Avaliação toxicológica de óleo de nim sobre minhocas Eiseniaandrei

2015

Óleo de nim (Azadirachta indica) estimula o crescimento micelial de agentes de biocontrole.

2008

Projecto, montagem e teste de uma unidade modular de detecção de radiação Geiger-Muller

Dissertação para obtenção do grau de Mestre em Engenharia Electrotécnica Ramo de Automação e Electrónica Industrial

Novel Prostate Specific Antigen plastic antibody designed withcharged binding sites for an improved protein binding and itsapplication in a biosensor of potentiometric transduction

This work shows that the synthesis of protein plastic antibodies tailored with selected charged monomersaround the binding site enhances protein binding. These charged receptor sites are placed over a neutralpolymeric matrix, thus inducing a suitable orientation the protein reception to its site. This is confirmed bypreparing control materials with neutral monomers and also with non-imprinted template. This concepthas been applied here to Prostate Specific Antigen (PSA), the protein of choice for screening prostate can-cer throughout the population, with serum levels >10 ng/mL pointing out a high probability of associatedcancer.Protein Imprinted Materials with charged binding sites (C/PIM) have been produced by surfaceimprinting over graphene layers to which the protein was first covalently attached. Vinylben-zyl(trimethylammonium chloride) and vinyl benzoate were introduced as charged monomers labellingthe binding site and were allowed to self-organize around the protein. The subsequent polymerizationwas made by radical polymerization of vinylbenzene. Neutral PIM (N/PIM) prepared without orientedcharges and non imprinted materials (NIM) obtained without template were used as controls.These materials were used to develop simple and inexpensive potentiometric sensor for PSA. Theywere included as ionophores in plasticized PVC membranes, and tested over electrodes of solid or liq-uid conductive contacts, made of conductive carbon over a syringe or of inner reference solution overmicropipette tips. The electrodes with charged monomers showed a more stable and sensitive response,with an average slope of -44.2 mV/decade and a detection limit of 5.8 × 10−11mol/L (2 ng/mL). The cor-responding non-imprinted sensors showed lower sensitivity, with average slopes of -24.8 mV/decade.The best sensors were successfully applied to the analysis of serum, with recoveries ranging from 96.9to 106.1% and relative errors of 6.8%.

Graphene-based biomimetic materials targeting urine metabolite as potential cancer biomarker: Application over different conductive materials for potentiometric transduction

This work presents a novel surface Smart Polymer Antibody Material (SPAM) for Carnitine (CRT, a potential biomarker of ovarian cancer), tested for the first time as ionophore in potentiometric electrodes of unconventional configuration. The SPAM material consisted of a 3D polymeric network created by surface imprinting on graphene layers. The polymer was obtained by radical polymerization of (vinylbenzyl) trimethylammonium chloride and 4-styrenesulfonic acid (signaling the binding sites), and vinyl pivalate and ethylene glycol dimethacrylate (surroundings). Non-imprinted material (NIM) was prepared as control, by excluding the template from the procedure. These materials were then used to produce several plasticized PVC membranes, testing the relevance of including the SPAM as ionophore, and the need for a charged lipophilic additive. The membranes were casted over solid conductive supports of graphite or ITO/FTO. The effect of pH upon the potentiometric response was evaluated for different pHs (2-9) with different buffer compositions. Overall, the best performance was achieved for membranes with SPAM ionophore, having a cationic lipophilic additive and tested in HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) buffer, pH 5.1. Better slopes were achieved when the membrane was casted on conductive glass (-57.4 mV/decade), while the best detection limits were obtained for graphite-based conductive supports (3.6 × 10−5mol/L). Good selectivity was observed against BSA, ascorbic acid, glucose, creatinine and urea, tested for concentrations up to their normal physiologic levels in urine. The application of the devices to the analysis of spiked samples showed recoveries ranging from 91% (± 6.8%) to 118% (± 11.2%). Overall, the combination of the SPAM sensory material with a suitable selective membrane composition and electrode design has lead to a promising tool for point-of-care applications.

A biomimetic sensor for monitoring oxidative stress biomarker in point-of-care

1st ASPIC International Congress

Backside-surface imprinting as a new strategy to generate specific plastic antibody materials

A backside protein-surface imprinting process is presented herein as a novel way to generate specific synthetic antibody materials. The template is covalently bonded to a carboxylated-PVC supporting film previously cast on gold, let to interact with charged monomers and surrounded next by another thick polymer. This polymer is then covalently attached to a transducing element and the backside of this structure (supporting film plus template) is removed as a regular “tape”. The new sensing layer is exposed after the full template removal, showing a high density of re-binding positions, as evidenced by SEM. To ensure that the templates have been efficiently removed, this re-binding layer was cleaned further with a proteolytic enzyme and solution washout. The final material was named MAPS, as in the back-side reading of SPAM, because it acts as a back-side imprinting of this recent approach. It was able to generate, for the first time, a specific response to a complex biomolecule from a synthetic material. Non-imprinted materials (NIMs) were also produced as blank and were used as a control of the imprinting process. All chemical modifications were followed by electrochemical techniques. This was done on a supporting film and transducing element of both MAPS and NIM. Only the MAPS-based device responded to oxLDL and the sensing layer was insensitive to other serum proteins, such as myoglobin and haemoglobin. Linear behaviour between log(C, μg mL−1) versus charged tranfer resistance (RCT, Ω) was observed by electrochemical impedance spectroscopy (EIS). Calibrations made in Fetal Calf Serum (FCS) were linear from 2.5 to 12.5 μg mL−1 (RCT = 946.12 × log C + 1590.7) with an R-squared of 0.9966. Overall, these were promising results towards the design of materials acting close to the natural antibodies and applied to practical use of clinical interest.

Smart Plastic Antibody Material (SPAM) tailored on disposable screen printed electrodes for protein recognition: application to Myoglobin detection

This work introduces two major changes to the conventional protocol for designing plastic antibodies: (i) the imprinted sites were created with charged monomers while the surrounding environment was tailored using neutral material; and (ii) the protein was removed from its imprinted site by means of a protease, aiming at preserving the polymeric network of the plastic antibody. To our knowledge, these approaches were never presented before and the resulting material was named here as smart plastic antibody material (SPAM). As proof of concept, SPAM was tailored on top of disposable gold-screen printed electrodes (Au-SPE), following a bottom-up approach, for targeting myoglobin (Myo) in a point-of-care context. The existence of imprinted sites was checked by comparing a SPAM modified surface to a negative control, consisting of similar material where the template was omitted from the procedure and called non-imprinted materials (NIMs). All stages of the creation of the SPAM and NIM on the Au layer were followed by both electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). AFM imaging was also performed to characterize the topography of the surface. There are two major reasons supporting the fact that plastic antibodies were effectively designed by the above approach: (i) they were visualized for the first time by AFM, being present only in the SPAM network; and (ii) only the SPAM material was able to rebind to the target protein and produce a linear electrical response against EIS and square wave voltammetry (SWV) assays, with NIMs showing a similar-to-random behavior. The SPAM/Au-SPE devices displayed linear responses to Myo in EIS and SWV assays down to 3.5 μg/mL and 0.58 μg/mL, respectively, with detection limits of 1.5 and 0.28 μg/mL. SPAM materials also showed negligible interference from troponin T (TnT), bovine serum albumin (BSA) and urea under SWV assays, showing promising results for point-of-care applications when applied to spiked biological fluids.

Sistemas agroflorestais em áreas de agricultores familiares em Igarapé-Açu, Pará: caracterização florística, implantação e manejo

Este trabalho objetivou avaliar os Sistemas Agroflorestais-SAF comerciais multiestratificados de agricultores familiares de Igarapé-Açu, bem como identificar as espécies que compõem os sistemas agroflorestais. A partir de entrevistas foram catalogadas as espécies de maior interesse dos agricultores familiares e por meio de inventário florístico foram avaliados os SAF produtivos. Os cultivos de pimenta-do-reino, mandioca, feijão, cupuaçu, caju, açaí, pupunha, mogno e o nim indiano foram os mais freqüentes pelos agricultores. Os SAF são manejados na sua maioria de forma tradicional.

Caracterización de juegos basados en agentes móviles: diseño e implementación de un caso práctico y su incorporación en el programa docente de una asignatura de redes de computadores

En aquest projecte s'han classificat i posteriorment caracteritzat els jocs on la tecnologia d'agents mòbils és més òptima. Posteriorment s'ha dissenyat un core per al desenvolupament de jocs d'aquesta tipologia. Mitjançant aquest core s'han implementat el joc del Parchís i el joc del Nim. Aquest últim cas pràctic ha estat realitzat per a l'elaboració d'una pràctica per a l'assignatura de Xarxes de Computadors II. Gràcies als avantatges que ens oferix la tecnologia d'agents mòbils, com la tolerància a fallades i flexibilitat, hem demostrat amb aquest projecte que és òptim utilitzar aquesta tecnologia per a la implementació de jocs.

La successió intestada

El treball aprofundeix en la manera que es resol, a Catalunya, el destí dels béns de les persones que moren sense designar hereu perquè no fan testament o perquè aquest no és vàlid. I també estudia, amb detall, la forma en que està previst que els rebi la Generalitat de Catalunya, si no hi ha cap altre hereu. Es confirma que la successió intestada no és més que una alternativa residual a la fonamental, que és la designació d’hereu mitjançant testament. I també s’indica el destí dels béns integrants de les herències intestades que, segons la legislació vigent, s’adjudiquen a institucions sense ànim de lucre

Personalitat de la Fundació. Notes sobre la nova regulació a Catalunya

És evident el traspàs per part de l’Estat del Benestar de diferents accions socials a la societat civil, que aglutina un conjunt d’entitats sense ànim de lucre anomenat actualment com a tercer sector o sector privat social que es configura com un nou espai que floreix com una nova realitat a l’economia occidental, on desenvolupen un paper important en la prestació de serveis socials. En aquest context, el present treball pretén en primer lloc conèixer la concepció actual de persones jurídiques i desgranar-ne les categories. Fer una breu visió de l’evolució històrica d’aquestes entitats fins arribar a la configuració actual de les mateixes. D’aquestes entitats, les Fundacions són un dels membres més antics, genuïns i actius del tercer sector i per aquest motiu, declinem la segona part del present treball en l’estudi de la seva regulació a Catalunya, fent un anàlisi crític de la regulació de les Fundacions que conté el Llibre III del Codi Civil Català. Es qüestiona si la configuració jurídica de la Fundació i el sistema de control administratiu sobre les mateixes està en concordança amb les característiques i funcions actuals de les fundacions arrel dels escàndols financers d’una de les entitats més representatives de Catalunya, el Palau de la Música Catalana. En la mateixa línia s’analitzaran les exempcions fiscals actuals per a determinar si són suficients i adients per a incentivar la seva proliferació en el nostre teixit social.

LA DIRECCIÓ DE COMUNICACIÓ EN EL TERCER SECTOR

El Tercer Sector cívicosocial, entès com aquell conjunt d’organitzacions privades sense ànim de lucre que tenen com objectiu aconseguir la promoció de la persona, reduir les desigualtats socioeconòmiques i evitar l’exclusició social en el nostre territori, ha adquirit un pes molt significatiu en la nostra societat. És degut a aquesta importància, aconseguida al llarg dels anys, que totes les organitzaciones, fundacions i associacions han pres com a element de gran importància la comunicació. Saber comunicar-se ha arribat a un nivell imprescindible dins de la nostra societat i d’aquesta manera, el Tercer Sector n’ha pres consciència d’aquest fet. El present Treball Final de Carrera consisteix en l’estudi de diverses entitats representatives dels diversos sectors dins de l’àmbit territorial de Catalunya per veure de quina manera fan servir la comunicació i com es troben organitzades per saber assolir els seus objectius. Les nou entitats contactades són: Càritas, Intermón, Creu Roja, Amnistia Internacional, Greenpeace, Sos Racisme, SCIAS/ASC, Agrupació i ONCE. D’aquesta manera podem veure si el fet de disposar d’un departament dedicat a la comunicació, o de desenvolupar estratègies, repercuteix a obtenir uns millors resultats, tant interns com externs, dins de la entitat.

La virtud de la fortaleza : claves para superar la adversidad y para la prevención de la neurosis

En aquest treball tractarem la virtut de la fortalesa o la Força com a recurs de personalitat per la formació de personalitats equilibrades. Per l’estudi d’aquesta virtut ens basarem funamentalment en l’ensenyament Sant Tomàs d´Aquino i comprendrem cóm amb una ànim fort i capaç de suportar les adversitats d’acord al be difícil presentat per la recta raó, es pot arribar a superar els obstacles capaços de trencar l’estabilitat emocional i afectiva de la persona.

Classificació dels projectes informàtics i les EDT's

Aquest projecte tracta de recopilar tots els conceptes, criteris i característiques que es poden aplicar a un projecte amb l'ànim de que els lliuraments de projectes siguin fidels i es corresponguin amb la pràctica i realitat del servei o producte que es desitgi desenvolupar. Per garantir l'èxit ens centrarem en el disseny, planificació i visualització dels projectes fent ús de les WBS (Work Breakdown Structure) o EDT (Estructura del treball). Aquesta tècnica permet a l'equip de projecte exposar esquemàticament quin és el seu abast i socialitzar-les entre els diversos actors o persones involucrades de manera clara i esquematitzada.