Bioquímica quântica na diferenciação dos níveis de ativação de receptores AMPA por agonistas parciais Wilardina

In the central nervous system (CNS) of mammalian, fast synaptic transmission between nerve cells is performed primarily by α-amino-3-hydroxy-5-methyl-4- isoxazolepropionic acid (AMPA) receptors, an ionotropic glutamate receptor that is related with learning, memory and homeostasis of the nervous system. Impairments in their functions are correlated with development of many brain desorders, such as epilepsy, schizophrenia, autism, Parkinson and Alzheimer. The use of willardiine analogs has been shown a powerful tool to understanding of activation and desensitization mechanisms of this receptors, because the modification of a single ligand atom allows the observation of varying levels of efficacy. In this work, taking advantage of Fluorine Willardiine (1.35Å), Hydrogen Willardiine (1.65Å), Bromine Willardiine (1.8Å) and Iodine Willardiine (2.15Å) structures co-crystalized with GluA2 with codes 1MQI, 1MQJ, 1MQH and 1MQG, we attempted to energetically differentiate the four ligands efficacy. The complexes were submitted to energetic calculations based on density functional theory (DFT), under the optics of molecular fractionation with conjugate caps (MFCC) method. Obtained results show a relationship between the energetic values and willardiines efficacy order (FW> HW > BrW > IW), also show the importance of E705, R485, Y450, S654, T655, T480 e P478 as the amino acids that contribute most strongly with the interaction of four partial agonists. Furthermore, we outlined the M708 behaviour, attracted by FW and HW ligands, and repels by BrW and IW. With the datas reported on this work, it is possible for a better understanding of the AMPA receptor, which can serve as an aid in the development of new drugs for this system.

Estimulação transcraniana por corrente contínua (ETCC) sobre o córtex motor na modulação autonômica em pessoas com lesão medular com diferentes graus e níveis de lesão

Introduction: Transcranial Direct Current Stimulation (tDCS) has been used in studies for the treatment of chronic pain, but their effects on the autonomic nervous system (ANS) are non-existent. Therefore, the need for studies is of fundamental importance, as these individuals have autonomic imbalance and the intensity of this is dependent on the degree and level of injury. Objective: We investigated the effect of tDCS on the ANS in people with spinal cord injury (SCI) with different degrees and levels of injury. Methods: Randomized, placebo-controlled, double-blind, applied anodal tDCS or sham on the primary motor cortex (M1), bilaterally. The subjects (lower incomplete injury, n = 7; lower complete injury, n = 9; and high complete thoracic injury, n = 3) visited the laboratory three times and received active or sham tDCS for 13min. The heart rate variability (HRV) was measured before, during and after stimulation and analyzed the variables LF, HF and LF / HF. Results: The tDCS modulated the ANS in different ways among the groups. In individuals with SCI high complete thoracic the tDCS did not change the HRV. However, for individuals with SCI low incomplete, tDCS changed the HRV in order to increase sympathetic (LF, p = 0.046) and reduced parasympathetic (HF, p = 0.046). For individuals SCI low complete to tDCS changed the HRV reduction sympathetic (LF, p = 0.017) and increased parasympathetic (HF, p = 0.017). Conclusions: The present study suggests that anodal tDCS applied on the motor cortex bilaterally could modulate the ANS balance in people with spinal cord injury and that this effect is dependent on the degree and level of injury.

Relação do polimorfismo BDNF val66met e níveis periféricos de BDNF com a doença de Parkinson e sua sintomatologia

Neurodegenerative diseases are frequently studied due to the increasing number of cases associated with the populational ageing and to the impact on the conditions on the quality of life. Parkinson’s disease (DP) is the second most frequent neurodegenerative disease. Despite the fact that its etiology is not completely understood, it is known that DP is caused by environmental and genetic factors. Thus, the investigation of etiologic factors and mechanisms responsible for the changes that lead to DP may help early diagnostic and prevention. A possible association between DP and the common polymorphism of Brain Derived Neurotrophic Factor (BDNF) G196A (Val66Met) has been suggested by different studies with contrasting results. For this reason, the aim of this study is to investigate if the BDNF Val66Met polymorphism is related to susceptibility to DP in a cohort of Brazilian patients. Additionaly, we verify if the presence of the polymorphism implies in alterations in the BDNF whole blood concentrations, as well as variations in symptomatology. The sample comprised Brazilian patients accompanied by the neurology service of the Onofre Lopes University Hospital (HUOL) and healthy controls (CTRL). The motor aspects of DP were evaluated by Hoehn e Yahr Scale (HY), Unified Parkinson’s Disease Rating Scale (UPDRS) and Schwab & England Scale (SE). For the evaluation of non-motor symptoms were used the following instruments: Frontal Assessment Battery (BAF), Mini-Mental State Examination (MEEM), Beck Depression Inventory (IDB) and the Beck Anxiety Inventory (IAB). Blood samples were collected for BDNF Val66Met polymorphism genotyping and BDNF whole blood measurement. As expected, DP patients performed worse in motor, cognitive and emotional battery of questionnaires. Alleles distribution between DP and CTRL was not significantly different, but the A/G genotype was significantly associated with a protector factor for DP. In contrast, the G/G genotype was significantly associated with depression and anxiety development in DP patients. However, BDNF concentrations were not different between genotypes or groups. This is the first study of genetic association of this polymorphism with DP in Brazilian subjects and the first one that associate A/G genotype with protection against DP.

Frequência de microrganismos em conteúdo vulvovaginal de vacas tratadas com dispositivos intravaginais de progesterona

The dissertation was divided in two studies. With the first, aimed to evaluate the occurrence of microorganisms present in the vulvovaginal region of cows that received intravaginal progesterone devices during the fixed-time artificial insemination (FTAI) programs, and correlate the results with pregnancy rates. Samples were collected from vulvovaginal region of 30 beef cows Guzerá and 30 crossbred dairy cows, and also intravaginal devices, randomly. Of the 120 samples of cows, 60 corresponded to the collections of the period prior to the introduction device (D0) and 60 to the subsequent withdrawal of it (D9); it yielded 100% of bacterial growth, whereas, in most samples, it was found more than one isolated. In D0, the most frequent agent was Escherichia coli (52%), and in D9, Proteus spp and E. coli were the most frequent (32% and 28%, respectively). Regarding intravaginal progesterone devices, in D0 were isolated 37 microorganisms, being predominant those of the genus Bacillus (35%); in D9, 41 colony forming units (CFU) were isolated, of which 36.6% corresponded to Proteus spp. For the analysis of the antimicrobial profile, susceptibility testing was performed by diffusion agar disk, and cows that did not became pregnant after FTAI program were selected, as a future treatment. There resistance 100% to penicillin, and sensitivity, approximately, 90% to gentamicin, both isolates obtained from samples of beef cows and obtained of dairy cows. Regarding pregnancy rate, the 30 beef cows, 11 were diagnosed pregnant (36.7%), 4 (36.4%) treated with reused devices and 7 (63.6%) with new devices, which showed more effective. Of the 30 dairy cows, 15 were pregnant (50%), 8 (53.3%) were implanted with reused devices and 7 (46.7%) with new devices, with no significant differences in pregnancy rates. Because it is a research, females were chosen at random, and factors such as body condition, nutritional management and health weren't priority. With the second study, aimed to analyze the similarity between strains, conducted by technical Random Amplified Polymorphic DNA (RAPD -PCR). Presence of E. coli and the absence of pregnancy were selection criteria used. From the results, it was observed that most of the isolates wasn't phylogenetically similar, since they showed lower than 85% similarity. The study stressed the importance of E. coli in vulvovaginal microbiota of cows and the presence of phenotypic and genotypic characters of this bacterium on possible reproductive problems.

Sincronización de la ovulación y el ciclo inducido por el efecto "macho" mediante la administración de progesterona por vía intravaginal en cabras en período de anestro estacional

Algunos de los métodos alternativos a los tratamientos hormonales clásicos utilizando progestágenos y eCG, para la inducción y sincronización del celo y la ovulación, unido a la inseminación artificial (IA) a tiempo fijo en ganado caprino, están basados como el IMA.PRO2® en el estímulo por la presencia de los machos (efecto macho) y la sincronización de la ovulación inducida mediante prostaglandina F2α o sus análogos. En estos métodos, la administración de una dosis baja de progesterona o progestágenos durante un breve periodo de tiempo en el momento de la introducción de los machos, es un método efectivo para evitar la presencia de cuerpos lúteos de mala calidad que generan ciclos cortos, consiguiendo un primer ciclo inducido de adecuada función luteal. En el método IMA-PRO2® en el momento de la introducción de los machos se administran 25 mg de progesterona por vía intramuscular en vehículo oleoso, con buenos resultados de fertilidad después de la administración de cloprostenol y la IA sistemática, sin embargo las directivas europeas han marcado nuevas restricciones a la administración de esteroides en animales de producción, que en el caso de la progesterona o progestágenos sólo son permitidas por vía intravaginal. Se diseñaron cuatro experimentos con el fin de desarrollar una administración de progesterona por vía intravaginal, utilizable en la práctica a nivel de campo y con una eficacia similar en términos de fertilidad, en el conjunto del método IMA-PRO2®...

O uso da PCR em tempo real para o estudo da carga parasitária e dos níveis transcricionais durante a infecção experimental por Trypanosoma cruzi

Trypanosoma cruzi is causative agent of Chagas disease, one of most neglected tropical diseases. Estimated that about 11 million people worldwide are infected by T. cruzi and about 6 to 7 million people are at risk in endemic areas. During the process of invasion of host and parasite interact enabling signal transduction and gene expression modulation in response to invasion. The diversity of activated proteins and pathways to repair the damage by disruption of the plasma membrane interest to us and thus present study developed a new form of detection and quantitation by polymerase chain reaction in real time (qPCR) of parasitic load T. cruzi and quantified transcriptional levels relative (RT-qPCR) of dysferlin, Sphingomyelin acid esferase (ASM), transcription factor EB (TFEB) Galectins 1 and 3 and Annexin A2. This study demonstrated that quantification by real time PCR using primers P21fw and P21rv was specific and sensitive for detection of T. cruzi in vivo and in vitro, as well as transcriptional levels of genes related to cytoskeletal organization and repair plasma membrane are modulated in response to damage generated by parasite.

Análise da Indisciplina de uma Escola Básica Integrada à luz da teoria dos níveis de indisciplina de João Amado

Trabalho com o tema “A Relação da Indisciplina de uma Escola Básica Integrada no quadro da teoria dos níveis de indisciplina de João Amado” realizado no âmbito do Mestrado de Gestão, Avaliação e Supervisão Escolar. Para abordar este tema iremos começar por abordar os conceitos de indisciplina e de delinquência na escola, seguidamente iremos aprofundar as relações de controlo e de poder dentro da sala de aula. Posteriormente será abordada a problemática da dimensão das turmas através de estudos realizados em alguns dos países do anel de fogo do pacífico como a China, o Japão, entre outros. Depois iremos abordar o comportamento dos professores e alunos relacionado com a composição das turmas. Posteriormente será apresentada a investigação realizada sobre a indisciplina de uma Escola Básica Integrada da Região Autónoma dos Açores, onde vamos procurar analisar se existe alguma relação entre a forma como uma escola age relativamente à indisciplina e as investigações existentes, especificamente as investigações sobre a teoria dos níveis de indisciplina de João Amado tanto como ao nível do que é considerado indisciplina pela escola e se estão enquadrados com os níveis enunciados e compreender se as sanções correspondentes estão de acordo com a gravidade de acordo com cada um dos três níveis expostos por João Amado. Por fim vamos apresentar as nossas conclusões sobre o caso em estudo.

A Influência de um Curso de Meditação nos Níveis de Mindfulness, Satisfação com a Vida e Optimismo

O mindfulness tem sido alvo de um crescente interesse no domínio da Psicologia e a sua importância ao nível do funcionamento psicológico tem vindo a ser empiricamente verificada. Os estudos sobre mindfulness têm demonstrado que este constructo está positivamente relacionado com a saúde psicológica e negativamente com a psicopatologia. Deste modo, a presente investigação teve como objectivo compreender a relação entre mindfulness, satisfação com a vida e optimismo, bem como explicar a influência de um curso de meditação nestas variáveis. Neste sentido, os participantes completaram uma bateria de questionários de auto-resposta avaliando o mindfulness, satisfação com a vida e optimismo num primeiro momento de avaliação, antes do curso de meditação (N = 104), num segundo momento, depois do curso de meditação (N = 30). Os resultados revelam um aumento significativo nos níveis de mindfulness, satisfação com a vida e optimismo após a frequência do curso de meditação e indicam que a prática do curso de meditação fortalece a relação entre estes constructos. Apesar de algumas limitações metodológicas que futura investigação deverá tentar colmatar, este estudo reforça a importância da prática de meditação para um funcionamento psicológico positivo e adaptativo. / Mindfulness has been target of a growing interest to Psychology in terms of the verified importance to psychological function. Studies show that mindfulness has demonstrated an important correlation with psychological health and negatively to psychopathology. The objective of the present research is to understand the relation between mindfulness, satisfaction with life and optimism as well as to explain the influence of a course of meditation in the study of these variables. The participants of this research completed several different types of auto-response questionnaires regarding mindfulness, satisfaction with life, and optimism; prior to the meditation course (N = 104), and then, after the meditation course (N = 30). Therefore, the results of the research demonstrated that the level of mindfulness, satisfaction with life and optimism significantly increased as the participants undergone in the meditation course as well as indicated that the practice such course strengthens the relation between the variables. Despite some methodological limitations that a future research should relieve, this study reinforces the practice of meditation for a positive and adaptive psychological function.

Stress e Emergência Médica: níveis e factores de stress em profissionais de emergência pré–hospitalar

O stress é a doença da actualidade, devido à exigência e à pressão emocional que o meio pode exercer sobre todas as pessoas, quer no seu ambiente pessoal quer no ambiente profissional. O termo stress serve cada vez mais como justificação ou forma de expressão de irritações, mal-estar físico e mental, cansaço, ansiedade e depressões. Todavia torna-se pertinente percebermos a evolução do stress enquanto conceito e enquanto doença. Neste trabalho contextualizamos o stress no âmbito da Emergência Pré – Hospitalar, explorando-o, avaliando-o e analisando-o, de modo a podermos perceber os níveis de stress e de que forma os Profissionais de Emergência a ele estão sujeitos. Considerando a base teórica deste estudo definimos, como objectivo geral do trabalho, a avaliação de níveis e factores de stress percepcionados pelos profissionais de emergência médica e como objectivos específicos do estudo a descrever e caracterizar, a nível da região centro, os indivíduos que desempenham funções na emergência préhospitalar, verificar a percepção dos mesmos no que respeita ao stress na sua vida profissional, conhecer como percepcionam os níveis de stress em situações profissionais de emergência e identificar a sua percepção no que concerne aos níveis de stress diário no seu quotidiano. Com estes objectivos, utilizamos uma amostra de 184 profissionais de Emergência Médica divididos por quatro profissões [Médicos, Enfermeiros, Técnicos de Ambulância de Emergência (TAE) e Operadores de Central de Emergência Médica(OPCEM)], com idades compreendidas entre os 20 e os 56 anos. Realizámos ainda um estudo descritivo explorando os questionários de stress no INEM (Instituto Nacional de Emergência Médica) e o Questionário de Vulnerabilidade do Stress com variáveis do estudo demográfico. Podemos concluir que o stress está presente nas actividades de emergência. A sua presença relaciona-se, por um lado, com uma maior ou menor discriminação dos factores stressantes e, por outro, numa maior ou menor centração no sujeito, nomeadamente, um maior perfeccionismo e intolerância à frustração que se apresentam como distressantes para a actividade profissional de emergência. Os aspectos estudados permitem-nos clarificar o sentido e a utilidade do conceito, a sua operacionalidade e a sua importância nos profissionais de emergência. / Stress is the disease of our time, due to the requirement and emotional pressure that the media can have on all people, both in their personal environment and in the professional environment. The word stress increasingly serves as a justification or form of expression of irritation, physical discomfort and mental fatigue, anxiety and depression. However it is pertinent to realize the evolution of stress as a concept and as a disease. In this work, we contextualize under the stress of the Pre - Hospital Emergency, exploring it, assessing it and analyzing it, so you can see the stress levels and how the Emergency Professionals are subject to it. Whereas the theoretical basis of this study as a strategic objective of the work, the assessment of levels and stress factors perceived by medical emergency professionals and specific objectives of the study description and characterization, the level of the center, individuals who perform functions in pre-hospital emergency, check their perception with regard to the stress in your life, know how they experience the stress levels of professionals in emergency situations and identify the perceptions of individuals regarding the levels of daily stress in their daily lives. For this we used a sample of 184 professionals of Medical Emergency divided by four professions [(Doctors, Nurses, Technicians Ambulance Emergency (TAE), Operators of Emergency Medical Center (OPCEM)], aged 20 to 56 years. We conducted a descriptive study also exploring the questionnaires of stress in INEM(National Institute of Medical Emergency) and Vulnerability Questionnaire Stress with variables of the study population. We can conclude that stress is present in emergency activities. Its presence is related to, first, with more or less stressful factors of discrimination and, secondly, a greater or lesser concentration on the subject, in particular, greater perfectionism and intolerance to frustration that stand as unstressed for the activity of emergency professionals. The areas studied will clarify the meaning and usefulness of the concept, its operation and its importance in day-to-day occupation of emergency.

Obtenção de altos níveis séricos de endostatina murina em camundongos pela utilização de células de ovário de hamster chinês recombinantes secretando endostatina transplantadas em dispositivos de imunoisolamento

Dissertacao (Mestrado)

Níveis de cálcio e relação cálcio: fósforo em rações para poedeiras leves de 24 a 40 semanas de idade

v. 17, n. 2, p. 206-216, abr./jun. 2016.

Respuesta ovárica y niveles hormonales en vacas Holando en diferentes estados reproductivos tratadas con Ovsynch y dos formulaciones de progesterona

Resumen Para evaluar los efectos de una progesterona (P4) inyectable en suspensión oleosa en tratamientos de sincronización de la ovulación se realizaron tres experimentos en vacas Holando multíparas en distinto estado reproductivo: 1) 24 vacas con más de 140 días posparto (DPP), en anestro, en tres grupos: Ovsynch + CIDR, Ovsynch + 200 mg y Ovsynch + 400 mg de MAD-4; 2) 8 vacas en anestro con menos de 100 DPP en dos grupos: Ovsynch + CIDR y Ovsynch + 200 mg de MAD-4; y 3) 15 vacas repetidoras ciclando con más de 200 DPP en dos grupos: Ovsynch + 200 y Ovsynch + 400 mg de MAD-4. En todos los experimentos se realizaron sangrados frecuentes y ultrasonografías ováricas. En el experimento 1 el grupo CIDR mantuvo niveles de P4 constantes en 1,5±0,7 ng/mL; los grupos MAD 200 y MAD 400 alcanzaron niveles de P4 superiores a 10 ng/mL (P<0,05) a las 8 h, descendiendo a las 48 h a valores basales. El diámetro del folículo dominante (DF) al día 9 fue 5 mm superior en el grupo CIDR que en los grupos MAD (P<0,05). En los experimentos 2 y 3 los niveles de P4 fueron similares a los del experimento 1, así como la diferencia del diámetro del DF al día 9. Se concluyó que los niveles de P4 circulantes luego de la administración de P4 inyectable aumentan excesivamente al comienzo del tratamiento, disminuyendo rápidamente a las 48 h, lo que además de ser menos efectivo como fuente de P4 en tratamientos de inducción de ovulación que la administración intravaginal, afectan el diámetro del folículo dominante inducido.

Desempenho de sulcadores no desenvolvimento e produtividade da soja em solo submetido a diferentes níveis de compactação

On the national scene, soybean crop occupies a prominent position in cultivated area and volume production, being cultivated largely in the no tillage system. This system, due to the intense traffic of machines and implements on its surface has caused soil compaction problems, which has caused the yield loss of crops. In order to minimize this effect the seeder-drill uses the systems to opening the furrow by shank or the double disc type. The use of the shank has become commonplace for allowing the disruption of the compacted surface layer, however requires greater energy demand and may cause excessive tillage in areas where there is not observed high levels of compaction. Thus, this study aimed to evaluate the effects of furrowers mechanisms and levels of soil compacting on traction requirement by a seeder-drill and on the growing and productivity of soybean in an Oxisol texture clay, in a two growing seasons. The experimental design consisted of randomized blocks with split plots with the main plots composed of four levels of soil compaction (N0 – no tillage without additional compaction, N1, N2 and N3 – no tillage subjected to compaction through two, four and six passes with tractor, respectively) corresponding to densities of soil 1.16, 1.20, 1.22 and 1.26 g cm-3, and subplots by two furrowers mechanisms (shank and double disc) with four replicates. To evaluate the average, maximum and specific traction force requested by the seeder-drill, was used a load cell, with capacity of 50 kN and sensitivity of 2 mV V-1, coupled between the tractor and seeder-drill, whose data are stored in a datalogger system model CR800 of Campbell Scientific. In addition, were evaluated the bulk density, soil mechanical resistance to penetration, sowing depth, depth and groove width, soil area mobilized, emergence speed index, emergence operation, final plant stand, stem diameter, plant height, average number of seeds per pod, weight of 1,000 seeds, number of pods per plant and crop productivity. Data were subjected to analysis of variance, the mean of furrowers were compared by Tukey test (p≤0.05), while for the factor soil compaction, polynomial regression analysis was adopted, selected models by the criterion of greater R2 and significance (p≤0.05) of equation parameters. Regardless of the crop season, penetration resistance increase as soil compaction levels up to around 0.20 m deep, and bulk density influenced the sowing quality parameters, however, did not affect the crop yield. In the first season, there was a higher productivity with the use of the shank type. In the second crop season, the shank demanded greater energetic requirement with the increase of bulk density and opposite situation with the double disc. The locking of sowing lines allow better performance of the shank to break the compacted layer.

Os níveis de desenvolvimento do pensamento geométrico: o aprendizado do conteúdo de polígonos numa perspectiva do modelo Van Hiele

This work studies the van Hiele model, the levels of development of geometric thinking and its learning phases. Using this knowledge, we prepared a Research Instrument to identify the Level of Development in Geometric Thinking (Levels of van Hiele) of Middle School students, related to contents of Polygons. We have applied this Research Instrument to 237 students from a public school (state) in Curitiba, and we made an analysis of the acquired data. We have improved the Instrument’s questions so that it can be used by teachers during the class. Helping to identify to which level content the student belongs, related to the proposed.

Influência das etapas do processo de abate de suínos na prevalência de patógenos e níveis de microrganismos indicadores de qualidade e higiene

The pig slaughter process involve different steps that can influence the microbiological quality of carcasses. At this, the understanding of the slaughter process on the microbiological aspects is necessary for the implementation and evaluation of critical control points. The microbiological control of the slaughter process should involve the evaluation of pathogens prevalence and levels of quality and hygiene indicator microorganisms. This study aimed at investigating the influence of steps slaughter process on the microbiological levels of pig carcasses, and evaluate if there is correlation between pathogens (Salmonella spp. and Listeria monocytogenes) and indicators (aerobic mesophilic counts, total coliforms, Escherichia coli and Enterobacteriaceae) microorganisms. A high Salmonella soroprevalence in pigs were founded before the slaughter (57.49 %). While the Salmonella prevalence in carcasses at the initial stage of the slaughter was 26.67 % and in the final stage 1.11 %, L. monocytogenes was detected only in the final washing and cooling steps, with a prevalence of 21.11 and 8.89 %, respectively. The aerobic mesophilic counts, Enterobacteriaceae, total coliforms and E. coli levels in initial steps of slaughter process were 4.25 ± 0.37; 1.25 ± 0.38; 1.10 ± 0.35 and 0.86 ± 0.36, respectively. At the end of slaughter process the results were lower (ranging from 0.16 at 2.70 log CFU/cm2). The step that most reduced microbiological levels was the scalding. The dehairing was a critical step that led to a significant increase of microorganisms levels in the process (p < 0.05). The evisceration not proved to be a critical step on the increase of microbial levels, differently of the final washing, which showed significant increases (p < 0.05) over the levels of aerobic counts, total coliforms, E. coli and enterobacterias (0.30; 0.36; 0.27 and 0.42 log respectively) and Salmonella spp. and L. monocytogenes. The chilling contributes significantly to the reduction of microbiological levels of carcasses, bringing them to levels below the all process stages, with the exception of scalding. No correlation between the hygiene indicator microorganisms used and presence of Salmonella spp. and L. monocytogenes were obtained (p < 0.05). The results show that steps in the process are critical to the sanitary profile, which implies the need to implement actions in the process to reducing the microbiological levels.