RAMSY: Ratio Analysis of Mass Spectrometry to Improve Compound Identification

The complexity of biological samples poses a major challenge for reliable compound identification in mass spectrometry (MS). The presence of interfering compounds that cause additional peaks in the spectrum can make interpretation and assignment difficult. To overcome this issue, new approaches are needed to reduce complexity and simplify spectral interpretation. Recently, focused on unknown metabolite identification, we presented a new approach, RANSY (ratio analysis of nuclear magnetic resonance spectroscopy; Anal. Chem. 2011, 83, 7616-7623), which extracts the signals related to the same metabolite based on peak intensity ratios. On the basis of this concept, we present the ratio analysis of mass spectrometry (RAMSY) method, which facilitates improved compound identification in complex MS spectra. RAMSY works on the principle that, under a given set of experimental conditions, the abundance/intensity ratios between the mass fragments from the same metabolite are relatively constant. Therefore, the quotients of average peak ratios and their standard deviations, generated using a small set of MS spectra from the same ion chromatogram, efficiently allow the statistical recovery of the metabolite peaks and facilitate reliable identification. RAMSY was applied to both gas chromatography/MS and liquid chromatography tandem MS (LC-MS/MS) data to demonstrate its utility. The performance of RAMSY is typically better than the results from correlation methods. RAMSY promises to improve unknown metabolite identification for MS users in metabolomics or other fields.

CT study of the performance of reciprocating and oscillatory motions in flattened root canal areas

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Jogo de papéis e linguagem: diálogos possíveis entre Vigotski e Bakhtin

In this paper we present ongoing research in which we approach the regulatory role of language in role play in children aged six and seven. In research, we seek an understanding of the relationship between language, ownership and complexity of the children's game. The hypothesis guiding the research is that the dialogical relations between children and adults and among children, while playing, contribute to children's learning game. Procedures are being used interpretive ethnographic research that focuses on the human meaning of social relations, waged by the subjects in a particular historical context and its elucidation and exposition by one researcher. This approach allows closer studies of Vygotsky and Bakhtin scholars of language that emphasize the regulatory role of language in human relationships and value the dialogic exchange between researcher and researched; active subjects of the research process. The data were produced by means of observation and interview, in a field diary records, photographs and audio recordings. They are being screened in thematic groups and analyzed using interpretative narration. The participants were two teachers and two classes of the first year of an Elementary school (EMEF) School of Municipal Elementary School Crane, State of Sao Paulo, totaling 45 subjects. Bearing in mind the above assumptions, are presented and analyzed data that allow to anticipate some results.

Prejuízos educacionais: a semiformação intensificada

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

HPLC-DAD method for metabolic fingerprinting of the phenotyping of sugarcane genotypes

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Cogeneration design problem Computational complexity analysis and solution through an expert system

Purpose - The purpose of this paper is twofold: to analyze the computational complexity of the cogeneration design problem; to present an expert system to solve the proposed problem, comparing such an approach with the traditional searching methods available.Design/methodology/approach - The complexity of the cogeneration problem is analyzed through the transformation of the well-known knapsack problem. Both problems are formulated as decision problems and it is proven that the cogeneration problem is np-complete. Thus, several searching approaches, such as population heuristics and dynamic programming, could be used to solve the problem. Alternatively, a knowledge-based approach is proposed by presenting an expert system and its knowledge representation scheme.Findings - The expert system is executed considering two case-studies. First, a cogeneration plant should meet power, steam, chilled water and hot water demands. The expert system presented two different solutions based on high complexity thermodynamic cycles. In the second case-study the plant should meet just power and steam demands. The system presents three different solutions, and one of them was never considered before by our consultant expert.Originality/value - The expert system approach is not a "blind" method, i.e. it generates solutions based on actual engineering knowledge instead of the searching strategies from traditional methods. It means that the system is able to explain its choices, making available the design rationale for each solution. This is the main advantage of the expert system approach over the traditional search methods. On the other hand, the expert system quite likely does not provide an actual optimal solution. All it can provide is one or more acceptable solutions.

Diversity and Localization of Bacterial Endosymbionts from Whitefly Species Collected in Brazil

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Collaboration of ultrasonography in the diagnosis of thyroid gland carcinoma in a dog - case report

The sonographic evaluation of thyroid glands in veterinary medicine presents challenges due to the complexity of the anatomical region, professional experience and type of ultrasonography equipment. The technique is considered a versatile diagnostic method that is noninvasive and has a low cost indicated in different clinical situations. Thyroid carcinoma is a malignant tumor that is often invasive and frequently metastatic to regional lymph nodes and lungs. The prognostic indicators for survival after surgery include tumor size, histological type, mobility and presence or absence of metastasis. The objective of the present report is to demonstrate the importance of ultrasound as a complementary method in the evaluation of thyroid carcinoma in dogs. At the Dr. Halim Atique Veterinary Hospital, an eight-year-old male Pit Bull was examined due to a history of firm painless swelling, approximately six inches in diameter, in the ventral cervical region, for about two months. The sonogram showed a nodular area, with defined and regular margins, and heterogeneous hypoechoic parenchyma, with areas of cavitation and swelling of the thyroid. Histopathology of the nodule was consistent with carcinoma. After thyreoidectomy and hormone replacemet, the patient is in good clinical condition.

Reclamações e gestão de crise sob a ótica das relações públicas: um estudo da empresa Wet'n Wild São Paulo

For the last few decades the relationship between companies and their market has been changing, driven by economic and political changes, new social demand, and by the technologic evolution. This new setting, combined with the creation of laws and regulatory agencies, increased the complexity of the relationship between companies and their customers, creating the need for strategies capable of handling the contemporary market's relationship. This context creates the need for new actions to deal with post-sale costume care to create customer loyalty and evaluate its overall satisfaction, in addition to treating possible complaints about their products and services. Along with the new customer's requirements in a dynamic communicational scenario, this paper addresses public relations' role in managing customer's complaints in light of crisis management. Based on the concepts of public relations, the approach of crisis and complaints management processes is constructed based on literature review and analysis of Wet'n Wild São Paulo's claims management. Therefore, the method used was document and content analysis based on documents provided by the company in order to evaluate their actions based on the concepts covered

Fragmento d'alma

Fragmento D' Alma is an artistic research that part of a poetic tale through an initial charge of dawn one fantastic universe where words have life and willingly. The work includes sculptural works in order to interpret through the use of allegory situations of the tale in the form of sculptural compositions considering one or more characters in the story. In the sculptural work, I tried figuring the relations tale as my interpretations and poetic, so I sought theoretical studies of allegory after noting my affinity with the theme to contribute to the relationship text and image set in my work. I also sought to understand the relationships that the text played with sculptural work, so the chances that I could address this through dialogue between text and image. The studies collected through surveys pointed allegory by João Adolfo Hansen, as well as in various artistic and visual references present in my repertoire, such as the Baroque sculpture of Bernini, the performances of Berna Reale and literature of Neil Gaiman, contributed in this research to the development of my creative process during the preparation of this research work of art, making the complexity of the relationship between textual production and poetic sculptural three-dimensional, also became a concern and an integral theme of my work and my reflections presented here. Therefore, I conclude based on my own experience in this work, the artistic research inspired allegory might contribute to the development of my creative process during the preparation of this artwork, besides assigning relations between textual production and poetic dimensional sculpture

The Role of the (In)visible and the Secret in the Translation of Harryette Mullen s Muse & Drudge

This paper, based on Jacques Derrida’s thoughts in Des Tours of Babel, addresses the issue regarding the (in)visible in translation, by arguing that the latter, beyond the traditional conception of communication, produces a complex set of relations between the visible and the invisible, which highlights the values of the non-dit and the secret that take place in their relation to interpretation. This line of thought underpins the discussion of my translation of two poems from Muse & Drudge (1995), by the African-American poet Harryette Mullen, whose dense poetry displays un(expected) possibilities of meanings and associations that proliferate in translation. It is argued that every act of translation entails a relationship between that which is translated (and made visible or intelligible through this act) and that which remains invisible and secret by resisting a definitive translation, which, as such, requires further interpretations in search for intelligibility (or “visibility”). We analyze the extent to which such relation between the visible and the invisible takes part in the translation of the notion of blackness raised by Mullen’s poems and how her translated poetry dialogues with issues of reception in Brazilian culture.

The Mutagenic Potential Caused by the Emissions from Combustion of Crude Glycerin and Diesel Fuel

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Estimation of Submerged Aquatic Vegetation Height and Distribution in Nova Avanhandava Reservoir (São Paulo State, Brazil) Using Bio-Optical Modeling

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Typology of companies networks for competitive assessment: an application in higher education institutions

Taking into account the changes in the market scenario by virtue of globalization, Institutes of Higher Education (IES) as well as other organizations seek their competitive stability. For that reason, it is up to organizations to adopt innovative models of management for their operations aimed at improving results. Company networks consist of a model that is perfect for uniting efforts through cooperation among partners in a given business, which can involve ties of different natures. This paper shows the development and the application of an auxiliary technique to analyze the intensity, nature and importance of internal and external relations in the formation of results for a company network. For such, a multiple case study was conducted at two IES in the State of São Paulo and their networks of partners and employees in order to observe their specificities and organizational strategies. The study demonstrated the existence of specific performance criteria (pillars) for each IES and its network, resulting from its competitive reality. It reveals evidence that the education pillar is strengthened in both cases, and the research pillar is growing, although it is the weakest. The outreach pillar is the most robust in the public IES and the financial sustainability pillar is relevant for the private IES, and it was only detected in this IES.

O Camisa 10 em campo: elementos do processo de formação de um futebolista brasileiro

Pós-graduação em Ciências da Motricidade - IBRC