Effect of antioxidants during bovine in vitro fertilization procedures on spermatozoa and embryo development


Autoria(s): Gonçalves, Fernanda da Silva; Barreto, Letícia Siqueira de Sá; Arruda, Rubens Paes de; Perri, Silvia Helena Venturoli; Mingoti, Gisele Zoccal
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

28/01/2016

28/01/2016

2010

Resumo

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Increased amounts of reactive oxygen species (ROS) duringin vitro fertilization (IVF) may cause cytotoxic damage togametes, whereas small amounts of ROS favour spermcapacitation. The aim of this study was to investigate theeffect of antioxidants [50 lM b-mercaptoethanol (b-ME) and50 lM cysteamine (Cyst)] or a pro-oxidant (5 mM buthioninesulfoximine) on the quality and penetrability of spermatozoainto bovine oocytes and on the subsequent embryo develop-ment and quality when added during IVF. Sperm quality,evaluated by the integrity of plasma and acrosomal mem-branes, and mitochondrial function, was diminished (p < 0.05) after 4-h culture in the presence of antioxidants.Oocyte penetration rates were similar between treatments (p > 0.05), but antioxidants adversely affected the normalpronuclear formation rates (p < 0.05). The incidence ofpolyspermy was high for b-ME (p < 0.05). No differenceswere observed in cleavage rates between treatments (p > 0.05). However, the developmental rate to the blasto-cyst stage was adversely affected by Cyst treatment (p < 0.05). The quality of embryos that reached the blasto-cyst stage, evaluated by total, inner cell mass (ICM) andtrophectoderm cell numbers and ICM/total cell ratio wasunaffected (p > 0.05) by treatments. The results indicate that ROS play a role in the fertilizing capacity in bovinespermatozoa, as well as in the interaction between thespermatozoa and the oocytes. It can be concluded thatsupplementation with antioxidants during IVF proceduresimpairs sperm quality, normal pronuclear formation andembryo development to the blastocyst stage.

Formato

129-135

Identificador

http://dx.doi.org/10.1111/j.1439-0531.2008.01272.x

Reproduction in Domestic Animals, v. 45, n. 1, p. 129-135, 2010.

0936-6768

http://hdl.handle.net/11449/133634

10.1111/j.1439-0531.2008.01272.x

0810837598202634

7673684272089087

Idioma(s)

eng

Publicador

Blackwell Verlag

Relação

Reproduction in Domestic Animals

Direitos

closedAccess

Tipo

info:eu-repo/semantics/article