A preliminary study on the evaluation of casein, shrimp meal, squid meal and spirulina as protein sources of Penaeus monodon (Fabricius) postlarvae

P. monodon postlarvae were fed with fresh brown mussel (Modiolus metcalfei ) meat, and artificial diets containing casein, shrimp (Metapenaeus ensis ) meal, squid (Loligo ) meal, and Spirulina as protein sources at a rate of 20% of their biomass per day for a period of 10 days. No statistical difference was found among the weight gains of shrimp fed on various experimental diets. However, shrimp fed squid meal diet had highest weight gain followed by shrimp meal, fresh brown mussel meat, casein and Spirulina . Shrimp fed squid meal diet had a significantly better feed conversion and those receiving other experimental diets. Poorest fed conversion was observed in shrimp fed Spirulina . The protein efficiency ratio was statistically highest for the squid meal diet, with the lowest value again for Spirulina diets. Survival rate was significantly highest for shrimp fed the shrimp meal diet. No statistical differences were found among shrimp fed other experimental diets. Appropriate data are tabulated.

Growth and survival rates of hatchery-produced and wild milkfish fry grown to fingerling size in earthen nursery ponds

Growth and survival rates of hatchery-produced and wild milkfish (Chanos chanos ) fry grown to fingerling size were compared. Data show no significant difference between the 2 fry. At a recommended stocking density of 30 fry/m super(2), hatchery-produced milkfish fry could attain fingerling size of almost 2g with a survival of 68%. The study indicates that hatchery-produced fry/fingerlings can equal the culture performance of the wild fry. Comparative performance of hatchery-bred and wild fry should encourage intensified research on milkfish broodstock development and refinement of induced spawning methods.

Studies on two unexploited fish species: Peristedion adeni and Peristedion weberi

The chemical composition and nutritional quality of Peristedion adeni and Peristedion weberi, two unexploited fish species available in abundance in the Exclusive Economic Zone of India are reported. Size of the fish is small and separation of edible meat is difficult. Best method of utilization of the fish is conversion into meal. Meals having crude protein content varying from 55.0 to 57.0%, ash content from 17.0 to 19.6% and fat content from 17.7 to 18.1% can be prepared from the two species of fish. PER values of the meals are lower than that of casein. Amino acid analysis showed that isoleucine, leucine and valine are not present in the meals in adequate quantities compared to the FAO/WHO (1973) prescribed pattern.

On the optimum mesh size for the capture of Barbus tor (Hamilton)

Results of mesh selectivity experiments on B. tor are presented in this paper. Selectivity curve on the basis of maximum girth of fish in relation to perimeter of mesh was worked out. The optimum girth/mesh perimeter ratio was found to be 1.31. A linear regression of G+0.445L=12.8 was fitted for conversion of length to girth.

Influences of dietary lipid and phosphorus levels on retention and excretion of phosphorus and nitrogen in fingerlings red sea bream, Pagrus major

A laboratory based 2x3 factorial experiment was conducted for 12 weeks to investigate the influences of dietary lipid and phosphorus (P) levels on retention and excretion of phosphorus and nitrogen (N) in fingerling red sea bream. Two levels of lipid (210 and 260 g/kg) and three levels of phosphorus (17, 14 and 12 g/kgˉ¹) in the dry diets were tested. Duplicate groups of 25 red sea bream (average weight 3.74±0.07 g) per 60L glass tank were fed experimental diets three times a day near to satiation level at 22 to 28°C water temperature. A reduction in dietary fish meal from 500 to 300 g/kg dry diet, corresponding to a supplementation in both dietary lipid and P resulted in significant increase in both P and N retention which resulted in the reduction of their excretion by red sea bream. The overall results of the present study demonstrated that both lipid and phosphorus supplementation are necessary for developing less-polluting feed which in turn, reduce fish meal level in the diet of fingerling red sea bream. Further studies in this regard with different size and age groups of red sea bream are warranted.

The Effects of Visual Object Size in The Depth Perception in KM Mouse

Whether mice perceive the depth of space dependent on the visual size of object targets was explored when visual cues such as perspective and partial occlusion in space were excluded. A mouse was placed on a platform the height of which is adjustable. The platform located inside a box in which all other walls were dark exception its bottom through that light was projected as a sole visual cue. The visual object cue was composed of 4x4 grids to allow a mouse estimating the distance of the platform relative to the grids. Three sizes of grids reduced in a proportion of 2/3 and seven distances with an equal interval between the platform and the grids at the bottom were applied in the experiments. The duration of a mouse staying on the platform at each height was recorded when the different sizes of the grids were presented randomly to test whether the Judgment of the mouse for the depth of the platform from the bottom was affected by the size information of the visual target. The results from all conditions of three object sizes show that time of mice staying on the platform became longer with the increase in height. In distance of 20 similar to 30 cm, the mice did not use the size information of a target to judge the depth, while mainly used the information of binocular disparity. In distance less than 20 cm or more than 30 cm, however, especially in much higher distance 50 cm, 60 cm and 70 cm, the mice were able to use the size information to do so in order to compensate the lack of binocular disparity information from both eyes. Because the mice have only 1/3 of the visual field that is binocular. This behavioral paradigm established in the current study is a useful model and can be applied to the experiments using transgenic mouse as an animal model to investigate the relationships between behaviors and gene functions.

Size distribution of heavy mineral grains in some modern Nile Delta coastal sands, Egypt

This study includes determination and discussion of the texture and heavy mineral compositions of some modem Nile Delta coastal sands (river, coastal dune, beach-face, and nearshore marine) in order to delineate the process and factors that regulate the size distribution of heavy mineral grains comprising these coastal sands. Textural analysis of unseparated bulk samples indicate that the examined four types of sands differ in their mean grain sizes and degree of sorting. However, analysis of size distribution curves of 10 heavy mineral species or group of species in the four environments having the same general shape and nearly similar in that general order of arrangement. However, these curves vary both in median sizes and sorting. The size distribution of a heavy mineral in the Nile Delta coastal sands appear to depend on: (1) range of grain size fractions in each sample, (2) relative availability of heavy mineral in each size grade of the sample, (3) specific gravity of minerals comprising these sands, and (4) some other unknown factor or factors. Results of size measurement of heavy minerals indicated that increasing specific gravity is accompanied by increasing fineness of the heavy minerals. This study may be useful in search for marine placers and understanding the processes of grain-sorting on the sea beaches.

Effect of replacement of fish meal with soybean meal plus supplementary enzymes on growth performance, survival rate and apparent digestibility of rainbow trout (Oncorhynchus mykiss)

This study was carried out to measure the effects of a supplementary multi enzyme on growth performance , survival rate and apparent protein digestibility of rainbow trout fed some diets containing different amounts of soy bean meal. Five exprimental diets with replacement of 25, 50, 75 and 100 percent of fish meal protein by soy bean meal protein were made and 0, 500 and 1000 ppm dosages of supplementary multi enzyme had used in each of them. By the means a diet with fish meal as the only source of protein has used as the control. So this study had 13 treatments. The trouts in 89.40±4.01 gr mean weight were stocked in 39 experimental fiberglass tanks in abundance of 30 fish per any tank. These specimens fed experimental diets for 8 weeks and ten of them in each tank fed same diets which added Cr2O3 to them for one more week to measure the apparent protein digestibility in them. The results shown that supplementary multi enzyme (Avizyme) which contains Protease , Amylase and Xylanase , caused increases in growth performance , survival rate and apparent protein digestibility in trouts which fed soybean meal. Also this study shown that using 1000 ppm of Avizyme in diets which containing soybean meal had the best results and the diet which contained 39 % soybean meal with this amount of enzymes, had no significant differences by the control in any of the studied factors.

Composition and amino acid content of meal from some commercially less important fishes of Maharashtra

Details are given of the yield and composition of dried waste from the filleting wastes of 3 commercially less utilized fish of the Maharashtra coast (Saurida tumbil, Caranx sexfasciatus and Sphyraena jello). The amino acid composition after acid hydrolysis is detailed for the three species.

Photo-assisted hydrosilylation of silicon nanoparticles: Dependence of particle size on grafting chemistry

Silicon nanoparticles between 2.5 nm and 30 nm in diameter were functionalized by means of photoassisted hydrosilylation reactions in the aerosol phase with terminal alkenes of varying chain length. Using infrared spectroscopy and nuclear magnetic resonance, the chemical composition of the alkyl layer was determined for each combination of particle size and alkyl chain length. The spectroscopic techniques were used to determine that smaller particles functionalized with short chains in the aerosol phase tend to attach to the interior (β) alkenyl carbon atom, whereas particles >10 nm in diameter exhibit attachment primarily with the exterior (α) alkenyl carbon atom, regardless of chain length. © 2011 American Chemical Society.

Observations on the influences of codend mesh size on bottom trawl catches in Lake Victoria, with emphasis on the Haplochromis population

Codends of four different mesh size" were compared during exploratory bottom trawling on Lake Victoria. Small mesh sizes (19 and 38 mm) generally caught greater quantities of fish than large mesh sizes (64 and 76 mm) with haplochromis species responsible for the difference. The differences in catch rates were most pronounced where dense concentration of small haplochromis were found. This was generally in shallow water since the average size of haplochromis tends to increase with depth. Catch rates for species other than haplochromis were fairly similar for the codends tested, although there were indications of lower catches in small mesh coderlds fished through dense haplochromis concentrations. For haplochromis fished with 64 and 38 mm eodends, the estimated 50% retention lengths were 13.6 and 8.0 cm, respectively. The predicted value for the 19 mm codend was 4.5 cm.

Possible fish stock size assessment and available production survey as developed on Lake Kariba

This paper is an outline of methods practically useful for the evaluation of ichthyomass, fish abundance, available production and yield in lakes and rivers. Terms and concepts are reviewed, and difficulties stemming from the use of "predetermined" mathematical models are discussed. Sampling with toxicants in blocked-off areas was found to be the most practical method and is described in detail. For the total estimation of ichthyomass the spatial ranges of fish distribution must be determined; the results of echo-sounding surveys for horizontal, vertical, topographical, seasonal and diel fish distribution are given. Some of the most important methods for computing available production are listed and applied to Lake Kariba as an example. In particular, a method based on the balance between the main predator and prey species is reviewed. The ecological production survey concept is finally stressed as applied to multispecies fish stocks.

Techniques to improve the shear strength of impacted bone graft: the effect of particle size and washing of the graft.

BACKGROUND: When fresh morselized graft is compacted, as in impaction bone-grafting for revision hip surgery, fat and marrow fluid is either exuded or trapped in the voids between particles. We hypothesized that the presence of incompressible fluid damps and resists compressive forces during impaction and prevents the graft particles from moving into a closer formation, thus reducing the graft strength. In addition, viscous fluid such as fat may act as an interparticle lubricant, thus reducing the interlocking of the particles. METHODS: We performed mechanical shear testing in the laboratory with use of fresh-frozen human femoral-head allografts that had been passed through different orthopaedic bone mills to produce graft of differing particle-size distributions (grading). RESULTS: After compaction of fresh graft, fat and marrow fluid continued to escape on application of normal loads. Washed graft, however, had little lubricating fluid and better contact between the particles, increasing the shear resistance. On mechanical testing, washed graft was significantly (p < 0.001) more resistant to shearing forces than fresh graft was. This feature was consistent for different bone mills that produced graft of different particle-size distributions and shear strengths. CONCLUSIONS: Removal of fat and marrow fluid from milled human allograft by washing the graft allows the production of stronger compacted graft that is more resistant to shear, which is the usual mode of failure. Further research into the optimum grading of particle sizes from bone mills is required.

Techniques to improve the shear strength of impacted bone graft: The effect of particle size and washing of the graft

Background: When fresh morselized graft is compacted, as in impaction bone-grafting for revision hip surgery, fat and marrow fluid is either exuded or trapped in the voids between particles. We hypothesized that the presence of incompressible fluid damps and resists compressive forces during impaction and prevents the graft particles from moving into a closer formation, thus reducing the graft strength. In addition, viscous fluid such as fat may act as an interparticle lubricant, thus reducing the interlocking of the particles. Methods: We performed mechanical shear testing in the laboratory with use of fresh-frozen human femoral-head allografts that had been passed through different orthopaedic bone mills to produce graft of differing particle-size distributions (grading). Results: After compaction of fresh graft, fat and marrow fluid continued to escape on application of normal loads. Washed graft, however, had little lubricating fluid and better contact between the particles, increasing the shear resistance. On mechanical testing, washed graft was significantly (p < 0.001) more resistant to shearing forces than fresh graft was. This feature was consistent for different bone mills that produced graft of different particle-size distributions and shear strengths. Conclusions: Removal of fat and marrow fluid from milled human allograft by washing the graft allows the production of stronger compacted graft that is more resistant to shear, which is the usual mode of failure. Further research into the optimum grading of particle sizes from bone mills is required. Clinical Relevance: Understanding the mechanical properties of milled human allograft is important when impaction grafting is used for mechanical support. A simple means of improving the mechanical strength of graft produced by currently available bone mills, including an intraoperative washing technique, is described.

Growth rates of Tilapia esculenta (Graham) and Tilapia zillii (Gervais) under cultivation in ponds at Nyegezi, Tanzania

Aquaculture in Tanzania is still on a subsistence level and most of the ponds are maintained as part time job. The ponds are too small, shallow and over crowded with stunted Tilapia spp. In the present paper the results of experiments conducted in ponds at Nyegezi with T. esculenta and T. zillii are presented. This was part of an overall project of developing techniques of fish cultures with Tilapia under the limited existing conditions at Nyegezi. In a mono - species culture experiement with Tilapia zillii in nine month's time an average size of 172.8 mm/115.0 g was attained. In another experiment with T. zillii and T. esculenta in thirteen month's time, T. zillii attained an average size of 180.2mm/106.6 g and T. esculenta 193.6 mm/118.8 g. In another experiment with intensive feeding schedule an average size of 179.3 mm/126.6 g was attained by T. zillii and 191.0 mm/125.0 g by T. esculenta in four month's time. A locally prepared supplimentary feed with local Brewery Waste and Fish Meal (10:1) was readily accepted by both species of Tilapia. T. zillii voraciously fed on Cabbage leaves, Cauliflower leaves, Chinese cabbage leaves, Cassava leaves and on the common weed Comalina sp. Though all the items mentioned above were readily accepted by T. zillii feeding with Comaltna sp. was the easiest and most convenient because of its availability. In an intensive feeding experiment with vegetable leaves/Comalina sp. and the locally prepared supplimentary feed the fishes attained table size in four months time. Cement cistens of 5 X 3 X 1½ m size could be conveniently used for breeding both species of Tilapia. T. zillii had semi adhesive eggs and they were deposited on the sides of the cement wall. The number of young ones in a brood ranged from 160 to 314 in T. esculenta and 687 to 4,356 in T. zillii.