Detection of Myxobolus rotundus (Myxozoa : Myxosporea) in skin mucus of crucian carp Carassius auratus auratus using a monoclonal antibody

Diagnosis of myxosporean Myxobolus rotundus infection was conducted by examining skin mucus from the infected crucian carp Carassius auratus auratus with a monoclonal antibody, MAb 2D12, raised previously against the parasite. A positive reaction was observed in skin mucus collected from infected fish, and spores and pre-spore stages of the parasite were identified by the MAb 2D12. It was also demonstrated that M. rotundus infection can be successfully detected by a simple method, enzyme-linked immunosorbent assay (ELISA), and that skin mucus collected from infected fish skin had a significantly higher optical density (OD) value than that from uninfected fish.

Molecular systematics of Xenocyprinae (Teleostei : Cyprinidae): Taxonomy, biogeography, and coevolution of a special group restricted in east Asia

We surveyed mitochondrial DNA (mtDNA) sequence variation in the subfamily Xenocyprinae from China and used these data to estimate intraspecific, interspecific, and intergeneric phylogeny and assess biogeographic scenarios underlying the geographic structure of lineages. We sequenced 1140 bp of cytochrome b from 30 individuals of Xenocyprinae and one putative outgroup (Myxocypris asiaticus) and also sequenced 297 bp of ND4L, 1380 bp of ND4, 68 bp of tRNA(His), and 69 bp of tRNA(Ser) from 17 individuals of Xenocyprinae and the outgroup (M. asiaticus). We detected high levels of nucleotide variation among populations, species, and genera. The phylogenetic analysis suggested that Distoechodon hupeinensis might be transferred to the genus Xenocypris, the taxonomic status of the genus Plagiognathops might be preserved, and species of Xenocypris and Plagiognathops form a monophyletic group that is sister to the genus Distoechodon and Pseudobrama. The introgressive hybridization might occur among the populations of X. argentea and X. davidi, causing the two species to not be separated by mtDNA patterns according to their species identification, and the process and direction of hybridization are discussed. The spatial distributions of mtDNA lineages among populations of Xenocypris were compatible with the major geographic region, which indicated that the relationship between Hubei + Hunan and Fujian is closer than that between Hubei + Hunan and Sichuan, From a perspective of parasite investigation, our data suggested that the fauna of Hexamita in Xenocyprinae could be used to infer the phylogeny of their hosts. (C) 2001 Academic Press.

Methods for characterization of packaging parasitics of semiconductor lasers

Two novel methods for analyzing the parasitics of packaging networks are proposed based on the relations between the scattering parameters of a semiconductor laser before and after packaging, and the experiments are designed and performed using our methods. It is found that the analysis results of the two methods are in good agreement with the measurements. Either of the two methods can provide an alternative approach for characterizing the packaging parasitics for semiconductor lasers, and both are convenient due to the developed measurement techniques. (c) 2005 Wiley Periodicals, Inc.

The effect of In content on high-density InxGa1-xAs quantum dots

We have successfully grown self-assembled InxGa1-xAs (x = 0.44, 0.47, 0.50) quantum dots (QDs) with high density (> 10(11)/cm(2)) by MBE. The effect of In content on the high-density QD is investigated by atomic force microscopy (AFM) and photoluminescence (PL) spectra. It is found that sample with In-mole-fraction of 0.5 shows small size fluctuation and high PL intensity. The influence of growth temperature on high-density QD is also investigated in our experiment. (c) 2005 Elsevier B.V. All rights reserved.

基于有序标记的IP包追踪方案

包标记方案是一种针对DoS攻击提出的数据包追踪方案，由于其具有响应时间快、占用资源少的特点，近年来受到了研究者的广泛关注．但由于包标记方案标记过程的随机性，使得受害者进行路径重构时所需收到的数据包数目大大超过了进行重构所必需收到的最小数据包数目，从而导致重构误报率的提高和响应时间的增长．本文提出了一种基于有序标记的IP包追踪方案，该方案通过存储每个目标IP地址的标记状态，对包标记的分片进行有序发送，使得在DoS发生时，受害者重构路径所需收到的标记包的数目大大降低，从而提高了对DoS攻击的响应时间和追踪准确度．该算法的提出进一步提高了包标记方案在实际应用中的可行性．

低轮FOX分组密码的碰撞-积分攻击

FOX是最近推出的系列分组密码，它的设计思想基于可证安全的研究结果，且在各种平台上的性能优良．本文利用碰撞攻击和积分攻击相结合的技术分析FOX的安全性，结果显示碰撞-积分攻击比积分攻击有效，攻击对4轮FOX64的计算复杂度是2^45.4，对5轮FOX64的计算复杂度是2^109.4，对6轮FOX64的计算复杂度是2^173.4，对7轮FOX64的计算复杂度是2^237.4，且攻击所需数据量均为2^9；也就是说4轮FOX64／64、5轮FOX64／128、6轮FOX64／192和7轮FOX64／256对本文攻击是不免疫的．

a real-time path planning approach without the computation of cspace obstacles

An important concept proposed in the early stage of robot path planning field is the shrinking of the robot to a point and meanwhile expanding of the obstacles in the workspace as a set of new obstacles. The resulting grown obstacles are called the Configuration Space (Cspace) obstacles. The find-path problem is then transformed into that of finding a collision free path for a point robot among the Cspace obstacles. However, the research experiences obtained so far have shown that the calculation of the Cspace obstacles is very hard work when the following situations occur: 1. both the robot and obstacles are not polygons and 2. the robot is allowed to rotate. This situation is even worse when the robot and obstacles are three dimensional (3D) objects with various shapes. Obviously a direct path planning approach without the calculation of the Cspace obstacles is strongly needed. This paper presents such a new real-time robot path planning approach which, to the best of our knowledge, is the first one in the robotic community. The fundamental ideas are the utilization of inequality and optimization technique. Simulation results have been presented to show its merits.

历史数据实时压缩方法研究

在开发实时数据库中,对历史数据的存储和管理非常重要,由于大量的数据存储,需要高效的历史数据压缩算法。文章基于均方误差的设计思想,提出了一种新的历史数据压缩算法、给出其详细步骤,并和OSI软件公司开发的旋转门算法进行比较。仿真结果表明,该文提出的新压缩算法具有更高的数据压缩率,降低了测量误差对数据压缩影响。

模型驱动体系综述

模型驱动体系是OMG提出的一种IT系统描述方法,是互操作性标准进一步的发展。文章介绍了模型驱动体系的基本概念、核心基础构造及其模型体系,并介绍了当前的应用现状。

广东典型藻类特点及其能源利用可能性研究

大型海藻的生产力高，生产成本低，是一种理想的能源作物。广东省是我国海洋大省之一，约35万平方公里的海域，海藻物种丰富，为海藻养殖提供了有利的条件。我省是能源消耗大省，大部分依靠外省调入和进口，发展海藻能源技术是缓解能源问题的重要途径之一。目前，海藻作为生物质的利用技术还不成熟。在本论文中，选用我省常见的江蓠、马尾藻和麒麟菜三种的大型的海藻进行热解和发酵实验。 首先，对海藻在不同温度下（400℃～900℃）热解得到的各产物（气体、焦油和残渣）产率和热值以及气体组分进行了分析，研究了各热解产物产率、热值和气体组分随温度的变化规律，分析热解过程中的K、Ca、Na、Mg等元素的析出和迁移规律。用去离子水和稀盐酸对海藻进行洗滤预处理，研究其热解特性，进行热重分析，建立海藻热解的反应动力学模型，并计算海藻的动力学参数。分析结果表明：热解气体中的主要成分为H2、CO、CH4、C2H4、C2H6等，热解气低位热值介于5～15 MJ/m3之间。海藻本身具有灰分含量较高和热值较低的特点，水洗可以有效地脱除部分的碱金属，并可以减少灰分含量，改善海藻的热解特性。 其次，以江蓠和马尾藻为底物进行发酵。结果表明：海藻中含有大量金属阳离子，直接发酵容易溶解到料液中，抑制微生物生长，影响发酵效果。用淡水浸泡以后的海藻能容易进行发酵。江蓠的产气率要高于马尾藻。在发酵温度为35℃，简单破碎，料液浓度为5%的条件下，江蓠TS（总固体）产气率是390.6L/kg 。在55℃，颗粒大小0.6～0.9mm，料液浓度为5%的条件下，马尾藻TS产气率是173.1L/kg。 通过对比海藻热解和发酵过程及结果的比较可以发现，海藻热解产气率低，碱金属容易析出；海藻发酵可以直接利用湿原料，产气率高，但发酵时间较长，需要合适的菌种。在目前没有特殊专有技术的情况下，采用发酵比采用热解实现海藻的能源化利用可能性更大。 最后，对本论文的研究探讨进行总结，并对今后进一步完善该工作提出了建议。

70年来陕西省纸坊沟流域农业生态经济系统耦合态势

"农业生态经济系统耦合"的研究与实践对于实现农业产业与资源相一致,建立持续、高效的农业生态经济系统具有重要的意义。耦合度可以阐明农业经济系统与农业生态系统互动关系,判定农业生态经济系统耦合态势。在分析陕西省纸坊沟流域农业生态经济系统耦合关系的基础上,参照系统科学等理论及相关研究结果建立了农业经济系统与农业生态系统耦合度模型,并计算和分析了该流域70a来的耦合度。结果表明,农业生态经济系统的耦合过程可以划分为4个阶段:Ⅰ.经济系统依赖生态资源进行原始化农业生产阶段;Ⅱ.农业生产掠夺式利用生态资源,生态系统供给能力不断减少阶段;Ⅲ.农业经济系统与生态系统协调化发展阶段;Ⅳ.降低农业发展速度,促使生态系统重建阶段。纸坊沟流域从1938～2008年先后经历了第Ⅰ阶段、第Ⅱ阶段和第Ⅲ阶段。目前处于第Ⅲ阶段,但在"系统发展"过程中已潜伏了越来越大的危机,到2018年系统耦合突破"协调"界限,"相悖态势"将明显表现出来。为此,纸坊沟流域必须调整产业布局,发展草畜产业,进行产业升级,优化产业结构,实现农业生态经济系统协调、持续发展。

马家塔露天煤矿复垦区不同土地利用类型的土壤水分入渗过程研究

煤矿复垦区土壤结构重组,严重改变了其某些土壤物理性质,入渗过程也必然受到影响。因此以马家塔露天煤矿为研究对象,利用圆盘入渗仪研究分析复垦区4种土地利用类型(荒地、草地、灌木、林地)下土壤在3种负压(-15 cm,-3 cm,0 cm)下水分入渗过程及其影响机理,探讨了土地利用类型及复垦方式对煤矿复垦区土壤的水分运动过程的影响,为矿区土壤复垦及植被恢复提供理论基础。