Potencial antifúngico, antioxidante e inibidor da produção de aflatoxina por extratos fenólicos de Chlorella sp. e Spirulina platensis

A contaminação fúngica acarreta alterações na qualidade nutricional e no valor econômico de produtos alimentícios podendo causar danos patológicos em plantas, animais e humanos. A identificação da atividade antioxidante, antifúngica e antimicotoxinas, em extratos de microalgas com propriedade de inibir a multiplicação de fungos e subseqüente produção de micotoxinas abre a perspectiva de empregar substâncias mais eficientes e com maior ação específica contra estes microorganismos. Entre os compostos com propriedades inibidoras de radicais livres, de crescimento fúngico e produção de micotoxinas, destacam-se os compostos fenólicos, que podem inibir a atividade metabólica microbiana, dificultando a atividade de enzimas. Neste estudo foram avaliados o poder de inibição de multiplicação fúngica de Rhizopus oryzae e Aspergillus flavus pelos extratos fenólicos de Chlorella sp. e Spirulina platensis, bem como sua atividade antioxidante, e a atividade antimicotoxinas da última microalga contra Aspergillus flavus. O conteúdo de fenóis totais foi em média 1000 µgfenóis/g Spirulina platensis e 600 µgfenóis/g Chlorella sp., sendo que o acido gálico e o cafeíco foram identificados como compostos majoritários na Spirulina platensis. As determinações de glicosamina (parede celular) e ergosterol (membrana celular) mostraram-se bons indicativos do desenvolvimento microbiano permitindo uma boa estimativa da inibição dele. O extrato fenólico de Spirulina platensis apresentou capacidade de inibir cerca de 50% a formação da parede e da membrana celular para ambos os fungos estudados e de 100% a produção de aflatoxina B1 até o 10º dia de cultivo do Aspergillus flavus. Além disso, o extrato metanólico de Spirulina platensis inativou 53,5% o DPPH reativo, limitou o escurecimento enzimático ocasionado pela peroxidase em 55% e inibiu a peroxidação lipídica em 46% após 14 dias de armazenamento sob luz. Estes resultados mostram que a ação antifúngica, antimicotoxinas e antioxidante está naturalmente presente em alguns tecidos microbianos e que encontrar a forma de extraí-los e aplicá-los como conservantes alimentícios é muito promissor para substituição aos antifúngicos e outros conservantes químicos.

Entre a pesca e a escola: a educação dos povos tradicionais a partir da comunidade pesqueira na ilha da Torotama (Rio Grande/RS)

O presente estudo aborda a temática da educação escolar nas comunidades tradicionais. Essa abordagem ocorre a partir do estudo de caso da Ilha da Torotama, Rio Grande/RS. É reconhecido que as comunidades tradicionais sofrem uma intensa transformação a partir da lógica moderna do capital, no caso do município de Rio Grande, percebe-se o quanto os discursos em torno do Polo Naval, paralelamente com a crise da pesca artesanal, impulsionam o êxodo das comunidades pesqueiras que constituem a região, levando o pescador ao abandono do trabalho, bem como a uma drástica ruptura frente as formas de vida no espaço tradicional de pesca. A partir desse panorama cabe questionar os sentidos que a escolarização assume nesses espaços, portanto: Quais as contradições e possibilidades da educação escolar nas comunidades tradicionais? Além disso, é necessário questionar: É possível (re)pensar as formas de contemplar os anseios desses povos tradicionais? Nesse sentido, busca-se compreender e problematizar as contradições e possibilidades em torno da escolarização em um contexto do Campo, constituído por povos tradicionais. Para o enfrentamento da problemática, assume-se uma postura dialética a partir de Gadotti (2012) e Severino (2001); esse embasamento epistemológico sustenta o olhar e a escuta da pesquisadora frente aos processos presentes no estudo. Com efeito, por meio da utilização da História Oral na perspectiva assumida por Thompson (1992), encontra-se para maior organização do processo de construção dos dados, a História Oral Temática (MEIHY e HOLANDA, 2010) e (MEIHY, 1996). Nesse horizonte, realizam-se entrevistas com três educadores e quatro educandos do Projeto Educação para Pescadores, o qual ocorre enquanto um processo de escolarização na Ilha da Torotama. O estudo aponta para a necessidade de pensar a escola nas comunidades tradicionais a partir do horizonte da Educação do Campo. Essa compreensão ocorre a partir dos desafios encontrados com relação a escolarização das referidas comunidades; haja vista que o Estado não assume de forma efetiva a educação básica nesses contextos, tampouco aborda a possibilidade de retorno dos sujeitos que tiveram a escolarização negligenciada. A omissão do poder público frente as demandas da comunidade, junto as formas de incentivo e beneficiamento da pequena burguesia industrial pesqueira, por exemplo, são indicativos de que as comunidades tradicionais sofrem em seu contexto uma forte contradição, pois, muito embora o Estado tenha enquanto obrigação resguardar tais povos, as demandas do mercado prevalecem na dinâmica evidenciada. Assim, assumir as lutas lançadas pela 8 perspectiva da Educação do Campo, é uma pertinente possibilidade de ruptura com o sentido que a escola vem apresentando nesses espaços: a escola é vista como uma forma de saída da comunidade e do trabalho da pesca. Portanto, acredita-se que ao assumir o horizonte da Educação do Campo, o trabalho desses sujeitos pertencentes as comunidades tradicionais possa ser problematizado, fomentando a construção crítica frente aos desafios impostos pela lógica opressora.

Evaluating consumer price perception: a mental accounting and frame dependent perspective

In this thesis, we evaluate consumer purchase behaviour from the perspective of heuristic decision making. Heuristic decision processes are quick and easy mental shortcuts, adopted by individuals to reduce the amount of time spent in decision making. In particular, we examine those heuristics which are caused by framing – prospect theory and mental accounting, and examine these within price related decision scenarios. The impact of price framing on consumer behaviour has been studied under the broad umbrella of reference price, which suggests that decision makers use reference points as standards of comparison when making a purchase decision. We investigate four reference points - a retailer's past prices, a competitor's current prices, a competitor's past prices, and consumers' expectation of immediate future price changes, to further our understanding of the impact of price framing on mental accounting, and in turn, contribute to the growing body of reference price literature in Marketing research. We carry out experiments in which levels of price frame and monetary outcomes are manipulated in repeated measures analysis of variance (ANOVA). Our results show that where these reference points are clearly specified in decision problems, price framing significantly affects consumers' perceptions of monetary gains derived through discounts, and leads to reversals in consumer preferences. We also found that monetary losses were not sensitive to price frame manipulations.

Alzheimer’s electroencephalogram event scalp and source localization

Alzheimer’s disease is the most common cause of dementia which causes a progressive and irreversible impairment of several cognitive functions. The aging population has been increasing significantly in recent decades and this disease affects mainly the elderly. Its diagnostic accuracy is relatively low and there is not a biomarker able to detect AD without invasive tests. Despite the progress in better understanding the disease there remains no prospect of cure at least in the near future. The electroencephalogram (EEG) test is a widely available technology in clinical settings. It may help diagnosis of brain disorders, once it can be used in patients who have cognitive impairment involving a general decrease in overall brain function or in patients with a located deficit. This study is a new approach to improve the scalp localization and the detection of brain anomalies (EEG temporal events) sources associated with AD by using the EEG.

Entre a teoria e a prática: por uma reflexão sobre o que e como os estudantes de graduação compreendem os processos revolucionários ocorridos na Inglaterra do século XVII

Jörn Rüsen influenciou teoricamente a base deste Trabalho de Conclusão de Mestrado, que se propõe apresentar a consciência histórica como substanciação da forma dos seres humanos se corresponderem ao mundo através da relação temporal entre o presente e o passado para prospectar o futuro, sendo tais condições extremamente necessárias à práxis vital. Através disso, o presente trabalho sofreu algumas alterações, pois passou-se a entender que não era relevante analisar o que apresentavam os livros didáticos por si só, mas sim como as pessoas que os liam se orientavam temporalmente através das informações que eles apresentavam. A partir de então, começou-se a refletir algumas questões para a produção desse trabalho, como, por exemplo: 1) Qual seria o público-alvo da pesquisa?; 2) Qual a justificativa para o público-alvo?; 3) Que metodologia usar para extrair as fontes narrativas?; 4) Que materiais utilizar como base informativa ao público-alvo; 5) Como criar um mecanismo coerente para extrair a percepção temporal e significativa do públicoalvo? 6) Quais as significâncias que o público-alvo apresentou sobre o assunto? Deve-se ficar claro que o assunto permaneceu, que é a análise sobre as modificações políticas, intelectuais, religiosas, econômicas, etc., da Inglaterra durante o século XVII, o que mudou foi somente a forma de análise sobre tal assunto.

Imagens do terceiro setor: um estudo com pais e responsáveis financeiros das organizações educacionais

The present report is the result of an applied research in the educational entities of the third sector, aiming to demonstrate whether the financial influences the perception of users on the image of those entities. For both used the prospect of integrative marketing relationship adapting to and developing a set of indicators which bore the measurement of images from the model of Machado et al (2005) and Kotler and Fox (1994). The sample included a total of 187 parents and financial responsibility in 03 (three) institutions of education in Natal / RN. These data were processed by multivariate statistical analysis, factor analysis, linear regression, analysis of cluster and discriminant analysis. The factor analysis also identified 6 images perceived by users of services. Next were the relationships of cause and effect between the financial and images formed. In discriminant analysis, was identified two distinct groups of parents and guardians with financial perceptions similar and well defined. The result of the work shows that the differential level of financial participation of parents and guardians not influence the formation of the images formed from educational institutions of the third sector

Simulation tools for the analysis of single electronic systems

Developments in theory and experiment have raised the prospect of an electronic technology based on the discrete nature of electron tunnelling through a potential barrier. This thesis deals with novel design and analysis tools developed to study such systems. Possible devices include those constructed from ultrasmall normal tunnelling junctions. These exhibit charging effects including the Coulomb blockade and correlated electron tunnelling. They allow transistor-like control of the transfer of single carriers, and present the prospect of digital systems operating at the information theoretic limit. As such, they are often referred to as single electronic devices. Single electronic devices exhibit self quantising logic and good structural tolerance. Their speed, immunity to thermal noise, and operating voltage all scale beneficially with junction capacitance. For ultrasmall junctions the possibility of room temperature operation at sub picosecond timescales seems feasible. However, they are sensitive to external charge; whether from trapping-detrapping events, externally gated potentials, or system cross-talk. Quantum effects such as charge macroscopic quantum tunnelling may degrade performance. Finally, any practical system will be complex and spatially extended (amplifying the above problems), and prone to fabrication imperfection. This summarises why new design and analysis tools are required. Simulation tools are developed, concentrating on the basic building blocks of single electronic systems; the tunnelling junction array and gated turnstile device. Three main points are considered: the best method of estimating capacitance values from physical system geometry; the mathematical model which should represent electron tunnelling based on this data; application of this model to the investigation of single electronic systems. (DXN004909)

Redes interorganizacionais e inovação: o caso do pólo joalheiro de Belém

This work is a case study based on Belém Jewelry Pole, whose main issue is to understand how the social network (which the Pole is inserted) influences on innovation process on this area. The main objective is to analyze how interorganizational networks impacted/impact on the potential for innovation, creating both limits and opportunities for the companies development. The adopted method analyzed the historical jewelry industry trajectory since the beginning of mineral extraction in the city of Itaituba (in the Pará State) until nowadays. Primary and secondary data were used allowing the view of the dynamics of the network during transformation periods of the main involved actors in the process. The prospect of embeddedness structural as analysis technique allowed verifying the quality of interactors ties, as well as the visualization of their structures. During the jewelry industry trajectory was verified a change in the quality of social relations, modifying the information flow, trust and associations of various links in the production chain. Both direct and indirect ties facilitated the access to remote networks entering new information related to new products, processes and market aspects. This interaction has led to raising the innovation potential causing a qualitative and quantitative improvement in competitiveness of organizations. Some embedded ties allowed the formation of partnerships bringing various economic earnings for those involved in the relationship. Thus, it is understood how aspects related to the position, architecture and quality of ties in a wide social network influenced on the innovation process and eventual jewelry industry trajectory

Rationing medical education.

The purpose of this paper is to discuss the role of rationing in medical education. Medical education is expensive and there is a limit to that which governments, funders or individuals can spend on it. Rationing involves the allocation of resources that are limited. This paper discussed the pros and cons of the application of rationing to medical education and the different forms of rationing that could be applied. Even though some stakeholders in medical education might be taken aback at the prospect of rationing, the truth is that rationing has always occurred in one form or another in medical education and in healthcare more broadly. Different types of rationing exist in healthcare professional education. For example rationing may be implicit or explicit or may be based on macro-allocation or micro-allocation decisions. Funding can be distributed equally among learners, or according to the needs of individual learners, or to ensure that overall usefulness is maximised. One final option is to allow the market to operate freely and to decide in that way. These principles of rationing can apply to individual learners or to institutions or departments or learning modes. Rationing is occurring in medical education, even though it might be implicit. It is worth giving consideration to methods of rationing and to make thinking about rationing more explicit.

Mapeamento cromossômico de genes ribossomais em espécies estuarinas da família Gerreidae Identificação de uniformidade cariotípica e sua empregabilidade com fins biotecnológicos - REPROGEN

Perciformes are dominant in the marine environment, characterized as the largest and most diverse fish group. Some families, as Gerreidae, popularly known as silver jennies, carapebas, or mojarras have a high economic potential to marine fish farming, natural explotation and game fishing. Genetic information of these species are of fundamental importance for their management and production. Despite exist over 13,000 marine fish species described, only 2% were cytogenetically analyzed and less than 1% have some reproductive characteristics known. Induced breeding, cytogenetic characterization and cryopreservation of gametes, represent important areas in applied fish studies. In this project cytogenetic analyzes were performed to acess genetic aspects of Gerreidae species, distributed in coastal and estuarine regions of Northeast Brazil. Different methods for identifying chromosomal regions were employed using conventional techniques (Ag-NORs, C-banding), staining with base-specific fluorochromes (DAPI-CMA3), and physical mapping of ribosomal genes 18S and 5S rDNA, through hybridization in situ with fluorescent probes (FISH). The six species analyzed showed remarkable chromosome conservatism. The 18S and 5S ribosomal genes when analyzed in phylogenetic perspective demonstrate varied evolutionary dynamics, suggesting ocurrence of stasis process in some groups and greater dynamism in others. Double FISH with 18S and 5S probes showed both how efficient cytotaxonomic markers in the homogeneous karyotypes of this group of species. The karyotypic pattern identified in addition to the evolutionary aspects of karyotype, are suggestive of existence of low potential of post-zygotic barrier, prompting further research to prospect for artificial interspecific hybridization of these species of commercial importance

Attitudes towards risk in financial decision making

Doutoramento em Gestão

Dinâmica do desflorestamento e uso das terras na região dos formadores do rio Xingu.

Because of the occupation occurred in the last thirty years at Xingu river basin, this region has been suffering a large deforestation pressure, especially on its headwaters areas. This study aims to apply GIS techniques to evaluate how land use change has influenced the deforestation dynamics of Xingu water basin in Mato Grosso State. For that, a GIS based study was carried out were the deforestation data for the period between 2000 and 2005 was spatially integrated with settlement areas, indigenous lands, sites of mineral deposits and prospect areas. From this spatially integration, it was possible to analyze statistically how the deforestation has manifested on each kind of occupation, considering the original forest area. The techniques used, including inventory and database organization on GIS environment, and spatial analysis tools made it possible to analyze the deforestation in the Xingu basin in Mato Grosso State between the period of 2000 and 2005, and identify the most affected areas, considering different land uses.

Transição de fase topológica e transporte eletrônica em grafenos e nanofitas zigzag grafeno tensionados

Tese (doutorado)—Universidade de Brasília, Instituto de Física, 2015.

Europeanization beyond the EU: The case of Georgia.

320 p.

Estudo de massa cerâmica triaxial visando à otimização do ciclo de queima da indústria de cerâmica estrutural

Over recent years the structural ceramics industry in Brazil has found a very favorable market for growth. However, difficulties related to productivity and product quality are partially inhibiting this possible growth. An alternative for trying to solve these problems and, thus, provide the pottery industry the feasibility of full development, is the substitution of firewood used in the burning process by natural gas. In order to contribute to this process of technological innovation, this paper studies the effect of co-use of ceramic phyllite and kaolin waste on the properties of a clay matrix, verifying the possible benefits that these raw materials can give to the final product, as well as the possibility of such materials to reduce the heat load necessary to obtain products with equal or superior quality. The study was divided into two steps: characterization of materials and study of formulations. Two clays, a phyllite and a residue of kaolin were characterized by the following techniques: laser granulometry, plasticity index by Atterberg limits, X-ray fluorescence, X-ray diffraction, mineralogical composition by Rietveld, thermogravimetric and differential thermal analysis. To study the formulations, specifically for evaluation of technological properties of the parts, was performed an experimental model that combined planning involving a mixture of three components (standard mass x phyllite x kaolin waste) and a 23 factorial design with central point associated with thermal processing parameters. The experiment was performed with restricted strip-plot randomization. In total, 13 compositional points were investigated within the following constraints: phyllite ≤ 20% by weight, kaolin waste ≤ 40% by weight, and standard mass ≥ 60% by weight. The thermal parameters were used at the following levels: 750 and 950 °C to the firing temperature, 5 and 15 °C/min at the heating rate, 15 and 45min to the baseline. The results showed that the introduction of phyllite and/or kaolin waste in ceramic body produced a number of benefits in properties of the final product, such as: decreased absorption of water, apparent porosity and linear retraction at burn; besides the increase in apparent specific mass and mechanical properties of parts. The best results were obtained in the compositional points where the sum of the levels of kaolin waste and phyllite was maximal (40% by weight), as well as conditions which were used in firing temperatures of 950 °C. Regarding the prospect of savings in heat energy required to form the desired microstructure, the phyllite and the residue of kaolin, for having small particle sizes and constitutions mineralogical phases with the presence of fluxes, contributed to the optimization of the firing cycle.