Revisiting the Acanthamoeba species that form star-shaped cysts (genotypes T7, T8, T9, and T17): characterization of seven new Brazilian environmental isolates and phylogenetic inferences

Free-living amoebae of the genus Acanthamoeba are the agents of both opportunistic and non-opportunistic infections and are frequently isolated from the environment. Of the 17 genotypes (T1-T17) identified thus far, 4 (T7, T8, T9, and T17) accommodate the rarely investigated species of morphological group I, those that form large, star-shaped cysts. We report the isolation and characterization of 7 new Brazilian environmental Acanthamoeba isolates, all assigned to group I. Phylogenetic analyses based on partial (similar to 1200 bp) SSU rRNA gene sequences placed the new isolates in the robustly supported clade composed of the species of morphological group I. One of the Brazilian isolates is closely related to A. comandoni (genotype T9), while the other 6, together with 2 isolates recently assigned to genotype T17, form a homogeneous, well-supported group (2-0% sequence divergence) that likely represents a new Acanthamoeba species. Thermotolerance, osmotolerance, and cytophatic effects, features often associated with pathogenic potential, were also examined. The results indicated that all 7 Brazilian isolates grow at temperatures up to 40 degrees C, and resist under hvperosmotic conditions. Additionally, media conditioned by each of the new Acanthamoeba isolates induced the disruption of SIRC and HeLa cell monolayers.

A new day for the colored woman worker: a study of colored women in industry in New York City

Joint Committee to Study the Employment of Colored Women in New York City and Brooklyn.

Railroads, New York and New England, 1849 (Raster Image)

This layer is a georeferenced raster image of the historic paper map entitled: Map exhibiting the rail road, canal, lake and river routes from New York and Boston to the west, via Ogdensburgh, Buffalo and Sacket's Harbor, N.Y. It was published by J.H. Bufford & Co. Lith. in 1849. Scale [ca. 1:1,150,000]. The image inside the map neatline is georeferenced to the surface of the earth and fit to the Universal Transverse Mercator (UTM) Zone 18N NAD83 projection. All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, index maps, legends, or other information associated with the principal map. This map shows features such as roads, railroads (completed and proposed), canals, drainage, selected cities and towns, state boundaries, and more. Relief is shown by hachures. Includes table of statistics and distances, and note on "Advantages of the Sackets Harbor route... ." This layer is part of a selection of digitally scanned and georeferenced historic maps from The Harvard Map Collection as part of the Imaging the Urban Environment project. Maps selected for this project represent major urban areas and cities of the world, at various time periods. These maps typically portray both natural and manmade features at a large scale. The selection represents a range of regions, originators, ground condition dates, scales, and purposes.

Annual report - Superintendent of Banks, State of New York.

Some vols. issued with: Detailed statement.

Transactions of the Medical Society of the State of New York.

Lists of members in each volume; some of these are merely partial lists, members in attendance at the annual meetings, etc.

Catalogue of the Teachers' Library of the State of New York.

Mode of access: Internet.

Reports of decisions of the Public service commission, First district, of the state of New York /

Volume IX-XII, July 1-December, 1918-January 1-April 25, 1921 issued in combined report. Found also in the First annual report of the Public service commission, 1921 (New York (state) Public service commission)

A history of the Metropolitan Museum of Art, with a chapter on the early institutions of art in New York,

Prepared by Miss Howe under the direction and with the collaboration of Henry W. Kent. cf. Foreword, v. 1.

A summary of experimental concrete pavements in New York. Final report.

Federal Highway Administration, Office of Research, Development, and Technology, Washington, D.C.

New York Constitutional Convention index : a complete record of all proposed amendments introduced in the Convention beginning April 6, 1915.

"From April 6 to September 10, 1915."

The vegetables of New York ...

Vol. I, pt. I- issued as a report of the New York Agricultural Experiment Station at Geneva for the year ending June 30, 1928;June 30, 1931; June 30, 1934

Preliminary results of hydrologic studies at two recharge basins on Long Island, New York.

Prepared in cooperation with the New York State, Division of Water Resources, and others.

New York daguerreotyped : group first, business-streets, mercantile blocks, stores, and banks.

Illustrated with engravings from daguerreotypes.

A home for all, or, The gravel wall and octagon mode of building : new, cheap, convenient, superior and adapted to rich and poor ... /

Mode of access: Internet.

General index to the documents of the state of New York, from 1777 to 1865 inclusive.

Mode of access: Internet.