Un dios que se transforma en héroe : Una aproximación genética a los textos de Peter Pan

Desde la perspectiva de la genética textual, en este trabajo observamos las representacionesdel personaje de Peter Pan en los textos de J. M. Barrie dedicados a este con respecto a sussemejanzas con el dios griego Pan, para ver de qué manera dialogan con los diferentes sentidosque el dios fue connotando a través de siglos. Analizamos la fotonovela The Boys Castawaysof Black Lake Island (1901); The Little White Bird (1902), novela en la que aparece en seiscapítulos; las "Fairy Notes" (1903) en borrador y el libreto inédito llamado Anon: a play de la obra teatral Peter Pan or The Boy Who Would Not Grow Up (estrenada en 1904); la novela Peter Pan in Kensington Gardens (1906), que consiste en esos seis capítulos de The LittleWhite Bird; la novela Peter and Wendy (1911), adaptación de la obra de teatro; un guion cinematográfico,Scenario for a Proposed Film of Peter Pan (c. 1918), que no se filmó; y ellibreto de la obra, finalmente publicado, con bastantes cambios, en 1928

A Engenharia Genética como Proposta de Co-Criação vista a Partir do Pensamento do Teólogo Ronald Cole-Turner

Esta dissertação de mestrado busca pesquisar a relação estabelecida pelo teólogo Ronald Cole-Turner, entre a engenharia genética e o conceito de co-criação , desenvolvido como uma alternativa para que se pense o papel do ser humano frente aos desenvolvimentos tecnológicos atuais. Tendo em vista o conceito da evolução, da criação contínua e dos potenciais gerados pela engenharia genética, Cole-Turner defende que o ser humano pode participar das atividades criativas de Deus por meio desta tecnologia, desde que priorize os aspectos redentivos que podem ser extraídos de seu uso. Faz isto em demonstrar, por meio do relato Jawista da criação, como o agir criativo de Deus está relacionado ao agir humano, através de suas tecnologias, e em relacionar os milagres de cura operados por Jesus nos evangelhos, como explicitação epistêmica da vontade de Deus, que pode ser participada pelos seres humanos. O objetivo básico desta pesquisa será o de relatar, no primeiro capítulo, o aspecto teórico da engenharia genética, nas suas possibilidades positivas e negativas. Em seguida, no capítulo segundo, se fará uma exposição do pensamento de Cole- Turner, em diálogo com outros interlocutores do tema. Por fim, no último capítulo, se verificará as respostas oferecidas às várias observações críticas que seu pensamento recebe. A hipótese é a de que, se a criação é um projeto contínuo e inacabado, e o destino dos seres humanos se encontra inserido dentro desta continuidade, seria coerente afirmar que estes possam participar das atividades de Deus em tais projetos. Como a evolução biológica é uma dimensão da criação, que ocorre por meio de transformações no capital genético dos organismos vivos, os seres humanos, tendo conhecimento e habilidade para repetir os processos verificados nestas modificações, poderão empreender seus esforços, tanto na área da técnica, como na da ética, para participarem da consecução dos propósitos de Deus.(AU)

ANÁLISE DA EPISTEMOLOGIA-ONTOLÓGICA DE PAUL TILLICH: O Princípio de Identidade e implicações para o método e saber teológico

A pesquisa analisa e epistemologia ontológica de Paul Tillich. Considerando a influência de F. W. J. Schelling pretende-se destacar o lugar de certas premissas ontológicas que fundamentaram o pensamento de Tillich, principalmente o Princípio de Identidade , como um dos princípios epistemológicos chave. Partindo do pressuposto de que sua ontologia depende do Princípio de identidade a fim de que possa relacionar essência e existência, destaca-se a identidade substancial entre teologia e filosofia, permitindo melhor compreensão da relação entre ontologia e teologia, caracterizando de forma geral tanto a noção teológicofilosófica da experiência de finitude - choque ontológico como sua relação com as importantes noções de Deus como o Ser-em-si, e ultimate concern enquanto categoria ontológico-teológica. A seguir, críticas voltadas para sua construtividade ontológica serão destacadas e analisadas em termos de seus pressupostos e em seu poder de alcance. Neste âmbito crítico, considerar-se-á breve comparação entre a tentativa do sistema ontológicoteológico de Tillich e os sistemas de Kant e Hegel, com o objetivo de apresentar paralelos críticos entre a postura de tais sistemas no campo epistemológico e ontológico, e suas influências sobre a teologia. Ao final, pretende-se entender as implicações de sua ontologiateológica tanto para a forma metodológica de correlação entre situação e resposta, como na relação com outros sistemas teológicos, que possibilitaram ou não, mediações entre cultura e teologia. Neste segmento, notar-se-á como uma das implicações da ontologia-teológica de Tillich, o imprescindível retorno do saber teológico entre os demais saberes delineadores da realidade; considerando-se certas possibilidades críticas a partir de seu pensamento com respeito ao uso da razão instrumental, ética e espiritualidade.

Conservação genética da orquídea Cattleya granulosa Lindley

Cattleya granulosa Lind is a large and endemic orchid in Atlantic Forest fragments in Northeast Brazil. The facility of collecting, uniqueness of their flowers, which have varying colors between green and reddish brown, and distribution in coastal areas of economic interest make their populations a constant target of predation, which also suffer from environmental degradation. Due to the impact on their populations, the species is threatened. In this study, we evaluate the levels of spatial aggregation in a preserved population, analyze the phylogenetic relationships of C. granulosa Lindl. with four other Laeliinae species (Brassavola tuberculata, C. bicolor, C. labiata and C. schofieldiana) and also to evaluate the genetic diversity of 12 remaining populations of C. granulosa Lindl. through ISSR. There was specificity of epiphytic C. granula Lindl. with a single host tree, species of Eugenia sp. C. granulosa Lindl. own spatial pattern, with the highest density of neighbors within up to 5 m. Regarding the phylogenetic relationships and genetic patterns with other species of the genus, C. bicolor exhibited the greatest genetic diversity (HE = 0.219), while C. labiata exhibited the lowest level (HE = 0.132). The percentage of genetic variation among species (AMOVA) was 23.26%. The principal component analysis (PCA) of ISSR data showed that unifoliate and bifoliolate species are genetically divergent. PCA indicated a close relationship between C. granulosa Lindl. and C. schofieldiana, a species considered to be a variety of C. granulosa Lindl. by many researchers. Population genetic analysis using ISSR showed all polymorphic loci. The high genetic differentiation between populations (ФST = 0.391, P < 0.0001) determined the structure into nine groups according to log-likelihood of Bayesian analysis, with a similar pattern in the dendrogram (UPGMA) and PCA. A positive and significant correlation between geographic and genetic distances between populations was identified (r = 0.794, P = 0.017), indicating isolation by distance. Patterns of allelic diversity suggest the occurrence of population bottlenecks in most populations of C. granulosa Lindl. (n = 8). Genetic data indicate that enable the maintenance of genetic diversity of the species is complex and is directly related to the conservation of different units or groups that are spatially distant.

Uma análise da formação de professores de física do IFRN a partir da epistemologia de Ludwik Fleck

This study arises in the context of physics teacher training and aims, from the speech of the teacher trainer, identify possible pedagogical models and characterize thinking styles present in the course of licentiate in physics of IFRN using the epistemology of Ludwik Fleck. We classify our research as qualitative with an empirical nature, and for the analysis we chose the discursive textual analysis - DTA (MORAES, 2003). The locus of our research will be the licentiate in physics at the Federal Institute of Education, Science and Technology of Rio Grande do Norte - IFRN, Natal-Central Campus and the research subjects, a group of teacher trainers of this course. We interviewed ten teachers, being six from the group dedicated to physics and four from the group dedicated to didactics and pedagogy. From this design, we performed data acquisition consisted of: 1) semi-structured interview, 2) document analysis. On the data analysis, with the support of pedagogical trends that were observed in our study based on the perception of the similarities and differences between the ideas presented by teachers about: education and teaching; ideal teaching practice, teacher's role, learning conceptions, and according to the student and on the ideological thinking of these former teachers on the professional profile of graduates, we noted subsidies to identify evidences of the presence of three distinct thinking styles that interrelate with each other in a considerably intense way. The relevance of the study is presented in the understanding of thinking styles that participate in the dynamics of the course of teacher training in physics, and by consequence, elucidation of a problem pointed out a priori as motivating the research: the difficulty of communicative interaction on educational practices among teacher trainers. We bring Fleck's epistemology as a motivating possibility of dialogue and negotiation, setting thus an instrument of real change, towards the significance of teacher training in physics.

Desenvolvimento de marcadores microssatélites e estrutura genética espacial da Copernicia prunifera (Miller) H. E. Moore (Arecaceae)

The present study aimed to develop microsatellite markers (SSR) for Copernicia prunifera; and characterize the demographic pattern and the spatial genetic structure (SGS) in different development stages of C. prunifera in a natural population of Rio Grande do Norte (RN) by using ISSR molecular markers. 17 SSR primers pairs were developed, which were tested by using DNA from samples of different populations. The demographic and genetic spatial structure was assessed in a plot with an area of 0.55 ha, where all individuals were georeferenced. The molecular analyses with the use of microsatellite markers pointed out that all built primers pairs, when submitted to PCR, had amplification. They showed sizes of base pairs ranging between 113 and 250 bp. The demographic analyses showed a clustered standard of spatial distribution in the first distance classes, random between 40 and 50 m and segregated in higher distances. Eight ISSR primers were used, thereby producing a total of 102 loci, with 100 of them being polymorphic. Among the three stages, the young showed the highest Nei’s genetic diversity index (He = 0.37); whilst the lowest index was found in the reproductive adults (He = 0.34). The AMOVA results showed a greater genetic differentiation within the development stages (98.61%) in comparison to the interval among the stages (1.39%). The total population (n = 161) showed a positive and significant relationship of kinship in the first distance class (12.3 m). The young showed a significant kinship up to 10.5 m and negative in the fifth distance class (37.6 m). The non-reproductive adults had a positive relationship of kinship in the first distance class (11.0 m) and random distribution of genotypes in the remaining classes. The reproductive adults showed genotypes spatially distributed in a random way. The values for the genetic bottleneck tests proved that the number of loci with excess observed heterozygosity was greater than expected. The SGS results reflect the restricted dispersion of the species, and the bottleneck tests reflect the reduction genotypes provoked by the anthropization of natural environments of C. prunifera.

Estudo e desenvolvimento de algoritmos criptográficos para redes de sensores sem fio, utilizando técnicas de programação genética

Cryptography is the main form to obtain security in any network. Even in networks with great energy consumption restrictions, processing and memory limitations, as the Wireless Sensors Networks (WSN), this is no different. Aiming to improve the cryptography performance, security and the lifetime of these networks, we propose a new cryptographic algorithm developed through the Genetic Programming (GP) techniques. For the development of the cryptographic algorithm’s fitness criteria, established by the genetic GP, nine new cryptographic algorithms were tested: AES, Blowfish, DES, RC6, Skipjack, Twofish, T-DES, XTEA and XXTEA. Starting from these tests, fitness functions was build taking into account the execution time, occupied memory space, maximum deviation, irregular deviation and correlation coefficient. After obtaining the genetic GP, the CRYSEED and CRYSEED2 was created, algorithms for the 8-bits devices, optimized for WSNs, i.e., with low complexity, few memory consumption and good security for sensing and instrumentation applications.

Estudo e desenvolvimento de algoritmos criptográficos para redes de sensores sem fio, utilizando técnicas de programação genética

Cryptography is the main form to obtain security in any network. Even in networks with great energy consumption restrictions, processing and memory limitations, as the Wireless Sensors Networks (WSN), this is no different. Aiming to improve the cryptography performance, security and the lifetime of these networks, we propose a new cryptographic algorithm developed through the Genetic Programming (GP) techniques. For the development of the cryptographic algorithm’s fitness criteria, established by the genetic GP, nine new cryptographic algorithms were tested: AES, Blowfish, DES, RC6, Skipjack, Twofish, T-DES, XTEA and XXTEA. Starting from these tests, fitness functions was build taking into account the execution time, occupied memory space, maximum deviation, irregular deviation and correlation coefficient. After obtaining the genetic GP, the CRYSEED and CRYSEED2 was created, algorithms for the 8-bits devices, optimized for WSNs, i.e., with low complexity, few memory consumption and good security for sensing and instrumentation applications.

Variabilidade genética de Leishmania spp. circulantes entre seres humanos e cães e infecção de flebotomíneos em áreas endêmicas para as leishmanioses no Rio Grande do Norte

Leishmania infantum is the main etiologic agent of visceral leishmaniasis in the New World. The pattern of distribution of leishmaniasis has changed substantially and has presented an emerging profile within the periphery of the Large Urban Centers. Leishmania infection can compromise skin, mucosa and viscera. Only 10% of the individuals infected develop the disease and 90% of human infection is asymptomatic. The main factors involved in the development of the disease are the host immune response, the vector’s species and the parasite’s genetic content. The sequencing of Leishmania isolated seeks to increase the understanding of the symptoms of individuals. The aim of this study was to evaluate the genetic diversity of circulating Leishmania strains among humans, and symptomatic and asymptomatic, and dogs from endemic areas of Rio Grande do Norte State and analyze sandflies from endemic areas for cutaneous and visceral disease. The genetic variability was evaluated by the use of markers hsp70 , ITS1 and a whole genome sequencing was also carried out. The amplified hsp70 and ITS1 of samples were analyzed and assembled using a Phred / Phrap package. The dendograms were constructed using the same methodology, but adding 500 bootstraps, followed by inferences on the relationships between Leishmania variants. The sequences of the 20 Brazilian isolates were mapped to the reference genome L. infantum JPCM5, using the Bowtie2 program and the identification of 36 contigs. The information of the valid SNPs were used in the PCA. SNPs were visualized by Geneious 7.1 and IGV. The genome annotations were transferred to their respective chromosomes and displayed on Geneious. The matching sequences of all chromosomes were aligned using Mauve. The phylogenetic trees were calculated according to maximum likelihood and JTT models. Sandflies were analyzed by PCR for the identification of Leishmania infection, a blood meal source and GAPDH sand fly. As a result, hsp70 and ITS1 were not capable of identifying genetic variability among human isolates from symptomatic and asymptomatic, and dogs. The complete sequencing of the 20 Brazilian isolates revealed a strong similarity between the circulating Leishmania strains in Rio Grande do Norte. The isolates collected in the city of Natal from humans and canines remained grouped in all analyzes, suggesting that there is genotypic and geographic proximity among the isolates. The isolated samples in the 1990s had a higher genotypic diversity when compared to freshly isolated samples. All isolates presented 36 chromosomes with variable ploidy among them, no correlation was found between the number of amastina genes copies, gp63, A2 and SSG with such clinic forms. In general, we did not find correlation between symptomatic and asymptomatic clinical forms and the gene content of the Brazilian isolates of Leishmania. 34,28% of the sandflies collected in the upper west region were L. longipalpis and the main sources of blood meal were humans, dogs and chickens.

Variabilidade genética de Leishmania spp. circulantes entre seres humanos e cães e infecção de flebotomíneos em áreas endêmicas para as leishmanioses no Rio Grande do Norte

Leishmania infantum is the main etiologic agent of visceral leishmaniasis in the New World. The pattern of distribution of leishmaniasis has changed substantially and has presented an emerging profile within the periphery of the Large Urban Centers. Leishmania infection can compromise skin, mucosa and viscera. Only 10% of the individuals infected develop the disease and 90% of human infection is asymptomatic. The main factors involved in the development of the disease are the host immune response, the vector’s species and the parasite’s genetic content. The sequencing of Leishmania isolated seeks to increase the understanding of the symptoms of individuals. The aim of this study was to evaluate the genetic diversity of circulating Leishmania strains among humans, and symptomatic and asymptomatic, and dogs from endemic areas of Rio Grande do Norte State and analyze sandflies from endemic areas for cutaneous and visceral disease. The genetic variability was evaluated by the use of markers hsp70 , ITS1 and a whole genome sequencing was also carried out. The amplified hsp70 and ITS1 of samples were analyzed and assembled using a Phred / Phrap package. The dendograms were constructed using the same methodology, but adding 500 bootstraps, followed by inferences on the relationships between Leishmania variants. The sequences of the 20 Brazilian isolates were mapped to the reference genome L. infantum JPCM5, using the Bowtie2 program and the identification of 36 contigs. The information of the valid SNPs were used in the PCA. SNPs were visualized by Geneious 7.1 and IGV. The genome annotations were transferred to their respective chromosomes and displayed on Geneious. The matching sequences of all chromosomes were aligned using Mauve. The phylogenetic trees were calculated according to maximum likelihood and JTT models. Sandflies were analyzed by PCR for the identification of Leishmania infection, a blood meal source and GAPDH sand fly. As a result, hsp70 and ITS1 were not capable of identifying genetic variability among human isolates from symptomatic and asymptomatic, and dogs. The complete sequencing of the 20 Brazilian isolates revealed a strong similarity between the circulating Leishmania strains in Rio Grande do Norte. The isolates collected in the city of Natal from humans and canines remained grouped in all analyzes, suggesting that there is genotypic and geographic proximity among the isolates. The isolated samples in the 1990s had a higher genotypic diversity when compared to freshly isolated samples. All isolates presented 36 chromosomes with variable ploidy among them, no correlation was found between the number of amastina genes copies, gp63, A2 and SSG with such clinic forms. In general, we did not find correlation between symptomatic and asymptomatic clinical forms and the gene content of the Brazilian isolates of Leishmania. 34,28% of the sandflies collected in the upper west region were L. longipalpis and the main sources of blood meal were humans, dogs and chickens.

Características de virulência, resistência e diversidade genética de sorovares de Salmonella com impacto na saúde pública, isolados de frangos de corte no Brasil /

Salmonella Enteritidis, S. Typhimurium and S. Infantis are often associated with cases of human infections worldwide and is transmitted through consumption of contaminated food, particularly those of animal origin, especially chicken meat. This thesis was fractionated into three chapters, the first one relating to general considerations about the topics discussed in the following chapters. The second chapter aimed to evaluate virulence characteristics, antimicrobial resistance and the genetic similarity of 51 strains of S. Infantis isolated in samples of poultry origin from an industry located in the state of São Paulo, Brazil, during the 2009 to 2010 period. The third chapter aimed to analyze 111 strains of S. Enteritidis, 45 of Salmonella Typhimurium and 31 of Salmonella Typhimurium monophasic variant I 4, [5], 12:i:- isolated from chicken carcasses in different brazilian slaughterhouses from 2009 to 2011, and to estimate the risk to human health, based on the presence of virulence genes and antimicrobial resistance, correlating to the pathogenicity profiles (antimicrobial resistance and presence of virulence and resistance genes) with the genetic profile (ribogroup) of the isolates. To evaluate the antimicrobial susceptibility was performed the disk diffusion test for all serotypes of Salmonella, and exclusively to S. Enteritidis and S. Typhimurium, was also verified the minimum inhibitory concentration for ciprofloxacin and ceftazidime antibiotics. The presence of virulence genes invA (invasion), lpfA (fimbriae-adhesion), agfA (fimbriae-biofilm) and sefA (fimbriae-adhesion) were evaluated by PCR. The strains that showed resistance to antibiotics of β-lactams class were evaluated for the presence of resistance genes blaTEM, blaSHV, blaCTX-M and blaAmpC. For resistant strains to quinolones and fluoroquinolones antibiotics classes were searched the qnrA and qnrS genes. The phylogenetic relationship among the isolates was determined by RAPD method for S. Infantis strains, and by ribotyping technique to S. Enteritidis and S. Typhimurium.

Aplicación móvil para la enseñanza de genética

El presente trabajo tiene su origen en la necesidad de herramientas de apoyo al aprendizaje para los alumnos en las clases de Genética de la Facultad de Biología de la Universidad Complutense de Madrid. En esta asignatura, el equipo docente ha desarrollado aplicaciones para dispositivos móviles destinadas a los alumnos. Las aplicaciones les permiten trabajar con materiales relacionados con aspectos clave de la asignatura. Estas aplicaciones contienen apartados de teoría y ejercicios. Los ejercicios cuentan con asistentes automatizados que guían al alumno para su realización y autocorrección. En su forma actual, las aplicaciones presentan limitaciones tanto desde el punto de vista de su diseño como de la funcionalidad que ofrecen. El actual diseño no aplica las técnicas comunes de Ingeniería del Software respecto a aplicaciones cliente-servidor. Ello las hace difíciles de mantener cuando se plantea abordar nuevas funcionalidades y plataformas, o facilitar la creación de nuevos materiales de la asignatura. Ello ha limitado su expansión para incorporar nuevos tipos de materiales (en particular diferentes tipos de ejercicios), integrarlas con otras herramientas (por ejemplo, el Campus Virtual de la universidad) o permitir un apoyo efectivo a la comunidad de aprendizaje formada por alumnos y docentes (por ejemplo, para que los docentes supervisen la evolución de los alumnos y estos puedan obtener información adicional de los profesores). Para abordar esta situación se propone una aplicación móvil que engobe a todas las aplicaciones anteriores que se habían creado para las clases de Genética. Se utilizará un modelo cliente-servidor para mejorar sus capacidades funcionales, de modo que cumpla con los requisitos establecidos. Entre estos se incluye un control de los usuarios que utilizan la aplicación, y que se optimice la memoria local utilizada por la aplicación, permitiendo así el uso de imágenes más pesadas. Además, este modelo facilitará las tareas de mantenimiento de la aplicación, por ejemplo incluir nuevo material. Por otro lado, también se propone rediseñar la interfaz de la aplicación, de modo que sea más accesible desde el punto de vista de la usabilidad.

Influencia genética de las proteínas surfactantes SP-A y SP-D en la neumonía, la gripe por A (H1N1) 2009 y el asma alérgico

Programa de doctorado: Salud pública: Epidemiología, nutrición y planificación

Diversidad genética y estructura poblacional de caballa ("Scomber colias", Gmelin, 1789) en aguas del Atlántico y del Mediterráneo

Programa de doctorado: Oceanografía, Bienio 2007/09. La fecha de publicación es la fecha de lectura