角蟾科（Megophryidae; Anura）蝌蚪的形态多样性和生态适应

角蟾科（Megophryidae）是以角蟾属（Megophrys Kuhl and Van Hasselt, 1822）为模式属而建立的，隶于无尾目（Anura），变凹型亚目（Anomocoela）。角蟾科包括2 亚科11 属142 种，分布于东洋界，从巴基斯坦、中国西部向东直到菲律宾和苏达群岛；中国有9 属75 种分布于华中和华南地区。角蟾科被认为是原始的两栖动物之一，其分类学、系统学、生态学、动物地理学的研究均深受中外科学家的瞩目。近年来，通过形态学、古生物学、细胞学、生态学、支序系统学的研究，角蟾科的分类与系统学研究取得了较大进展。与成体形态和分子系统学研究结果相比较，蝌蚪的研究存在更多的问题和挑战，尚需深入研究：（1）角蟾科蝌蚪的形态多样性分析；（2）角蟾科的系统发育关系与蝌蚪的演化，以及口漏斗的起源；（3）角蟾科蝌蚪表型分化与栖息环境和觅食行为的适应演化。针对上述问题，本文对角蟾科9 属30 种蝌蚪的形态特征，包括外部宏观形态和口器外部结构特征、口器内部显微结构、唇齿和角质颌的亚显微结构作了深入细致、多层次的比较研究；通过12s rRNA 和cytochrome b 基因构建最大简约树，采用贝叶斯系统发育进行分析，蝌蚪型的演化采用祖先性状的重建方法分析；得到如下结论：1）初步将角蟾科蝌蚪分为4 种类型；并且建立了2 种新的角蟾科蝌蚪类型。A 型：拟髭蟾型蝌蚪，该型蝌蚪包括拟髭蟾属、髭蟾属、齿蟾属和齿突蟾属的物种；B 型：新类型，掌突蟾型蝌蚪，该型蝌蚪在本文中包括掌突蟾属、小臂蟾属的物种；C 型：新类型，短腿蟾型蝌蚪，一种特化类型，该型蝌蚪在本文中仅包括短腿蟾属的物种；D 型：角蟾型蝌蚪，该型蝌蚪在本文中包括无耳蟾属、小口拟角蟾属和异角蟾属的物种。2）对角蟾科的分类进行了修订：（1）支持角蟾科两个亚科的分类系统；（2）角蟾亚科包括拟角蟾属、异角蟾属、无耳蟾属和短腿蟾属；该亚科形态差异小，系统学关系比较复杂，暂不作族级分类的再划分；（3）拟髭蟾亚科分为2 个族：拟髭蟾族，该族物种具有类型A 的蝌蚪，包括4 个属：拟髭蟾属、髭蟾属、齿蟾属、齿突蟾属；掌突蟾族，该族物种具有类型B 的蝌蚪，包括2 个属：掌突蟾属和小臂蟾属。3）结合分子系统进化关系探讨了4 种蝌蚪类型的演化。（1）角蟾科蝌蚪的最近共同祖先来自于一类具有拟髭蟾型蝌蚪性状的蝌蚪；（2）掌突蟾型蝌蚪和角蟾亚科的蝌蚪是由具有拟髭蟾型蝌蚪性状的祖先蝌蚪分别演化而来；（3）短腿蟾型蝌蚪是角蟾型蝌蚪的一种特化类型；（4）外群蝌蚪具有与拟髭蟾型蝌蚪相似的性状，进一步印证了类拟髭蟾型蝌蚪是角蟾科蝌蚪的最近共同祖先的假说；（5）具有口漏斗的蝌蚪类型是由不具口漏斗的蝌蚪类型演化而来，在角蟾科中口漏斗是一种衍生性状。4）分析了角蟾科四种蝌蚪类型与栖息环境的适应演化。（1）角蟾科蝌蚪的口部和体形的变化反映了该科蝌蚪由缓流向类似静水生境的回水凼的渐变式适应，角蟾科蝌蚪的形态显示了多方面的适应变化；（2）随着蝌蚪类型由A 向D的演化，当水速较大时，拟髭蟾型的蝌蚪营流水攀吸型生活方式；当水速递减时，掌突蟾型蝌蚪营流水附着型生活方式；当水速进一步递减时，具有较小口漏斗的短腿蟾型蝌蚪和具有大漏斗的角蟾型蝌蚪营流水浮泳型生活。角蟾科蝌蚪对于水流递减的适应演化说明蝌蚪的生态学适应是具有进化意义的；（3）蝌蚪口器内部结构的分化揭示了蝌蚪和食性的适应关系，蝌蚪以口部的唇齿与角质颌刮取或吞吸水中的物质，然后，通过口乳突有选择地过滤进入口腔中食物。拟髭蟾亚科蝌蚪的唇齿多而窄，唇齿间距宽，颌鞘粗而稀，反映了其植食性为主的特点；它们的舌前乳突一般为指状，在口腔入口处所占面积小，其机械过滤的作用很多被唇齿和角质颌分担了；而角蟾亚科的蝌蚪，其角质颌弱，其舌前乳突一般为匙状，几乎填满了口腔入口处，因此舌前乳突起了主要的机械过滤作用。The family Megophryidae is the largest and most diverse families inArchaeobatrachia, and most of its species occur in India, Pakistan, and eastward intoChina, Southeast Asia, Borneo and the Philippines to the Sunda Islands. Currently thefamily includes 142 species have been grouped into two subfamilies, Megophryinaeand Leptobrachiinae. The mountains of central and southern China are rich in speciesof Megophryidae, 75 species belong to 9 genera and two subfamilies.The family was supposed to be ideal materials of studies in many fields of biology,such as taxonomy, evolution, systematics, ecology, and biogeography. Recently, therehave a great development in taxonomy and systematics of megophryids throughstudied by morphology, paleontology, cytology, ecology, and cladistics. However,larvae of megophryids were generally unknown, although the tadpoles might be veryimportant for above studies.In this paper, we examined the evolutionary scenario of the tadpoles’ morphologyin the context of a phylogenetic framework. Our objectives are (1) to evaluate thedivergence of larval body shape and oral discs in the family Megophryidae, (2) toexplore the evolutionary trends of the larvae in megophryidae, and test if thefunnel-shaped oral disc is apomorphic, and (3) to explore the relationship of the larvalstructure, diet and microhabitat.We examined larval morphology of 30 megophryid species, the larval body shape,oral discs, the buccopharyngeal cavity, and jaw sheaths and denticles of the Chinesemegophryid frogs were re-examined. We constructed a phylogeny of the species on thebasis of published mitochondrial cytochrome b and 16S rRNA gene segments usingpartitioned Bayesian analyses. Furthermore, hypothetical changes of larval morphologywere inferred using parsimony principle on the phylogeny. The results showed that:1) Four tadpole types in Megophryidae. The larval morphological charactersseries in Chinese megophryids fall into four general categories according to the bodyshape and oral discs: (A) Leptobrachiini type, species from genera Leptobrachium,Oreolalax, Scutiger and, Vibrissaphora share this type of tadpoles. (B) Leptolalax type,species of genus Leptolalax have this type of tadpoles. (C) Brachytarsophrys type,species of the genus Brachytarsophrys have this type of tadpoles. (D) Megophryinitype, species of the genera Atympanophrys, Ophryophryne, and Xenophrys share this type of tadpoles. Of which B and C are two novel types.2）Taxonomic implications. The present study leads us to reconsider the generalclassification of tribes attributed to members of Megophryidae. More specifically,concerning the phylogenetic relationships and the two novel tadpole types describedherein, we propose a provisional taxonomy for the family but suggest that further taxasampling of other megophryids be performed to confirm this taxonomic change. TheMegophryidae is composed of two subfamilies (Leptobrachiinae and Megophryinae).The Leptobrachiinae was recogonized the two tribes: (1) tribe Leptobrachiini sensuDubois, corresponding to the tadpole of type A, including four genera, i.e.,Leptobrachium, Oreolalax, Scutiger and, Vibrissaphora; (2) tribe Leptolalaxini,corresponding to the tadpole of novel type B, including two genera, i.e., Leptolalaxand Leptobrachella. However, the relationships among the genera of Megophryinaewere largely unresolved, they recognized no monophyletic groups above the generalevel. A more thorough sampling will likely foster a better taxonomic solution.3) The larval evolutionary scenario in Megophryidae.Type A is characteristicof normal-mouthed with multiple tooth rows, representing the tadpole type of theMRCA of Chinese megophryids. Type B is characteristic of normal-mouthed withreduced tooth rows, prolonging labium, and integumetary glands. Type C ischaracteristic of no labial teeth and smaller umbeliform oral disc. Type D ischaracteristic of no labial teeth, enlarged umbeliform oral disc, representing the tadpoleof the MRCA of subfamily Megophryinae. A previous hypothesis, referring tofunnel-shaped oral discs as an apomorphy, is supported.4) The larval adaptation to habitats in Megophryidae. Tadpoles generallyadhere to substrates using their mouths, and the microhabitat that the tadpoles occupyreflects the degree of adhesion and oral complexity. The morphological changes inmegophryid tadpoles virtually allow a progressive adaptation to a changing habitatfrom faster water to slower water. Within the tadpoles of Type A to type D, the TOTbecomes smaller and smaller, and the oral disc orientates from anteroventral toumbelliform upturned, and eye position orientates from dorsal to lateral, and the trunkis more and more depressed and tail becomes relatively longer and slender. Within therunning water, the normal-mouthed with multiple tooth rows of Leptobrachiini tadpoles are correlated with lotic-suctorial, benthic feeders with anteroventral oraldisc and the largest body. With the water’s velocity decreasing, the lotic-adherentfeeders of Leptolalax tadpoles have tube-shaped labium with reduced tooth rows andintegumetary glands. And then, the smaller umbeliform in Brachytarsophrys tadpolesand the enlarged umbeliform oral disc in the Megophryini tadpoles are inhabitmicrohabitats of non-flowing backwaters of rivers, indicative of adaptive traits oflotic-neustonic surface feeders. The scheme of megophryid tadpoles andmicrohabitats provided the first clear evidence which congruent with the hypothesis ofAltig and Johnston (1989). The ecological divergence plays a general role in thedivergence and evolution of megophrid larvae. There is a definite correlation amongthe buccopharyngeal cavity, diet and feeding mechanisms, the tadpole graze orswallow the food particles, then through papillae which like a sieve and sort out foodparticles to the oesophagus. The tadpole of Leptobrachiinae possess multiple toothrows, wide intertooth distance as well as thick and sparse jaw sheath, these tadpolesinhabit bottom of the streams and graze on epiphyton or major detritus of organicmatter on the substrates, their prelingual papillae like single finger, the mechanicalpurpose of papillae served share in by tooth and jaw. The tadpoles of Megophryinaeoccur near the water surface of small streams and are the filter feeder, their dietincludes plankton and organic debris floating on the water surface, those tadpolepossess weak jaw, their prelingual papillae like spoon, the mechanical purpose ofpapillae served mostly for sieve.

HIRFL-CSR前端总线控制器的SD驱动

The HIRFL-CSR EVME bus controller, which runs Embedded Linux OS, is based on AT91RM9200 microprocessor, whose core is ARM920T. There are hardware interface electronic circuits connecting AT91RM9200 microprocessor and Security Digital Memory Card (SD Card). This article analyzes Operation System kernel and Linux device driver’s structure, designs SD Card driver based on Embedded Linux, which runs on AT91RM9200 microprocessor.中文文摘：简要论述了用于兰州重离子加速器冷却储存环(HIRFL-CSR)控制系统的前端总线控制器。该控制器是基于ARM920T核心的AT91RM9200处理器,运行嵌入式Linux操作系统。描述了AT91RM9200处理器与Security Digital MemoryCard(SD卡)的硬件接口电路,分析了操作系统内核和Linux驱动程序结构,设计和实现了嵌入式Linux下基于AT91RM9200处理器的SD卡驱动程序。

基于TCP/ IP 协议的CSR离子泵电源远程监控软件系统设计

This paper discuss a Ion-pump Power Supply control system making use of RS232 series bus and Intranet.The system s hardware VAC800 is composed of MSP430F149 mixed-signal processors produced by TI and UA7000A network model.MSP430F149 has advantages of ultra-low-power and high-integration.The Ion-pump Power Supply control system has the characteristics of strong function,simple structure,high reliability,strong resistance of noise,no peripheral chip,etc.Visual studio 2005 is used to design the system s softwa...中文文摘：论述了通过RS-232总线和Intranet网络,来实现对远端的离子泵电源的监测与控制。系统硬件VAC800由TI公司的MSP430F149混合信号处理器和UA7000A网络模块构成。MSP430F149具有超低功耗和高集成度等优点,利用它构建的离子泵电源监控系统功能强大,结构简单,可靠性高,抗干扰能力强。系统软件采用visual studio 2005设计。本监控系统能够很好地完成对加速器离子泵电源监视与控制。

SFC200KW高频发射机控制系统改造

本文全面论述了一个基于PROFIBUS现场的总线分布式控制系统的设计、开发、调试过程，详细介绍了控制系统的结构和软硬件设计。论文从SFC200KW高频发射机控制需求出发，阐明了控制系统的开发背景、基本结构的建立、硬件接口电路的设计方案、控制算法的设计思想以及上下位机控制程序的实现。论文分四大部分，首先介绍SFC200KW高频发射机控制系统的现状，阐明进行SFC200KW高频发射机控制系统改造的必要性和采用现场总线分布式控制系统的优势。第二部分介绍控制系统改造的相关技术。第三部分结合PROF工BUS和FCS控制系统理论详细介绍了新SFC200KW高频发射机控制系统的结构，包括主控器PLC实时控制、系统抗干扰特性、电平转换接口等。第四部分结合SFC200KW高频发射机控制算法，下位机PLC编程语言，介绍了控制系统软件的设计和开发。上位机监控程序采用W工NCC组态软件完成友好的人机交互界面开发。通过对该控制系统的改造，满足了工程对控制部分可靠性、实时性和稳定性的需求。实现了对SFC200KW高频发射机远程控制，运行参数实时采集，故障实时保护和报警记录，运行参数归档和打印等功能。达到了改造的目的。

总线控制器的设计及其在CSR控制系统中的应用

HIRFL-CSR(兰州重离子冷却储存环)是国家“九五”重大科学工程之一。CSR控制系统是保证CSR正常运行的重要环节。这是一个基于以太网的分布式控制系统，它由两部分组成：总体控制服务器系统和前端控制服务器系统。 本文介绍了应用于CSR前端控制系统中的嵌入式总线控制器。首先介绍了嵌入式总线控制器的硬件设计方案。系统以32位高速双以太网处理器JUPITER为核心。JUPITER是一款低成本、高性能、建立在以太网系统基础之上的处理器。该系统的外阴电路主要包括存储模块、接日模块、总线控制模块。接口模块有RS485、RS232和网络接口电路。其次论述了嵌入式操作系统uClinux的体系结构以及uClinux与标准Linux的不同之处。论文从uClinux的内核结构出发，讨论了源代码的组织结构，概括分析了uClinuxlj勺内存管理、进程管理。最后介绍了uClinujx统的移植方法和应用实现。uClinux移植主要包括引导装载程序、uClinux内核和文件系统三个部分。在引导装载程序的移植过程中，论文重点给出了一种固化引导装载程序的方法；在uClinux内核移植中，讨沦了交叉编译环境l为建立和uClinux内核配置方法，总结了uClinux内核移植的层次结构和具体的移植过程。沦文通过对嵌入式处理器的分析，以及对嵌入式操作系统移植的探讨，展示了嵌入式系统开发的核心技术，对眠入系统的开发应用具有一定的实际意义。

细沟侵蚀过程与细沟水流水力学参数的关系研究

利用供沙土槽和试验土槽的双土槽径流小区 ,定量研究了在不同降雨强度下上方来水来沙对陡坡地细沟侵蚀产沙过程和细沟水流水力学参数的影响及其细沟水流水力学特征参数与细沟侵蚀产沙量的关系。结果表明 :坡面细沟侵蚀过程以侵蚀—搬运过程为主 ,坡上方来沙不仅被径流全部搬运 ,且上方来水在坡下方引起了另外的侵蚀产沙量 ,其值随上方来水含沙量的减少和降雨强度的增加而增大。上方来水的汇入或降雨强度的增大可使细沟水流流态由层流转化为紊流。上方来水对细沟水流水力学参数 (流速、水力半径、雷诺数、弗劳德数和阻力系数 )有重要影响。定量分析了细沟水流水力学特征参数 (流速、雷诺数和阻力系数 )与上坡来水引起坡下方净侵蚀产沙量的关系 ,建立了净侵蚀产沙量与细沟水流流速、雷诺数和阻力系数统计模型。

上方来水来沙对细沟侵蚀产沙过程的影响

利用双土槽系统径流小区 (供沙土槽和试验土槽 ) ,定量研究了不同上方来水含沙量和不同降雨强度下 15°坡面上方来水来沙对坡下方细沟侵蚀产沙过程的影响。结果表明 ,坡上方来沙量不但被径流全部搬运 ,且坡上方来水在坡下方细沟侵蚀槽引起另外的侵蚀产沙量 S。坡面细沟侵蚀过程以侵蚀—搬运过程为主。上方来水对细沟侵蚀产沙的贡献受上方来水含沙量和降雨强度的影响。降雨强度的增加或上方来水含沙量的减少 ,使 S值的增加更为显著

海洋要素垂直剖面测量系统的数据存储

长期、定点、连续测量海洋环境参数，尤其是从海洋表面到海底的垂直剖面的监测一直以来大都是由船只完成，耗费大量的人力和财力。“海浪驱动自持式海洋要素垂直剖面测量系统”（简称“海马”）利用海洋无时不在的波浪能，驱动滑行器单方向下潜运动，直达海底或程序设定的预定深度，在控制系统作用下，滑行器依靠自身的浮力匀速上浮， 并在此过程中，完成海洋要素的自动测量和存储。 当今海洋仪器大多采用“闪存”来存储数据，主要有RS－232串口和并型口的CF(Compact Flash)卡两种形式。但是两种接口都有一定的缺陷：CF卡不能和PC机直接通信，必须通过转接口才能将数据传送到PC机上；而RS-232接口虽然可以和PC机直接通信，但是由于其传输速度较慢，不利于大量数据的传输。由于上述两种接口的缺点，“海马”采用U盘来实现数据的存储。 USB接口不仅可以和PC机直接通信，而且具有使用方便，数据传输率高，又支持即插即用等特点，但由于USB接口协议复杂，涉及的方面广，特别是软件种类比较多，而且USB芯片种类繁多，使得USB设备的开发非常困难。 本文设计的USB(Universal Serial Bus)接口电路以单片机AT89C51以及CYPRESS公司生产的USB接口芯片SL811HS为核心，结合了随机存储芯片HY62256以及看门狗芯片X25165。在介绍了SL811HS控制器芯片的主要特点的基础上，重点阐述了利用这一芯片在C语言的编程控制之下如何识别U盘的插入和拔出，以及如何将单片机的数据按协议规定写入U盘，其中的协议包括FAT文件协议，USB协议和UFI命令协议。 本系统实现了单片机对U盘的数据存储，速度快，存储容量大，数据读取方便，直观明了，系统工作安全可靠，抗干扰能力强，可扩展性大，而且针对下位机设计的串口协议简单，可以满足“海马”海量数据的存储要求。

Phenotypic plasticity of gut structure and function during periods of inactivity in Apostichopus japonicus

Apostichopus japonicus is a common sea cucumber that undergoes seasonal inactivity phases and ceases feeding during the summer months. We used this sea cucumber species as a model in which to examine phenotypic plasticity of the digestive tract in response to food deprivation. We measured the body mass, gross gut morphology and digestive enzyme activities of A. japonicus before, during, and after the period of inactivity to examine the effects of food deprivation on the gut structure and function of this animal. Individuals were sampled semi-monthly from June to November (10 sampling intervals over 178 days) across temperature changes of more than 18 degrees C. On 5 September, which represented the peak of inactivity and lack of feeding, A. japonicus decreased its body mass, gut mass and gut length by 50%, 85%, and 70%, respectively, in comparison to values for these parameters preceding the inactive period. The activities of amylase, cellulase and lipase decreased by 77%, 98%, and 35% respectively, in comparison to mean values for these enzymes in June, whereas pepsin activity increased two-fold (luring the inactive phase. Alginase and trypsin activities were variable and did not change significantly across the 178-day experiment. With the exception of amylase and cellulase, all body size indices and digestive enzyme activities recovered and even surpassed the mean values preceding the inactive phase during the latter part of the experiment (October-November). Principal Component Analysis (PCA) utilizing the digestive enzyme activity and body size index data divided the physiological state of this cucumber into four phases: an active stage, prophase of inactivity peak inactivity, and a reversion phase. These phases are all consistent with previously suggested life stages for this species, but our data provide more defined characteristics of each phase. A. japonicus clearly exhibits phenotypic plasticity (or life-cycle staging) of the digestive tract during its annual inactive period. (C) 2008 Elsevier Inc. All rights reserved.

基于MontaVista Linux的开放式机器人控制器研究与实现

本文提出了一种基于MontaVista Linux操作系统的开放式机器人控制器,该系统采用CAN总线作为控制器的系统总线。由于MontaVista Linux操作系统的开放性和良好的移植性,使得系统可以方便扩展、定制不同的应用软件模块和操作系统模块,并且在各种硬件平台间具有良好的移植性。同时由于采用CAN总线作为系统总线,使得系统可灵活配置且可以通过现场总线与其它设备共享数据。

基于CAN总线的机器人示教盒通讯系统的设计

研发了基于CAN总线的新型机器人示教盒系统,采用ARM芯片为核心,并在其上运行了实时操作系统μC/OS-II。设计了一套CAN网络通讯协议,实现了一个示教盒同时示教多台机器人的一对多示教模式,在很大程度上改进和提高了机器人示教盒的性能。

基于DSP的CANopen通讯协议的实现

CANopen是一种开放的应用层协议,其应用可以进一步提高系统的可靠性、通讯效率及灵活性,而且可以使产品具有很好的兼容性。本文采用CANopen通讯协议实现了CAN总线DSP系统与上位机CAN卡之间的通讯,并通过测试实验验证了信息传递的可靠性,保证了全数字网络化伺服驱动系统中对电机控制的准确性和实时性。

基于CAN的工业机器人内部通信总线设计

介绍了一种基于CAN总线的分布式工业机器人控制器的研究开发情况,实现了总线与各模块的接口设计,并制定出相应的机器人控制器内部通信协议。这种机器人控制器布线简单,系统可灵活扩展,有效提高了工业机器人的性能。

基于CAN总线的力传感器与机器人控制器通讯设计

研磨机器人系统中,研磨头与工件的接触力是保证加工精度,进行机器人力控制的一个重要因 素。采用 CAN 通讯可以确保力传感器准确、及时地把力信息传送给控制器进行力控制。本文 给出了6维腕力传感器与机器人控制器通讯的硬件结构,制定了可靠的通讯协议,实现了力信 息的正确读取,为研磨机器人控制系统获得可靠的力信息提供了一种新的解决方案。

基于CAN总线的物流拣选设备的设计

简要介绍了物流的发展现状 ,详细描述了基于CAN总线的物流拣选系统的结构、供电方式和参数设定方法 ,并为系统硬件设计中的电源转换、总线驱动和地址译码等公共电路以及总线控制器、电子标签和指示灯控制器等主要设备提供了设计方案 ,为系统软件设计中的C5 1编译、通信协议、命令类型和程序控制规划等问题给出了相应的解决方法 ,还与进口同类产品的性能和价格进行了比较