851 resultados para CONGELACIÓN DE SEMEN
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Literaturverz. S. 587 - 596
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Literaturverz. S. 484 - 496
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S. 341 - 527 fehlen: werden nachgescannt 10.11.14
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von S. M. Dubnow
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von S. M. Dubnow. Autoris. Übers. aus dem Russ. von I. Friedländer
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by S. M. Dubnow. Transl. from the Russian by I. Friedlaender
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von S. M. Dubnow. Autoris. Übers. aus d. Russ. von Israel Friedländer
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The goal of this work was the development of suitable (real-time) RT-PCR techniques for fast and sensitive diagnosis of EAV and for molecular-epidemiological characterisation of viral strains, as an alternative to virus isolation. To this purpose two conventional RT-PCR methods and one real-time RT-PCR were adapted to detect the broadest possible spectrum of viral strains. Several dilutions with Bucyrus strain showed a 100-fold higher sensitivity of real-time RT-PCR and heminested RT-PCR compared to simple RT-PCR. Making use of 11 cell culture supernatants of different EAV isolates and 7 semen samples of positive stallions, the suitability of the techniques could be shown. Phylogenetic analysis of sequences of the newly analysed samples compared with known sequences indicated that more EAV-lineages exist than presently described.