Survey of food network in Ovan Lake

Lake Ovan with about 9 hectares is regarded a semi-shallow lake with an average depth 5.2 meter. It is situated in Ghazvin Province, with a 1910 m high in mountainous regime. A monthly sampling was done at 3 stations studying the physicochemical and biological parameters in water and sediment at the Lake for a year. The temperature annual was measured 12.3°C and other parameters are pH as 8.8, oxygen 10, with total mean phosphate and nitrate as 0.14 & 0.8 mg/lit respectively. The chl.a mean was also measured 1.94 mg/lit. The ratio of N to P was calculated as 1:59, indicating a limiting factor for growth. Considering the trophic statues of the lake taking into account as above parameters, it is a mesotrophic lake with medium trophy. Altogether, 53 phytoplankton species were identified mostly diatoms, green algae and cyanobacteria. Although, 14 zooplankton species were identified with daphnia dominating the group. Macrobenthoses were also surveyed and 11 families were identified comprising mainly by Annelids, Gastropod, Bivalves and Insect Larvae. Other organisms were 2 dominate water plant including Phragmites australis covering at the edge of the lake and potamogeton sp in the inner parts, and also 2 fish species, common carp and Pike Perch. The diversity Shannon—Wiener index was calculated for main taxa groups with all figures lower than 3. Regarding the fish potential production of the lake based on Bramic & Lemke and morphoedophic index, it was calculated to be 20.4 kg/ha fish and a total of about 150 kg for the entire lake.

Estimating Population Trends in American Woodcock (Scolopax Minor) Using Population Reconstruction Models

The American woodcock (Scolopax minor) population index in North America has declined 0.9% a year since 1968 prompting managers to identify priority information and management needs for the species (Sauer et al 2008). Managers identified a need for a population model that better informs on the status of American woodcock populations (Case et al. 2010). Population reconstruction techniques use long-term age-at-harvest data and harvest effort to estimate abundances with error estimates. Four new models were successfully developed using survey data (1999 to 2013). The optimal model estimates sex specific harvest probability for adult females at 0.148 (SE = 0.017) and all other age-sex cohorts at 0.082 (SE = 0.008) for the most current year 2013. The model estimated a yearly survival rate of 0.528 (SE = 0.008). Total abundance ranged from 5,206,000 woodcock in 2007 to 6,075,800 woodcock in 1999. This study represents the first population estimates of woodcock populations.

Trois expositions environnementales, un trouble : perturbations endocrine, du métabolisme monocarboné, et du microbiote intestinal maternel pendant la gestation déclenchent des phénotypes de type autistique chez le rat de type sauvage

Les Troubles du Spectre Autistique (TSA) sont caractérisés par deux principaux symptômes : des difficultés de communication sociale et des comportements stéréotypés et intérêts restreints. Les TSA touchent 5 fois plus les garçons que les filles et une augmentation de la prévalence exponentielle et continue a été observée aux États-Unis ces dernières décennies. Cette augmentation ne peut s’expliquer par les facteurs génétiques à eux seuls qui ne représentent que 5 à 15% des cas de TSA. Il est donc indispensable d’identifier de potentiels facteurs de risque environnementaux des TSA. Le but de ce travail est d’étudier différents facteurs environnementaux potentiellement modifiables dans le développement de phénotypes autistiques dans différents modèles précliniques des TSA. Les objectifs spécifiques sont : (i) caractériser les effets neurocomportementaux provoqués par une exposition périnatale simultanée à 5 perturbateurs endocriniens parmi les plus prévalent dans notre environnement quotidien (DEHP, DBP, DiNP, BDE-47, BDE-99) à de faibles doses pertinentes pour l’exposition humaine, (ii) identifier les effets neurocomportementaux associés à une altération périconceptionnelle du microbiote maternelle (iii) déterminer les effets neurocomportementaux associés à une altération périconceptionnelle du métabolisme monocarboné. Les résultats présentés dans cette thèse démontrent le potentiel de chacun de ces facteurs environnementaux d’altérer le développement cérébral fœtal. Chaque condition expérimentale a provoqué l’apparition de traits autistiques chez les rats, avec des spécificités comportementales pour chaque exposition développementale. Des déficits d’interactions sociales ont été observés dans chaque situation expérimentale, associés soit à de l’anxiété, de l’hyperactivité, des altérations d’intégration sensorimotrice, et/ou des stéréotypies. Cela nous force à considérer les TSA comme une pathologie aux multiples facettes où l’hétérogénéité des tableaux cliniques est représentative de l’hétérogénéité des causes possibles. La multitude des interactions environnementales courantes possibles avec l’épigénome pourrait être à la base de la grande diversité observée dans la sévérité des symptômes et / ou des comorbidités des TSA. Ce travail ouvre des perspectives futures de prévention ciblée des TSA fondées sur de potentielles modifications de l’environnement comme la réduction de l’exposition aux perturbateurs endocriniens, ou des supplémentations en donneurs monocarbonés (e.g. acide folique) et/ou probiotiques.

Extracting DNA from ‘jaws’: high yield and quality from archived tiger shark (Galeocerdo cuvier) skeletal material

Archived specimens are highly valuable sources of DNA for retrospective genetic/genomic analysis. However, often limited effort has been made to evaluate and optimize extraction methods, which may be crucial for downstream applications. Here, we assessed and optimized the usefulness of abundant archived skeletal material from sharks as a source of DNA for temporal genomic studies. Six different methods for DNA extraction, encompassing two different commercial kits and three different protocols, were applied to material, so-called bio-swarf, from contemporary and archived jaws and vertebrae of tiger sharks (Galeocerdo cuvier). Protocols were compared for DNA yield and quality using a qPCR approach. For jaw swarf, all methods provided relatively high DNA yield and quality, while large differences in yield between protocols were observed for vertebrae. Similar results were obtained from samples of white shark (Carcharodon carcharias). Application of the optimized methods to 38 museum and private angler trophy specimens dating back to 1912 yielded sufficient DNA for downstream genomic analysis for 68% of the samples. No clear relationships between age of samples, DNA quality and quantity were observed, likely reflecting different preparation and storage methods for the trophies. Trial sequencing of DNA capture genomic libraries using 20 000 baits revealed that a significant proportion of captured sequences were derived from tiger sharks. This study demonstrates that archived shark jaws and vertebrae are potential high-yield sources of DNA for genomic-scale analysis. It also highlights that even for similar tissue types, a careful evaluation of extraction protocols can vastly improve DNA yield.

Contributos para a atuação do SEPNA no âmbito da Educação Ambiental

O presente trabalho surge no âmbito do mestrado em Educação Ambiental (EA), da Escola Superior de Educação do Instituto Politécnico de Bragança. Tem como principal objetivo abordar a temática da sensibilização ambiental, mais especificamente aquela que é desenvolvida pelo Serviço de Proteção da Natureza e do Ambiente (SEPNA) da Guarda Nacional Republicana (GNR), do Comando de Bragança, junto da população rural deste concelho. Foram analisadas quatro áreas de intervenção relacionadas com as áreas florestais: resíduos, caça furtiva e desportiva, pesca furtiva e desportiva e incêndios. O trabalho tem como base uma análise das respostas aos inquéritos realizados junto da população, por forma a verificar a opinião e a perceção da população face à funcionalidade das operações do SEPNA, se o seu modus operandi deve ser mais interventivo na sensibilização para os problemas ambientais, para pôr em prática os princípios do desenvolvimento sustentável, dando importância aos comportamentos de risco que, na maioria dos casos, levam ao desequilíbrio dos ecossistemas, seja pela caça e pesca furtivas, ou pelos incêndios que, na maioria, advêm de comportamentos criminosos. Nesse sentido foi realizado um levantamento de informações relacionadas com as áreas de atuação do SEPNA, nomeadamente o número de coimas nos últimos 4 anos, dentro de cada área de intervenção, assim como as metodologias e recursos usados para a sensibilização ambiental. A análise dos resultados poderá ajudar o SEPNA na planificação das suas ações no futuro, no sentido de tornarem mais eficazes no terreno com vista a uma defesa mais efetiva do meio ambiente e sensibilizando as populações para a adoção de comportamentos a favor do ambiente.

As rodovias e as aves de rapina nocturnas relação entre a densidade, o habitat e a mortalidade

As estradas e tráfego inerente surgem como a criação antrópica mais conspícua e penetrante na paisagem natural, sendo considerados os principais agentes causadores de fragmentação e destruição de habitats, assim como representam um obstáculo físico sem precedentes, limitando as relações directas entre os indivíduos, por diminuição da frequência de dispersão e aumento da mortalidade por atropelamento, impedindo o fluxo natural de genes e suscitando o aumento de fenómenos de inbreeding e perda de heterozigotia. Todos os impactes deletérios associados às rodovias são claramente perceptíveis em vertebrados, onde as aves de rapinas nocturnas não são excepção. Uma vez que estas rapaces beneficiam das suas bermas e orlas, como locais de poiso, nidificação ou como corredores de dispersão através da paisagem, são frequentemente vítimas de mortalidade por atropelamento em estradas, sendo esta problemática considerada actualmente uma das mais recentes e importantes formas de mortalidade não natural em rapinas nocturnas e vinculada como um dos maiores problemas de conservação que afecta este grupo. Não obstante, esse mútuo efeito de atracção/repulsa das estradas a estas rapaces, as rodovias criam uma barreira específica que limita a dinâmica, comportamento e densidade populacional das espécies residentes, reconhecendo-se que o isolamento daí resultante, pode comprometer a viabilidade populacional a longo prazo, podendo mesmo conduzir a altos riscos de extinção das populações locais devido a efeitos estocásticos. Mediante esta problemática, este trabalho debruçou-se sobre um único objectivo principal: a avaliação do impacte das rodovias e do tráfego, na densidade das aves de rapina nocturnas. Este estudo foi efectuado na região Alentejana, abrangendo uma área de cariz tipicamente mediterrânico, delimitada pelas localidades de Montemor-o-Novo, Arraiolos e Évora, sendo seccionada por 143 quilómetros de estradas, divididas em autoestrada, rodovias com elevada e reduzida densidade de tráfego. A monitorização das rapinas nocturnas foi conduzida em dois anos amostrais (2005 e 2007), tendo sido focalizada sobretudo em duas espécies de Strigiformes, a Coruja­ do-mato Strix aluco e o Mocho-galego Athene noctua, recorrendo ao uso de playbacks com reprodução de vocalizações de indivíduos conspecíficos. Foram usadas 32 variáveis explicativas integradas em três grandes grupos: variáveis de estrada, métricas da paisagem, uso do solo, tendo sido analiticamente testadas, recorrendo à aplicação de Modelos Lineares Generalizados. Os principais resultados obtidos demonstram que as variáveis de estrada, aliadas à densidade de tráfego e ruído inerente à sua circulação, são provavelmente, responsáveis por um comportamento de repulsa das espécies de aves de rapina nocturnas em estudo, apresentando estas densidades mais elevadas longe de áreas antropicamente perturbadas e, portanto, de menor qualidade que se encontram adjacentes às rodovias. Todavia a presença de habitat favorável a estas rapaces é provavelmente o descritor com maior poder estatístico no que concerne à sua distribuição e densidade, sendo os montados densos e a presença de zonas agrícolas de sequeiro, positivamente correlacionadas, respectivamente com a densidade de casais reprodutores de Coruja-do-mato e Mocho-galego. Mediante a observação dos resultados será veemente a aplicação de medidas de mitigação específicas, que fundamentalmente considerem o afastamento dos efectivos populacionais longe das estradas e tráfego, conservando e assegurando as características estruturais, requisitos e qualidade dos habitats, de modo a incrementar e garantir a viabilidade e densidade das populações, fidelizando a territorialidade e permanência destas aves nestas áreas. Adicionalmente deverá investir-se na conectividade das manchas de paisagem fragmentada pelas rodovias, criando opções de conservação estratégicas, em zonas ecologicamente mais sensíveis, que não somente minimizem o efeito de repulsa reconhecido nestas aves, mas também os níveis de mortalidade por atropelamento, tornando os ecossistemas mais funcionais para a sobrevivência destes rapaces. ABSTRACT; Roads and traffic are the most conspicuous and pervasive human creation, being the great responsible for fragmentation and habitats destruction, reducing animal movement through landscape, which implies decrease of gene flow and loss of variability that can fragmented populations, thus reducing their sizes and densities. All deleterious impacts associated with roads are clearly visible in vertebrates, where owls aren't exception, being frequent victims of road mortality, since they can use roadside habitats and edges for hunting, nesting or dispersal corridors through the landscape, being nowadays one of the most recent and important causes of non­natural mortality in owls and has been recognized as one of the largest conservation problems affecting this group. However, the attractive and avoidance effect of roads and his edges on owls creates a barrier effect that limits dynamics, behaviour and breeding density of resident species, recognizing that possible isolation, could compromise populations survival, make them more vulnerable to high risks of local extinction due to stochastic effects. Despite that, several authors suggest that owls use roads to hunt, as marginal habitats, or for navigation corridors through the landscape ln this context, the major aims of this study was to verify if there are negative effects of roads on the density of owls, considering traffic as an influencing factor. This study was conducted in Alentejo, covering a typical Mediterranean area, including three main localities Montemor-o-Novo, Évora and Arraiolos, being sectioned by 143 km of roads, including highway, roads with high traffic density, and the remaining with low traffic density. The owl census was conducted in two sampling years (2005 and 2007) and was focused mainly on Little owl Athene noctua and Tawny Owl Strix aluco species, using the playback technique, with conspecific calls. We used 32 explanatory variables, mainly included in three groups: road variables, landscape metrics and land use, having been analytically tested, with application of Generalised Linear Models. The main results show that noisy roads with high traffic density are probably the most responsible for the avoidance behaviour of owls, under the study area, showing density depression near high anthropogenic disturbed areas adjacent to roads. However, the presence of habitat quality to these birds is probably the descriptor with greater statistical power, considering its distribution and density, with the dense oak woodland and croplands and arable lands, positively correlated, respectively with Tawny owl and Little owl density. ln consequence, the great conservation effort should be done in order to keep breeding populations away from roads and traffic, ensuring the structural features, requirements and quality of its habitats in order to enhance and ensure the viability and density of owl's populations in these areas. ln addition, it is important to invest in connectivity between roadside fragmented patches, creating strategic conservation options, in sensitive areas, which minimize the avoidance effect, recognized in owls, but also road-kill levels, making ecosystems more functional to survival of these top predators.

Enhancing Library Instruction with Crossword Puzzles in Kinesiology Classes

The poster was presented at the 2016 Tri-Chapter Meeting (MACMLA, NY-NJ and PHIL Chapters), The 3Ls - Librarians, Leadership and Learning on September 25, 2016 in Philadelphia, PA (http://macmla.libguides.com/tri-chapter2016-posters).

Caza y cazadores en la Castilla bajomedieval

La caza puede considerarse como uno de los ejercicios donde el instinto de agresividad y el espíritu lúdico se encuentran estrechamente ligados. El hombre la practica también para defenderse de una naturaleza hostil, asegurarse el alimento u obtener un beneficio en el mercado; suministradora de carne y de pieles, desempeña un papel innegable en las economías medievales. Aparece reflejada en la Literatura y en el Arte. En las clases superiores de la sociedad se ritualiza. Sus prácticas y sus técnicas son codificadas y enseñadas en libros que le consagran importantes personajes. La caza, en fin, da lugar también a una reglamentación y a un derecho que tienden a concebirla como un monopolio de los poderosos. Este trabajo pasa revista a esos y a otros problemas refiriéndolos al reino de Castilla durante la Baja Edad Media.

Faunal remains from an Almohad (Ad XII/XIII) silo at the Castle of Aljezur (Portugal)

The analysis of a faunal collection from a storage silo found in the castle of Aljezur, dating from the Almohad period (XII/XIIIth centuries AD), is presented. It appears that the community occupying the castle concentrated on hunting species such as the rabbit, wild boar, red deer and Iberian lynx, while evidences of stockbreeding were scarce and centered upon caprines with horses and chicken playing minor role. The presence of a large dog has been assumed to be an aid for hunting but possibly also in herd keeping. With the exception of the lynx, all large mammals evidenced traces of consumption. The domestic cat is taken to represent a pet whereas the lynx had probably a role as a fur provider. It should be noted that equids and the pond turtle were probably food items. Rodents are taken to represent commensals whereas the toad of the Genus Bufo probably represented an intrusive element.

Análise de selecção de áreas de caça por uma população de francelho (Faco naumanni) na planície de Évora

Em Portugal, apesar de haver registos históricos da reprodução desta espécie um pouco por todo o país, o francelho (Falco naumanni, Fleischer 1818) desapareceu de quase todo o território nacional estando actualmente restrito à região do Alentejo. Em 2005, 80% da população encontrava-se nas ZPE´s de Castro Verde e Vale do Guadiana (62% e 18% da população nacional, respectivamente (Catry et al. 2005). Ao longo dos últimos anos têm vindo a ser desenvolvidos trabalhos de investigação com o objectivo de conhecer as necessidades ecológicas da espécie nas diferentes colónias, de forma a tentar conciliar a prática de uma agricultura economicamente viável com a conservação de uma espécie que está dependente das práticas agrícolas tradicionais. Os conflitos entre conservação e alteração das práticas agrícolas levam a desafios na gestão deste tipo de habitats. A população de francelho da ZPE Évora, após um período de 12 anos de ausência da espécie como reprodutora na região, tem vindo a aumentar em número de casais reprodutores, desde 1995. A distribuição da espécie na ZPE de Évora, a sua evolução e os principais factores que afectam a sua reprodução foram por nós estudados no ano de 2007. Foi ainda analisada a disponibilidade de habitat de caça para o francelho na área que envolve a principal colónia de Évora de modo a poderem ser sugeridas medidas de gestão adequadas à sua conservação. Concluiu-se que a manutenção de áreas de caça e a conservação dos locais de nidificação existentes e a criação de novos locais passa inevitavelmente pela sensibilização e informação dos proprietários sobre as medidas a aplicar. O recurso a instrumentos de financiamento neste sentido será primordial; ABSTRACT: In Portugal, although there are historical records of the reproduction of this species all over the country, the lesser kestrel (Falco naumanni) disappeared from almost all the national territory being currently restricted to the Alentejo region. In 2005, 80% of the population was in Castro Verde and Vale do Guadiana SPA (62% and 18% of the national population, respectively) (Catry et al. 2005). Over the past few years have been developed research in order to meet the ecological needs of the species in the different colonies, trying to reconcile the practice of an economically viable agriculture and the conservation of a species that is dependent on traditional farming practices. The conflicts between conservation and changing farming practices lead to challenges in managing this type of habitat. The lesser kestrel population of the Évora SPA, after a 12 year absence of the species as breeding in the region, has being increasing in the number of breeding pairs, since 1995. The distribution of the species in the SPA of Évora, its evolution and the main factors affecting their reproduction were studied in 2007. It was also analyzed the availability of hunting habitat for the kestrel in the area surrounding the main colony of Évora so that appropriate management measures can be suggested to their conservation. It was concluded that maintaining hunting areas and conservation of existing nesting sites and the creation of new local inevitably passes through the awareness and information of the owners on the measures to be implemented. The use of financial instruments and the in this direction will be paramount.

BALD EAGLES (HALIAEETUS LEUCOCEPHALUS) AS INDICATORS OF GREAT LAKES ECOSYSTEM HEALTH

Environmental indicators have been proposed as a means to assess ecological integrity, monitoring both chemical and biological stressors. In this study, we used nestling bald eagles as indicators to quantify direct or indirect tertiary-level contaminant exposure. The spatial and temporal trends of polychlorinated biphenyl (PCB) congeners were evaluated in nestling plasma from 1999–2014. Two hexa-chlorinated congeners, PCB-138 and 153, were detected with the highest frequency and greatest concentrations throughout Michigan. Less-chlorinated congeners such as PCB-52 and 66 however, comprised a greater percentage of total PCB concentrations in nestlings proximate to urbanized areas, such as along the shorelines of Lake Erie. Toxic equivalents were greatest in the samples collected from nestlings located on Lake Erie, followed by the other Great Lakes spatial regions. Nestling plasma samples were also used to measure concentrations of the most heavily-used group of flame retardants, brominated diphenyl ethers (BDEs), and three groups of alternative flame retardants, non-BDE Brominated Flame Retardants (NBFRS), Dechloranes, and organophosphate esters (OPs). BDE-47, 99 and 100 contributed the greatest to total BDE concentrations. Concentrations of structurally similar NBFRs found in this study and recent atmospheric studies indicate that they are largely used as replacements to previously used BDE mixtures. A variety of Dechloranes, or derivatives of Mirex and Dechlorane Plus, were measured. Although, measured at lesser concentrations, environmental behavior of these compounds may be similar to mirex and warrant future research in aquatic species. Concentrations of OPs in nestling plasma were two to three orders of magnitude greater than all other groups of flame retardants. In addition to chemical indicators, bald eagles have also been proposed as indicators to identify ecological stressors using population measures that are tied to the fitness of individuals and populations. Using mortality as a population vitality rate, vehicle collisions were found to be the main source of mortality with a greater incidence for females during white-tailed deer (Odocoileus virginianus) hunting months and spring snow-melt. Lead poisoning was the second greatest source of mortality, with sources likely due to unretrieved hunter-killed, white-tailed deer carcasses, and possibly exacerbated by density-dependent effects due to the growing population in Michigan.

International flavours

It is an Olympic year and we have just witnessed the fantastic games hosted by Rio de Janeiro. Well done to team USA for winning the most medals overall but also well done to so many other nations and individuals who performed so well or were ambassadors in other ways. Teenage swimmer Yusra Mardini who swam for the refugee team and South Africa's Wayde van Niekerk who broke the longstanding 400 m record of Michael Johnson that has stood since 1999. Of course, we must mention sprinter Usain Bolt and swimmer Michael Phelps, who have now transcended superstar status and entered a new level of icon. My personal highlight was the sportsmanship witnessed in the 5000 m when American Abbey D’Agostino was accidentally felled by New Zealand runner Nikki Hamblin. D’Agostino helped Hamblin back to her feet but slumped to the track after realising her own injury. Hamblin helped her up and stayed with her so that both completed the race. The International Olympic Committee has awarded both with the prestigious Pierre de Coubertin award, also known as the International Fair Play Trophy. Fair play is of paramount importance in publishing in peer-reviewed papers. At CLAE we try and maintain, as do other journals, this by ensuring double blind peer review and allowing authors to select the most appropriate handling editor for their submission. Our handling editors are placed across the world (2 in Europe, 1 in the Americas, 1 in Australia and 1 in Asia) and part of their role is to encourage submissions from their region. Over the last decade we certainly have seen more and more papers from places that haven’t previously published in CLAE. In this issue of CLAE we have a true international blend of papers. We have papers from authors from the UK, USA, Iran, Jordan, France, Poland, Turkey, Nigeria, France, Spain and Brazil. I think it's a testament to the continued success of the journal that we are attracting new writers from so many parts of the world and retain papers from more established authors and research centres. We do continue to attract many weaker papers that are rejected early in the review process. Often these will be unexceptional case reports or papers describing a surgical technique. Case reports are published but only those that offer something original and especially those with interesting photographs. In this issue you will see Professor James Wolffsohn (UK) has an interesting paper around a lot of the focus of his recent research activity into clinical evaluation of methods of correcting presbyopia. In this paper he highlights predictors to aid success of presbyopic contact lenses. If you have been involved in any clinical work or research in the field of dry eye disease then you will know well the CLDEQ (Contact Lens Dry Eye Questionnaire) devised by Robin Chalmers and her colleagues (USA). This issue of CLAE details the latest research using the CLDEQ-8 (the 8 item version of the CLDEQ). The Shahroud Eye Cohort Study has produced many papers already and in this issue we see Fotouhi Akbar (Iran) looking at changes in central and peripheral corneal thickness over a five year period. These days we use a lot of new instrumentation, such as optical low-coherence reflectometry. In this issue Emre Güler (Turkey) compares that to a new optical biometry unit. Dry eye is more common and in this issue we see a study by Oluyemi Fasina (Nigeria) to investigate the disease in adults in South-West Nigeria. The TearLab™ is now commonly used to investigate osmolarity and Dorota Szczesna-Iskander (Poland) looks at measurement variability of this device. Following the theme of dry eyes and tear testing Renaud Laballe (France) looks at the use of scleral lenses as a reservoir-based ocular therapeutic system. In this issue we have a couple of papers looking at different aspects of keratoconus. Magdalena Popiela (UK) looks at demographics of older keratoconic patients in Wales, Faik Orucoglu (Turkey) reports a novel scoring system for distinguishing keratoconus from normal eyes, Gonzalo Carracedo (Spain) reports the effect of rigid gas permeable lens wear on dry eye in keratoconus and Hatice Nur Colak (Turkey) compares topographic and aberrations in keratoconus. Other interesting papers you will find are Mera Haddad (Jordan) investigates contact lens prescribing in Jordan, Camilla Fraga Amaral (Brazil) offers a report on the use of ocular prosthetics, Naveed Ahmed Khan (Malaysia) reports of the use of dimethyl sulfoxide in contact lens disinfectant and Michael Killpartrick (UK) offers a short piece with some useful advice on contamination risk factors that may occur from the posterior surface of disposable lenses. So for this issue I would say that the Gold Medal for biggest contribution in terms of papers has to go to Turkey. I could have awarded it to the UK too, but Turkey has three full papers and the UK has two plus one short communication. Turkey is also one of the countries that has shown the largest increase in submissions over the last decade. Finally, welcome aboard to our newest Editorial Board Member Nicole Carnt from Australia. Nicole has been an active researcher for many years and acted as a reviewer for CLAE many times in the past. We look forward to working with you.

A Bayesian approach to study the risk variables for tuberculosis occurrence in domestic and wild ungulates in South Central Spain

BACKGROUND Bovine tuberculosis (bTB) is a chronic infectious disease mainly caused by Mycobacterium bovis. Although eradication is a priority for the European authorities, bTB remains active or even increasing in many countries, causing significant economic losses. The integral consideration of epidemiological factors is crucial to more cost-effectively allocate control measures. The aim of this study was to identify the nature and extent of the association between TB distribution and a list of potential risk factors regarding cattle, wild ungulates and environmental aspects in Ciudad Real, a Spanish province with one of the highest TB herd prevalences. RESULTS We used a Bayesian mixed effects multivariable logistic regression model to predict TB occurrence in either domestic or wild mammals per municipality in 2007 by using information from the previous year. The municipal TB distribution and endemicity was clustered in the western part of the region and clearly overlapped with the explanatory variables identified in the final model: (1) incident cattle farms, (2) number of years of veterinary inspection of big game hunting events, (3) prevalence in wild boar, (4) number of sampled cattle, (5) persistent bTB-infected cattle farms, (6) prevalence in red deer, (7) proportion of beef farms, and (8) farms devoted to bullfighting cattle. CONCLUSIONS The combination of these eight variables in the final model highlights the importance of the persistence of the infection in the hosts, surveillance efforts and some cattle management choices in the circulation of M. bovis in the region. The spatial distribution of these variables, together with particular Mediterranean features that favour the wildlife-livestock interface may explain the M. bovis persistence in this region. Sanitary authorities should allocate efforts towards specific areas and epidemiological situations where the wildlife-livestock interface seems to critically hamper the definitive bTB eradication success.

Evidence of shared bovine viral diarrhea infections between red deer and extensively raised cattle in south-central Spain

Bovine viral diarrhea virus (BVDV) is a pestivirus that affects cattle production worldwide and that can infect other ungulates such as cervids and even wild boar (Sus scrofa). It is believed that domestic livestock can become infected through contact with wild animals, though it is known that infection can spread among wild animals in the absence of contact with livestock. Little is known about the sharing of BVDV infection between wild and domestic animals in the same habitat, which is important for designing eradication campaigns and preventing outbreaks, especially on hunting estates with high animal densities.

Porcine hokovirus in wild boar in Portugal.

Porcine hokovirus (PHoV), also referred to as porcine parvovirus 4 (P-PARV4), a recently discovered parvovirus of swine that is closely related to human parvovirus 4/5 (H-PARV4/5), was first described in Hong Kong. To evaluate the occurrence of P-PARV4 in Portuguese wild boars in the hunting season of 2011/2012,liver and serum samples were tested. P-PARV4 was detected in 24 % of the wild boars analyzed. Phylogenetic analysis showed a close relationship between the P-PARV4 isolates and other P-PARV4 reference strains. This virus appears to be emerging, with yet unknown implications for public health.