Nouvelles donn��es sur le Mioc��ne moyen marin ("Sallomacien") de Gironde (Bassin d'Aquitaine-France) Approche des pal��oenvironnements New data about the maine Middle Miocene ("Sallomacian") of Gironde (Aquitaine Basin); paleoecological outline

The Mid Miocene marine formations of Salles area (former "Sallomacian" stage) have been studied again from numerous outcrops and cores. The deep structural framework influences notably of the characteristics and distribution of the deposits, which are neritic. The stratigraphy is stated precisely thanks to the planktonic fauna and floradetailed examination (probably Serravallian zones NN6 - N12). Several paleobiofacies are reconstituted from the rich invertebrate faunas, which give also paleoclimatic data.

Des+constru����o s��nior, sentimento e teatro : trabalho de projeto

Trabalho de Projecto submetido �� Escola Superior de Teatro e Cinema para cumprimento dos requisitos necess��rios �� obten����o do grau de Mestre em Teatro - especializa����o em Teatro e Comunidade.

La biochronologie des Mammif��res n��og��nes d'Europe. L'��chelle MN, son application �� la succession des faunes du Portugal

Similarly to other organisms, Vertebrates changed during the Cenozoic Era. Mammals are the only ones to change quickly; their well mineralised bones and teeth are often fossilised. They are highly diverse and even isolated teeth can be identified. They are thus a good tool for establishing a biochronological framewoork. Among Mammals, Rodents with a short lifetime evolve more quickly than the large Mammals. In Europe, the first elaborated zonation was investigated by the Regional Committee on Neogene Stratigraphy and issued as the MN Zonation (Mammals Neogene) by Mein, 1976. During the following years, progress in knowledge lead to new charts. The latest one resulted from collective work (de Bruijn et al., 1992). B��ochronology gives relative data; if we desire to have numerical age estimates, we must correlate these results with radiometrie data, marine biostratigraph��cal units or the Geomagnetic Polarity Time Scale. For Europe, these results are summarised by Steininger et al. (1989-1996) and Steininger (1999). After some recents developments on MN Zonation, here is discussed the succession of Neogene small mammals Portuguese localities. Fortunately these localities are in majority inbedded in marine context. Their assigment to MN Zones is proposed and correlations with the spanish Aragonian Scale (Daams el al., 1999) are also suggested. In fact, some differences appear between Portugal and Aragonian assemblages, probably for ecological reasons. Therefore, the MN zonation is always useful for short and long distance biochronological correlations.

Biostratigraphie et pal��o��cologie des peuplements d'ostracodes dans le Dom��rien du Bassin Lusitanien, Portugal

The Domerian sections from the Lusitanian Basin of S��o Pedro de Muel, Raba��al and Tomar have provided us with more than 1100 Ostracods belonging from 18 genus and about 48 species. The faunal diversity and density of the associations decrease in space (from Tomar to Raba��al and S��o Pedro de Muel) and time, with favourable environments for the proliferation of Ostracods at the lower part of the sections (Stokesi subzone) and more hostile at the upper part (Ragazzonii subzone). The Monestieri and Nitescens horizons and the Subnodosus subzone are characterized by a typical assemblage of Ostracods. The palaeoecological Ostracod indexes reveal the fluctuations of the oxygenation, temperature, depth and hydrodynamism of the water, on the different sections and on the whole platform. They display a diachronous cooling in the Lower Domerian series. In the upper part of the Middle and in the Upper Domerian, the deeper, less oxygenated and cooler waters prevent the development of the Ostracod faunas.

Integrated biomarker responses of an estuarine invertebrate to high abiotic stress and decreased metal contamination

An integrated chemical-biological effects monitoring was performed in 2010 and 2012 in two NW Iberian estuaries under different anthropogenic pressure. One is low impacted and the other is contaminated by metals. The aim was to verify the usefulness of a multibiomarker approach, using Carcinus maenas as bioindicator species, to reflect diminishing environmental contamination and improved health status under abiotic variation. Sampling sites were assessed for metal levels in sediments and C. maenas, water abiotic factors and biomarkers (neurotoxicity, energy metabolism, biotransformation, anti-oxidant defences, oxidative damage). High inter-annual and seasonal abiotic variation was observed. Metal levels in sediments and crab tissues were markedly higher in 2010 than in 2012 in the contaminated estuary. Biomarkers indicated differences between the study sites and seasons and an improvement of effects measured in C. maenas from the polluted estuary in 2012. Integrated Biomarker Response (IBR) index depicted sites with higher stress levels whereas Principal Component Analysis (PCA) showed associations between biomarker responses and environmental variables. The multibiomarker approach and integrated assessments proved to be useful to the early diagnosis of remediation measures in impacted sites.

Modification of cell volume and proliferative capacity of Pseudokirchneriella subcapitata cells exposed to metal stress

The impact of metals (Cd, Cr, Cu and Zn) on growth, cell volume and cell division of the freshwateralga Pseudokirchneriella subcapitata exposed over a period of 72 h was investigated. The algal cells wereexposed to three nominal concentrations of each metal: low (closed to 72 h-EC10values), intermediate(closed to 72 h-EC50values) and high (upper than 72 h-EC90values). The exposure to low metal concen-trations resulted in a decrease of cell volume. On the contrary, for the highest metal concentrations anincrease of cell volume was observed; this effect was particularly notorious for Cd and less pronouncedfor Zn. Two behaviours were found when algal cells were exposed to intermediate concentrations ofmetals: Cu(II) and Cr(VI) induced a reduction of cell volume, while Cd(II) and Zn(II) provoked an oppositeeffect. The simultaneous nucleus staining and cell image analysis, allowed distinguishing three phases inP. subcapitata cell cycle: growth of mother cell; cell division, which includes two divisions of the nucleus;and, release of four autospores. The exposure of P. subcapitata cells to the highest metal concentrationsresulted in the arrest of cell growth before the first nucleus division [for Cr(VI) and Cu(II)] or after thesecond nucleus division but before the cytokinesis (release of autospores) when exposed to Cd(II). Thedifferent impact of metals on algal cell volume and cell-cycle progression, suggests that different toxic-ity mechanisms underlie the action of different metals studied. The simultaneous nucleus staining andcell image analysis, used in the present work, can be a useful tool in the analysis of the toxicity of thepollutants, in P. subcapitata, and help in the elucidation of their different modes of action.

Mitochondria are the main source and one of the targets of Pb (lead)-induced oxidative stress in the yeast Saccharomyces cerevisiae

The yeast Saccharomyces cerevisiae is a useful model organism for studying lead (Pb) toxicity. Yeast cells of a laboratory S. cerevisiae strain (WT strain) were incubated with Pb concentrations up to 1,000 ��mol/l for 3 h. Cells exposed to Pb lost proliferation capacity without damage to the cell membrane, and they accumulated intracellular superoxide anion (O2 .���) and hydrogen peroxide (H2O2). The involvement of the mitochondrial electron transport chain (ETC) in the generation of reactive oxygen species (ROS) induced by Pb was evaluated. For this purpose, an isogenic derivative ��0 strain, lacking mitochondrial DNA, was used. The ��0 strain, without respiratory competence, displayed a lower intracellular ROS accumulation and a higher resistance to Pb compared to the WT strain. The kinetic study of ROS generation in yeast cells exposed to Pb showed that the production of O2 .��� precedes the accumulation of H2O2, which is compatible with the leakage of electrons from the mitochondrial ETC. Yeast cells exposed to Pb displayed mutations at the mitochondrial DNA level. This is most likely a consequence of oxidative stress. In conclusion, mitochondria are an important source of Pb-induced ROS and, simultaneously, one of the targets of its toxicity.

Math without stress ��� an open online learning project

MOOC (as an acronym for Massive Open Online Courses) are a quite new model for the delivery of online learning to students. As ���Massive��� and ���Online���, these courses are proposed to be accessible to many more learners than would be possible through conventional teaching. As ���Open��� they are (frequently) free of charge and participation is not limited by the geographical situation of the learners, creating new learning opportunities in Higher Education Institutions (HEI). In this paper we describe a recently started project ���Matem��tica 100 STRESS��� (Math Without STRESS) integrated in the e-IPP project | e-Learning Unit of Porto���s Polytechnic Institute (IPP) which has created its own MOOC platform and launched its first course ��� Probabilities and Combinatorics ��� in early June/2014. In this MOOC development were involved several lecturers from four of the seven IPP schools.

Le moment venu ou l���eveil d��s editoriaux. Mutations de la litt��rature frain��aise des ann��es quatre-vingt vues par les revues litt��raires

Les ann��es quatre-vingt signalent un point de bascule dans et une mutation majeure dans les caract��ristiques narratives de la litt��rature fran��aise. D���une certaine fa��on, elles entament la contemporan��it�� litt��raire telle que nous la connaissons du point de vue critique. Nous insisterons sur le r��le des revues et des ��ditoriaux dans ce processus. Ils manifestent quelques h��sitations de la critique par rapport �� la litt��rature naissante.

Atlas des fossiles caract��ristiques du Lias portugais* III a) Dom��rien (Ammonites)

This fascicle, dedicated to the Domerian (Late Pliensbachian) ammonites faunas, is the third part of the ���Atlas des fossiles caract��ristiques du Lias portugais���. It follows two previous publications about the Hettangian-Sinemurian and the Carixian (Lower fifty species are concisely described and illustrated. The other groups (nautilids, belemnites, brachiopods, bivalvia...) will be published afterwards. The principal objective of the ���Atlas des fossiles caract��ristiques du Lias portugais��� is to make the main liassic lusitanian fossils known to a wide public. It also aims to support field studies by giving a rich iconography dedicated to the lusitanian invertebrate macrofaunas. In the case of the ammonites, all the species are described and the ���Atlas��� can be used as an exhaustive database for biostratigraphic and paleobiogeographic studies.

The portuguese public administration reforms potencial impact in the development of civil servants work related stress : SIADAP case

Poster apresentado na 8.�� Confer��ncia da European Academy of Occupational Health Psychology, em Valencia, 12-14 de novembro de 2008.

Utiliza����o de m��todos ��pticos de correla����o de imagem para a determina����o da tenacidade �� fractura de adesivos estruturais

Mestrado em Engenharia Mec��nica

Impacto da Radioterapia no stress oxidativo em modelos celulares radiorresistentes em contexto de obesidade

Introdu����o: Estudos anteriores em modelos tumorais de glioma e melanoma, tumores radiorresistentes, indicaram que a obesidade pode estar relacionada com um aumento do status oxidativo e com a diminui����o da resist��ncia �� radia����o. Como a Radioterapia �� o tratamento frequentemente utilizado para esta patologia, propomo-nos, desta forma, a explorar a influ��ncia da obesidade em c��lulas de glioma, as BC3H1, e melanoma, B16F10, submetidas a Radioterapia, na presen��a de agentes oxidantes e antioxidantes, para o estudo da sua influ��ncia ao n��vel da viabilidade celular e do impacto do stress oxidativo. M��todos: As c��lulas BC3H1 e B16F10 foram tratadas com t-BOOH (150��M e 50 ��M, respetivamente), TUDCA (25��M e 1��M, respetivamente) e com a mistura de t-BOOH+TUDCA em meio DMEM sem soro e meio condicionado (CM), a partir de adip��citos 3T3-L1. Em seguida, parte das c��lulas foram irradiadas com uma dose total de 2Gy. Posteriormente avaliou-se a viabilidade celular (teste MTT) e o stress oxidativo (teste TBARS, atividade da catalase, concentra����o da GSH, e status antioxidante total), ��s 4h e 12h. Resultados: Observou-se um aumento da capacidade antioxidante total das c��lulas irradiadas, comparativamente com as c��lulas n��o irradiadas. O meio condicionado reduziu o stress oxidativo nas BC3H1, ao mesmo tempo que reduziu a sua viabilidade celular. O TUDCA nas c��lulas incubadas com MC e submetidas a radioterapia, tendencialmente diminuiu a viabilidade celular, nas concerta����es em estudo. Discuss��o/Conclus��o: O meio condicionado e a radioterapia, por si s��, aumentam a resposta antioxidante total na c��lula, ��s 4h e ��s 12h. O TUDCA nas c��lulas incubadas com meio condicionado e submetidas a radioterapia, teve um comportamento citot��xico para as BC3H1, nas concentra����es testadas. Revelando a necessidade de aprofundar os estudos da a����o deste composto como agente radiossensibilizador, neste e noutros modelos celulares de carcinog��nese.

Matem��tica 100 stress: um projeto aberto no IPP

A Era Tecnol��gica em que nos vemos inseridos, cujos avan��os acontecem a uma velocidade vertiginosa exige, por parte das Institui����es de Ensino Superior (IES) uma atitude proactiva no sentido de utiliza����o dos muitos recursos dispon��veis. Por outro lado, os elementos pr��prios da sociedade da informa����o ��� flexibilidade, forma����o ao longo da vida, acessibilidade �� informa����o, mobilidade, entre muito outros ��� atuam como fortes impulsionadores externos para que as IES procurem e analisem novas modalidades formativas. Perante a mobilidade crescente, que se tem revelado massiva, a aprendizagem tende a ser cada vez mais individualizada, visual e pr��tica. A conjuga����o de v��rias formas/tipologias de transmiss��o de conhecimento, de m��todos did��ticos e mesmo de ambientes e situa����es de aprendizagem induzem uma melhor adapta����o do estudante, que poder�� procurar aqueles que melhor v��o ao encontro das suas expetativas, isto ��, favorecem um processo de ensino-aprendizagem eficiente na perspetiva da forma de aprender de cada um. A defini����o de pol��ticas estrat��gicas relacionadas com novas modalidades de ensino/forma����o tem sido uma preocupa����o constante na nossa institui����o, nomeadamente no dom��nio do ensino �� dist��ncia, seja ele e-Learning, b-Learning ou, mais recentemente, ���open-Learning���, onde se inserem os MOOC ��� Massive Open Online Courses (n��o esquecendo a vertente m-Learning), de acordo com as v��rias tend��ncias europeias (OECD, 2007) (Comiss��o Europeia, 2014) e com os objetivos da ���Europa 2020���. Neste sentido surge o Projeto Matem��tica 100 STRESS, integrado no projeto e-IPP | Unidade de e-Learning do Polit��cnico do Porto que criou a sua plataforma MOOC, abrindo em junho de 2014 o seu primeiro curso ��� Probabilidades e Combinat��ria. Pretendemos dar a conhecer este Projeto, e em particular este curso, que envolveu v��rios docentes de diferentes unidades org��nicas do IPP.

Math without stress: an open online learning project

MOOC (as an acronym for Massive Open Online Courses) are a quite new model for the delivery of online learning to students. As ���Massive��� and ���Online���, these courses are proposed to be accessible to many more learners than would be possible through conventional teaching. As ���Open��� they are (frequently) free of charge and participation is not limited by the geographical situation of the learners, creating new learning opportunities in Higher Education Institutions (HEI). In this paper we describe a recently started project ���Matem��tica 100 STRESS��� (Math Without STRESS) integrated in the e-IPP project | e-Learning Unit of Porto���s Polytechnic Institute (IPP) which has created its own MOOC platform and launched its first course ��� Probabilities and Combinatorics ��� in early June/2014. In this MOOC development were involved several lecturers from four of the seven IPP schools.