Transplantes de córneas no estado do Rio Grande do Norte: aspectos epidemiológicos e clínicos

This study aimed to characterize in a clinical and epidemiological way the patients who are on a waiting list for transplantation and the patients transplanted with corneal tissue in a corneal transplants reference service in the state of Rio Grande do Norte. It is an epidemiological study of a quantitative approach, with cross-sectional, descriptive and analytical cut including all patients on the waiting list for transplantation (population A) and the patients already transplanted with a corneal tissue (population B) in a reference service. In population A, there was a census conducted of patients on the waiting list for corneal transplantation (n=62 patients). In population B, the sample was non-probabilistic and corresponded to all corneal transplants performed in the service in the period from 2010 to 2014 (n=258). This study is approved by the Research Ethics Committee of the Federal University of Rio Grande do Norte, in Opinion 876 177 and CAAE 37533014.8.0000.5537. Data were collected in full in the period from January to April 2015, by two instruments built to systematize the necessary data collection. After being coded and tabulated, data were analyzed using the Statistical Package for Social Sciences software, version 20.0. The definition of variables and their distribution patterns were presented as frequencies and measures of central tendency while, for multivariate analysis, the effect of magnitude measures were applied (prevalence ratio) and measures of association (chi-square test or Fisher's exact test) for a 0.05 significance level. The results are shown in two scientific articles coming from the field survey data. It was found that the epidemiological profile of patients on the waiting list (n=62) showed a prevalence of individuals aged over 50 years old, female (54.84%) and residents of the middle region of East Rio Grande do Norte (66.13%). The clinical profile of patients with corneal transplantation (n=258) was characterized by being male (51.16%) with an average age of 49.33 years old and 57.75% were coming from East Rio Grande do Norte. The average time on the waiting list was 172.63 days in elective transplants and 9.03 days in urgent transplants. Keratoconus was the main indicator condition to perform the transplant. For patients on the waiting list, the variable “type of disorder of the cornea” showed statistically significant association with gender and age. For patients with corneal transplants, the variable "type of disorder of the cornea" was associated with the variables gender, age, previous surgery, failure of previous graft, classification of the eye and glaucoma. By characterizing the clinical and epidemiological profile of corneal transplants, it is possible to question the reality, pointing about the care that should be offered and develop targeted interventions to collective and individual needs intrinsic to patients who need this surgery as a treatment option.

Toothache and social and economic conditions among adolescents in Northeastern Brazil

O objetivo deste estudo foi relacionar dor de dente com condição socioeconômica, acesso aos serviços de saúde bucal e estilo de vida em adolescentes do município de Sobral, Ceará, desenvolvido com delineamento transversal analítico, com amostra composta por 688 indivíduos. A prevalência de dor de dente no grupo pesquisado foi de 31,8%. Para avaliar a relação entre as variáveis independentes e a dor de dente, realizou-se teste de associação pelo qui-quadrado, estimando-se a razão de prevalências por meio da regressão de Poisson. Os fatores que mais demonstraram relação com a dor de dente foram severidade da cárie, motivo do atendimento odontológico relacionado com urgência, frequência ao dentista e recebimento de escova na escola. Observou-se que a alta prevalência de dor de dente em adolescentes está diretamente relacionada às condições de acesso, assim como às características das ações desenvolvidas pelos serviços de saúde. Assim como há necessidade da implantação de serviços vinculados à promoção de saúde, pautados pela equidade e integralidade, é necessária a implantação de serviços de urgência que não simplesmente intervenham na dor de forma mutiladora, mas a encarem como mecanismo de estímulo ao desenvolvimento de procedimentos de prevenção das doenças bucais.

Ocean acidification affects the phyto-zoo plankton trophic transfer efficiency

The critical role played by copepods in ocean ecology and biogeochemistry warrants an understanding of how these animals may respond to ocean acidification (OA). Whilst an appreciation of the potential direct effects of OA, due to elevated pCO2, on copepods is improving, little is known about the indirect impacts acting via bottom-up(food quality) effects. We assessed, for the first time, the chronic effects of direct and/or indirect exposures to elevated pCO2 on the behaviour, vital rates, chemical and biochemical stoichiometry of the calanoid copepod Acartia tonsa. Bottom-up effects of elevated pCO2 caused species-specific biochemical changes to the phytoplanktonic feed, which adversely affected copepod population structure and decreased recruitment by 30 %. The direct impact of elevated pCO2 caused gender-specific respiratory responses in A.tonsa adults, stimulating an enhanced respiration rate in males (> 2-fold), and a suppressed respiratory response in females when coupled with indirect elevated pCO2 exposures. Under the combined indirect+direct exposure, carbon trophic transfer efficiency from phytoplankton-to-zooplankton declined to < 50 % of control populations, with a commensurate decrease in recruitment. For the first time an explicit role was demonstrated for biochemical stoichiometry in shaping copepod trophic dynamics. The altered biochemical composition of the CO2-exposed prey affected the biochemical stoichiometry of the copepods, which could have ramifications for production of higher tropic levels, notably fisheries. Our work indicates that the control of phytoplankton and the support of higher trophic levels involving copepods have clear potential to be adversely affected under future OA scenarios.

Migrant domestic workers, vulnerability and the law: immigration and employment laws in Cyprus and Spain

The intersection of gender, welfare and immigration regimes has been one of the main focus of a rich scholarship on paid domestic work in Europe. This article brings into the discussion the nexus of employment and immigration law regimes to reflect on the role of legal regulation in structuring and reducing the vulnerability of domestic workers. I analyse this nexus by looking at the cases of Cyprus and Spain, two states falling under the cluster of Southern Mediterranean welfare regimes, that share certain characteristics in terms of immigration regimes, but have substantially different employment law regulation models. The first part sketches the debate on the employment law regulation of domestic work. The second part starts by giving an overview of the immigration regimes of Cyprus and Spain in relation to migrant domestic workers and then proceeds to analyse the two countries’ models and substance of employment law regulation in domestic work. The comparison of these two divergent approaches informs the debate on how the legal regulation of domestic work should be best structured. In Spain there have been recent dynamic legislative changes in the employment law regulation of domestic work. The final part of the article traces these changes and reflects on why such processes have not taken place in Cyprus.

A utilização de serviços odontológicos entre crianças e fatores associados em Sobral, Ceará, Brasil

NORO, L. R. A. et al. A utilização de serviços odontológicos entre crianças e fatores associados em Sobral, Ceará, Brasil. Cad. Saúde Pública, v. 24, n. 7, p. 1509-1516. 2008. ISSN 0102-311X.

Symptom Clusters and Survival in Portuguese Patients with Advanced Cancer

This study aimed to identify clusters of symptoms, to determine the patient characteristics associated with identified, and determine their strength of association with survival in patients with advanced cancer (ACPs). Consecutively eligible ACPs not receiving cancer-specific treatment, and referred to a Tertiary Palliative Care Clinic, were enrolled in a prospective cohort study. At first consultation, patients rated 9 symptoms through the Edmonton Symptom Assessment System (0-10 scale) and 10 others using a Likert scale (1-5). Principal component analysis was used in an exploratory factor analysis to identify. Of 318 ACPs, 301 met eligibility criteria with a median (range) age of 69 (37-94) years. Three SCs were identified: neuro-psycho-metabolic (NPM) (tiredness, lack of appetite, lack of well-being, dyspnea, depression, and anxiety); gastrointestinal (nausea, vomiting, constipation, hiccups, and dry mouth) and sleep impairment (insomnia and sleep disturbance). Exploratory factor analysis accounted for 40% of variance of observed variables in all SCs. Shorter survival was observed for patients with the NPM cluster (58 vs. 23, P < 0.001), as well as for patients with two or more SCs (45 vs. 21, P = 0.005). In a multivariable model for survival at 30-days, age (HR: 0.98; 95% CI: 0.97-0.99; P = 0.008), hospitalization at inclusion (HR: 2.27; 95% CI: 1.47-3.51; P < 0.001), poorer performance status (HR: 1.90, 95% CI: 1.24-2.89; P = 0.003), and NPM (HR: 1.64; 95% CI: 1.17-2.31; P = 0.005), were associated with worse survival. Three clinically meaningful SC in patients with advanced cancer were identifiable. The NPM cluster and the presence of two or more SCs, had prognostic value in relation to survival.

Estilos Educativos Parentais (EMBU-A), Sintomatologia Depressiva/Ansiosa, Stress e Autoestima, numa Amostra de Adolescentes

Introdução. Os estilos educativos parentais enquanto clima educativo geral apresentam um forte impacto em diferentes resultados desenvolvimentais (e.g., sintomatologia depressiva/ansiosa e auto estima) da criança/jovem. Podem ser estudados de um ponto de vista dimensional, considerando, por exemplo, as dimensões Controlo/Sobreproteção e Suporte, ou categorial, seguindo, por exemplo, a abordagem de Baumrind, e Maccoby e Martin. Neste estudo pretendemos: verificar se existem diferenças por género e idade, nas dimensões Suporte Emocional, Sobreproteção e Rejeição (EMBU-A) (cada progenitor separadamente), na sintomatologia depressiva/ansiosa e na auto estima; avaliar se existem diferenças nas dimensões do EMBU-A, entre progenitores, na amostra total e por género; explorar associações entre as variáveis centrais do estudo (estilos educativos, sintomatologias e auto estima) e variáveis sociodemográficas, também nas três amostras; explorar diferenças entre quatro grupos criados (Pai-Filho, Pai-Filha; Mãe-Filho e Mãe-Filha) nas dimensões do EMBU-A; combinando a Sobreproteção e o Suporte Emocional (EMBU-A), e definindo os estilos educativos parentais autoritário, autoritativo, permissivo e negligente, calcular a prevalência dos mesmos na nossa amostra (por Pai e por Mãe) e explorar as suas associações com as sintomatologias e com a auto estima (separadamente, por progenitor). Metodologia. A nossa amostra é constituída por 284 adolescentes (idade média = 14,5; DP = 1,68; raparigas, n = 171, 60,2%). Todos preencheram um protocolo composto por um questionário sociodemográfico, pela Rosenberg Self- Esteem Scale (RSES), pelo DASS-21 e pelo Parental Rearing Style Questionnaire for use with Adolescents (EMBU-A/A). Resultados. De salientar o facto de os adolescentes percecionarem a mãe como apresentando valores mais elevados em todas as dimensões do EMBU-A, por comparação com o pai. Da mesma forma, ainda que com diferentes padrões de associação conforme o género, é de salientar a associação entre níveis maiores de Suporte emocional e níveis mais baixos de sintomatologia depressiva/ansiosa e stress, e a associação entre as dimensões Sobreproteção e Rejeição e níveis superiores desses resultados. Uma mais baixa escolaridade do pai associa-se a menor Suporte emocional e uma maior escolaridade da mãe a maior Sobreproteção. Uma díade constituída por progenitor e adolescente do género masculino apresenta resultados inferiores em todas as dimensões do EMBU-A, por comparação com díades formadas por mãe-filho/filha. O estilo Autoritativo é o mais prevalente na nossa amostra (em ambos os progenitores) e o estilo Autoritário é aquele que se associa a pontuações mais elevadas de psicopatologia e a uma menor auto estima. Discussão. De uma forma, genérica os resultados seguem de perto a literatura e revelam a associação entre valores mais elevados na dimensão Suporte emocional e níveis mais baixos de psicopatologia e stress, e a associação entre as dimensões Sobreproteção e Rejeição e níveis superiores desses resultados. Igualmente, o estilo educativo Autoritário está claramente associado a piores resultados nessas mesmas variáveis. São discutidas algumas implicações, no que toca a programas psicoeducativos/educação parental. / Introduction. As a general educational environment, parental rearing styles have a strong impact in different outcomes of development of the child or teenager (e.g. symptoms of depression/ anxiety and self-esteem). These can be studied from a dimensional point of view considering for example the dimensions Control/Overprotection and Support or from a categorical point of view following for example the approach of Baumrind and Maccoby and Martin. This study aims at checking whether there are differences of gender and age in the dimensions of Emotional Support, Overprotection and Rejection (EMBU-A) (each parent separately) in depressive symptoms/anxiety and self esteem; assessing whether there are differences in the dimensions of the EMBU-A, between parents in the total sample and by gender; exploring associations between the study’s main variables (rearing styles, symptomatology and self esteem) and sociodemographic variables, also in the three samples; exploring differences between the four groups created (Father-Son, Father-Daughter, Mother-Son and Mother-Daughter) in the dimensions of the EMBU-A, combining Overprotection and Emotional Support(EMBU-A) and defining the authoritarian, authoritative, indulgent, and neglectful parental rearing styles. The aim is also to estimate the prevalence of these in our sample (by Father and Mother) and explore their associations to the symptomatology and self esteem (separately, per parent). Methodology. Our sample is composed of 284 teenagers (average age = 14,5; DP = 1,68; girls, n = 171, 60,2%). All participants filled in a protocol of questionnaires consisting of a set of socio demographic questions by Rosenberg Self- Esteem Scale (RSES), by DASS-21 and by Parental Rearing Style Questionnaire for use with Adolescents (EMBU-A/A). Outcomes. It is important to emphasize the fact that teenagers perceive their mother as having higher values in all the EMBU-A dimensions compared to their father. In the same way, though with different patterns of association according to gender, it is important to emphasize the association of higher levels of Emotional Support and lower levels of depressive/anxious symptomathology and stress and the association of Overprotection and Rejection and higher levels of those outcomes. A father’s lower level of education is associated to a lower Emotional Support while a mother’s higher level of education is associated to a greater Overprotection. A dyad composed of male parent and male teenager presents lower outcomes in all EMBU-A dimensions if compared to dyads composed of mother-son/daughter. The authoritative style is the most prevalent in our sample (in both parents) and the authoritarian style is the one associated to higher scores of psychopathology and lower levels of self esteem. Debate. In general, the outcomes closely follow the literature review and reveal the association of higher values in the Emotional Support dimension and lower levels of psychopathology and stress and also the association of the dimensions of Overprotection and Rejection and higher levels of those outcomes. Equally, the authoritarian rearing style is clearly associated to the worst outcomes in those same variables. Some implications are discussed as far as psychoeducational programmes and parental rearing are concerned.

Rede Social Pessoal, Qualidade de Vida e Depressão em Idosos

Em Portugal e no Mundo Ocidental a população está a envelhecer, colocando esta nova realidade enormes desafios à sociedade. A sua crescente relevância deve-se sobretudo às consideráveis repercussões a nível pessoal, familiar, sociopolítico e económico e que afetam pessoas de todas as idades e a sociedade como um todo, colocando desafios específicos relativamente às relações interpessoais, à qualidade de vida e à saúde mental na pessoa idosa. Deste modo, o presente trabalho tem como objetivo analisar a associação entre a qualidade de vida, a depressão e as características das redes sociais pessoais dos idosos. Participaram no estudo 317 indivíduos, sendo 202 do sexo feminino e 115 do sexo masculino, com idade igual ou superior a 65 anos, com uma média de 77 anos (DP=7,57). Na recolha de dados recorremos a três instrumentos: Geriatric Depression Scale (GDS Short Form 15), (Yesavage et al., 1983; Almeida & Almeida, 1999); Instrumento de Avaliação de Qualidade de Vida da OMS (WHOQOL), (OMS, 1998; Canavarro et al., 2006); Instrumento de Avaliação das Redes Sociais Pessoais (IARSP – Idosos), (Guadalupe, 2010; Guadalupe & Vicente, 2012). Dos resultados destacamos que as características funcionais da rede social pessoal se diferenciaram de forma estatisticamente significativa entre as subamostras de idosos segundo os níveis de qualidade de vida percebida. Saliente-se que, além da relação significativa entre a depressão e qualidade de vida, em que os idosos com um nível mais baixo de qualidade de vida percebida apresentam maiores níveis de depressão (p<0,001), as características funcionais das redes sociais apresentam uma associação clara com a qualidade de vida (p<0,005) e a maioria com a depressão (p<0,014), o que não acontece com as estruturais e com as relacionais-contextuais. Outros resultados indicam que indivíduos com diferentes níveis de qualidade de vida percebida possuem uma estrutura idêntica da rede social pessoal. Ao nível da análise da associação entre as variáveis funcionais da Rede Social Pessoal, Qualidade de Vida e Depressão, o modelo analítico transmite-nos indicadores de investigação e intervenção precisos, o que demonstra a necessidade da continuidade e aprofundamento do presente estudo num âmbito amostral mais alargado e heterogéneo. / In Portugal and in the eastern world, the aging of population creates huge challenges to societies. It's growing relevance is owed to considerable repercussions on the personal, familiar, socio-politic and economic level that affect people of all ages and society as a whole, creating specific challenges regarding interpersonal relationships, quality of life and mental health of the elderly. The current work has the objective of analyzing the association between quality of life, depression and the characteristics of personal social networks of the elderly. 317 individuals have participated in this study, 202 female and 115 male, with age equal or above 65 years old, with an average of 77 years old (DP=7,57). We used three assessment instruments to collect data: Geriatric Depression Scale (GDS Short Form 15), (Yesavage et al., 1983; Almeida & Almeida, 1999); WHO’s Quality of Life Evaluation instrument (WHOQOL) (WHO, 1998; Canavarro et al., 2006); Personal Social Network Analysis Tool (IARSP-Elderly),(Guadalupe, 2010; Guadalupe & Vicente, 2012). The results show that the functional personal network characteristics are significantly different according to their level of quality of life. It should also be noted that not only there is a significant association between depression and quality of life, in which elderly people with a lower quality of life level show higher levels of depression (p<0,001), there is also a clear association between the functional social network characteristics and quality of life (p<0,005), and the majority with depression (p<0,014), which doesn’t happen with structural and relational-contextual social network characteristics. Other results indicate that different levels of quality of life acquire an identical social network structure. On the matter of association between the functional variables of social networks, quality of fife and depression, the analytic model shows precise indicators of research and intervention, which instills us a need to continue and enlarge this study with an wider and more heterogeneous sample.

Trans-ethnic fine mapping highlights kidney-function genes linked to salt sensitivity

We analyzed genome-wide association studies (GWASs), including data from 71,638 individuals from four ancestries, for estimated glomerular filtration rate (eGFR), a measure of kidney function used to define chronic kidney disease (CKD). We identified 20 loci attaining genome-wide-significant evidence of association (p < 5 × 10(-8)) with kidney function and highlighted that allelic effects on eGFR at lead SNPs are homogeneous across ancestries. We leveraged differences in the pattern of linkage disequilibrium between diverse populations to fine-map the 20 loci through construction of "credible sets" of variants driving eGFR association signals. Credible variants at the 20 eGFR loci were enriched for DNase I hypersensitivity sites (DHSs) in human kidney cells. DHS credible variants were expression quantitative trait loci for NFATC1 and RGS14 (at the SLC34A1 locus) in multiple tissues. Loss-of-function mutations in ancestral orthologs of both genes in Drosophila melanogaster were associated with altered sensitivity to salt stress. Renal mRNA expression of Nfatc1 and Rgs14 in a salt-sensitive mouse model was also reduced after exposure to a high-salt diet or induced CKD. Our study (1) demonstrates the utility of trans-ethnic fine mapping through integration of GWASs involving diverse populations with genomic annotation from relevant tissues to define molecular mechanisms by which association signals exert their effect and (2) suggests that salt sensitivity might be an important marker for biological processes that affect kidney function and CKD in humans.

Autoeficácia, características sociodemográficas e capacitação profissional: um estudo com servidores de uma Universidade Federal

Self-efficacy, the construct developed by Albert Bandura in 1977 and widely studied around the world, means the individual's belief in his own capacity to successfully perform a certain activity. This study aims to determine the degree of association between sociodemographic characteristics and professional training to the levels of Self-Efficacy at Work (SEW) of the Administrative Assistants in a federal university. This is a descriptive research submitted to and approved by the Ethics Committee of UFRN. The method of data analysis, in quantitative nature, was accomplished with the aid of the statistical programs R and Minitab. The instrument used in research was a sociodemographic data questionnaire, variables of professional training and the General Perception of Self-efficacy Scale (GPSES), applied to the sample by 289 Assistants in Administration. Statistical techniques for data analysis were descriptive statistics, cluster analysis, reliability test (Cronbach's alpha), and test of significance (Pearson). Results show a sociodemographic profile of Assistants in Administration of UFRN with well-distributed characteristics, with 48.4% men and 51.6% female; 59.9% of them were aged over 40 years, married (49.3%), color or race white (58%) and Catholics (67.8%); families are composed of up to four people (75.8%) with children (59.4%) of all age groups; the occupation of the mothers of these professionals is mostly housewives (51.6%) with high school education up to parents (72%) and mothers (75.8%). Assistants in Administration have high levels of professional training, most of them composed two groups of servers: the former, recently hired public servants (30.7%) and another with long service (59%), the majority enter young in career and it stays until retirement, 72.4% of these professionals have training above the minimum requirement for the job. The analysis of SEW levels shows medium to high levels for 72% of assistants in administration; low SEWclassified people have shown a high average of 2.7, considered close to the overall mean presented in other studies, which is 2.9. The cluster analysis has allowed us to say that the characteristics of the three groups (Low, Medium and High SEW) are similar and can be found in the three levels of SEW representatives with all the characteristics investigated. The results indicate no association between the sociodemographic variables and professional training to the levels of self-efficacy at work of Assistants in Administration of UFRN, except for the variable color or race. However, due to the small number of people who declared themselves in color or black race (4% of the sample), this result can be interpreted as mere coincidence or the black people addressed in this study have provided a sense of efficacy higher than white and brown ones. The study has corroborated other studies and highlighted the subjectivity of the self-efficacy construct. They are needed more researches, especially with public servants for the continuity and expansion of studies on the subject, making it possible to compare and confirm the results

Locus de controle e a efetividade empresarial em microempresários do estado do Rio Grande do Norte

Entrepreneurs are individuals who can transform economic and social realities by promoting development, so it became important tools in generating externalities in regions where they operate. In Brazil, 59.9% of new ventures do not reach the fourth year of life, the mortality rate of new ventures is high. The causes of mortality are numerous, and within the behavioral aspects, one is the locus of control. This study determines the degree of association between internal locus of control and achieving business success of entrepreneurs in Rio Grande do Norte who participated in the workshop EMPRETEC. The approach that studies the behavior entrepreneurs agreed that there are psychological characteristics associated with a set of values, attitudes and needs that determine the behavior and induce the entrepreneur to achieve success. Among these features is the locus of control, a skill that individuals must identify in their actions, or lack of them, the causes of their successes and failures. The locus is external when the individual attributes to factors outside themselves as causes of their results, and is built in when you can identify the actions that led to success. We surveyed 223 entrepreneurs statewide who answered the questionnaire for assessing the scale of locus of control, selfassessment questionnaire of entrepreneurial characteristics of EMPRETEC and a questionnaire assessing the business success. 71.9% were identified as having success. Among the behavioral characteristics strongest in the group of entrepreneurs are setting goals and commitment. Was found for locus of control mean value of 7.35, confidence interval between 7.05 and 7.66. Showing that the locus of control group is predominantly internal. We also found a correlation between the locus and commitment, between setting goals and commitment; calculated risks and information search; search of information and commitment, and between commitment and independence and self confidence. Dependence was not identified among the set of features and business success, determining the absence of an ideal profile. However, logistic regression significant association was found indicating that the smaller the individual's locus of control increased the likelihood of it achieving business success

Avaliação do papel dos polimorfismos em enzimas metabolizadoras e enzimas transportadoras de fármacos em neoplasias hematológicas

O cancro é um dos maiores causadores globais de mortalidade e morbilidade, ocorrendo cerca de 14 milhões de novos casos por ano e 8,2 milhões de mortes anuais com esta patologia, números que tendem a aumentar 70% nas próximas duas décadas. A característica tumoral mais nefasta é a sua capacidade de metastização para outros órgãos, um mecanismo que pode ser despoletado pela falha dos mecanismos normais de controlo de crescimento, proliferação e reparação celulares, que facilita o processo de transformação de células normais em células cancerígenas. A oncogénese processa-se em três etapas, a iniciação, a promoção e a progressão e pode ter origem em células estaminais cancerígenas, que regulam as capacidades de propagação e recidiva do tumor. As neoplasias hematológicas resultam de alterações genéticas e /ou epigenéticas que conduzem à desregulação da proliferação, ao bloqueio da diferenciação e/ou à resitência à apoptose. Para além dos fatores de risco exógenos, como agentes carcinogénicos físicos, químicos e biológicos, existem também fatores endógenos, incluindo características genéticas, que podem alterar a predisposição para o aparecimento de neoplasias, bem como influenciar a resposta à terapêutica. Uma das terapêuticas aplicadas no tratamento do cancro é a quimioterapia. Os fármacos administrados a doentes oncológicos seguem normalmente o percurso de absorção, distribuição, metabolização e eliminação. Este curso pode sofrer alterações caso as proteínas transportadoras e metabolizadoras necessárias não atuem corretamente. Para um melhor conhecimento da influência das alterações provocadas por variações nos genes que codificam proteínas transportadoras de efluxo (MDR1, MRP1), proteínas de influxo (OCTN2) e proteínas metabolizadoras (UCK2), o objetivo deste trabalho consistiu na avaliação de polimorfismos nos genes MDR1, MRP1, OCTN2 e UCK2 e da sua relação com a predisposição para o desenvolvimento de neoplasias hematológicas. Para isto, foram utilizadas amostras de 307 doentes com neoplasias hematológicas, 83 de Síndrome Mielodisplásica (SMD), 63 Leucemia Mieloide Aguda (LMA), 16 de Síndrome Mielodisplásica/Neoplasias Mieloproliferativas (SMD/NMP), 77 de Mieloma Múltiplo (MM) e 68 de Gamapatia Monoclonal de Significado Indeterminado (MGUS) e 164 de controlos não neoplásicos e/ou indivíduos saudáveis. As amostras de ADN foram extraídas do sangue periférico com protocolo adequado. De forma a determinar os genótipos correspondentes a cada amostra, realizaram-se técnicas de RFLP-PCR e ARMS-PCR. Posteriormente, calcularam-se estatisticamente as frequências alélicas e genotípicas relativas às variantes polimórficas dos genes MDR1, MRP1, OCTN2 e UCK2 e verificou-se se estavam em Equilíbrio de Hardy-Weinberg. De seguida, avaliou-se a força de associação entre as formas polimórficas e o risco de desenvolvimento de neoplasias hematológicas, através do cálculo do risco relativo por análise de regressão logística. Avaliaram-se ainda os perfis genéticos e a possível relação com o desenvolvimento e progressão da neoplasia com recurso a regressão logística e análise de Kaplan-Meier. De um modo geral as frequências alélicas e genotípicas não se revelaram alteradas comparativamente ao esperado. A análise do odds ratio associado ao polimorfismo rs1045642 do gene MDR1 revelou que o genótipo CT pode constituir um fator de risco aumentado de 1,84x para o desenvolvimento de Gamapatias Monoclonais e 2,27x para o desenvolvimento de Mieloma Múltiplo. Por outro lado, a presença de genótipos portadores do alelo T têm um efeito protetor no desenvolvimento de MM (OR=0,41). O cálculo do risco associado ao polimorfismo rs4148330 do gene MRP1 revela que o genótipo AG é um fator protetor (OR=0,50) para o desenvolvimento de LMA, assim como o alelo G (OR=0,50). Além disso, verificámos que existe uma associação de risco de desenvolver neoplasia com o polimorfismo rs2185268 do gene UCK2. De facto, a presença dos genótipos CC e AC representam um fator de risco 4,59x aumentado para o desenvolvimento de SMD/NMP. O polimorfismo rs274561 do gene OCTN2 não apresenta relação com o risco relativo de desenvolvimento neoplásico. Da avaliação da influência dos polimorfismos em estudo na sobrevivência global dos doentes, podemos assumir que a presença do genótipo GG relativo ao polimorfismo rs2185268 do gene UCK2 representa uma diminuição da sobrevivência em 11 meses. Os resultados obtidos a partir do nosso estudo permitem-nos concluir que os polimorfismos podem ser fatores relevantes na predisposição para o desenvolvimento de neoplasias hematológicas e na progressão destas doenças.

Os forrós da Serra da Gameleira (São Tomé/RN) : etnicidade, festa e sociabilidade

This work intends to describe and analyze the parties of forró that happen in Serra da Gameleira, in São Tomé/RN. Serra is a divided social space: groups of different ethnic origins live together in Gameleira de Baixo, Salgadinho (or Gameleira de Cima) and Chaves Belas. They are approximately two hundred families that live exclusively from agriculture. We try to understand how the parties inform about the social organization, the ethnic composition of the families that live there and the past of Serra, through the genealogy offorró players. In the discussion, we identify the festive places: in the total, we have Five houses of forró that function regularly one of them has been described. The private and public spaces inside them are intimate related, with no clear limits between the house of forró and the residence. Each house of forró has an owner, that regularly makes the parties, mobilizing a big part of the inhabitants, and provoking the straitening of the social relations. Observing the festive sociability between different social segments, the forró appears like na element that minimizes social conflicts, providing news ways of association and cooperation in the space of Serra da Gameleira. For the collection of facts, we used the ethnographic method, through the direct observation, interviewing and documentary research. The local history is recounted following the routes of oral memory and historical documents analysis. In the end of the analysis, we concluded that music and party are elements that aggregate the different groups that live in the location and determine forms of expression of what is seen as a traditional culture

A utilização de serviços odontológicos entre crianças e fatores associados em Sobral, Ceará, Brasil

NORO, L. R. A. et al. A utilização de serviços odontológicos entre crianças e fatores associados em Sobral, Ceará, Brasil. Cad. Saúde Pública, v. 24, n. 7, p. 1509-1516. 2008. ISSN 0102-311X.

A cultura e as características qualitativas da informação financeira: evidência empírica a partir do relato de entidades cotadas na União Europeia

Mestrado em Contabilidade