Espacialização das estimativas de contaminação de águas subterrâneas por Tebuthiuron

Improper use of pesticides can lead to significant environmental impacts such as, contamination of environmental compartments, being the aquatic compartments the most vulnerable. In this context, the spatialization of pesticides concentrations estimative in groundwater provides important insights for decision making in managing and monitoring pesticides use. This study aimed to spatialize estimatives of groundwater contamination by Tebuthiuron, from different irrigation depths in the Rio Pardo basin, Pardinho-SP, Brazil. The simulations were performed using the ARAquá computer system, considering 0mm, 200 mm and 400 mm annual irrigation depths. Geostatistical techniques were used to obtain the spatial distribution of the simulated estimative. Tebuthiuron maps estimating concentration in groundwater were obtained by Kriging interpolation method, and indicated the areas with high potential for groundwater contamination. Considering all the simulations, it was concluded that there was no risk of groundwater contamination by Tebuthiuron in the study area.

Seleção de informações de guia de rota para a concepção de sistemas de navegação

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Militante... Já viu, né? A homofobia nos processos de subjetivação dos militantes do movimento LGBT

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Carta de sensibilidade ambiental para derramamentos de óleo em rodovias: proposta aplicada a Estrada dos Tamoios (SP-099)

Pós-graduação em Geociências e Meio Ambiente - IGCE

Conflitos por múltiplos usos da água no reservatório da UHE de Tucuruí

Os conflitos por múltiplos usos da água, no reservatório da UHE Tucuruí, surgem com o anúncio da construção da mega hidrelétrica na região, e os conflitos socioambientais subseqüentes. Aliada a elevação do nível de percepção social em relação aos problemas ambientais cresce também a busca por eficientes processos de gestão e gerenciamento de recursos hídricos. Este estudo objetiva analisar e tipificar os conflitos por múltiplos usos da água no reservatório da UHE Tucuruí, utilizando-se como ferramenta de apoio à decisão o software de modelagem qualitativa NVivo 8, e dessa forma verificar as melhores alternativas a serem adotadas para a conciliação dos usos múltiplos no reservatório. As tipificações realizadas basearam-se na análise dos conflitos, seus componentes, elementos e aspectos, tipo, natureza e origem. Sendo assim, identificaram-se três principais tipos de conflitos no reservatório da UHE Tucuruí: conflitos entre distintos grupos de usuários da água, conflitos por obras hidráulicas e conflitos decorrentes de poluição ambiental. Para este estudo adotou - se uma abordagem qualitativa, através do método de mapeamento cognitivo. Este tipo de mapeamento possibilitou a construção de um modelo cognitivo para a gestão dos conflitos no reservatório de Tucuruí. Sendo assim, o software NVivo 8 possibilitou, além da análise dos dados obtidos nas entrevistas e no levantamento bibliográfico, a construção do modelo gráfico de apoio à gestão de conflitos por múltiplos usos da água. Verificou-se que uma das formas de solução dos conflitos é através da análise destes, em vistas de se investigar os mecanismos adequados para sua resolução, e posterior proposição de medidas estruturais e/ou nãoestruturais para a gestão de recursos hídricos. As principais ações para a solução dos conflitos estão enquadradas nos métodos de resolução institucional de longo prazo. O modelo pode funcionar como suporte ao planejamento e tomada de decisão, tendo em vista os problemas ambientais, a participação dos usuários da água no sistema hídrico, as políticas públicas, e também a gestão integrada dos recursos hídricos. Concluiu- se, então, que a exploração dos recursos hídricos deve proporcionar os múltiplos usos da água em atendimento aos princípios da sustentabilidade ambiental, inseridos num processo de gestão dos conflitos por múltiplos usos da água e gerenciamento integrado dos recursos hídricos.

Mapeamento de magnetização aparente usando regularização entrópica

Apresentamos um novo método de mapeamento de magnetização aparente no plano horizontal que combina a minimização da entropia de primeira ordem com a maximização da entropia de ordem zero dos contrastes de magnetização estimados. O modelo interpretativo é uma malha de prismas verticais justapostos ao longo de ambas as direções horizontais. Presumimos que o topo e a base das fontes magnéticas são planos e horizontais e estimamos os contrastes de magnetização dos prismas. A minimização da entropia de primeira ordem favorece soluções de bordas abruptas e a maximização da entropia de ordem zero evita a tendência de a fonte estimada ser um único prisma. Desta forma, uma combinação judiciosa de ambos os vínculos pode levar a soluções caracterizadas por regiões de contraste de magnetização virtualmente constantes separadas por descontinuidades abruptas. Aplicamos este método a dados sintéticos produzidos por intrusões simuladas em sedimentos e que apresentam topo e base planos e horizontais. Comparando nossos resultados com aqueles obtidos pelo vínculo da suavidade, mostramos que ambos os métodos produzem uma boa e equivalente localização do centro das fontes. Todavia, a regularização entrópica delineia as bordas do corpo com maior detalhe. Ambos os vínculos (suavidade e regularização entrópica) foram aplicados a uma anomalia real sobre um escarnito magnético em Butte Valley, Nevada, Estados Unidos. A regularização entrópica produziu uma estimativa da distribuição de magnetização com bordas mais abruptas, menor volume e maiores valores de magnetização aparente comparados àqueles produzidos pelo vinculo da suavidade.

Psicologia e sistema penitenciário: cartografando as atuações da(o)s psicóloga(o)s em uma "instituição total"

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Identificazione di un QTL principale per resistenza a ruggine bruna sul cromosoma 7B di frumento duro

Leaf rust caused by Puccinia triticina is a serious disease of durum wheat (Triticum durum) worldwide. However, genetic and molecular mapping studies aimed at characterizing leaf rust resistance genes in durum wheat have been only recently undertaken. The Italian durum wheat cv. Creso shows a high level of resistance to P. triticina that has been considered durable and that appears to be due to a combination of a single dominant gene and one or more additional factors conferring partial resistance. In this study, the genetic basis of leaf rust resistance carried by Creso was investigated using 176 recombinant inbred lines (RILs) from the cross between the cv. Colosseo (C, leaf rust resistance donor) and Lloyd (L, susceptible parent). Colosseo is a cv. directly related to Creso with the leaf rust resistance phenotype inherited from Creso, and was considered as resistance donor because of its better adaptation to local (Emilia Romagna, Italy) cultivation environment. RILs have been artificially inoculated with a mixture of 16 Italian P. triticina isolates that were characterized for virulence to seedlings of 22 common wheat cv. Thatcher isolines each carrying a different leaf rust resistance gene, and for molecular genotypes at 15 simple sequence repeat (SSR) loci, in order to determine their specialization with regard to the host species. The characterization of the leaf rust isolates was conducted at the Cereal Disease Laboratory of the University of Minnesota (St. Paul, USA) (Chapter 2). A genetic linkage map was constructed using segregation data from the population of 176 RILs from the cross CL. A total of 662 loci, including 162 simple sequence repeats (SSRs) and 500 Diversity Arrays Technology markers (DArTs), were analyzed by means of the package EasyMap 0.1. The integrated SSR-DArT linkage map consisted of 554 loci (162 SSR and 392 DArT markers) grouped into 19 linkage blocks with an average marker density of 5.7 cM/marker. The final map spanned a total of 2022 cM, which correspond to a tetraploid genome (AABB) coverage of ca. 77% (Chapter 3). The RIL population was phenotyped for their resistance to leaf rust under artificial inoculation in 2006; the percentage of infected leaf area (LRS, leaf rust susceptibility) was evaluated at three stages through the disease developmental cycle and the area under disease progress curve (AUDPC) was then calculated. The response at the seedling stage (infection type, IT) was also investigated. QTL analysis was carried out by means of the Composite Interval Mapping method based on a selection of markers from the CL map. A major QTL (QLr.ubo-7B.2) for leaf rust resistance controlling both the seedling and the adult plant response, was mapped on the distal region of chromosome arm 7BL (deletion bin 7BL10-0.78-1.00), in a gene-dense region known to carry several genes/QTLs for resistance to rusts and other major cereal fungal diseases in wheat and barley. QLr.ubo-7B.2 was identified within a supporting interval of ca. 5 cM tightly associated with three SSR markers (Xbarc340.2, Xgwm146 e Xgwm344.2), and showed an R2 and an LOD peak value for the AUDPC equal to 72.9% an 44.5, respectively. Three additional minor QTLs were also detected (QLr.ubo-7B.1 on chr. 7BS; QLr.ubo-2A on chr. 2AL and QLr.ubo-3A on chr. 3AS) (Chapter 4). The presence of the major QTL (QLr.ubo-7B.2) was validated by a linkage disequilibrium (LD)-based test using field data from two different plant materials: i) a set of 62 advanced lines from multiple crosses involving Creso and his directly related resistance derivates Colosseo and Plinio, and ii) a panel of 164 elite durum wheat accessions representative of the major durum breeding program of the Mediterranean basin. Lines and accessions were phenotyped for leaf rust resistance under artificial inoculation in two different field trials carried out at Argelato (BO, Italy) in 2006 and 2007; the durum elite accessions were also evaluated in two additional field experiments in Obregon (Messico; 2007 and 2008) and in a green-house experiment (seedling resistance) at the Cereal Disease Laboratory (St. Paul, USA, 2008). The molecular characterization involved 14 SSR markers mapping on the 7BL chromosome region found to harbour the major QTL. Association analysis was then performed with a mixed-linear-model approach. Results confirmed the presence of a major QTL for leaf rust resistance, both at adult plant and at seedling stage, located between markers Xbarc340.2, Xgwm146 and Xgwm344.2, in an interval that coincides with the supporting interval (LOD-2) of QLr.ubo-7B.2 as resulted from the RIL QTL analysis. (Chapter 5). The identification and mapping of the major QTL associated to the durable leaf rust resistance carried by Creso, together with the identification of the associated SSR markers, will enhance the selection efficiency in durum wheat breeding programs (MAS, Marker Assisted Selection) and will accelerate the release of cvs. with durable resistance through marker-assisted pyramiding of the tagged resistance genes/QTLs most effective against wheat fungal pathogens.

Molecular strategies for genetic diversity analysis and development of markers linked to resistance traits in apple

The goal of many plant scientists’ research is to explain natural phenotypic variation in term of simple changes in DNA sequence. DNA-based molecular markers are extensively used for the construction of genome-wide molecular maps and to perform genetic analysis for simple and complex traits. The PhD thesis was divided into two main research lines according to the different approaches adopted. The first research line is to analyze the genetic diversity in an Italian apple germplasm collection for the identification of markers tightly linked to targeted genes by an association genetic method. This made it possible to identify synomym and homonym accessions and triploids. The fruit red skin color trait has been used to test the reliability of the genetic approaches in this species. The second line is related to the development of molecular markers closely linked to the Rvi13 and Rvi5 scab resistance genes, previously mapped on apple’s chromosome 10 and 17 respectively by using the traditional linkage mapping method. Both region have been fine-mapped with various type of markers that could be used for marker-assisted selection in future breeding programs and to isolate the two resistance genes.

Identification and characterization of the herpes simplex virus type 2 gene encoding the essential capsid protein ICP32/VP19c.

We describe the characterization of the herpes simplex virus type 2 (HSV-2) gene encoding infected cell protein 32 (ICP32) and virion protein 19c (VP19c). We also demonstrate that the HSV-1 UL38/ORF.553 open reading frame (ORF), which has been shown to specify a viral protein essential for capsid formation (B. Pertuiset, M. Boccara, J. Cebrian, N. Berthelot, S. Chousterman, F. Puvian-Dutilleul, J. Sisman, and P. Sheldrick, J. Virol. 63: 2169-2179, 1989), must encode the cognate HSV type 1 (HSV-1) ICP32/VP19c protein. The region of the HSV-2 genome deduced to contain the gene specifying ICP32/VP19c was isolated and subcloned, and the nucleotide sequence of 2,158 base pairs of HSV-2 DNA mapping immediately upstream of the gene encoding the large subunit of the viral ribonucleotide reductase was determined. This region of the HSV-2 genome contains a large ORF capable of encoding two related 50,538- and 49,472-molecular-weight polypeptides. Direct evidence that this ORF encodes HSV-2 ICP32/VP19c was provided by immunoblotting experiments that utilized antisera directed against synthetic oligopeptides corresponding to internal portions of the predicted polypeptides encoded by the HSV-2 ORF or antisera directed against a TrpE/HSV-2 ORF fusion protein. The type-common immunoreactivity of the two antisera and comparison of the primary amino acid sequences of the predicted products of the HSV-2 ORF and the equivalent genomic region of HSV-1 provided evidence that the HSV-1 UL38 ORF encodes the HSV-1 ICP32/VP19c. Analysis of the expression of the HSV-1 and HSV-2 ICP32/VP19c cognate proteins indicated that there may be differences in their modes of synthesis. Comparison of the predicted structure of the HSV-2 ICP32/VP19c protein with the structures of related proteins encoded by other herpes viruses suggested that the internal capsid architecture of the herpes family of viruses varies substantially.

Organisation of the equine immunoglobulin constant heavy chain genes. II. Equine cgamma genes.

The number of immunoglobulin G constant heavy chain genes (cgamma genes) varies broadly among mammalian species, reflecting structural and functional differences between expressed immunoglobulin G (IgG) isotypes and allotypes. Up to now equine IgG isotypes have been defined only at the biochemical and serological level. It is still not clear how many IgG isotypes exist in horses and whether there are any allotypes. Here, we describe the isolation and characterisation of equine cgamma genes. An equine genomic lambda phage library was screened with a human cgamma4 probe. Cross-hybridising equine cgamma sequences were cloned twice and characterised by restriction mapping with the human cgamma4 and a murine sgamma1 probe. Genomic equine DNA probes for both, cgamma genes and corresponding switch regions (sgamma), were isolated and used for a more detailed BamHI restriction analysis, comparing genomic DNA of various horses. This analysis reveals the existence of at least five, or probably six cgamma genes in the equine haploid genome. Beside the porcine system, this is the highest number of cgamma genes described for any mammalian species. Moreover, for two of these cgamma genes, BamHI restriction fragment length polymorphism became evident.

GLUCAGON GENE EXPRESSION: ANALYSIS OF PREPROGLUCAGON

Glucagon is a 29 amino acid polypeptide hormone produced in the (alpha) cells of the pancreatic islets. The purpose of this research was to understand better the role of glucagon in the regulation of metabolic processes. As with other polypeptide hormones, the synthesis of glucagon is thought to involve a larger precursor, which is then enzymatically cleaved to the functional form. The specific research objectives were to obtain cloned copies of the messenger RNA (mRNA) for pancreatic glucagon, to determine their primary sequences, and from this coding information to deduce the amino acid sequence of the initial glucagon precursor. From this suggested preproglucagon sequence and prior information on possible proglucagon intermediate processing products, the overall objective of this research is to propose a possible pathway for the biosynthesis of pancreatic glucagon.^ Synthetic oligodeoxynucleotide probes of 14-nucleotides (14-mer) and 17-nucleotides (a 17-mer) complementary to codons specifying a unique sequence of mature glucagon were synthesized. The ('32)P-labeled-14-mer was hybridized with size-fractionated fetal bovine pancreatic poly(A('+))RNA bound to nitrocellulose. RNA fractions of (TURN)14S were found to hybridize specifically, resulting in an (TURN)10-fold enrichment for these sequences. These poly(A('+))RNAs were translated in a cell-free system and the products analyzed by gel electrophoresis. The translation products were found to be enriched for a protein of the putative size of mammalian preproglucagon ((TURN)21 kd). These enriched RNA fractions were used to construct a complementary DNA (cDNA) library is plasmid pBR322.^ Screening of duplicate colony filters with the ('32)P-labeled-17-mer and a ('32)P-labeled-17-mer-primed cDNA probe indicated 25 possible glucagon clones from 3100 colonies screened. Restriction mapping of 6 of these clones suggested that they represented a single mRNA species. Primary sequence analysis of one clone containing a 1200 base pair DNA insert revealed that it contained essentially a full-length copy of glucagon cDNA.^ Analaysis of the cDNA suggested that it encoded an initial translation product of 180 amino acids with an M(,r) = 21 kd. The first initiation codon (ATG, methionine) followed by the longest open reading frame of 540 nucleotides was preceded by a 5'-untranslated region of 90 nucleotides, and was followed by a longer 3'-untranslated region of 471 nucleotides, resulting in a total of 1101 nucleotides. . . . (Author's abstract exceeds stipulated maximum length. Discontinued here with permission of author.) UMI ^

MATHEMATICAL STUDIES ON THE EVOLUTIONARY CHANGE OF DNA SEQUENCES

With the aim of understanding the mechanism of molecular evolution, mathematical problems on the evolutionary change of DNA sequences are studied. The problems studied and the results obtained are as follows: (1) Estimation of evolutionary distance between nucleotide sequences. Studying the pattern of nucleotide substitution for the case of unequal substitution rates, a new mathematical formula for estimating the average number of nucleotide substitutions per site between two homologous DNA sequences is developed. It is shown that this formula has a wider applicability than currently available formulae. A statistical method for estimating the number of nucleotide changes due to deletion and insertion is also developed. (2) Biases of the estimates of nucleotide substitutions obtained by the restriction enzyme method. The deviation of the estimate of nucleotide substitutions obtained by the restriction enzyme method from the true value is investigated theoretically. It is shown that the amount of the deviation depends on the nucleotides in the recognition sequence of the restriction enzyme used, unequal rates of substitution among different nucleotides, and nucleotide frequences, but the primary factor is the unequal rates of nucleotide substitution. When many different kinds of enzymes are used, however, the amount of average deviation is generally small. (3) Distribution of restriction fragment lengths. To see the effect of undetectable restriction fragments and fragment differences on the estimate of nucleotide differences, the theoretical distribution of fragment lengths is studied. This distribution depends on the type of restriction enzymes used as well as on the relative frequencies of four nucleotides. It is shown that undetectability of small fragments or fragment differences gives a serious underestimate of nucleotide substitutions when the length-difference method of estimation is used, but the extent of underestimation is small when the site-difference method is used. (4) Evolutionary relationships of DNA sequences in finite populations. A mathematical theory on the expected evolutionary relationships among DNA sequences (nucleons) randomly chosen from the same or different populations is developed under the assumption that the evolutionary change of nucleons is determined solely by mutation and random genetic drift. . . . (Author's abstract exceeds stipulated maximum length. Discontinued here with permission of author). UMI ^

GENETIC ORGANIZATION OF THE STRUCTURAL GENES FOR NITRATE REDUCTASE (TN5)

Nitrate reductase in Escherichia coli is a membrane-bound anaerobic enzyme that is repressed by oxygen and induced by nitrate. The genetic organization of the structural genes for the two larger subunits of nitrate reductase ((alpha) and (beta)) was determined by immunoprecipitation analysis of the formation of these proteins in nitrate reductase-deficient mutants resulting from transposon Tn5 mutagenesis. The results suggested that the genes encoding the (alpha) and (beta) subunits (narG and H) were arranged in an operon with transcription in the direction promoter(--->)(alpha)(--->)(beta). Segments of the chromosome containing the Tn5 inserts from several of the mutants were cloned into plasmid pBR322 and the positions of the transposons determined by restriction mapping. The Tn5 insertion sites were localized on two contiguous EcoRI fragments spanning about 6.6 kilobases of DNA. The narI gene (proposed to encode the (gamma) subunit) was positioned immediately downstream from the (beta)-gene (narH) by Southern analysis of Tn10 insertions into the narI locus. A Tn10 insertion into the narK locus, proposed to encode a nitrate-sensitive repressor of other anaerobic enzymes, was located about 1.5 kilobases upstream from the narGHI operon promoter. The narL locus, proposed to encode a nitrate-sensitive positive regulator of the narGHI operon and known to be genetically linked to the other nar genes, was demonstrated to lie outside a 19.3-kilobase region of the chromosome which encompasses the other nar genes. The physical limit of the narGHI promoter was defined by studying the effect of Tn5 insertions into a hybrid plasmid containing the functional operon. The points of origin of the coding regions for the (alpha) and (beta) genes were deduced by alignment of the chromosomal map of Tn5 insertion sites with the sizes of (alpha) and (beta) subunit fragments produced by plasmids carrying these Tn5 inserts in the nar operon. The coding region for the (alpha) subunit (143,000 daltons) begins about 250 nucleotides downstream from the deduced limit of the promoter region and includes about 4.0 kilobases of DNA; the region encoding (beta) (60,000 daltons) lies immediately downstream from the (alpha)-gene and is approximately 1.6 kilobases in length. The adjacent region encoding the (gamma) subunit (19,000 daltons) is approximately 0.5 kilobase in length. ^

Aplicación de herramientas gis para la cartografía y correlación de datos de ensayos no destructivos en el diagnóstico de edificios históricos

La presente tesis propone un nuevo método de cartografía de ensayos no destructivos en edificios históricos mediante el uso de técnicas basadas en SIG. Primeramente, se define el método por el cual es posible elaborar y convertir una cartografía 3D basada en nubes de puntos de un elemento arquitectónico obtenida mediante fotogrametría, en cartografía raster y vectorial, legible por los sistemas SIG mediante un sistema de coordenadas particular que referencian cada punto de la nube obtenida por fotogrametría. A esta cartografía inicial la denominaremos cartografía base. Después, se define el método por el cual los puntos donde se realiza un ensayo NDT se referencian al sistema de coordenadas del plano base, lo que permite la generación de cartografías de los ensayos referenciadas y la posibilidad de obtener sobre un mismo plano base diferentes datos de múltiples ensayos. Estas nuevas cartografías las denominaremos cartografías de datos, y se demostrará la utilidad de las mismas en el estudio del deterioro y la humedad. Se incluirá el factor tiempo en las cartografías, y se mostrará cómo este nuevo hecho posibilita el trabajo interdisciplinar en la elaboración del diagnóstico. Finalmente, se generarán nuevas cartografías inéditas hasta entonces consistentes en la combinación de diferentes cartografías de datos con la misma planimetría base. Estas nuevas cartografías, darán pie a la obtención de lo que se ha definido como mapas de isograma de humedad, mapa de isograma de salinidad, factor de humedad, factor de evaporación, factor de salinidad y factor de degradación del material. Mediante este sistema se facilitará una mejor visión del conjunto de los datos obtenidos en el estudio del edificio histórico, lo que favorecerá la correcta y rigurosa interpretación de los datos para su posterior restauración. ABSTRACT This research work proposes a new mapping method of non-destructive testing in historical buildings, by using techniques based on GIS. First of all, the method that makes it possible to produce and convert a 3D map based on cloud points from an architectural element obtained by photogrammetry, are defined, as raster and vector, legible by GIS mapping systems using a particular coordinate system that will refer each cloud point obtained by photogrammetry. This initial mapping will be named base planimetry. Afterwards, the method by which the points where the NDT test is performed are referenced to the coordinate system of the base plane , which allows the generation of maps of the referenced tests and the possibility of obtaining different data from multiple tests on the same base plane. These new maps will be named mapping data and their usefulness will be demonstrated in the deterioration and moisture study. The time factor in maps will be included, and how this new fact will enable the interdisciplinary work in the elaboration of the diagnosis will be proved. Finally, new maps (unpublished until now) will be generated by combining different mapping from the same planimetry data base. These new maps will enable us to obtain what have been called isograma moisture maps, isograma salinity- maps, humidity factor, evaporation factor, salinity factor and the material degradation factor. This system will provide a better vision of all data obtained in the study of historical buildings , and will ease the proper and rigorous data interpretation for its subsequent restoration.