999 resultados para Inferência de atividades


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Wireless sensor networks (WSN) have gained ground in the industrial environment, due to the possibility of connecting points of information that were inaccessible to wired networks. However, there are several challenges in the implementation and acceptance of this technology in the industrial environment, one of them the guaranteed availability of information, which can be influenced by various parameters, such as path stability and power consumption of the field device. As such, in this work was developed a tool to evaluate and infer parameters of wireless industrial networks based on the WirelessHART and ISA 100.11a protocols. The tool allows quantitative evaluation, qualitative evaluation and evaluation by inference during a given time of the operating network. The quantitative and qualitative evaluation are based on own definitions of parameters, such as the parameter of stability, or based on descriptive statistics, such as mean, standard deviation and box plots. In the evaluation by inference uses the intelligent technique artificial neural networks to infer some network parameters such as battery life. Finally, it displays the results of use the tool in different scenarios networks, as topologies star and mesh, in order to attest to the importance of tool in evaluation of the behavior of these networks, but also support possible changes or maintenance of the system.

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This study aimed to extract, characterize and conduct a prospective analysis of pharmacological activities of sulfated polysaccharides from green seaweed Caulerpa prolifera. Seven fractions (CP-0.3/CP-0.5/CP-0.7/CP-0.9/CP-1.1/CP-1.5/CP-2.0) were obtained from C. prolifera by alkaline proteolysis followed by sequential precipitation in acetone. The physicochemical analyzes indicated that C. prolifera synthesizes a homogalactan (CP-0.9) and different populations of sulfated heteropolysaccharides. In the analysis of anticoagulant activity, all fractions except CP-0.3, influenced the intrinsic coagulation pathway. All fractions showed antioxidant activity in six different assays being more pronounced in hydrogen peroxide scavenging assay, especially CP-0.3, CP-0.7 and CP-0.9 (which obtained 61% of hydrogen peroxide scavenging), in ferric chelation assay (especially CP-0.9 with 56% chelation) and cupric chelation assay (especially CP-2.0 with 78% chelation). With respect to immunomodulatory activity, the presence of CP-0.3, CP-0.7 and CP-0.9 showed an immunogenic potential, increasing the production of nitric oxide (NO) by 48, 142 and 163 times, respectively. Conversely, the NO synthesis fell 73% after the activation of macrophages by LPS, incubated concurrently with CP-2.0. The anti-adipogenic activity of the fractions was also evaluated and CP-1.5 was able to reduce the differentiation of pre-adipocytes (3T3-L1) into adipocytes by 60%, without affecting the cell viability. The fractions CP-0.3, CP-0.5 and CP-0.9 reduced the viability of the HeLa cells (human cervical adenocarcinoma) by 55% and CP-1.5 reduced the viability of the 786-0 cells (human renal adenocarcinoma) by 75%. Leishmanicidal activity and microbicide effect against Carbapenem-resistant Klebsiella pneumoniae (KPC) have not been identified. However, the viability of Staphylococcus epidermidis was reduced by 23.8% in the presence of CP -1.5. All fractions were able to change the formation of calcium oxalate crystals. CP-0.3, CP-0.5 and CP-1.1 only promoted the formation of COD type crystals with a very small size (1 μm). Confocal microscopy and zeta potential data of crystals formed in the presence of the samples showed that the polysaccharides present in the fractions must interact with calcium ions present throughout the crystal lattice, affecting the growth and morphology of crystals The results described herein indicate that the fractions rich in polysaccharides obtained from the green seaweed C. prolifera present a multi therapeutic potential, and subsequent purification steps, as well as research on the mechanisms of action by which these polymers act should be investigated.

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Marine algae are rich sources of various structural compounds which recently has been increasingly studied as a new source of bioactive substances. The alginate, as come as fucans, are considered the main acidic polysaccharides found in brown seaweed. This molecule consists a linear natural polysaccharide, non-sulfated, and presents monosaccharides: acid β-D-mannuronic (M) and α-L-guluronic acid (G); in a vast amount compositions and threads. Alginate has been widely applied in food and pharmaceutical industries because of its ability to retain water, forming films and gels as well as thickening, stabilizing and form emulsions. In this work we aimed to extract, structurally characterize, compare and analyze the possible pharmacological activities of native alginate molecule obtained from brown seaweed Dyctiopteris delicatula (DYN), and its chemically sulfated derivative (DYS). The alginate structure and composition molecule can be proven through chemical dosing, that showed low protein contamination and high sugar level, existence and separation of M and G blocks in the descending paper chromatography, infrared spectroscopy and nuclear magnetic resonance. Molecule sulfation was proven with sulphate dosage, resulting in 28.56% sulphate in molecule; electrophoresis, verify metachromasia with toluidine blue; and infrared spectroscopy, that showed a characteristic band at 1221cm-1 corresponding a sulfate group vibration. For the pharmacological activities the tests was: antioxidant activity, changes in cell function (MTT test) and anticoagulant test. In the antioxidant activity we observed that DYN showed better results in the kidnapping of hydroxyl radicals and ferric chelation compared to DYS, this had the best result in the total antioxidant capacity. Both showed similar activity in reducing power and the kidnapping radicals DPPH. In MTT test DYN and DYS had not proliferative and cytotoxic activity in fibroblast cells (3T3) and showed antiproliferative and cytotoxic activity in cancer cell lines HeLa and B16 melanoma. In anticoagulant assay DYN showed good activity in the intrinsic pathway of blood coagulation, and a small activity in the extrinsic pathway, in the other hand DYS showed only a very small activity in the extrinsic pathway, but cannot come to be regarded as an anticoagulant agent. From these results it can be concluded that the alginate was extracted and sulfated, revealing a potential compound to be used in the pharmaceutical industry as an anticoagulant agent, antioxidant and antitumor and the sulfation has not been conclusively important to performance in the tested pharmacological activities

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Fucoidan is a term used to define heteropolysaccharides that are composed of less than 90% L-fucose. The exception to this rule is the homofucoidan obtained from the seaweed Fucus vesiculosus. This fucoidan can be purchased from SIGMA Co. and have been used in various research for evaluation of their pharmacological activities. However, it is not a pure molecule. In fact, it is a mix of several fucoidan molecules. In this work, were obtained, from acetone precipitation, and biochemically characterized, four fucoidan molecules from SIGMA-ALDRICH Co. fucoidan to evaluate their anticoagulant, antioxidant, antiadipogenic, immunomodulatory and antiurolithiatic activities. In anticoagulant activity, evaluated by aPTT assay, fucoidans F0.9, F1.1 and F2.0 increased eightfold the coagulation time, compared to the control, when a mass of 10 μg was used. To PT test, only fucoidan F0.9 was capable of increase the coagulation time, compared to control. In the total antioxidant capacity assay (TAC), the fucoidan F2.0 showed 400 ascorbic acid equivalents, while fucoidan F0.5, the lest effective, 38 equivalents. In respect to the effect on pre-adipocyte cell lines (3T3-L1) adipogenesis, was observed that fucoidan F1.1 and F2.0 reduced the adipogenesis and this effect was associated to the reduction in the expression of regulatoy proteins C/EBPα, C/EBPβ and PPARγ. On the other hand, fucoidans F0.5 and F0.9 induced increased expression of these regulatory proteins. Furthermore, fucoidan F2.0 induced hydrolysis of triglycerides present in the interior of adipocytes. The immunomodulatory effect was evaluated and observed that the presence of fucoidans F0.5 , F1.1 and F2.0 significantly reduced the production of nitric oxide by activated macrophages with LPS specially fucoidan F2.0 that in 100 μg/mL, reduced about 55% the effect caused by LPS. Relative to the effect upon the formation of calcium oxalate crystals, fucoidan F0.5 was more effective in reduce the aggregation of the crystals and this effect it was not significantly different regarding the effect caused by the crude. Besides, fucoidan F0.5 only promoted the formation of COD type crystals, while fucoidans F1.1 and F2.0 did not influence the formation of crystals compared with the control. The results described in this study indicate that the commercial crude fucoidan of Fucus vesiculosus it’s a mix of several fucoidan which, in turn, have different chemical compositions besides having different pharmacological activities. The use of these fucoidans it´s indicated according the pharmacological activity to be evaluated.

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Fucoidan is a term used to define heteropolysaccharides that are composed of less than 90% L-fucose. The exception to this rule is the homofucoidan obtained from the seaweed Fucus vesiculosus. This fucoidan can be purchased from SIGMA Co. and have been used in various research for evaluation of their pharmacological activities. However, it is not a pure molecule. In fact, it is a mix of several fucoidan molecules. In this work, were obtained, from acetone precipitation, and biochemically characterized, four fucoidan molecules from SIGMA-ALDRICH Co. fucoidan to evaluate their anticoagulant, antioxidant, antiadipogenic, immunomodulatory and antiurolithiatic activities. In anticoagulant activity, evaluated by aPTT assay, fucoidans F0.9, F1.1 and F2.0 increased eightfold the coagulation time, compared to the control, when a mass of 10 μg was used. To PT test, only fucoidan F0.9 was capable of increase the coagulation time, compared to control. In the total antioxidant capacity assay (TAC), the fucoidan F2.0 showed 400 ascorbic acid equivalents, while fucoidan F0.5, the lest effective, 38 equivalents. In respect to the effect on pre-adipocyte cell lines (3T3-L1) adipogenesis, was observed that fucoidan F1.1 and F2.0 reduced the adipogenesis and this effect was associated to the reduction in the expression of regulatoy proteins C/EBPα, C/EBPβ and PPARγ. On the other hand, fucoidans F0.5 and F0.9 induced increased expression of these regulatory proteins. Furthermore, fucoidan F2.0 induced hydrolysis of triglycerides present in the interior of adipocytes. The immunomodulatory effect was evaluated and observed that the presence of fucoidans F0.5 , F1.1 and F2.0 significantly reduced the production of nitric oxide by activated macrophages with LPS specially fucoidan F2.0 that in 100 μg/mL, reduced about 55% the effect caused by LPS. Relative to the effect upon the formation of calcium oxalate crystals, fucoidan F0.5 was more effective in reduce the aggregation of the crystals and this effect it was not significantly different regarding the effect caused by the crude. Besides, fucoidan F0.5 only promoted the formation of COD type crystals, while fucoidans F1.1 and F2.0 did not influence the formation of crystals compared with the control. The results described in this study indicate that the commercial crude fucoidan of Fucus vesiculosus it’s a mix of several fucoidan which, in turn, have different chemical compositions besides having different pharmacological activities. The use of these fucoidans it´s indicated according the pharmacological activity to be evaluated.

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Relatório de estágio apresentado para obtenção do grau de mestre em Ensino do 1º e do 2º ciclos do ensino básico

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A busca por soluções para o apoio às atividades de ensino faz parte da rotina dos educadores em todo mundo. Com a redução dos custos dos equipamentos eletrônicos pessoais, ampliação no acesso à Internet e o constante surgimento de novos aplicativos, amplia-se o interesse por ferramentas eletrônicas que possam incrementar as atividades didáticas. Nessa publicação, apresentamos uma coleção de recursos que mereceram destaque nas atividades de treinamento conferidas pela equipe técnica do NEAD.TIS - FMB UNESP e colaboradores no ano de 2015. A escolha foi baseada em alternativas gratuitas, criativas, com acesso fácil, baixa complexidade de uso e que apresentassem grande potencial para incorporação natural às atividades dos professores de diversas especialidades. Nossa publicação não tem a intenção de esgotar as incontáveis alternativas que surgem diariamente e nem de abordar detalhes técnicos de cada um dos recursos apontados, mas sim de apresentar um conjunto básico de ferramentas inovadoras e flexíveis, que podem ser exploradas em diferentes projetos e cenários de formação acadêmica e profissional. Esperamos que todos apreciem o potencial de cada recurso e que compartilhem suas experiências entre a comunidade acadêmica.

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O presente relatório, elaborado para fins de conclusão do Mestrado em Ensino do 1.º e do 2.º Ciclo do Ensino Básico, encontra-se organizado em duas dimensões: a dimensão reflexiva e a dimensão investigativa. A primeira é composta por uma reflexão crítica relativas a aspetos mais significativos da Prática Pedagógica, ao longo de quatro semestres, e que me permitiram evoluir enquanto professora. A segunda dimensão, a investigativa, centra-se num estudo realizado em 1.º Ciclo do Ensino Básico, tendo como participantes três alunos do 3.º ano de escolaridade. A presente investigação, de caráter qualitativo, procurou dar resposta à questão: Qual o contributo da implementação de atividades práticas e experimentais sobre sombras no desenvolvimento: a) dos conhecimentos científicos de alunos do 3.º ano de escolaridade; e b) dos processos da ciência em alunos do 3.º ano de escolaridade? Os dados recolhidos mostram que a implementação de atividades práticas e experimentais poderão ter influenciado positivamente o desenvolvimento das aprendizagens dos alunos sobre sombras e a mobilização de processos da ciência.

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O presente relatório integra o Mestrado em Educação Pré-Escolar e Ensino do 1.º Ciclo do Ensino Básico e apresenta-se constituído por duas dimensões: a reflexiva e a investigativa. A dimensão reflexiva é a primeira dimensão do relatório que se estrutura em três partes fundamentais. A primeira e a segunda partes incluem uma reflexão crítica sobre as práticas pedagógicas desenvolvidas em contexto de Educação de Infância e em 1.º Ciclo do Ensino Básico. A terceira refere-se aos papéis reflexivo e investigativo do educador e do professor, transversais aos dois contextos. A dimensão investigativa é a segunda dimensão do relatório, na qual se apresenta um estudo de caso realizado com cinco crianças do 3.º ano de escolaridade de uma escola dos arredores da cidade de Leiria. A investigação procurou resposta para a seguinte questão “Quais as perceções das crianças do 3.º ano de escolaridade sobre o seu desempenho nas atividades de interpretação musical, realizadas em contextos de sala de aula?”. Numa primeira fase, realizou-se a caracterização das experiências de participação musical da turma através de um inquérito por questionário. Foram utilizadas entrevistas semiestruturadas, de caráter individual, antes e após a concretização de duas sequências de atividades para todos os alunos da turma. Os resultados obtidos sugerem que existem algumas alterações nas perceções dos aluno e que as propostas educativas implementadas com as crianças influenciaram positivamente as suas perceções relativamente à interpretação de canções.

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A dissertação que aqui se apresenta, de cariz qualitativo, procura perceber quais os contributos que as atividades de animação sociocultural desempenham para os idosos de Serviço de Apoio Domiciliário (SAD) da Santa Casa da Misericórdia de Pombal (SCMP), uma resposta social que oferece, apenas, serviços de higiene habitacional e pessoal, alimentação, tratamento de roupas e cuidados de saúde aos seus utentes. Animação Sociocultural na terceira idade surge como resposta à diminuição da sua atividade e das suas relações sociais. É considerada um fator determinante para a qualidade de vida das pessoas preservando a sua autonomia e podendo contribuir para um envelhecimento mais ativo e participativo. O método usado foi a investigação ação, com 13 utentes do serviço de apoio domiciliário, desenvolvendo-se um conjunto de atividades, previamente escolhidas por eles, como a expressão plástica, tertúlias, ginástica, caminhadas, manicure, jogos de cartas, leitura de jornais e a comemoração do aniversário. As técnicas que nos permitiram proceder à recolha de dados foram a pesquisa documental, a entrevista estruturada e a observação direta com recurso ao diário de campo. A pesquisa desenvolvida evidenciou que os utentes mostram maior interesse em desenvolver atividades que envolvam o diálogo, sobre assuntos do quotidiano pessoal e da atualidade portuguesa, e a atividade física. Percebeu-se que as atividades de animação socioculturais desenvolvidas trouxeram contributos a nível da mobilidade, das relações pessoais, da auto estima, da motricidade fina, entre outras. De futuro consideramos que as atividades de animação sociocultural devem ser um serviço a incluir na resposta social do serviço de apoio domiciliário.

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TORRES, Gilson de Vasconcelos; ENDERS, Bertha Cruz. Atividades educativas na prevencao da AIDS em uma rede basica municipal de saude: participacao do enfermeiro. Rev.latino-am.enfermagem, Ribeirao Preto, v.7, n.2, p.71-77, abril 1999. Disponivel em: . Acesso em: 04 set. 2010.

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As organizações de alta confiabilidade são caracterizadas pelo alto nível de complexidade e de exposição contínua a riscos. Sob esse ambiente, observa-se o surgimento de estresse e de conflitos interpessoais que, para adequada gestão, demandam habilidades de comunicação. Esta pesquisa tem por objeto de estudo a comunicação documentada nos casos de incidentes e acidentes ocorridos na Aviação Civil, investigados pela Federal Aviation Administration e pelo Centro de Investigação e Prevenção de Acidentes Aeronáuticos. De acordo com dados das agências, deficiências na comunicação respondem por aproximadamente 80% das ocorrências. Contudo, os resultados da pesquisa, confirmam não haver carga horária suficiente de treinamento de habilidades de comunicação para atendimento específico de situações de extremo risco e de contínua pressão. O treinamento é mais focado em habilidades técnicas do que em habilidades sociais.