Preparative droplet counter-current chromatography for the separation of the new nor-seco-triterpene and pentacyclic triterpenoids from Qualea parviflora

The methanolic extract of the bark of the medicinal plant Qualea parviflora (Vochysiaceae) contains new nor-seco-triterpene and pentacyclic triterpenoids. They were separated in a preparative scale using droplet counter-current chromatography. The optimum solvent used was composed of a mixture of CHCl3/MeOH/H2O (43:37:20, v/v) in the descending mode and led to a successful separation of the new compound 28-nor-17, 22-seco-2 alpha, 3 beta, 19, 22, 23-pentahydroxy-Delta(12)-olecinane, besides the known triterpenoids bellericagenin B, bellericaside B and arjunglucoside I. Identification was performed by ESI-MS, H-1 NMR and C-13 NMR analyses.

Qualea parviflora Mart.: An integrative study to validate the gastroprotective, antidiarrheal, antihemorragic and mutagenic action

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Effect of gibberellic acid on the quality of chrysanthemum (Dendranthema grandiflora L.) cv. Faroe

The aim of the present work was to evaluate the quality of chrysanthemum cultivar Faroe, subjected to different gibberellic acid (GA(3)) concentrations applied to the field. The treatments were composed of four doses of GA(3) (0, 15, 30 and 45 mg L-1) applied at the beginning of the formation of buttom floral (28 days after transplanting of seedlings). According to the results, a single low concentration of GA(3) application did not interfere with the phenotypic characteristics of the chrysanthemum cultivar Faroe.

Morfologia e anatomia da semente de Esenbeckia grandiflora Mart. (Rutaceae)

Foram descritos e ilustrados aspectos morfo-anatômicos das sementes de Esenbeckia grandiflora Mart., visando o conhecimento dos tegumentos, endosperma e embrião. As sementes são de elipsóides a piramidais, marrom-escuras, anátropas, mesotestais, sem tégmem, exariladas e exalbuminosas. O embrião é axial, reto, total, branco, com cotilédones carnosos de reserva lipo-protéica, eixo hipocótilo-radícula curto e plúmula reduzida.

Homomorphic self-incompatibility, with stylar rejection site, in Luehea grandiflora Mart. and Zucc. (Tiliaceae-Malvaceae s.l.)

The breeding system of Luehea grandiflora (Tiliaceae-Malvaceae s.l.) was investigated using hand pollinations and fluorescence microscopy studies of pollen tube growth. Although selfed flowers persisted for some 10 days, our study indicates that L. grandiflora is self-incompatible, with self pollen tube inhibition in the upper style, as occurs in many taxa with homomorphic, gametophytic self-incompatibility (GSI). L. grandiflora is only the second species reported within the Malvales with homomorphic stylar inhibition. This result is discussed within the context of a report for self-compatibility in this species, and we also consider the phylogenetic implications for the occurrence of GSI in the family Malvaceae s.l.

Antioxidant flavonol glycosides from Nectandra grandiflora (Lauraceae)

O fracionamento cromatográfico do extrato etanólico das folhas de Nectandra grandiflora resultou no isolamento de dois flavonóides glicosilados que apresentaram atividade antioxidante inibindo a oxidação do beta -caroteno em CCDC. As substâncias isoladas tiveram suas estruturas elucidadas através de técnicas espectrométricas de RMN uni- e bidimensional e foram identificadas como 3-O-beta -ramnosy kaempferol and 3-O-beta -ramnosylquercetina.

Effect of storage of cuttings on the rooting of different cultivars of cut chrysanthemums (Dendranthema grandiflora Tzvelev) for two different seasons

Chrysanthemums are commercially propagated through cuttings. Pre-rooting storage of cuttings in the dark is a common practice among growers and companies that work and trade with chrysanthemum cuttings. Therefore, the maximum storage period for cuttings and differences in tolerance among cultivars has been investigated. Adventitious roots of cuttings can originate in almost any tissue, including the epidermis, stem cortex and pericycle, ray parenchyma, immature xylem and phloem cells, and pith. The aims of this work were to determine the effect of time of cold storage of cuttings (0, 1, 2, 3, 4, 5 and 6 weeks) on the rooting of four cut chrysanthemum cultivars (Super White, Sheena, Dark Orange Reagan and Town Talk) for two seasons of the year (summer and winter), and also to determine the origin of root formation in chrysanthemum cuttings. The study was carried out as a randomized complete block design with 5 replications for each storage treatment. Each plot was comprised of three cuttings that were examined 14 days after the cutting procedure. During the winter, the roots were studied by scanning electron microscopy (SEM) at the Electron Microscope Laboratory. The following conclusions were made: in winter, cold storage affected the rooting of cuttings, mainly after two weeks of storage for all cultivars. The rooting percentage was lower in the winter and the cuttings could be preserved for a shorter period. The source and growth of roots in chrysanthemum cuttings was found to be endogenous. After three days, callus formed in the pericycle and the first emergence of adventitious roots occurred by the fourth day of rooting. During the summer, cold storage could be up to 4 weeks without any problems.

A new neolignan and antioxidant phenols from Nectandra grandiflora

The EtOH crude extract from the leaves of Nectandra grandiflora collected in the Atlantic Forest, Brazil, showed antioxidant activity towards β-carotene in a TLC assay. The bioassay-guided fractionation led to the isolation of protocatechuic acid and two flavonol glycosides: afzelin and quercetrin, which showed free radical scavenging activity towards DPPH (ΔA 32, 16 and 73% at 50 μmol L-1) and were compared to commercial antioxidants rutin (81% at 50 μmol L-1) and BHT (9% at 50 μmol L-1), used as standard compounds. Additionally, three inactive neolignans, including the new bicycle[3.2.1]octane neolignan 2′-oxo-piperol B were obtained and characterized by spectrometric methods. ©2005 Sociedade Brasileira de Química.

Atividade antiinflamatória e antitumoral da fração terpenoídica e de -sitosterol obtidos de qualea multiflora

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Produção de mudas de duas espécies do gênero qualea utilizando água residuária, fertirrigação e irrigação por capilaridade

Pós-graduação em Agronomia (Irrigação e Drenagem) - FCA

Rendimento operacional de uma serraria com a espécie Cambará (Qualea albiflora Warm.) na região Amazônica

Pós-graduação em Ciência Florestal - FCA

Efeito dos componentes obtidos de qualea multiflora sobre modelo tumoral mamário murino e sua influência no sistema imunológico

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Effect of Qualea multiflora on Macrophages and Tumour Cells

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Antidiarrheal and intestinal antiinflammatory activities of a methanolic extract of Qualea parviflora Mart. in experimental models

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Avaliação da atividade antioxidante de Qualea parviflora sobre a doença inflamatória intestinal e a úlcera gástrica induzidas em roedores

Pós-graduação em Biologia Geral e Aplicada - IBB