937 resultados para banana cultivars


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本研究通过对玉簪属植物的组织培养,发现以腋芽、花序、试管苗的叶片为外植体,均可达到迅速繁殖的目的,但对于不同的玉簪品种来说,所能采用的外植体的种类又不尽相同:对于非嵌合体玉簪品种,以腋芽、花序、试管苗的叶片作外植体都可以;而对于嵌合体玉簪品种,如果以花序或试管苗的叶片为外植体,通过诱导不定芽进行快繁,在所得的试管苗中,超过50%的个体失去斑叶特性,这在实际生产中是不可行的,如果以腋芽为外植体,所得试管苗中多于95%的可保持原有嵌合体特征,故为理想的外植体。 在以腋芽为外植体的离体培养中,取嵌合玉簪品种H. ‘Francee’和H. ‘Ground Master’的芽作材料,通过对试验结果的分析、比较,选出了合适的繁殖培养基:MS + BA0.5mg/L + NAA0.5mg/L + KH2PO4150mg/L + 水解乳蛋白500mg/L + 蔗糖30g/L + 琼脂 5.6g/L,其可提高腋芽分化率,达到了快速繁殖的目的,此外,该培养基还可保持原来的生长势,降低斑叶特性分离比;同时选出了生根培养基:MS + IAA1mg/L + 蔗糖30g/L + 琼脂5.6g/L。 以花葶为外植体的试验,证实了此种方式仅适合非嵌合体玉簪的快速繁殖。 在以叶片为外植体的研究中,取了嵌合体品种H. ‘Francee’、H. ‘Ground Master’、H. ‘Gold Standard’、H. ‘Color Glory’和H. ‘Little Ming’的再生叶征作材料,通过对试验结果的分析、比较、观察到在培养基:MS + BA4mg/L + NAA0.1mg/L + 蔗糖 30g/L + 琼脂 5.6g/L上只有芽的形成,培养基:MS + BA0.4mg/L + NAA0.4mg/L + 蔗糖 30g/L + 琼脂 5.6g/L则利于根的发生,而培养基:MS + BA4mg/L + NAA0.4mg/L + 蔗糖 30g/L + 琼脂 5.6g/L促进根或(和)不定芽的产生,则因品种的不同而变化,另外还发现,经诱导不定芽途径所得到的试管苗,仅少于5%的个体保持斑叶特性,所以不可用于嵌合体玉簪的组织培养,但可快速繁殖非嵌合体玉簪。

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Two BFRI evolved aquaculture technologies - integrated rice fish farming and carp polyculture with over-wintered fingerlings under different stocking densities were tested during 2003-04. The study was coordinated with two local NGOs namely NICHAITA and JNDP, Muktagacha, Mymensingh. Integrated rice fish farming technology was demonstrated in 9 plots each having an area between 60-100 dec. during boro season. Fifteen days after transplantation of rice seedlings, fingerlings of rajpunti (Barbodes gonionotus) of 7-10 g of individual weight were stocked in the rice fields at the density of 3,000 (T1), 3,750 (T2) and 4,500/ha (T3). The corresponding final weight of fish after three and half months in treatments 1, 2 and 3 were 110±14.21, 101±16.55 and 86±22.28 g, respectively. The mean weight of fish in treatments 1 and 2 was significantly higher than treatment 3. Fish production obtained from treatments 1, 2 and 3 were 218.16±18.29, 239.70±25.11 and 236±24.66 kg/ha, respectively. On-farm demonstrations of carp polyculture using over-wintered fingerlings rohu (25-28 g), catla (24-26 g), mrigal (21-26 g) and grass carp (20-24 g) under different stocking densities were undertaken in nine earthen ponds (1,200-1,600 square meters) for a period of six months at three different stocking densities. The stocking densities of treatment 1 (T1), treatment 2 (T2) and treatment 3 (T3) were 2,000, 3,000 and 4,000/ha, respectively. Fish were fed with rice bran and mustard oil cake (3:1). Soft green grass and banana leaves were provided mainly for grass carp. At harvest, the production obtained in treatments 1, 2 and 3 were 2,325±74.75, 2,620±49.66 and 2,982± 171.52 kg/ha, respectively. The results demonstrated higher growth of fish in treatment 1 than those of treatments 2 and 3. However, treatment 3 contributed relatively higher production than those of treatments 1 and 2, whereas, highest net benefit was received from treatment 2.

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Background: Various evolutionary models have been proposed to interpret the fate of paralogous duplicates, which provides substrates on which evolution selection could act. In particular, domestication, as a special selection, has played important role in crop cultivation with divergence of many genes controlling important agronomic traits. Recent studies have indicated that a pair of duplicate genes was often sub-functionalized from their ancestral functions held by the parental genes. We previously demonstrated that the rice cell-wall invertase (CWI) gene GIF1 that plays an important role in the grain-filling process was most likely subjected to domestication selection in the promoter region. Here, we report that GIF1 and another CWI gene OsCIN1 constitute a pair of duplicate genes with differentiated expression and function through independent selection. Results: Through synteny analysis, we show that GIF1 and another cell-wall invertase gene OsCIN1 were paralogues derived from a segmental duplication originated during genome duplication of grasses. Results based on analyses of population genetics and gene phylogenetic tree of 25 cultivars and 25 wild rice sequences demonstrated that OsCIN1 was also artificially selected during rice domestication with a fixed mutation in the coding region, in contrast to GIF1 that was selected in the promoter region. GIF1 and OsCIN1 have evolved into different expression patterns and probable different kinetics parameters of enzymatic activity with the latter displaying less enzymatic activity. Overexpression of GIF1 and OsCIN1 also resulted in different phenotypes, suggesting that OsCIN1 might regulate other unrecognized biological process. Conclusion: How gene duplication and divergence contribute to genetic novelty and morphological adaptation has been an interesting issue to geneticists and biologists. Our discovery that the duplicated pair of GIF1 and OsCIN1 has experiencedsub-functionalization implies that selection could act independently on each duplicate towards different functional specificity, which provides a vivid example for evolution of genetic novelties in a model crop. Our results also further support the established hypothesis that gene duplication with sub-functionalization could be one solution for genetic adaptive conflict.

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The purpose for which this study was intended wasto compare nutritive value among the farmed Vannamei, sea Green Tiger and Banana shrimps native to the PersianGulf. To provide the samples of farmed shrimps at the end of the farming season (Oct. 23rd through Nov. 22nd of 2011), we chosen one farm of the Holleh Shrimp Farming, from which 100 shrimps were randomly selected. From among these 100 shrimps, 3 to 5 ones were taken to conduct an analysis upon. Further, to obtain the Banana and Green Tiger shrimps sampling was done at the fishing season (July 23rd through Aug. 22rd of 2011) at Halileh Fishing Wharf located in Bushehr Fishing Harbor and also Bandar Abbas Wharf. The samples obtained were immediately kept in the ice powder. After some biometric tasks done upon them, they were at the shortest possible time transferred to a laboratory where they went through various experiments to determine their content of raw protein, fat, ash, moisture, various fatty acids and their types, cholesterol, vitamins A and E, and such mineral elements as iron and calcium. All the experimentswere carried out three times to establish confidence in the results to be obtained. Findings of the comparison showed the content of raw protein, fat, moisture, and ash of, respectively, 23.233%, 600%, 73.077% and 2.500% for the Vannamei samples, of 22.717%, 427%, 74.133% and 1.826% for our Banana shrimps and of 17.377%, 430%, 79.866% and 1.313% for the Green Tiger samples. A total of 24 fatty acids for the Vannamei shrimps and 27 for the Banana and Green Tiger were detected. SFA of the Banana shrimps was 368.45 mg/100g (51.76%), while those of the Vannamei and Green Tiger samples were observed, respectively, 363.54 mg/100g (37.26%) and 296.06 mg/100g (49.12%).A similar measurement for MUFA content of the three types of our samples revealed 243.85mg/100g (24.9%) for the Vannamei, 203.177 mg/100g (33.76%) for the Green Tiger and 179.033 mg/100g (25.14%) for the Banana shrimps. The content of PUFA unsaturated fatty acids in the Vannamei, 131 Green Tiger and Banana samples were, respectively, 370.660 mg/100g (37.84%), 101.573 mg/100g (16.9%) and 163.733 mg/100g (23.1%). Further, the comparison found a omega-3-fatty-acids total of 151.747 mg/100g(15.51%) for the Vannamei, 57.123 mg/100g (9.54%) for the Green Tiger and 130.460 mg/100g (18.46%) for the Banana species under study.

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Goal, Scope and Background. As one of the consequences of heavy metal pollution in soil, water and air, plants are contaminated by heavy metals in some parts of China. To understand the effects of heavy metals upon plants and the resistance mechanisms, would make it possible to use plants for cleaning and remediating heavy metal-polluted sites. Methods. The research results on the effects of heavy metals on plants and resistant mechanisms are compiled from Chinese publications from scientific journals and university journals, mostly published during the last decade. Results and Discussion. Effects of heavy metals on plants result in growth inhibition, structure damage, a decline of physiological and biochemical activities as well as of the function of plants. The effects and bioavailability of heavy metals depend on many factors, such as environmental conditions, pH, species of element, organic substances of the media and fertilization, plant species. But, there are also studies on plant resistance mechanisms to protect plants against the toxic effects of heavy metals such as combining heavy metals by proteins and expressing of detoxifying enzyme and nucleic acid, these mechanisms are integrated to protect the plants against injury by heavy metals. Conclusions. There are two aspects on the interaction of plants and heavy metals. On one hand, heavy metals show negative effects on plants. On the other hand, plants have their own resistance mechanisms against toxic effects and for detoxifying heavy metal pollution. Recommendations and Outlook. To study the effects of heavy metals on plants and mechanisms of resistance, one must select crop cultivars and/or plants for removing heavy metals from soil and water. More highly resistant plants can be selected especially for a remediation of the pollution site. The molecular mechanisms of resistance of plants to heavy metals should be studied further to develop the actual resistance of these plants to heavy metals. Understanding the bioavailability of heavy metals is advantageous for plant cultivation and phytoremediation. Decrease in the bioavailability to farmlands would reduce the accumulation of heavy metals in food. Alternatively, one could increase the bioavailability of plants to extract more heavy metals.

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Primula obconica was introduced to Europe from Hubei, China in 1880, and has been cultivated worldwide as one of popular ornamental plants. The volatile oil of wild P. obconica collected from its original place, Yichang, Hubei was first investigated. A total of 43 compounds constituting 93.49% of the oil were identified by using GC and GC-MS. The major compounds were methyl 2,4-dihydroxy-5-methyl benzoate (30.41%), methyl 2,6-dihydroxy-4-methyl benzoate (29.27%), and hypnone (8.92%) etc. In comparison with the published data of some European cultivars, the native P. obconica seems to be allergen-free due to absence of primin and miconidin.

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Removal efficiencies on xenobiotics from polluted water in a twin-shaped constructed wetland consisting of a vertical flow chamber with the crop plant Colocasia esculenta L. Schott and a reverse vertical flow one with Ischaemum aristatum var. glaucum Honda, were assessed by chemical analysis and bioassays. After a four-month period of application, removal efficiencies of the applied pesticides parathion and omethoate were 100%, with no detectable parathion and omethoate in the effluent. For the applied herbicides, the decontamination was less efficient with removal efficiencies of 36% and 0% for 4-chloro-2-methyl-phenoxyacetic acid and dicamba, respectively. As shown by toxicity assay with duckweed Lemna minor L., growth retardation may occur if the water treated for herbicide removal is used in irrigation of sensitive cultivars in agriculture or horticulture. In contrast to I. aristatum var. glaucum Honda, the crop C esculenta L. Schott has a high yield in biomass production as a valuable source of renewable energy. (C) 2002 Elsevier Science Ltd. All rights reserved.

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采用2种不同夏玉米基因型(陕单9号,抗旱品种;陕单911,不抗旱品种)的盆栽试验,研究了长期水分胁迫下氮、钾对各生育期叶片净光合速率、蒸腾速率、胞间二氧化碳浓度和叶绿素含量的影响,旨在从光合生理特性揭示这些因子的抗旱机理。结果表明,长期水分胁迫下叶片净光合速率,蒸腾速率、胞间二氧化碳浓度(除成熟期)和叶绿素含量显著降低,不抗旱品种降幅更甚。抗旱品种的净光合速率和叶绿素含量大于不抗旱品种,而蒸腾速率和胞间二氧化碳浓度则相反。两品种苗期光合作用较弱,净光合速率和叶绿素含量均较低,抽雄期达到高峰。施氮能不同程度降低水分胁迫下玉米叶片的蒸腾速率,增加叶绿素含量.提高净光合速率,从而减缓水分胁迫对光合作用的伤害。随氮肥用量增加,不抗旱品种净光合速率和叶绿素含量显著升高,蒸腾速率和胞间二氧化碳浓度明显降低,两种氮肥用量间有显著差异;抗旱品种在低氮用量时效果显著,但高低氮用量间无显著区别。钾对受水分胁迫的玉米表现出比氮肥更突出的效果。相反,在适量供水条件下,氮、钾肥的作用明显下降。以上结果表明,适当用量的氮、钾肥可以有效地改善水分胁迫下作物叶片的光合特性,从而增强作物的抗旱性。

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盆栽试验方法研究充分供水(W)、适度干旱(M)和严重干旱(D)三种条件下,不同株型的两个大豆品种晋大74和晋豆24在混播和单播时的籽粒产量及生物量构成。结果表明:晋大74品种具有较高的植株生物量和较强竞争能力,在单播时籽粒产量较低,而竞争能力较低的晋豆24品种在单播时籽粒产量却较高,这一结论将对大豆株型设计提供一定的科学依据。

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探讨盐胁迫对苜蓿幼苗不同器官生长及抗氧化酶系统的影响,为苜蓿植被恢复技术的研发提供理论参考。【方法】以新牧一号(盐忍耐品种)和北极星(盐敏感品种)2个苜蓿品种为材料,以0mmol/L NaCl处理为对照,用200mmol/L NaCl胁迫处理,测定萌发7d苜蓿幼苗芽、根生长及其H2O2、丙二醛(MDA)含量,相对质膜透性、抗氧化保护酶活性及其同功酶的变化。【结果】200mmol/L NaCl胁迫抑制了苜蓿幼苗的生长,并导致芽、根H2O2、MDA含量及相对质膜透性升高,但新牧一号芽、根器官均表现出比北极星较低程度的生长抑制或质膜损伤。盐胁迫后超氧化物歧化酶(SOD)、抗坏血酸过氧化物酶(APX)、过氧化氢酶(CAT)、过氧化物酶(POD)的活性均明显增加。CAT和POD对活性氧的清除具有器官差异性,CAT活性在芽中较强,POD则与之相反。SOD-Ⅱ、APX-Ⅰ、POD-Ⅲ及POD-Ⅳ为芽、根中盐胁迫敏感的同功酶谱带。【结论】苜蓿盐忍耐品种通过更强的抗氧化保护能力,降低了盐胁迫对其幼苗的损伤。

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研究干旱对小麦旗叶光合产物供应能力的影响,揭示小麦抗旱高产的生理机制,为提高小麦的抗旱能力及高产稳产提供理论依据。【方法】在防雨池栽培条件下,以旱地冬小麦品种长武134(抗旱性强)和水地冬小麦品种陕253(抗旱性弱)为试材,以适宜水分处理为对照(CK,土壤含水量为田间持水量的70%~75%),研究干旱处理(土壤含水量为田间持水量的50%~55%)对不同冬小麦旗叶光合产物供应速率(净光合速率和蔗糖合成能力)和供应持续期的影响。【结果】与对照相比,干旱处理降低了冬小麦灌浆中后期旗叶净光合速率,缩短了净光合速率高值持续期(PAD),其中长武134降幅较小,净光合速率较高;干旱处理提高了冬小麦灌浆初期旗叶的蔗糖磷酸合成酶(SPS)活性,其中长武134增幅较大,且在灌浆中后期依然能保持相对较高的蔗糖供应能力;干旱处理缩短了冬小麦叶绿素含量缓降期(RSP),提高了丙二醛(MDA)含量,加速了旗叶的衰老,缩短了光合产物的供应持续期,其中长武134受干旱影响较小;干旱处理降低了冬小麦灌浆中后期主茎穗粒质量积累量及其速率,其中长武134降幅较小。【结论】干旱条件下,抗旱品种长武134旗叶在灌浆中后期可维持较高的光合产物...

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为了解黄土高原沟壑区小麦品种演替过程中的籽粒灌浆特性,以此地区20世纪50年代至今的主栽小麦品种为供试材料,研究了不同小麦品种演替过程中籽粒干物质积累量及灌浆速率的变化。结果表明,黄土高原沟壑区小麦演替过程中,小麦籽粒千粒重呈现逐渐增加的趋势。平均灌浆速率逐渐增加,小麦籽粒的干物质积累随之增加,长旱58的W0最大,达50.53 g。灌浆三阶段中,各阶段的灌浆速率表现为,V2>V1>V3。从灌浆持续时间看,T1和T2持续的时间较长且变异系数较大,而T3和T相对稳定。从灌浆速率来看,V1、V2、V3、Vm、Va变异系数较大,灌浆速率易受环境因素影响而波动。

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非手性的香蕉型分子因为独特的形状和极性自组装性能,能形成具有手性结构和特殊光电性能(如铁电性、反铁电性,二次谐波产生效应)的液晶,是近年来有机光电领域中一类非常令人感兴趣的材料。本论文以三环弯核分子间一二(对辛烷氧基苯乙烯基)苯[m-bis(4-p-octoxysryrenyl)benzene,m-OSB]为模型化合物,探索香蕉型分子真空气相沉积薄膜的生长行为。首先,采用示差扫描量热法(DSC)、偏光显微镜、X一射线衍射、透射电子显微镜以及分子模拟等手段表征了m一OSB的体相结构。m-OSB存在两个晶相:相I(T<66℃)和相11(66oC<T<157℃),157oC以上为各向同性态。确定相I为正交晶系,PZI2121空间群,晶胞参数为a=7.43A,b:6.34A,c=72.07A,α=β=γ=90°;分子模拟结果显示m一OSB排列成反铁电的层状结构,分子的长轴方向与c轴平行。相n的结构与相I非常相似。这些表征结果为薄膜形态结构的解释提供了依据。然后,采用真空蒸镀的方法制备m-OSB的单层和多层薄膜,用原子力显微镜、透射电子显微镜以及X-射线薄膜反射等手段研究了薄膜形态、结构和薄膜对温度的稳定性。由于香蕉型分子的特殊形状,m-OSB的气相沉积薄膜表现出了特殊的形貌、生长机理和相行为。在单层薄膜生长中,二维岛在40℃以下为圆形或椭圆形的滴状岛,在60℃以上为枝晶状岛,该生长形貌随基底温度的演变与传统的扩散受限凝聚(DLA)理论所预言的结果相反,AFM观察发现滴状岛是从最初沉积的无序膜分解得来的,类似"去润湿"现象;枝晶状岛是从无序膜中成核再通过分子的扩散生长形成的;单层薄膜无论是滴状岛还是枝晶状岛都不稳定,在室温下放置一段时间后均转化为双层的晶体;在多层膜的生长中,观察到了m-OSB形成了高有序、大尺寸的准外延膜。这些在有机气相沉积薄膜生长中都是第一次报道,论文对所观察到的生长现象和相变现象给予了合理的解释。通过对m-OSB气相沉积薄膜的成核和生长过程的系统研究,获得了薄膜形态结构与分子形状和制备参数的关系,对进一步理解有机分子气相沉积薄膜的生长规律和制备高有序的连续薄膜具有重要意义。

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随着工农业的快速发展,土壤重金属污染问题日益严重,在我国东北,大面积的蔬菜种植地受到Cd、Pb的污染。大白菜在东北地区一直得到广泛种植,然而,Cd、Pb单一及复合污染对这一地区大白菜的胁迫效应仍然缺乏系统的研究。本研究以辽宁省普遍种植的四种大白菜(抗病金春KB、东洋春夏DY、青绿王QL、强势QS)为对象,用发芽实验、砂培实验和盆栽实验,研究了Cd、Pb单一及复合胁迫下大白菜的生理生化变化。 在发芽阶段,Cd、Pb单一及复合胁迫下,重金属浓度与根长、芽长和生物量抑制率间呈极显著线性相关,根伸长对Cd、Pb的毒害最敏感。KB对Cd毒害的抗性最强,DY对Pb单一和Cd、Pb复合胁迫的抗性最强,而QS对Cd、Pb单一及复合胁迫的抗性最弱。Cd、Pb复合胁迫时,对根伸长、芽伸长和生物量抑制的联合作用类型都为相加作用。 砂培实验中,在Cd、Pb浓度较低时,4种大白菜超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性及丙二醛(MDA)、可溶性蛋白(SP)、脯氨酸(PRO)含量都不同程度提高,但是随Cd、Pb浓度的提高,各生理过程受到抑制。KB和QS中PRO的含量约是DY和QL中的2-3倍,高PRO累积量很可能是Cd、Pb耐性大白菜品种所具有的特点。 盆栽实验中,在各生长时期,抗氧化酶活性、抗坏血酸(AsA)和还原型谷胱甘肽(GSH),及可溶性糖和SP的含量随Cd、Pb浓度的提高先升高后下降。硝态氮的含量基本随Cd、Pb浓度的提高而上升。在整个实验期间,KB和QS中MDA都保持较低的水平,KB对Cd、Pb的耐性主要是由于其体内PRO及AsA、GSH等抗氧化剂的累积;而GSH和可溶性糖对QS的Cd、Pb耐性起重要作用。QL和DY中MDA的含量要高于KB和QS,它们体内的抗氧化酶活性,及PRO、AsA和GSH含量在某个生长时期会达到较高的水平,但是与KB和QS相比,这些变化并不稳定,所以比KB和QS对Cd、Pb的耐性要弱。

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随着工农业的快速发展,土壤重金属尤其Cd和Pb污染日益严重。筛选和培育具有重金属低积累特性的农作物排异品种被认为是当前应对土壤重金属污染最为合理和有效的途径之一。本文通过盆栽试验、大田试验和砂培试验,研究了大白菜品种对Cd和Pb的吸收和积累的品种差异、对Cd、Pb胁迫的响应以及大白菜安全生产的调控技术,得出以下结论: 1) 盆栽梯度试验中,80种大白菜地上部对Cd的吸收存在显著差异(p < 0.05)。在3种Cd处理下(1.0, 2.5和5.0 mg/kg),80种大白菜Cd含量浓度范围分别为(mg/kg) 0.22–2.46, 0.90–14.10和2.03–18.01, 其平均值分别为 0.79, 3.76 和6.79 mg/kg DW。大白菜对Cd胁迫具有较强的耐性。大田试验中,15种大白菜的富集系数和转运系数与盆栽梯度试验的结果基本一致。排异植物的筛选和鉴定标准包括:(1)该植物的地上部和根部的Cd含量都很低或者可食部位低于有关标准;(2) 富集系数(BF) < 1.0;(3) 转运系数(TF) < 1.0;(4)该植物具有较高的Cd耐性,在较高的Cd污染下能够正常生长且生物量无显著下降。采用此标准,结合盆栽梯度试验和大田试验结果,北京新3号、绿星70和丰源新3号可鉴定为Cd排异品种。秋傲和赛新5号具有排异Cd特性,但其对Cd的耐性较差。 2) 盆栽梯度试验中,在Pb投加浓度为500和1500 mg/kg处理下,30种大白菜地上部对Pb的吸收存在显著差异 (p < 0.05),其Pb浓度的范围分别为:0.52–8.68 和1.86–16.20 mg/kg, 其平均值分别为3.01 和6.87 mg/kg DW。并且,随着Pb浓度的增加,白菜地上部Pb含量有随之增加的趋势。大白菜对Pb具有较强的耐性。低浓度的Pb处理对大白菜的生物具有一定的促进作用。结合盆栽试验和大田试验的结果,秋傲、世博秋抗和福星80可鉴定为Pb排异大白菜品种。 3) 砂培试验中,在Cd和Pb胁迫下,大白菜地上部的丙二醛(MDA)含量增加,随着Cd处理浓度的增加,超氧化物歧化酶(SOD)活性呈现先下降后上升接着下降的趋势,酸菜王的SOD活性要高于北京新3号SOD的活性。随着Pb处理浓度的增加,福星80地上部的SOD活性随之增加,而绿星大棵菜地上部的SOD活性先下降后增加。在不同梯度的Cd处理下,大白菜地上部的可溶性蛋白(SP)含量未见显著降低,甚至有所增加,而在不同梯度的Pb处理下,大白菜地上部的SP含量有所降低。 4) 施用改良剂可升高土壤的pH值和降低土壤中的有效态Cd,从而对大白菜的生长具有促进作用。施用改良剂可显著降低大白菜对Cd和Pb的吸收和累积。