographical locations. Two ponds from each farm at random were selected for the study. Out of eight investigated ponds, 6 ponds in three farms were affected by the disease during investigation period. The non-affected ponds had relatively lower stocking density, lightly different management practice and were located at different geographical area. There was no significant variation in water quality parameters among the affected and non-affected ponds. No significant variations were recorded in pond preparation, source of Post Larvae (PL), water and feed management among the affected and non-affected ponds. The observation indicated that pond micro-organisms in a farm may not the only cause of the disease but some external factors also might be responsible for the outbreak of this disease.

Study on the intestinal bacteria of Labeo rohita (Ham.)

The quantitative and qualitative aspects of intestinal bacteria of rohu fish (Labeo rohita) showed that total viable count of bacteria ranged from 9.9 x 106 to 1.4 x 107 CFU/g of intestine in different age groups of fish. The bacterial load was highest in the month of July and lowest in January. The genera of the isolates from intestine included Coryneform, Micrococcus, Flavobacterium, Cytophaga, Achromobacter, Aeromonas Enterobacteriaceae and Vibrio. Coryneform was the dominant group throughout the study period followed by Micrococcus and Enterobacteriaceae. Marked variations in the bacterial load and generic composition of intestinal bacteria were evident during the study period in different age groups of rohu fish.

Length-weight relationship and condition factor of black carp (Mylopharyngodon spiceus Richardson) in Bangladesh

The length-weight relationship and condition factor of Mylopharyngodon spiceus were determined. The result of the study showed the dependence of weight (W) on the total length (L) in the following form: W= 0.006L(super 3.156) or in the logarithmic form Log W=- 2.1851 + 3.156 Log L. Standard errors of length and weight were 0.674 cm and 3.214 g respectively. The co-efficient correlation "r" was found to be 0.972 which indicated that the relationship between length and body weight of the fish was highly significant. The t-test also indicated that the correlation between length and weight was significant. The range and mean value of condition factor (K) were 0.865 to 1.041 and 0.958 respectively.

Colossal loss of shell and fin-fish larvae during collection of Penaeus monodon (Fab.) fry in the rivers of Satkhira

This study was conducted in five river-estuaries of Satkhira from January to December '96. It was found that during the collection of each Peneaus monodon) post larva (PL), about 45 larvae of other shrimps, 12 individuals of fin-fishes and 530 macro-zooplankters were mercilessly destroyed. It was also recorded that about 11.6 million of P. monodon PLs were harvested annually from the study area. The sh1dy implies that colossal loss of shell and fin-fishes and other plankton resources is done by tiger shrimp fry collectors, and such massive destruction adversely affect the natural productivity and ecological balance of the coastal environment.

Predation by Channa striatus (Bloch) on Rana tigrina (Daudin), Puntius gonionotus (Bleekeer) and Labeo rohita (Hamilton) in the laboratory

The growth performance of a predatory snakehead, Channa striatus was tested by supplying tadpoles of Rana tigrina and fingerlings of Puntius gonionotus and Labeo rohita as prey for a period of 21 days in aquaria. Prey consumption by C. striatus was significantly different (P<0.05) for different prey used (T1 - R. tigrina, T2 - P. gonionotus, T3 - L. rohita). Tadpoles of R. tigrina were preferred by the predator (C. striatus) over P. gonionotus and L. rohita although tadpole is nutritionally inferior to each of P. gonionotus and L. rohita. Each predator rayed on 50-330 mg per day per g of their body weight. Fish preyed on tadpoles also showed the highest growth. Significant difference in weight gain was found between T1 and T2 and also between T1 and T3 but no difference was found between T2 and T3. Food conversion ratio (FCR) was found to be lowest in treatment T3 followed by the treatments T2 and T1 respectively.

Production of Thai sharpunti (Puntius gonionotus Bleeker) in polyculture with carps using low-cost feed

The effectiveness of duckweed and rice bran as a low cost supplementary feed was compared through a six months production trial of rajpunti (Barbodes gonionotus) with carps. Six earthen ponds of 360 m² each were used for the trial. Three ponds received duckweed, while the other three received rice bran as supplementary feed. Fish biomass after six months of rearing increased to an average of 2,056 kg/ha in ponds which received duckweed and 2,056 kg/ha in rice bran treated ponds. The net profit with duckweed and rice bran worked out to Tk. 69.752 and Tk. 73.480 kg/ha, respectively. This study revealed that duckweed is a low cost supplementary feed, particularly for farmers with limited income.

Minimization of cannibalism of African catfish (Clarias gariepinus Burchell) larvae in indoor culture system

For minimizing cannibalism of African catfish (Clarias gariepinus) larvae two trials for a period of 14 and 15 days respectively in four aquaria of size 120x49x32 cm³ were conducted. Seven days old African catfish larvae with an initial total length and weight of 7.84 (±0.40) mm and 4.40 (±1.18) mg respectively in the first trial and similarly 7.52(±0.61) mm and 3.98 (±0.56) mg in the second trial at the rate of same stocking densities of 2500 larvae in each aquarium were stocked in both trials. Cannibalistic larvae were separated by using grader frame from each treatment at 7 days and 5 days interval during first and second trial respectively. Two mesh sizes i.e., 5 mm and 7 mm were used in the grader frame in both trials. Survival rate was significantly higher in T1 than that of T2 in each trial. Grading of larvae with 5 days interval resulted higher survival rate than that of 7 days interval.

Pathogenicity of Aeromonas hydrophila artificially infected to Indian major carp (Cirrhinus mrigala Ham.)

Abrasion, feeding, injection and immersion methods were used to evaluate the pathogenicity of five different strains of Aeromonas hydrophila viz. RG (rui gill), ML (mrigal lesion), SG (sharpunti gill), F1K (mrigal kidney), GFL (gold fish lesion) and Ah-19 (Aeromonas hydrophila-19, Ref. Strain) against C. mrigala H. Bacterial suspension containing viable cells of 7.5x 10⁵ per ml was found to be very effective in intramuscular injection and feeding resulting 100% mortality after 96hr of inoculation. The strain RG, ML and F1K produced scale loss with erosion of the skin surface with/without hemorrhagic lesion after 48hr of inoculation following abrasion method. The strains SG and Ah-19 resulting scale loss with erosion of the skin surface with/without hemorrhagic lesion after 72hr of inoculation following abrasion and injection methods. SG and F1K caused reddening in mouth region after 72hr of feeding inoculation, whereas RG resulted frank ulcers from eroded dermal layer exposing underlying musculature which was hemorrhagic after 96hr of inoculation by abrasion method.

Larval development of a mangrove crab (Perisesarma bidens De Haan) (Crustacea: Brachyura: Sesarminae)

Larval development of Perisesarma bidens (De Haan) was investigated in laboratory conditions. Morphology of all larval stages and 1st crab stage was described and illustrated in detail, and compared with other species of sesarmid crabs. The zoeae morphological features of P. bidens are almost similar to other species of Sesarma in lacking a pair of lateral spines on carapace.

Culture of pearl in freshwater mussels (Lamellidens marginalis Lamarck)

A pond trail on pearl culture in freshwater mussels, Lamellidens marginalis was carried out for one year in an artificial perennial pond. Four types of foreign particles of indigenous sources, sand, stone, fish eyeball and beads of artificial pearl nucleus were used as nucleus for pearl production. Among the nuclei inserted mussel highest survival rate (72%) was recorded for stone and lowest survival rate (50%) for artificial pearl by nucleus implantation. Highest pearl production rate (%) was recorded for the insertion of stone and lowest for the sand. All nuclei inserted mussel produced pearl accept the mussel which was inserted beads of pearl nucleus for pearl formation. Growth rate (length and weight) was found higher for uninserted mussel than nuclei inserted mussels.

Water quality management on the enhancement of shrimp (Penaeus monodon Fab.) production in the traditional and improved-traditional ghers of Bangladesh

On-farm research on enhancement of P. monodon production through water quality management was carried out in five ghers of Paikgacha, Khulna. Based on the prevailing condition of the ghers, lime in the form of CaCO(sub 3), urea and TSP were used as the major inputs to minimize the soil-water acidity and to ensure the availability of natural food particles in the water bodies. Exchange of water at required level also practiced for the qualitative improvement of culture water. Ghers of varying sizes showed that water quality management and fertilization have a positive impact on production performance of P. monodon (61.59% increment) that yielded an average production of 385.43 kg/ha/crop against the present traditional rate of 238.50 kg/ha/year.

Studies on the post-mortem changes in shrimp and prawn during ice storage. Pt. 1. Organoleptic and physical changes

The organoleptic characteristics such as appearance, textural condition, colour and odour indicated that the M. rosenbergii stored in ice for 5-6 days was acceptable for processing in the industry while P. monodon under similar ice storage condition was acceptable for 8-9 days. In both species, samples stored in headless condition in ice had longer shelf life than that of stored in head-on condition. Physical changes were evaluated by determining expressible moisture and breaking strength of sample of muscles. The expressible moisture increased continuously in both samples with the lapse of storage period. The expressible moisture increased up to around 44% in 4-5 days of ice stored M. rosenbergii muscle while it was around 40% in 8-9 days ice stored P. monodon. At the end of 9 days of ice storage, the expressible moisture content in M. rosenbergii increased up to 60%, while it was up to 47% in P. monodon after 11 days of ice storage. The breaking strength declined from 0. 78 kg/cm² to 0.53 kg/cm² in tiger shrimp after 8 days of ice storage, while in case of immediately killed prawn, the breaking strength of muscle was 0.8 kg/cm² which declined to 0.43 to 0.35 kg/cm².

Studies on the post-mortem changes in shrimp and prawn during ice storage. Pt. 2. Biochemical aspects of quality changes

Studies were conducted on biochemical changes in P. monodon and M. rosenbergii during ice storage. At the end of 10 days of ice storage, moisture and protein content of freshwater prawn slightly decreased from 78.34 to '77.35% and 18.46 to 17.10, respectively, while lipid and ash content slightly increased. The moisture, crude protein, lipid and ash content of one day ice stored tiger shrimp samples were 78.07, 18.06, 1.3 and 1.29% respectively. The protein composition of freshwater prawn immediately after killed were 36.51% sarcoplasmic, 44.63% myofibrillar, 8.12% stroma and 6.44% alkali soluble protein. At the end of 10 days of ice storage, sarcoplasmic and stroma protein slightly decreased while there was little or no changes observed in myofibrillar and alkali soluble protein. In case of one day ice stored tiger shrimp, the composition of protein were 35.32% sarcoplasmic, 46.29% myofibrillar, 7.86% stroma protein and 7.08% alkali soluble protein. At the end of 10 days in ice, sarcoplasmic protein decreased from 35.32% to 32.16% while there was slight change in other protein fractions. The TVB-N value of 1 day ice stored shrimp was 10.5 mg/100g of sample. It increased gradually with the lapse of storage period and at the end of 10 days storage in ice, the value increased up to 60 mg/100g sample. The tiger head on shrimp in ice storage were found organoleptic acceptable condition for 8 days and at that time the TVB-N values were 32.2 mg/100g which is slightly above the recommended limit for TVB-N for export.