Electrochemiluminescence detection of NADH and ethanol based on partial sulfonation of sol-gel network with gold nanoparticles

We developed a stable, sensitive electrochemiluminescence (ECL) biosensor based on the synthesis of a new sol-gel material with the ion-exchange capacity sol-gel to coimmobilize the Ru(bpy)(3)(2+) and enzyme. The partial sulfonated (3-mercaptopropyl)-trimethoxysilane sol-gel (PSSG) film acted as both an ion exchanger for the immobilization of Ru(bpy)(3)(2+) and a matrix to immobilize gold nanoparticles (AuNPs). The AuNPs/PSSG/Ru(bpy)(3)(2+) film modified electrode allowed sensitive the ECL detection of NADH as low as 1 nM. Such an ability of AuNPs/PSSG/Ru(bpy)(3)(2+) film to promote the electron transfer between Ru(bpy)(3)(2+) and the electrode suggested a new, promising biocompatible platform for the development of dehydrogenase-based ECL biosensors. With alcohol dehydrogenase (ADH) as a model, we then constructed an ethanol biosensor, which had a linear range of 5 mu M to 5.2 mM with a detection limit of 12 nM.

High-sensitivity determination of lead and cadmium based on the Nafion-graphene composite film

Graphene nanosheets, dispersed in Nafion (Nafion-G) solution, were used in combination with in situ plated bismuth film electrode for fabricating the enhanced electrochemical sensing platform to determine the lead (Pb2+) and cadmium (Cd2+) by differential pulse anodic stripping voltammetry (DPASV). The electrochemical properties of the composite film modified glassy carbon electrode were investigated. It is found that the prepared Nafion-G composite film not only exhibited improved sensitivity for the metal ion detections, but also alleviated the interferences due to the synergistic effect of graphene nanosheets and Nafion. The linear calibration curves ranged from 0.5 mu g L-1 to 50 mu g L-1 for Pb2+ and 1.5 mu g L-1 to 30 mu g L-1 for Cd2+. respectively. The detection limits (S/N = 3) were estimated to be around 0.02 mu g L-1 for Pb2+ and Cd2+. The practical application of the proposed method was verified in the water sample determination.

Sensitive biomimetic sensor based on molecular imprinting at functionalized indium tin oxide electrodes

We initially report an electrochemical sensing platform based on molecularly imprinted polymers (MIPs) at functionalized Indium Tin Oxide Electrodes (ITO). In this research, aminopropyl-derivatized organosilane aminopropyltriethoxysilane (APTES), which plays the role of functional monomers for template recognition, was firstly self-assembled on an ITO electrode and then dopamine-imprinted sol was spin-coated on the modified surface. APTES which can interact with template dopamine (DA) through hydrogen bonds brought more binding sites located closely to the surface of the ITO electrode, thus made the prepared sensor more sensitive for DA detection. Potential scanning is presented to extract DA from the modified film, thus DA can rapidly and completely leach out. The affinity and selectivity of the resulting biomimetic sensor were characterized using cyclic voltammetry (CV). It exhibited an increased affinity for DA over that of structurally related molecules, the anodic current for DA oxidation depended on the concentration of DA in the linear range from 2 x 10(-6) M to 0.8 x 10(-3) M with a correlation coefficient of 0.9927.In contrast, DA-templated film prepared under identical conditions on a bare ITO showed obviously lower response toward dopamine in solution.

Liquid crystalline polymer as a processing agent in blends of poly(ether-ketone)/LCP70

Blends of a liquid crystalline thermotropic copolyester (LCP70) and an amorphous phenolphthalein based poly(ether-ketone)(PEK-C) with two viscosities were prepared by melt blending. The blends' morphology, rheological and mechanical properties were investigated by DSC, SEM, mechanical and rheological tests. It was observed that the optimum composition of the PEK-C/LCP70 blend was 10 wt% LCP for both mechanical and rheological properties. When the LCP content was less than 10%, the LCP phase existed as finely dispersed fibrous domains with a diameter of about 1 mu m in the matrix, and both tensile and flexural properties were improved. In contrast, when the LCP content reached 20% or more, the LCP domains coalesced to ellipsoidal particles with a diameter of about 5 mu m, and the mechanical properties decreased as a result. It is demonstrated that pure PEK-C with a high viscosity which was difficult to process by melt extrusion, could be extruded conveniently when 10% LCP70 was incorporated. It is emphasized that LCP not only can be used as a reinforcing phase but also an effective processing agent for engineering thermoplastics, especially for those with high viscosity and narrow processing window. (C) 1997 Elsevier Science Ltd.

基于粗糙集和多Agent系统的知识挖掘

通过优化知识表达系统中条件属性对决策属性的依赖度,深入研究了粗糙集并与多Agent系统相结合。利用离散粒子群算法,提出一种基于粒子群优化的粗糙集知识约简算法,该算法解决了启发式算法无法全局搜索进行约简的问题。最后通过在矿井中调度信息的应用验证了有效性。

BDI Agent的人机智能结合方法

在网络遥操作中存在着由于操作者的操作负担过重或长时间操作而引起的误操作问题,提出以著名的BDIAgent模型为基础,通过引入中断机制,将反映操作者智能决策的操作目标、操作意图与BDIAgent推理产生的目标、意图进行结合的方法,为解决Agent智能的局限性和操作者的误操作问题,提供了一种人机接口方法,实现了人机智能结合.给出了中断的引入方法和操作者操作目标、操作意图中断响应原理,用形式化模型描述了人机智能决策选取目标和意图的过程.该人机智能结合方法在网络遥操作RoboCup中型足球机器人系统中得到了应用.

基于Agent的网络遥操作机器人系统控制研究

为了充分发挥智能机器人的自主性和人的主观能动性,实现通过网络遥操作非结构化环境下的智能机器人,提出了一种基于Agent的网络遥操作机器人控制系统结构,分析了控制系统中各部分的功能,给出了系统中各个Agent的实现方法。该控制方法已通过仿人形机器人的遥操作控制实验得到了验证。

可重构装配系统中智能体的开发

为了实现可重构装配系统的快速重构能力,在分析了传统控制结构的基础上,结合分层递阶和分布式控制的优点,提出了基于多智能体系统的混合控制体系结构。这种结构具有低复杂性、强鲁棒性、易维护性、可扩展性以及可重用性等特点,能够适应重构系统的要求。在遵守智能物理代理基金会规范的前提下,以Java智能体开发平台进行了装配系统中各层智能体的实例开发。

基于agent及holon的可重构生产线实现技术

以大批量制造的生产线制造系统为背景,研究基于agent与holon智能制造思想体系的可重构生产线制造系统实现技术,以提高生产线柔性与可重构能力为目标·深入分析了多agent制造及holonic制造模式体系下智能单元自主决策、协商合作在制造系统环境中的实现与运行机制,在以机械加工线和装配线为代表的不同的工程实际背景与应用环境下该思想体系目前的具体应用情况与实现技术·指出该思想体系面向具体工程背景的有效实施应用是理论研究的关键·最后以某汽车变速器装配生产线制造系统为应用实例,从其规划设计与运行角度,分析了可重构生产线系统的框架设计及具体实现技术与方法·

基于多Agent可重构机器人控制方法的研究

根据多 Agent理论中的协商、合作机制和可重构机器人结构的分布性 ,将集中式的机器人控制分配到一组关节 Agent中 ,每个 Agent控制机器人的一个关节 ,使用这种分布式方法 ,得到了一种新的通用机器人控制方法 ,即将关节机器人的复杂控制转换为多个简单子系统的控制 ,该方法可应用于具有不同构型的机器人系统 ,特别适用于可重构模块化机器人的控制。利用微分运动理论提出了一种新的决策方法 ,便于 Agent之间的合作与协商。仿真实验结果表明该方法是一种可行的机器人控制方法

基于Agent的工作流协调模型研究

本文分析了工作流管理系统的现状及存在的问题 ,提出了基于 agent的工作流协调管理模型 .该模型在面向对象的工作流元过程模型基础上 ,利用 agent和面向对象技术 ,建立协调 agent、实例化 agent和活动 agent模型 ,提高系统的自组织、自学习和协调能力 .

基于CORBA的多agent供应链管理系统的研究

本文从现代供应链管理概念及要求出发 ,指出协调与合作在供应链管理中的重要作用 ,应用分布式人工智能理论及分析方法 ,提出了基于多 agent的供应链管理结构 ,定义了各功能agent的作用和职能 ,研究了基于多 agent的供应链管理协作内容、特点及解决方法 ,介绍了基于CORBA的分布式系统开发方法 ,提出了基于 CORBA规范及 KQML协议的支持供应链管理协作研究及开发的软件结构 .

一个多智能体机器人协作装配系统

介绍了一个基于多智能体概念实现的多机器人协作装配系统——ＭＲＣＡＳ（Ｍｕｌｔｉ－ＲｏｂｏｔＣｏｏｐｅｒａｔｉｖｅＡｓｓｍｂｌｙＳｙｓｔｅｍ）。该系统由组织级计算机、三台工业机器人和一台全方位移动小车（ＯＤＶ）组成，采用分层递阶体系结构。利用ＭＲＣＡＳ系统进行了多机器人协作装配的实验：在ＯＤＶ装配平台上，四台机器人合作装配一个大型桁架式工件。该工件具有多种装配构型，但任何一台机器人不能独立完成装配。

基于多Agent制造过程的建模方法

分析了制造系统与制造过程之间的关系;论证了从过程的角度对制造进行建模更恰当;结合Agent和π演算的特点,给出Agent制造系统描述模型及基于π演算的单个Agent的BDI模型,并指出Agent和π演算结合的制造过程模型有利于进行优化目标在不同制造过程层次的分解,不论从方法的角度还是实现的角度,都适合复杂系统建模。Agent和π演算相结合可以有效分析并解决离散事件的建模与仿真中的问题。

Robust Agent Control of an Autonomous Robot with Many Sensors and Actuators

This thesis presents methods for implementing robust hexpod locomotion on an autonomous robot with many sensors and actuators. The controller is based on the Subsumption Architecture and is fully distributed over approximately 1500 simple, concurrent processes. The robot, Hannibal, weighs approximately 6 pounds and is equipped with over 100 physical sensors, 19 degrees of freedom, and 8 on board computers. We investigate the following topics in depth: distributed control of a complex robot, insect-inspired locomotion control for gait generation and rough terrain mobility, and fault tolerance. The controller was implemented, debugged, and tested on Hannibal. Through a series of experiments, we examined Hannibal's gait generation, rough terrain locomotion, and fault tolerance performance. These results demonstrate that Hannibal exhibits robust, flexible, real-time locomotion over a variety of terrain and tolerates a multitude of hardware failures.