Biotecnologias aplicadas à reprodução de éguas velhas

The acceptance of biotechnology for the most equine breeders association had a significant effect in the horse industry, gaining popularity around the world, because the increasing on the genetic gain, allowing the use of sub fertile mares and stallions with high genetics value on reproduction. The embryos in vitro production of human and cattle has been used with success, however in vitro embryo production is not efficient in the horse, as oocyte transfer (OT) and intracytoplasmatic sperm injection (ICSI). The oocyte transfer has been used especially in subfertile old mares presenting reproductive pathologies as: endometrite, cervical and uterine adhesions, blocked oviduct, perineal laceration and ovulation failures. During oocyte recovery process, the oocytes must be collected from immature follicles that need be matured in vitro or in vivo matured oocytes from pre-ovulatory follicles through the transvaginal aspiration guided by ultrasound. The recovered oocyte is transferred to a previously inseminated recipient mare, through the flank laparotomy. The intracytoplasmatic sperm injection (ICSI) is a procedure of in vitro fertilization that needs only one sperm that is aspirated and injected inside the oocyte. The oocytes used, can be from mature and immature follicles. Fresh, cooled and frozen semen can be used, because the procedure not requires a functional sperm. The use of Piezo drill resulted in a breakthrough the pellucid zone, allowing the vibration per minute provided in the sperm injection pipette, a major result of cleaved oocytes, due to a better sperm injection in the oocyte. The embryo transfer can be straight inside the oviduct, as also transcervical transferred after embryo culture produced in vitro. In conclusion both procedures (OT and ICSI) are effective to be used on equine assisted reproduction, getting results even lower than expected, but satisfactory from animal genetically superior

Educational and professional background and success in the labor market: the case of Portuguese immigrants in the Netherlands

The aim of this paper is to describe the relationship between educational and professional occupation trajectories of Portuguese immigrants in The Netherlands. To this end, the sample used comprises Portuguese immigrants that arrived in The Netherlands in two periods which represent the highest influx of immigrants: around the years 1966 and 2002. The analysis of the data shows a strong relationship between the level of professional education and the level of professional occupation. Furthermore, some exceptions to the generalizations found show that in service training may lead to an improvement of the possibilities to ascend regarding the level of professional occupation.

“Eco-innovations in more sustainable supply chains for a low-carbon economy: A multiple case study of human critical success factors in Brazilian leading companies”

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Manifestation of estrous behavior and subsequent progesterone concentration at timed-embryo transfer in cattle are positively associated with pregnancy success of recipients

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Talent detection in taekwondo: which factors are associated with the longitudinal competitive success?

Background Several morphological and functional characteristics are associated with the performance of taekwondo (TKD) adult athletes. However, we did not find any longitudinal study associating these features to the future performance of young athletes, and thereby, identifying the best variables to use in a battery of tests to talent detection. Therefore, the aim of this study is answer the question which factors are associated with the longitudinal competitive success of TKD young athletes over five competitive years (2008 to 2012).Material &Methods: Six taekwondo athletes (13.06 +/- 1.07 years, 43.6 +/- 6.6 kg, 157.9 +/- 8.3 cm), who trained three to six hours per week, for more than three years, were assessed on 32 maturational indicators, of body composition, anthropometric and functional, using anthropometric techniques, dual energy x-ray absorptiometry, carpal radiography and contact platform. To determine the competitive ranking, the competitive results of athletes from 2008 to 2012 were analysed, and these values were correlated with the other 32 indicators for determining the Longitudinal Predictors of Performance in TKD (LPPT). Moreover, one of the athletes achieved results notably higher than the other, being medalled at Junior World Championships. Therefore, all variables were transformed into z-scores and all those in which this athlete presented superior performance in 1 z-scores were considered as LPPT. Results: The athlete of reference (the first in Longitudinal Competitive Ranking 2008-2012) distinguished, in accordance with the criteria, in nineteen LPPT indicators. The ranking was correlated with 6 LPPT parameters, including one from the maturation group of indicators and five from the functional group.Conclusion: Our results allowed us to identify several factors that are related to longitudinal competitive success in taekwondo young athletes. These factors should be considered by coaches to the proper selection of training programs, as well as for the pre-selection of young talents in competitive taekwondo. However, these results apply only to the Portuguese taekwondo adolescent athletes, being of limited generalizability.

Effect of antioxidants during bovine in vitro fertilization procedures on spermatozoa and embryo development

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Expression of α6 integrin subunit in bovine oocyte and its potential role during fertilization

Fertilization in mammals requires the successful completion of a sequence of steps, starting with the transport of gametes in the reproductive tract and ending with sperm-egg membrane fusion to produce a zygote. Although some integrin subunits are known to be associated with the plasma membrane of some mammalian oocytes and spermatozoa, the presence of α6 integrin on bovine oocytes with intact zona pellucida has not been reported. The present study was undertaken to evaluate the expression of α6 integrin subunit in bovine oocyte and to determine if in vitro binding to the zona pellucida and fertilization were affected by treating oocytes with α6 integrin subunit antibody. The α6 integrin subunit was identified on the bovine oocyte by immunocytochemistry. In vitro fertilization was significantly decreased when in vitro matured bovine oocytes were pre-incubated with α6 integrin subunit antibody at concentration 5 and 20 μg/mL, and spermoocyte binding increased. These studies demonstrated the presence of α6 integrin subunit on bovine oocyte, and its importance in fertilization and polyspermy.

Avaliações fisiológicas de sorgo sacarino inoculado com Azospirillum brasilense em função da adubação nitrogenada e reguladores vegetais

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Soil fertility, nutrition and early growth of physic nut as affected by nitrogen fertilization

The initial growth and mineral nutrition of the physic nut (Jatropha curcas L.) as a function of nitrogen (N) fertilization was investigated. The transplanting of seedlings was carried out in plastic pots filled with 50 dm3 of a Rhodic Hapludox, under a plastic greenhouse. In addition to a control treatment, the dosages of 0, 40, 80, 120, and 160 mg dm−3 N were tested. The results demonstrated that N fertilization for the cultivation of physic nut could be added as top dressing from 60 days after planting with a dosage of 65 mg dm−3. A SPAD index of 46 can be used as a nutritional reference to its initial development. Furthermore, the results suggested that the order of nutrient accumulation by the physic nut plants is as follows: potassium (K) > N > magnesium (Mg) > calcium (Ca) > phosphorus (P) > sulfur (S) > iron (Fe) > manganese (Mn) > boron (B) > zinc (Zn) > copper (Cu).

Measuring Success One Student At A Time

This presentation describes Murray State University's proactive efforts to enhance African¬-American students' preparation, recruitment/retention and graduation. Strategies utilized to create and maintain a positive/hospitable campus environment will be delineated. It is our campus-wide responsibility to "nurture" each student with personalized contact and carefully selected services to engender degree persistence.

The Importance of Community and Its Impact on the Quality of Campus Life and Success for African American Students

“While we are accustomed to viewing special programs as efforts to ensure the success of underrepresented students, we may overlook that what these programs communicate about these students are part of the structure of higher education that they must struggle against.”

LIMB REPLANTATION AFTER AVULSION INJURIES: TECHNIQUES AND TACTICS FOR SUCCESS

Objectives: Retrospective evaluation of cases of limb replantation after avulsion injuries. Evaluation of the techniques and tactics used, that contributed to success and good functional results. Methods: Forty-three patients' records were assessed. All the cases had been submitted to limb replantation after avulsion injuries. Results: The majority of the cases were young men. The most common injury was to the thumbs. The surgical techniques and tactics used were: nerve grafting, vein grafting, transposition of the digital vessels, limb shortening, and heterotopic replantation. The most commonly used technique was vein graft. The limb survival rate was high (93%), as was patient satisfaction. Conclusion: Replantation after avulsion injury depends on the correct diagnosis of the limb viability and the use of appropriate surgical techniques and tactics for each case. The experience of the team of surgeons and a good hospital structure are essential for good results. There are few articles in medical literature about the indications, techniques and results of limb replantation after avulsion injuries. We believe that this retrospective evaluation can bring new information and contributions to the correct management of this highly complex situation. Level of evidence IV, Case Series.

Strategies in identifying individuals in a segregant population of common bean and implications of genotype x environment interaction in the success of selection

The objective of this study was to compare the BLUP selection method with different selection strategies in F-2:4 and assess the efficiency of this method on the early choice of the best common bean (Phaseolus vulgaris) lines. Fifty-one F-2:4 progenies were produced from a cross between the CVIII8511 x RP-26 lines. A randomized block design was used with 20 replications and one-plant field plots. Character data on plant architecture and grain yield were obtained and then the sum of the standardized variables was estimated for simultaneous selection of both traits. Analysis was carried out by mixed models (BLUP) and the least squares method to compare different selection strategies, like mass selection, stratified mass selection and between and within progeny selection. The progenies selected by BLUP were assessed in advanced generations, always selecting the greatest and smallest sum of the standardized variables. Analyses by the least squares method and BLUP procedure ranked the progenies in the same way. The coincidence of the individuals identified by BLUP and between and within progeny selection was high and of the greatest magnitude when BLUP was compared with mass selection. Although BLUP is the best estimator of genotypic value, its efficiency in the response to long term selection is not different from any of the other methods, because it is also unable to predict the future effect of the progenies x environments interaction. It was inferred that selection success will always depend on the most accurate possible progeny assessment and using alternatives to reduce the progenies x environments interaction effect.